Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Comparatıve analysıs of mıcronutrıent transporter proteıns ın some monocot and dıcot plant specıes by usıng bıoınformatıcs tools

ÖZETBAZI MONOKOTİL VE DİKOTİL BİTKİLERDE MİKROBESİN ELEMENTİ TAŞIYICI PROTEİNLERİN BİYOİNFORMATİK ARAÇLARLA KARŞILAŞTIRMALI ANALİZLERİMikrobesin elementleri bitkilerde birincil ve ikincil metabolizmada çok önemli roller oynamaktadır. Bununla birlikte, bu besin elementlerinin eksikliği ya da fazla miktarları çeşitli semptomlara yol açarak bitkilerin hayat döngüsünü olumsuz bir şekilde etkilemektedir. Bu yüzden bitkiler mikro besin elementlerinin taşınmasında hassas bir mekanizmaya ihtiyaç duyarlar. Bu çalışmamız, seçilmiş 18 bitki türünde çeşitli biyoinformatik araçları ve yaklaşımları kullanarak bitki köklerinden demir (Fe), çinko (Zn), bor (B), bakır (Cu), molibden (Mo), silicon (Si) ve manganez (Mn) gibi mikrobesin elementlerinin alınımından sorumlu taşıyıcı proteinlerin belirlenlenmesini ve karakterize edilmesini amaçlamaktadır. Çalışmamızda kullanılan bitki türleri şunlardır; Arabidopsis thaliana, Brachypodium distachyon, Brassica rapa, Chlamydomonas reinhardtii (yeşil alg), Cucumis sativus, Eucalyptus grandis, Glycine max, Gossypium raimondii, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens (yosun), Populus trichocarpa, Prunus persica, Solanum lycopersicum, Sorghum bicolor, Vitis vinifera ve Zea mays. Bu çerçevede, mikrobesin elementlerinin taşınmasından sorumlu potansiyel genler ve proteinler homoloji benzerliği kullanılarak bu türlerin proteom veri setlerinden belirlendi; fiziko-kimyasal özellikleri, korunmuş motif dizileri ve ekzon/intron organizasyonları analiz edildi; hücre-altı lokalizasyonları ve transmembrane domain (TMD) topolojileri belirlendi; taşıyıcı proteinlerin sekansları kullanılarak, karşılaştırmalı filogenetik ağaçları ve protein-protein ilişkileri kuruldu; ayrıca taşıyıcı proteinlerin 3D yapıları modellendi. Farklı bitki türlerinde mikrobesin elementlerinin alınımından sorumlu gen ve proteinlerin belirlenip karakterize edilmesi gelecekteki çalışmalar için çok değerli bir bilgi kaynağı olacaktır, aynı zamanda bu çalışma bitkilerde ürün kalitesinin ve miktarının iyileştirilmesi açısından mikrobesin elementi taşıyıcılarının manipülasyonu çalışmaları için de bir fikir oluşturacaktır.ABSTRACTCOMPARATIVE ANALYSIS OF MICRONUTRIENT TRANSPORTER PROTEINS IN SOME MONOCOT AND DICOT PLANT SPECIES BY USING BIOINFORMATICS TOOLS Micronutrients play very crucial roles in primary and secondary metabolisms in plants. However, deficiency or toxicity of these micronutrients could adversely affect the plant life cycle by causing a variety of symptoms. Therefore, plants need an elaborate mechanism of micronutrient transport system. This study aimed to identify and characterize the main root uptake transporters of iron (Fe), zinc (Zn), boron (B), copper (Cu), molybdenum (Mo), silicon (Si) and manganese (Mn) in 18 selected plant species by using various bioinformatics tools and approaches. These species included Arabidopsis thaliana, Brachypodium distachyon, Brassica rapa, Chlamydomonas reinhardtii (green alga), Cucumis sativus, Eucalyptus grandis, Glycine max, Gossypium raimondii, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens (moss), Populus trichocarpa, Prunus persica, Solanum lycopersicum, Sorghum bicolor, Vitis vinifera and Zea mays. In this context, potential micronutrient transporter genes and proteins in 18 plants were identified from the proteome datasets of these species using homology search; physicochemical properties of these transporters were determined; conserved motif sequences and exon/intron organization were analyzed; sub-cellular localization and transmembrane domain (TMD) topologies were predicted; using protein sequences, comparative phylogenetic trees and interactome map were constructed, and 3D models of these transporters were generated. Identification and characterization of micronutrient transporter genes and proteins in various plant species will become valuable theoretical knowledge for future studies, and will provide an insight for studies of manipulation of micronutrient transporters in terms of improving crop quality and quantity

Comparatıve analysıs of mıcronutrıent transporter proteıns ın some monocot and dıcot plant specıes by usıng bıoınformatıcs tools

BAZI MONOKOTİL VE DİKOTİL BİTKİLERDE MİKROBESİN ELEMENTİ TAŞIYICI PROTEİNLERİN BİYOİNFORMATİK ARAÇLARLA KARŞILAŞTIRMALI ANALİZLERİ Mikrobesin elementleri bitkilerde birincil ve ikincil metabolizmada çok önemli roller oynamaktadır. Bununla birlikte, bu besin elementlerinin eksikliği ya da fazla miktarları çeşitli semptomlara yol açarak bitkilerin hayat döngüsünü olumsuz bir şekilde etkilemektedir. Bu yüzden bitkiler mikro besin elementlerinin taşınmasında hassas bir mekanizmaya ihtiyaç duyarlar. Bu çalışmamız, seçilmiş 18 bitki türünde çeşitli biyoinformatik araçları ve yaklaşımları kullanarak bitki köklerinden demir (Fe), çinko (Zn), bor (B), bakır (Cu), molibden (Mo), silicon (Si) ve manganez (Mn) gibi mikrobesin elementlerinin alınımından sorumlu taşıyıcı proteinlerin belirlenlenmesini ve karakterize edilmesini amaçlamaktadır. Çalışmamızda kullanılan bitki türleri şunlardır; Arabidopsis thaliana, Brachypodium distachyon, Brassica rapa, Chlamydomonas reinhardtii (yeşil alg), Cucumis sativus, Eucalyptus grandis, Glycine max, Gossypium raimondii, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens (yosun), Populus trichocarpa, Prunus persica, Solanum lycopersicum, Sorghum bicolor, Vitis vinifera ve Zea mays. Bu çerçevede, mikrobesin elementlerinin taşınmasından sorumlu potansiyel genler ve proteinler homoloji benzerliği kullanılarak bu türlerin proteom veri setlerinden belirlendi; fiziko-kimyasal özellikleri, korunmuş motif dizileri ve ekzon/intron organizasyonları analiz edildi; hücre-altı lokalizasyonları ve transmembrane domain (TMD) topolojileri belirlendi; taşıyıcı proteinlerin sekansları kullanılarak, karşılaştırmalı filogenetik ağaçları ve protein-protein ilişkileri kuruldu; ayrıca taşıyıcı proteinlerin 3D yapıları modellendi. Farklı bitki türlerinde mikrobesin elementlerinin alınımından sorumlu gen ve proteinlerin belirlenip karakterize edilmesi gelecekteki çalışmalar için çok değerli bir bilgi kaynağı olacaktır, aynı zamanda bu çalışma bitkilerde ürün kalitesinin ve miktarının iyileştirilmesi açısından mikrobesin elementi taşıyıcılarının manipülasyonu çalışmaları için de bir fikir oluşturacaktır. ABSTRACT COMPARATIVE ANALYSIS OF MICRONUTRIENT TRANSPORTER PROTEINS IN SOME MONOCOT AND DICOT PLANT SPECIES BY USING BIOINFORMATICS TOOLS Micronutrients play very crucial roles in primary and secondary metabolisms in plants. However, deficiency or toxicity of these micronutrients could adversely affect the plant life cycle by causing a variety of symptoms. Therefore, plants need an elaborate mechanism of micronutrient transport system. This study aimed to identify and characterize the main root uptake transporters of iron (Fe), zinc (Zn), boron (B), copper (Cu), molybdenum (Mo), silicon (Si) and manganese (Mn) in 18 selected plant species by using various bioinformatics tools and approaches. These species included Arabidopsis thaliana, Brachypodium distachyon, Brassica rapa, Chlamydomonas reinhardtii (green alga), Cucumis sativus, Eucalyptus grandis, Glycine max, Gossypium raimondii, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens (moss), Populus trichocarpa, Prunus persica, Solanum lycopersicum, Sorghum bicolor, Vitis vinifera and Zea mays. In this context, potential micronutrient transporter genes and proteins in 18 plants were identified from the proteome datasets of these species using homology search; physicochemical properties of these transporters were determined; conserved motif sequences and exon/intron organization were analyzed; sub-cellular localization and transmembrane domain (TMD) topologies were predicted; using protein sequences, comparative phylogenetic trees and interactome map were constructed, and 3D models of these transporters were generated. Identification and characterization of micronutrient transporter genes and proteins in various plant species will become valuable theoretical knowledge for future studies, and will provide an insight for studies of manipulation of micronutrient transporters in terms of improving crop quality and quantity

Genome-wide identification of mildew resistance locus O (MLO) genes in tree model poplar (Populus trichocarpa): powdery mildew management in woody plants

Filiz, Ertugrul/0000-0001-9636-6389WOS: 000440108100008Poplars are economically important fast growing trees. They are exposed to broad range of fungal diseases like powdery mildew (PM). MLOs (mildew resistance locus O), as plant susceptibility genes, act as negative regulators and whose loss-of-functions confer complete resistance to PM disease. Herein, work identified the MLO gene family members in poplar, a woody model species. A total of 26 identified MLOs (annotated as PtMLO1-26) were distributed on 14 poplar chromosomes either individually or in groups of two to four. PtMLO genes encoded a polypeptide of 341-593 residues with a characteristic MLO domain structure. One tandem and eight segmental duplications were revealed in PtMLO genes. PtMLO proteins anchored at plasma membrane and had putative 5-9 TMDs with extracellular/cytosolic N- and C-terminuses. They were rich in leucine (9.1-12.9%), which is reported to play roles in defense response signaling. The C-terminal calmodulin-binding domain (CaMBD), reported to modulate the signaling mechanism in the defense response, was completely preserved in all PtMLOs, except PtMLO6. This domain was partially absent in PtMLO6 which is inferred to be a different MLO type or a pseudogene with a lost/impaired function in PM response. Besides, second and third cytoplasmic loops that are critical for PM-susceptibility were identified in PtMLOs. Particularly, PtMLO17, 18, 19, and 24 genes, inferred from Arabidopsis-poplar comparative phylogeny, were identified as potential candidates that may be involved in poplar-PM resistance. Notably, inductions of 14 PtMLO genes were detected in probes of microarray data such as GSE56865, GSE16417, and GSE23726 under different fungal infections indicating their involvements in plant defense. Overall, this work provided a basis for woody plant genomics for the effective and better management of poplar-PM disease