Determination of protective properties of Bardejovske Kuple spa curative waters by rotational viscometry and ABTS assay

Mineral waters from Bardejovske Kupele spa are natural, strongly mineralized, with healing effects. They are classified as hydrocarbonic - containing chloride and sodium - carbonic, cold, hypotonic, with a relatively high boric acid content. Potential anti-oxidative effects of curative waters from Bardejovske Kupele were investigated against the hyaluronan (HA) degradation. High-molar-mass HA was exposed to the action of ascorbate and cupric ions, which initiate free-radical chain degradation. Time-dependent changes of dynamic viscosity (h) of the HA solutions were monitored by rotational viscometry. The radical scavenging capacity of curative waters was determined by the ABTS assay. Despite a significantly high content of transition metal ions, especially iron, remarkable protective effects of the two curative spa waters were found, namely Alzbeta and Klara. Even though “Alzbeta´s“ iron content was 3.5-fold higher than “Klara´s“, “Alzbeta“ was shown to have better protective properties against the HA degradation compared to “Klara“. Bolus addition of ferric ions to the reaction system instead of the natural iron-containing curative water caused a significant HA degradation. The ABTS decolorization assay revealed that the curative spa waters were proven as poorly effective scavengers of the ABTS·+ cation radical

New multiresponsive chromic soft materials: dynamic interconversion of short 2,7-dicyanomethylenecarbazole-based biradicaloid and the corresponding cyclophane tetramer

This work reports on a quinodimethane-type molecule, 2,7-dicyanomethylene-9-(2-ethylhexyl)carbazole (1), one of the shortest π-conjugated biradicaloids reported to be stable in solution under ambient conditions. This carbazole-based quinoidal precursor is able to form a macrocyclic σ-bonded tetramer (2). The resolved single-crystal X-ray structure of tetramer 2 shows that four molecules of 1 are linked together through four long (CN)2C−C(CN)2 bonds (1.631 Å) resulting from coupling of the unpaired electrons in biradicaloid 1. Dynamic interconversion between monomer 1 and cyclophane tetramer 2 is achieved by reversible cleavage and recovery of the four (CN)2C−C(CN)2 bonds upon soft external stimuli (light absorption, temperature and pressure), which is accompanied by significant color changes. These novel photo-, thermo-, and mechanochromic properties expand the versatility of π-conjugated biradicaloid compounds as novel functional materials that, in combination with spin chemistry and dynamic covalent chemistry, can be relevant in molecular machines, sensors, and switches

Cultivation of carotenogenic yeasts on waste substrates usinf solid state fermentation

„Semi-solid fermentation“ je čoraz viac využívanou technikou k produkcii významných metabolitov, či obohatenej biomasy za nízkych finančných nákladov, nízkej spotreby kultivačnej vody a zároveň s malou záťažou na životné prostredie. Problémom tejto techniky ale môže byť voľba vhodného mikroorganizmu, ktorý je schopný rásť a produkovať pri nízkej aktivite vody. Predložená práca je poňatá ako pilotná štúdia troch kvasinkových kmeňov Rhodotorula glutinis, Cystofilobasidium capitatum a Sporobomomyces roseus kultivovaných technikou „semi-solid state fermentation“ (semi-SSF). Kvasinky boli kultivované v sériách produkčných médií s postupným znižovaním obsahu kultivačnej vody. Ako uhlíkatý zdroj sacharidov boli použité cestoviny a hydrolyzované cestoviny, v kontrolných médiách glukóza. Všetky študované kmene boli schopné rásť a produkovať sledované lipidické metabolity i pri nízkych obsahoch vody. Produkcie karotenoidov a sterolových látok boli vyššie práve v médiách s nízkou aktivitou vody. Bunky sa tak pravdepodobne chránili pred väčším osmotickým tlakom. Ako najlepší producent karotenoidov i biomasy sa ukázal kmeň Cystofilobasidium capitatum, kultivovaný na médiu s hydrolyzovanými cestovinami a obsahom vody 40%. Semi-SSF sa javí ako dobrá technika pre selekciu kmeňov s nadprodukčnými vlastnosťami. Výberom vhodného produkčného média a koncentrácie vody sa dá optimalizovať stimulácia produkcie sledovaných metabolitov v kvasinkových bunkách.Semi-solid fermentation is an eco - friendly technique more and more used for production of significant metabolites or enriched biomass at low entrance cost and low comsumption of water. The problem of this technique might be the right choice of microorganism able to grow and produce at low water activity. This work is a pilot study of three red yeast strains – Rhodotorula glutinis, Cystofilobasidium capitatum and Sporobomomyces roseus cultivated by semi-solid state fermentation (semi-SSF). Yeasts were cultivated in series of production media with gradual reduction of cultivation water content. Pasta and hydrolyzed pasta were used as source of sacharides, glucose served as the carbon source in control media. All studied strains we able to grow and produce observed lipidic metabolites also at low water contents. Production of carotenoids and sterols was higher in semi-solid media. Cystofilobasidium capitatum strain was identified as the best producer of carotenoids and biomass. This strain was cultivated on hydrolyzed pasta media with 40% water content. Semi-SSF seems to be an adequate technique for selection of strains having over-productive properties. Observed metabolites production in yeast cells can be optimized by choosing the appropriate production media and water activity.

Implemntasi Kebijakan Keputusan Gubernur No 981 Tahun 2022 Tentang Tim Percepatan Penurunan Stunting di Kelurahan Cawang Tahun 2022-2023

Penelitian ini berfokus pada Implementasi Kebijakan Keputusan Gubernur No 981 Tahun 2022 Tentang Tim Percepatan Penurunan Stunting Di Kelurahan Cawang Tahun 2022-2023. Tujuan penelitian ini adalah untuk mengetahui kebijakan stunting dan dapat memberikan rekomendasi kebijakan yang relavan untuk meningkatkan efektivitas program pencegahan dan penanganan stunting di Kelurahan Cawang. Salah satu metode yang digunakan adalah pendekatan penelitian kualitatif. Desain penelitian menggunakan deskriptif analitis dan teknik pengumpulan data wawancara dan dokumentasi. Teori yang diterapkan adalah teori implementasi kebijakan George Edward III. Penelitian ini menunjukkan bahwa upaya pencegahan dan penanganan stunting telah dilakukan di Kelurahan Cawang. Salah satunya kebijakan yang telah dilakukan adalah Pemberiaan Makanan Tambahan (PMT). Setelah upaya intervensi yang di lakukan, jumlah anak dari 29 stunting balita berhasil turun menjadi 18 anak. Hal ini menunjukkan adanya kemajuan yang signifikan dalam upaya penurunan stunting dan peningkatan status gizi anak-anak di Kelurahan Cawang. Kata kunci: Kebijakan, Stunting, Pencegahan, Penanganan / This research focuses on the implementation of Governor's Decree No. 981 of 2022 concerning the Acceleration Team for Stunting Reduction in Cawang Subdistrict in 2022-2023. The aim of this research is to understand stunting policies and provide relevant policy recommendations to increase the effectiveness of stunting prevention and management programs in Cawang Village. One of the methods used is a qualitative research approach. The research design uses analytical descriptive and interview and documentation data collection techniques. The theory applied is George Edward III's theory of policy im plementation. This research shows that efforts to prevent and handle stunting have been carried out in Cawang Village. One of the policies that has been implemented is the provision of supplementary food (PMT). After intervention efforts were carried out, the number of children from 29 stunted toddlers was reduced to 18 children. This shows significant progress in efforts to reduce stunting and improve the nutritional status of children in Cawang Village. Keywords: Policy, Stunting, Prevention, Treatmen

Exploring redox states, doping and ordering of electroactive star-shaped oligo(aniline)s

We have prepared a simple star‐shaped oligo(aniline) (TDPB) and characterised it in detail by MALDI‐TOF MS, UV/Vis/NIR spectroscopy, time‐dependent DFT, cyclic voltammetry and EPR spectroscopy. TDPB is part of an underdeveloped class of π‐conjugated molecules with great potential for organic electronics, display and sensor applications. It is redox active and reacts with acids to form radical cations. Acid‐doped TDPB shows behaviour similar to discotic liquid crystals, with X‐ray scattering investigations revealing columnar self‐assembled arrays. The combination of unpaired electrons and supramolecular stacking suggests that star‐shaped oligo(aniline)s like TDPB have the potential to form conducting nanowires and organic magnetic materials

Are the metal identity and stoichiometry of metal complexes important for colchicine site binding and inhibition of tubulin polymerization?

Quite recently we discovered that copper(II) complexes with isomeric morpholine-thiosemicarbazone (morph-TSCs) hybrid ligands show good cytotoxicity in cancer cells and that the molecular target responsible for this activity might be..

Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

Carotenogenic yeasts are non-conventional oleaginous microorganisms capable to utilize various waste substrates. In this work 4 red yeast strains (Rhodotorula, Cystofilobasidium and Sporobolomyces sp.) were cultivated in media containing crude, emulsified and enzymatically hydrolysed animal waste fat, compared with glucose and glycerol as single C-sources. Cell morphology (cryo-SEM, TEM), production of biomass, lipase, biosurfactants, lipids (GC/FID) carotenoids, ubiquinone, ergosterol (HPLC/PDA) in yeast cells was studied depending on medium composition, C-source and C/N ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N=13 was higher on emulsified/hydrolysed fat than on glucose/glycerol. Production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/l) and lipids (54.5%/CDW) were found in S.pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. Increased C/N ratio (13-100) led to higher biomass production in fat media. Production of total lipids increased in all strains to C/N 50. Oppositely, production of carotenoids, ubiquinone and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts are having great application potential and can result from valorization of animal waste fat in the biorefinery concept.Karotenogenní kvasinky jsou schopny utilizovat řadu různých substrátů včetně odpadů. V této práci je popsána kultivace vybraných kmenů karotenogenních kvasinek rodů Rhodotorula, Cystofilobasidium, and Sporobolomyces sp. v médiu obsahujícícm surový odpadní živočišný tuk a upravený substrát emulsifikací a enzymovou hydrolýzou. Výsledky byly srovnány s kultivací na glukóze a glycerolu jako jednoduchých zdrojích uhlíku

Physical properties and cytotoxicity of Cu(ii) and Zn(ii) complexes with a TMS-substituted indolo[2,3-c]quinoline-derived Schiff base

The incorporation of non-native chemical elements, such as silicon, into drug molecules has gained significant attention as a strategy to broaden the chemical space in medicinal chemistry and develop novel drug candidates. Traditionally, research has focused on the isosteric replacement of a carbon atom with silicon ("silicon switch") in known drug structures or the attachment of a trimethylsilyl (TMS) group to biologically active scaffolds. In this study, a TMS-substituted indoloquinoline-based Schiff base (HLTMS) and its corresponding metal complexes, Cu(HLTMS)Cl2 (1) and Zn(HLTMS)Cl2 (2), were synthesized and comprehensively characterized using elemental analysis, spectroscopic techniques (IR, UV-vis, 1H and 13C NMR for HLTMS and 2), ESI mass spectrometry and single-crystal X-ray diffraction (SC-XRD) for 1 and electron diffraction (ED) for 2. The attachment of the TMS group enhanced the lipophilicity of HLTMS, while complex formation with Cu(ii) substantially improved the antiproliferative activity. Exploitation of their intrinsic fluorescence to investigate cellular uptake and intracellular localization in cancer cells was impeded by limited solubility. Both HLTMS and 2 were found to generate reactive oxygen species under cell-free conditions in accord with their redox activity established by cyclic voltammetry. The photochemical activity of the indolo[2,3-c]quinoline-based proligand HLTMS and its complexes 1 and 2 has been disclosed. The compounds exhibited significant toxicity on various human cancer cells and disrupted the mitochondrial membrane potential, suggesting the contribution of mitochondrial dysfunction, triggered by HLTMS and its metal complexes, to their toxic effects. These findings highlight the potential of TMS-substituted Schiff bases as promising anticancer drug candidates