Perceptual and Acoustical assessment of voice in children with cleft lip and palate

BACKGROUND: The speech of individuals with cleft lip and palate is primarily characterized by nasality of oral speech because of cleft and or velopharyngeal dysfunction. OBJECTIVES: The aim of the present study is to investigate the acoustical assessment and perceptual rating measurements in cleft lip and palate children. METHODS: The study participants included 30 children with cleft lip and palate in the age range of 4 to 12 years. Prior parental consent was obtained for the inclusion of their children in the study. Speech samples of all the l the participants were recorded. Prerecorded speech samples of the individuals of the CLP groups were mixed and randomized and played,using headphone conditions in a quiet room. The SLPs speech language pathologists rated the nasality of the participant. Samples given for perceptual evaluation are 15 phonation samples of /ă/, /ɪ/ and /ŭ/ vowels vowel, 15 conversation samples and 30 subjects (3 oral sentences each so total 90 sentences). RESULTS: For the Phonation sample, SLP must evaluate the presence of hypernasality by Wilcoksons nasality scale. Acoustic analysis was done using PRAAT software. The spectral and temporal parameters were measured. The Kappa coefficient was 0.88 for inter-rater reliability for nasality rating scale, 0.82 for speech intelligibility,1.02 for speech understandability,1.00 for speech acceptability, which suggests substantial agreement between the raters for four different rating scales. CONCLUSION: Thus, to conclude in children with cleft lip and palate both acoustical and perceptual evaluation plays an important role while assessing the speech domain by using different kinds of speech stimuli

Sestrin 2 as a new biomarker in obesity and diabetes mellitus: A new key player in the metabolic regulation

The global health crisis of diabetes linked to obesity necessitates innovative biomarkers for early diagnosis and targeted treatment. This study explores the pivotal role of sestrins, evolutionarily conserved proteins, in the intricate interplay between obesity and diabetes. Highlighting their significance as regulators of metabolic balance, stress response, and cellular homeostasis, particularly sestrin 2 (SESN2), the study delves into their impact on oxidative stress, insulin sensitivity, and inflammatory responses. Recent studies have highlighted the dysregulation of sestrins in obesity and diabetes mellitus, suggesting their potential as diagnostic markers and therapeutic targets. Examining the molecular processes involving AMP-activated protein kinase (AMPK) and the mechanistic target of rapamycin complex 1 (mTORC1) underscores sestrins as potential biomarkers for prognosis and early detection in obesity and diabetes, offering promising avenues for targeted treatments. This commentary highlights the importance of diagnostic and therapeutic potential of sestrins, thus paving the way for personalized management strategies in obesity and diabetes mellitus

Effect of repeated heating on Chemical Properties of selected edible plant oil and its Health Hazards

Increase in the cost price of edible oil, has forced to reuse the cooking oil especially in frying. Repeated cooking of oil brings about lot of structural changes ideally not suitable for consumption. The present study aims at comparing the structure and chemical changes like acid value, peroxide value, saponification, iodine value of three different commonly used edible oil viz sunflower oil, coconut oil & rice bran oil after successive heating & cooling. Oils were heated four times for 45 minutes, followed by repeated cooling for 2 hours. The results indicates that the acid values of all three oils (0.32- 1.36mg KOH/g)were in line with recommended standard values. The saponification values were gradually increased after every heating in rice bran oil, after the third heating the values crossed the standard limit(252- 278.10mgKOH/g in Coconut oil, 184.23-198.00mgKOH/g in Sunflower oil and 190.81-199.99mgKOH/g in Rice Bran oil.). In coconut and sunflower oil the peroxide values exceeded the standard limit after second heating whereas, in rice bran oil the values exceeded after the first heating implying rancidity releasing free radicals upon heating, peroxide value of Coconut oil (1.00-52.00meq/kg), Sunflower oil (1.00-47.00meq/kg) and Rice Bran oil (10.00-160.00meq/kg), increasing peroxide value over repeated heating indicates higher degree of oxidative rancidity. First heating of all oil showed low acid value indicating stability against peroxidation & rancidity, value increased after repeated heating. Based on the experimental evidence it may be concluded that three commonly used cooking oil, coconut oil, sunflower oil and rice bran oil undergo structural and chemical changes upon repeated heating. However coconut oil and sunflower oil showed structural stability upto second heating, low acid value, saponification ,iodine value and peroxide value were in accordance with reference, where as rice bran oil after first heating showed attributes of oil deterioration with high acid value, saponification, iodine number. Rice bran oil might be good source of other supplemental additives but its repeated heated oil re usage has to be avoided to minimize health risk. Key words: Edible oil, heating, chemical properties, acid value, peroxide value, iodine valu

IN VITRO INHIBITORY ACTIVITY OF MEDICINAL PLANTS AGAINST PHOMOPSIS AZADIRACHTAE, THE INCITANT DIE BACK DISEASE OF NEEM

Objectives: Plant diseases extend a threat to the global production of herbal medicines. Safer plant protection strategies are in need to meet the demands from the pharmaceutical industry. Neem is a tree with great medicinal values, which is being infected by Phomopsis azadirachtae (PA), causing die back disease. The resulting massive destruction of the tree demands optimum disease management methods and thus, the objective of the present study was to evaluate the inhibitory potential of some plant extracts against the growth of PA.Methods: In this study, we investigate the in vitro antifungal activity of ether: chloroform (1:1), ethyl acetate, acetone and ethanol extracts from different parts (leaves and roots) of five plant species viz., Ocimum tenuiflorium, Centella asiatica, Solanum nigrum, Apium graveolens and Besella alba against the growth of PA.Results: All the 40 crude extracts (at concentration of 1000 μg/ml), were active against PA. Among them, the ethanol and ethyl acetate extracts proved optimum with antifungal activity ranging from 65-95% and minimum inhibitory concentration (MIC) values from 75-200 μg/ml. The antifungal activity of the extracts was in the order: ethanol>ethyl acetate>acetone>ether: chloroform (1:1). Also, noteworthy results were observed in all the extracts tested against the spore germination of PA, with concentration and time-dependent kinetics.Conclusion: The results prove promising in order to develop new antifungal agents against the growth and spread of PA.Â

Influence of mastication and its relationship with Body Mass Index before and after prosthetic rehabilitation in partially edentulous patients

BACKGROUND: The main purpose of prosthetic rehabilitation is to enhance the masticatory function by replacing the missing teeth with an artificial substitute, which improves nutrient-rich food intake. There are recent studies which indicate the influence of chewing behavior and energy intake, but little is known about the relationship between chewing on nutritional status. OBJECTIVE: This study intended to assess the changes in masticatory efficiency before and after prosthetic rehabilitation and its influence on nutritional status and body weight. METHODS: A total of 40 partially edentulous subjects aged between 45- 65 years were recruited. Body Mass Index was determined by measuring body weight using a medical grade weighing scale. Height was measured using wall mounted stature meter and Waist circumference was measured with an anthropometric measure tape. Masticatory efficiency was determined using the sieve method with peanuts as test food at baseline, at 3 and 6 months of prosthetic rehabilitation with a removable partial denture. RESULTS: Sieve test performed for evaluating masticatory efficiency showed an increase in the percentage of smaller particles by 28.3% in non-obese and 32.15% in the obese group. The obese/overweight group showed a decrease in BMI values and non-obese subjects showed no significant change in BMI. CONCLUSION: The study concluded that improving masticatory efficiency by prosthodontic rehabilitation can aid in normalizing the nutritional status in certain partially edentulous non-obese and obese individuals

EVALUATION OF BANANA (MUSA SP. VAR. NANJANGUD RASA BALE) FLOWER AND PSEUDOSTEM EXTRACTS ON ANTIMICROBIAL, CYTOTOXICITY AND THROMBOLYTIC ACTIVITIES

Objectives: The present study is centered on potential utilization of banana flower (FB) and pseudostem (PB), as a source of antimicrobial, cytotoxic and thrombolytic contributor, which otherwise is discarded as waste or burnt.Methods: FB and PB, the by-products of banana cultivation were extracted sequentially using various solvents viz., ether: chloroform (1:1), ethyl acetate, acetone, methanol, ethanol and water. In vitro antimicrobial activity of the extracts was tested against six bacterial strains using standard disc diffusion method and the minimal inhibitory concentration (MIC) was performed by microdilution method. Further to validate the safe consumption, extracts were assessed for toxicity evaluation in cell culture against 3T3-L1 cell line (obtained from adipose tissues) using MTT assay.  Also, the thrombolytic activity was performed by clot disruption method.Results: Phytochemical analysis demonstrated that FB and PB were a rich source of polyphenols (saponins, terpenoids, flavonoids, coumarins), cardiac glycosides and steroids. Extracts possessed antimicrobial activities against all the microorganisms tested, with MIC values in the range between 1.2 to 2.5 mg/ml. The investigation on thrombolytic activity by the aqueous extracts of FB (18%) and PB (13%) expressed a significant percentage of clot lysis with reference to Streptokinase (64%). Also, all the extracts of FB and PB exhibited no cytotoxic effect against 3T3L1 cell line.Conclusion: The present work demonstrates the antimicrobial, cytotoxic and thrombolytic activities of FB and PB extracts. The activities exhibited could be the basis for their alleged health promoting abilities and serve as new source of natural nutraceutical with potential applications.Â

Bio prospecting of Lapachol producing endophytic fungi

Background: The association of endophytic fungi with medicinal plants has been one of the evolving areas of research in the past few decades. The secondary metabolites produced owing to such associations have been recognised for a wide range of biological activities. Objectives: The aim of the present review is to highlight the isolation of lapachol from endophytic microorganisms with an emphasis on its biotransformation to improve its efficacy. Methodology: The researchers followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. Published scientific articles on endophytes, host-pathogen interaction and lapachol isolation were collected from reputed journals from 1960 to 2022 using electronic databases using the key words as stated. Following that, the authors chose the required papers based on the criteria they devised. The data was extracted using the common research elements found in the publications. Results and discussion: Lapachol is one such secondary metabolite known for potent antitumor properties. Synthesis of lapachol and its isolation from plant sources have been reported but an expensive process. Therefore, bioprospecting of this metabolite from endophytic fungi have been evaluated by a few researchers. It has been identified that A. niger and Alternaria alternata along with some of the filamentous endophytic fungi have been identified to produce lapachol. Some findings of biotransformation of lapachol to render it more potent have also been reported in the present review. Conclusion: Lapachol is one of the plant secondary metabolites that possess several therapeutic benefits. Owing to its isolation being highly expensive from plant sources, endophytes have been exploited. Furthering to the isolation, the biotrasformation of this bioactive molecule to enhance its efficiency has proven to be useful. In this regard, this review has enlightened some of the biotransformed lapachol and its derivatives with special emphasis on the endophytes that produce them. This review comprehensively highlights the various endophytic sources for lapachol production and its biotransformed derivatives

IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES

Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and β-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneï¬cial role of EF and EE in reducing oxidative stress conditions.Â

THE CURRENT STATUS AND PERSPECTIVES FOR THE EMERGING PANDEMIC: COVID-19

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the extremely communicable viral infection coronavirus disease 19 (covid-19). Initially the virus was found at Wuhan, china which spread across the world exponentially and in a very short span. This outbreak has turned out to be a global health crisis and recently WHO regarded it as pandemic. The origin of the virus is predicted as either the natural selection in animal host prior to the transfer of the pathogen from animals to humans or the natural selection in humans and following transfer. Nevertheless, there is an extensive spread of virus by human to human transfer in the form of droplets. A few antiviral drugs are at the stage of clinical trials to eradicate the covid-19. In this review, a comprehensive approach is put forth to scrutinise the etiology, pathogenicity and transmission of SARS CoV-2. The review also deliberates broadly on the diagnosis and status of therapeutic treatment developed. It also focuses on the preventive and controlling measures from different sectors of the society. The review covers the details reported in 70 studies which were chosen after keyword searches carried out leading to over 884 resulting articles

Competent synthesis of biaryl analogs via asymmetric Suzuki–Miyaura cross-coupling for the development of anti-inflammatory and analgesic agents

Based on the core structure of diflunisal drug, herein, we report a resembling series of biaryl analogs (3a–j) containing halogens, nitro, and methoxy substituents. They were designed and synthesized via a Suzuki–Miyaura cross-coupling reaction using Pd (OH)2 as a catalyst at a temperature of 65 °C with an intent to obtain improved and safer anti-inflammatory and analgesic agents. Suzuki–Miyaura transformation is the most significant among the cross-coupling reactions since its practical advantages include the commercially available low toxic reagents, mild reaction conditions, and functional group compatibility. On the other hand, a few conditions can be used to cross-couple aryl boronic acids or esters with aryl halides, especially 2-benzyl halides. Because of this, a novel Suzuki–Miyaura protocol is investigated that facilitates the selective conversion of halo aromatics, with an emphasis on the reaction to convert substituted bromobenzene to conjugated biphenyls. Finally, the obtained biaryl analogs (3a–j) were tested for in vitro and in vivo anti-inflammatory and analgesic applications. The results showed that compound 3b performed better than the standard drug with IC50 values comparable to that of the standard drug for COX-1 and COX-2 inhibition. Finally, molecular docking tests for the effective compound were carried out