Effect of remote ischaemic conditioning on clinical outcomes in patients with acute myocardial infarction (CONDI-2/ERIC-PPCI): a single-blind randomised controlled trial.

BACKGROUND: Remote ischaemic conditioning with transient ischaemia and reperfusion applied to the arm has been shown to reduce myocardial infarct size in patients with ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PPCI). We investigated whether remote ischaemic conditioning could reduce the incidence of cardiac death and hospitalisation for heart failure at 12 months. METHODS: We did an international investigator-initiated, prospective, single-blind, randomised controlled trial (CONDI-2/ERIC-PPCI) at 33 centres across the UK, Denmark, Spain, and Serbia. Patients (age >18 years) with suspected STEMI and who were eligible for PPCI were randomly allocated (1:1, stratified by centre with a permuted block method) to receive standard treatment (including a sham simulated remote ischaemic conditioning intervention at UK sites only) or remote ischaemic conditioning treatment (intermittent ischaemia and reperfusion applied to the arm through four cycles of 5-min inflation and 5-min deflation of an automated cuff device) before PPCI. Investigators responsible for data collection and outcome assessment were masked to treatment allocation. The primary combined endpoint was cardiac death or hospitalisation for heart failure at 12 months in the intention-to-treat population. This trial is registered with ClinicalTrials.gov (NCT02342522) and is completed. FINDINGS: Between Nov 6, 2013, and March 31, 2018, 5401 patients were randomly allocated to either the control group (n=2701) or the remote ischaemic conditioning group (n=2700). After exclusion of patients upon hospital arrival or loss to follow-up, 2569 patients in the control group and 2546 in the intervention group were included in the intention-to-treat analysis. At 12 months post-PPCI, the Kaplan-Meier-estimated frequencies of cardiac death or hospitalisation for heart failure (the primary endpoint) were 220 (8·6%) patients in the control group and 239 (9·4%) in the remote ischaemic conditioning group (hazard ratio 1·10 [95% CI 0·91-1·32], p=0·32 for intervention versus control). No important unexpected adverse events or side effects of remote ischaemic conditioning were observed. INTERPRETATION: Remote ischaemic conditioning does not improve clinical outcomes (cardiac death or hospitalisation for heart failure) at 12 months in patients with STEMI undergoing PPCI. FUNDING: British Heart Foundation, University College London Hospitals/University College London Biomedical Research Centre, Danish Innovation Foundation, Novo Nordisk Foundation, TrygFonden

Mécanismes impliqués dans la sélection génomique sur la résistance aux mammites

National audienc

Response to selection on mastitis resistance and body reserve mobilization in Holstein and Normande breeds

International audienceA divergent selection experiment on mastitis resistance of Holstein and Normande cows was carried out at Le Pin, the INRA experimental farm. Two lines, one resistant to mastitis and one control, were produced by using bulls with contrasted breeding values for a combination of somatic cell score (SCS) and clinical mastitis (CM). In Holstein, the same population was also split into two groups diverging for body condition score (BCS) and obtained by selecting the sires on their BCS breeding values. This paper presents the first results on the observed differences between lines based on data of 268 cows. Overall differences in SCS, CM, infectious status (assessed by bacteriological PCR tests) and BCS were in agreement with genomic predictions. Within-breed differences were similar, but sometimes not significant for udder health traits. These cows support extensive phenotyping for explanatory traits to explain the biological pathways involved in these differences

Réponse à une sélection divergente sur la résistance aux mammites et la mobilisation corporelle en races Holstein et Normande

National audienc

A divergent genetic selection experiment on mastitis resistance in Holstein and Normand breeds

Session 3 : Immunity and geneticsNational audienc

Response to selection on mastitis resistance and body reserve mobilization in Holstein and Normande breeds

International audienceA divergent selection experiment on mastitis resistance of Holstein and Normande cows was carried out at Le Pin, the INRA experimental farm. Two lines, one resistant to mastitis and one control, were produced by using bulls with contrasted breeding values for a combination of somatic cell score (SCS) and clinical mastitis (CM). In Holstein, the same population was also split into two groups diverging for body condition score (BCS) and obtained by selecting the sires on their BCS breeding values. This paper presents the first results on the observed differences between lines based on data of 268 cows. Overall differences in SCS, CM, infectious status (assessed by bacteriological PCR tests) and BCS were in agreement with genomic predictions. Within-breed differences were similar, but sometimes not significant for udder health traits. These cows support extensive phenotyping for explanatory traits to explain the biological pathways involved in these differences

A divergent genetic selection experiment on mastitis resistance in Holstein and Normand breeds

National audienc

Functional activation of T cells by dendritic cells and macrophages exposed to the intracellular parasite <em>Neospora</em> <em>caninum</em>

International audienc

Selection on mastitis resistance in Holstein and Normande breeds: genetic and immune responses

International audienc

Moins de mammites avec la génétique!

National audienc