DYSPROTEINAEMIA IN VIRAL HEPATITIS

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IMMUNOCHEMICAL QUANTITATIVE DETERMINATION OF SOME SERUM PROTEINS AND THEIR DIAGNOSTICAL IMPORTANCE

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Role of homologous ASP334 and GLU319 in human non-gastric H,K- and Na,K-ATPases in cardiac glycoside binding

Cardiac steroids inhibit Na,K-ATPase and the related non-gastric H,K-ATPase, while they do not interact with gastric H,K-ATPase. Introducing an arginine, the residue present in the gastric H,K-ATPase, in the second extracellular loop at the corresponding position 334 in the human non-gastric H,K-ATPase (D334R mutation) rendered it completely resistant to 2mM ouabain. The corresponding mutation (E319R) in alpha1 Na,K-ATPase produced a approximately 2-fold increase of the ouabain IC(50) in the ouabain-resistant rat alpha1 Na,K-ATPase and a large decrease of the ouabain affinity of human alpha1 Na,K-ATPase, on the other hand this mutation had no effect on the affinity for the aglycone ouabagenin. These results provide a strong support for the orientation of ouabain in its biding site with its sugar moiety interacting directly with the second extracellular loop

RELATION BETWEEN VARICELLA AND HERPES ZOSTER

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QUANTITATIVE CHANGES IN SOME SERUM PROTEINS IN VIRAL HEPATITIS

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INDIVIDUAL SERUM PROTEIN CONSTELLATIONS IN THE DIAGNOSIS AND PROGNOSIS OF VIRAL HEPATITIS

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UNVEILING NOVEL ASPECTS OF D-AMINO ACID METABOLISM IN THE MODEL BACTERIUM PSEUDOMONAS PUTIDA KT2440

D-amino acids (D-AAs) are the α-carbon enantiomers of L-amino acids (L- AAs), the building blocks of proteins in known organisms. It was largely believed that D-AAs are unnatural and must be toxic to most organisms, as they would compete with the L-counterparts for protein synthesis. Recently, new methods have been developed that allow scientists to chromatographically separate the two AA stereoisomers. Since that time, it has been discovered that D-AAs are vital molecules and they have been detected in many organisms. The work of this dissertation focuses on their place in bacterial metabolism. This specific area was selected due to the abundance of D-AAs in bacteria-rich environments and the knowledge of their part in several processes, such as peptidoglycan synthesis, biofilm disassembly, and sporulation. We focused on the bacterium Pseudomonas putida KT2440 which inhabits the densely populated plant rhizosphere. Due to its versatility and cosmopolitan character, this bacterium has provided an excellent system to study D-AA metabolism. In the first chapter, we have developed a new approach to identify specific genes encoding enzymes acting on D-AAs, collectively known as amino acid racemases. Using this novel method, we identified three amino acid racemases encoded by the genome of P. putida KT2440. All of the enzymes were subsequently cloned and purified to homogeneity, followed by a complete biochemical characterization. The aim of the second chapter was to understand the specific role of the peculiar broad-spectrum amino acid racemase Alr identified in chapter one. After constructing a markerless deletion of the cognate gene, we conducted a variety of phenotypic assays that led to a model for a novel catabolic pathway that involves D-ornithine as an intermediate. The work in chapter three identifies for the first time numerous rhizosphere-dwelling bacteria capable of catabolizing D-AAs. Overall, the work in this dissertation contributes a novel understanding of D-AA catabolism in bacteria and aims to stimulate future efforts in this research area

Extending Metal-to-Polyoxometalate Charge Transfer Lifetimes: The Effect of Heterometal Location

In an effort to develop robust molecular sensitizers for solar fuel production, the electronic structure and photodynamics of transition-metal-substituted polyoxometalates (POMs), a novel class of compound in this context, was examined. Experimental and computational techniques including femtosecond (fs) transient absorption spectroscopy have been used to study the cobalt-containing Keggin POMs, [CoIIW12O40]6− (1 a), [CoIIIW12O40]5− (2 a), [SiCoII(H2O)W11O39]6− (3 a), and [SiCoIII(H2O)W11O39]5− (4 a), finding the longest lived charge transfer excited state so far observed in a POM and elucidating the electronic structures and excited-state dynamics of these compounds at an unprecedented level. All species exhibit a bi-exponential decay in which early dynamic processes with time constants in the fs domain yield longer lived excited states which decay with time constants in the ps to ns domain. The initially formed states of 1 a and 3 a are considered to result from metal-to-polyoxometalate charge transfer (MPCT) from CoII to W, while the longer-lived excited state of 1 a is tentatively assigned to a localized intermediate MPCT state. The excited state formed by the tetrahedral cobalt(II) centered heteropolyanion (1 a) is far longer-lived (τ=420 ps in H2O; τ=1700 ps in MeCN) than that of 3 a (τ=1.3 ps), in which the single CoII atom is located in a pseudo-octahedral addendum site. Short-lived states are observed for the two CoIII-containing heteropolyanions 2 a (τ=4.4 ps) and 4 a (τ=6.3 ps) and assigned solely to OCoIII charge transfer. The dramatically extended lifetime for 1 a versus 3 a is ascribed to a structural change permitted by the coordinatively flexible central site, weak orbital overlap of the central Co with the polytungstate framework, and putative transient valence trapping of the excited electron on a single W atom, a phenomenon not noted previously in POMs

Aspects of personal data protection in handling cardiac data

Сърдечната недостатъчност като крайна фаза на всички сърдечни заболявания е проблем, за решаването на който е необходимо лекарят да притежава голям обем информация, свързана със състоянието на пациентите. Процесите на консултации, диагностика и лечение са свързани с обработка на специални категории лични данни, която трябва да бъде приведена в съответствие с общия регламент относно защитата на данните на физическите лица, приет на 4 май 2016г и влизащ в сила от 25 Май 2018г. В доклада се изясняват задължителните изисквания, които е необходимо да бъдат изпълнени, и се дават препоръки за приложимите ор-ганизационни и технически мерки за постигане на съответствие с регламента.Heart failure as an end stage of all heart cardiovascular diseases is a problem for which a physician needs to have a large amount of information related to the condition of patients. Consultation, diagnosis and treatment processes are related to the processing of special categories of personal data, which must be brought into line with the General Data Protection Regulation of Natural Persons adopted on May 4, 2016 and in force since May 25, 2018. The report clarifies the mandatory requirements that need to be met and provides recommendations on the applicable organizational and technical measures to comply with the Regulation

Photoproduction of pi0 omega off protons for E(gamma) < 3 GeV

Differential and total cross-sections for photoproduction of gamma proton to proton pi0 omega and gamma proton to Delta+ omega were determined from measurements of the CB-ELSA experiment, performed at the electron accelerator ELSA in Bonn. The measurements covered the photon energy range from the production threshold up to 3GeV.Comment: 8 pages, 13 figure