A systematic review of variables used to assess clinically acceptable alignment of unilateral transtibial amputees in the literature.

Prosthetic alignment is a subjective concept which lacks reliability. The outcome responsiveness to prosthetic alignment quality could help to improve subjective and instrument assisted prosthetic alignment. This study was aimed to review variables used to assess clinically acceptable alignment in the literature. The search was done in some databases including: Google Scholar, PubMed, EBSCO, EMBASE, ISI Web of Knowledge and Scopus. The first selection criterion was based on abstracts and titles to address the research questions of interest. The American Academy of Orthotics and Prosthetics checklists were used for paper risk of bias assessment. A total of 25 studies were included in this study. Twenty-four studies revealed the critics of standing position or walking to locate clinically acceptable alignment, only one study measured outcomes in both situations. A total of 253 adults with transtibial amputations and mean age of 48.71 years participated in included studies. The confidence level of included studies was low to moderate, and before-after trial was the most common study design (n = 19). The joint angle, load line location with respect to joints and center of pressure-related parameters were reported as sensitive outcomes to prosthetic alignment quality in standing posture. The amount of forces at various parts of gait cycle and time of events were sensitive to prosthetic alignment quality during walking. Standing balance and posture and temporal parameters of walking could help to locate clinically acceptable alignment.N/

Pengaruh Organizational Commitment Terhadap Organizational Citizenship Behavior (OCB) Petugas di Lembaga Pemasyarakatan Kelas I Medan

Anggota organisasi perlu memiliki perilaku kewarganegaraan organisasi atau Organizational Citizenship Behavior (OCB) bagi organisasi, begitu juga dengan Petugas di Lembaga Pemasyarakatan Kelas I Medan guna membantu pelaksanaan operasional organisasi. Terdapat banyak faktor yang mempengaruhi Organizational Citizenship Behavior, salah satunya adalah Organizational Commitment. Penelitian ini dilaksanakan dengan tujuan untuk mengetahui pengaruh Organizational Commitment terhadap Organizational Citizenship Behavior (OCB) Petugas di Lembaga Pemasyarakatan Kelas I Medan. Metode penelitian yang digunakan yaitu metode penelitian kuantitatif   kepada 139 petugas di Lembaga Pemasyarakatan Kelas I Medan. Hasil penelitian menunjukkan terdapat pengaruh yang diberikan variabel bebas terhadap variabel terikat dalam penelitian yang didapatkan dari hasil uji t untuk menguji hipotesis. Peneliti mendapatkan nilai t hitung (4,609) > t tabel (1,97718) sehingga H0 ditolak dan Ha diterima, dimana menandakan bahwa terdapat pengaruh Organizational Commitment terhadap Organizational Citizenship Behavior. Peneliti kemudian melakukan uji determinasi dan memperoleh nilai R square sebesar 0,134 yang mana menunjukkan bahwa besar pengaruh work engagement sebagai variabel independen terhadap kinerja pegawai sebagai variabel dependen sebesar 13,4% sedangkan 86,6% sisanya dipengaruhi oleh variabel lain yang tidak dijelaskan dalam penelitian ini. Kesimpulan dari penelitian ini adalah terdapat hubungan yang positif dan signifikan antara Organizational Commitment dengan Organizational Citizenship Behavior di Lembaga Pemasyarakatan Kelas I Medan

Characterizing genomic alterations in cancer by complementary functional associations.

Systematic efforts to sequence the cancer genome have identified large numbers of mutations and copy number alterations in human cancers. However, elucidating the functional consequences of these variants, and their interactions to drive or maintain oncogenic states, remains a challenge in cancer research. We developed REVEALER, a computational method that identifies combinations of mutually exclusive genomic alterations correlated with functional phenotypes, such as the activation or gene dependency of oncogenic pathways or sensitivity to a drug treatment. We used REVEALER to uncover complementary genomic alterations associated with the transcriptional activation of β-catenin and NRF2, MEK-inhibitor sensitivity, and KRAS dependency. REVEALER successfully identified both known and new associations, demonstrating the power of combining functional profiles with extensive characterization of genomic alterations in cancer genomes

The effect of midazolam on end-tidal concentration of isoflurane necessary to prevent movements in dogs

Objective To determine the possible additive effect of midazolam, a GABAA agonist, on the end-tidal concentration of isoflurane that prevents movement (MACNM) in response to noxious stimulation. Study design Randomized crossover experimental study. Animals Six, healthy, adult intact male, mixed-breed dogs. Methods After baseline isoflurane MACNM (MACNM-B) determination, midazolam was administered as a low (LDS), medium (MDS) or high (HDS) dose series of midazolam. Each series consisted of two dose levels, low and high. The LDS was a loading dose (Ld) of 0.2 mg kg-1 and CRI (2.5 μg kg-1 minute-1) (LDL), followed by an Ld (0.4 mg kg-1) and CRI (5 μg kg-1 minute-1) (LDH). The MDS was an Ld (0.8 mg kg-1) and CRI (10 μg kg-1 minute-1) (MDL) followed by an Ld (1.6 mg kg-1) and CRI (20 μg kg-1 minute-1) (MDH). The HDS was an Ld (3.2 mg kg-1) and CRI (40 μg kg-1 minute-1) (HDL) followed by an Ld (6.4 mg kg-1) and CRI (80 μg kg-1 minute-1) (HDH). MACNM was re-determined after each dose in each series (MACNM-T). Results The median MACNM-B was 1.42. MACNM-B did not differ among groups (p \u3e 0.05). Percentage reduction in MACNM was significantly less in the LDS (11 ± 5%) compared with MDS (30 ± 5%) and HDS (32 ± 5%). There was a weak correlation between the plasma midazolam concentration and percentage MACNM reduction (r = 0.36). Conclusion and clinical relevance Midazolam doses in the range of 10-80 μg kg-1 minute-1 significantly reduced the isoflurane MACNM. However, doses greater than 10 μg kg-1 minute-1 did not further decrease MACNM indicating a ceiling effect

Anesthesia of the geriatric equine

Advancements in veterinary medicine have resulted in an increased number of geriatric horses being presented for medical or surgical procedures that require general anesthesia. Due to the physiological changes associated with aging and the likelihood of concurrent disease conditions, the geriatric equine is at an increased risk during anesthesia. The main physiological changes associated with aging, and their impact on anesthesia, are discussed in this review

The Effect of Use of Text Messaging on Soccer Fans’ Behavior (The Case of Iranian Professional League-Persian Gulf League)

The purpose of study is to identify the effect of mobile marketing on the Top Iranian Football Clubs fans’ behavior in 2019-2020 season. The statistical population are the spectators who had signed up in the fan online system and had watched one of games in the stadium. As an accurate number of the statistical population could not be determined, a statistical population of 396 was considered based on the Morgan’s table. In this study, the author collects data on the behavioral loyalty, attitudinal loyalty, and mobile marketing using survey. The cross-sectional regression estimation used to analyze the data. The findings of this study indicate that there is a positive and meaningful relationship between mobile marketing and fans’ behavioral loyalty in Iranian soccer Pro league. Also, there is a positive and meaningful correlation between mobile marketing and the attitudinal loyalty of the soccer Club fans. This study has implications for managers and sponsors of soccer clubs

Identification and ranking of the marketing mix of services in the banking industry, using DEMATEL

In the current competitive market, identifying the major factors affecting the behavior of customers considered very important. Every organization needs to know, investing on which factor results in higher efficiency. Banks face a complex world of competition thus; they need to identify these factors. These factors differ from the factors in manufacturing industry. Therefore, banks need to understand their own marketing mix factors and identify the most important ones. As a result, in this research after a literature review, the factors identified and then by using DEMATEL, a ranking has done and the most important factor introduced. According to the results, price and staff have the most impact

Current strategies for treatment of intervertebral disc degeneration: substitution and regeneration possibilities

Background: Intervertebral disc degeneration has an annual worldwide socioeconomic impact masked as low back pain of over 70 billion euros. This disease has a high prevalence over the working age class, which raises the socioeconomic impact over the years. Acute physical trauma or prolonged intervertebral disc mistreatment triggers a biochemical negative tendency of catabolic-anabolic balance that progress to a chronic degeneration disease. Current biomedical treatments are not only ineffective in the long-run, but can also cause degeneration to spread to adjacent intervertebral discs. Regenerative strategies are desperately needed in the clinics, such as: minimal invasive nucleus pulposus or annulus fibrosus treatments, total disc replacement, and cartilaginous endplates decalcification. Main Body: Herein, it is reviewed the state-of-the-art of intervertebral disc regeneration strategies from the perspective of cells, scaffolds, or constructs, including both popular and unique tissue engineering approaches. The premises for cell type and origin selection or even absence of cells is being explored. Choice of several raw materials and scaffold fabrication methods are evaluated. Extensive studies have been developed for fully regeneration of the annulus fibrosus and nucleus pulposus, together or separately, with a long set of different rationales already reported. Recent works show promising biomaterials and processing methods applied to intervertebral disc substitutive or regenerative strategies. Facing the abundance of studies presented in the literature aiming intervertebral disc regeneration it is interesting to observe how cartilaginous endplates have been extensively neglected, being this a major source of nutrients and water supply for the whole disc. Conclusion: Severalinnovative avenues for tackling intervertebral disc degeneration are being reported â from acellular to cellular approaches, but the cartilaginous endplates regeneration strategies remain unaddressed. Interestingly, patient-specific approaches show great promise in respecting patient anatomy and thus allow quicker translation to the clinics in the near future.The authors would like to acknowledge the support provided by the Portuguese Foundation for Science and Technology (FCT) through the project EPIDisc (UTAP-EXPL/BBBECT/0050/2014), funded in the Framework of the “International Collaboratory for Emerging Technologies, CoLab”, UT Austin|Portugal Program. The FCT distinctions attributed to J. Miguel Oliveira (IF/00423/2012 and IF/01285/ 2015) and J. Silva-Correia (IF/00115/2015) under the Investigator FCT program are also greatly acknowledged.info:eu-repo/semantics/publishedVersio

Growth arrest-specific transcript 5 associated snoRNA levels are related to p53 expression and DNA damage in colorectal cancer

BACKGROUND The growth arrest-specific transcript 5 gene (GAS5) encodes a long noncoding RNA (lncRNA) and hosts a number of small nucleolar RNAs (snoRNAs) that have recently been implicated in multiple cellular processes and cancer. Here, we investigate the relationship between DNA damage, p53, and the GAS5 snoRNAs to gain further insight into the potential role of this locus in cell survival and oncogenesis both in vivo and in vitro. METHODS We used quantitative techniques to analyse the effect of DNA damage on GAS5 snoRNA expression and to assess the relationship between p53 and the GAS5 snoRNAs in cancer cell lines and in normal, pre-malignant, and malignant human colorectal tissue and used biological techniques to suggest potential roles for these snoRNAs in the DNA damage response. RESULTS GAS5-derived snoRNA expression was induced by DNA damage in a p53-dependent manner in colorectal cancer cell lines and their levels were not affected by DICER. Furthermore, p53 levels strongly correlated with GAS5-derived snoRNA expression in colorectal tissue. CONCLUSIONS In aggregate, these data suggest that the GAS5-derived snoRNAs are under control of p53 and that they have an important role in mediating the p53 response to DNA damage, which may not relate to their function in the ribosome. We suggest that these snoRNAs are not processed by DICER to form smaller snoRNA-derived RNAs with microRNA (miRNA)-like functions, but their precise role requires further evaluation. Furthermore, since GAS5 host snoRNAs are often used as endogenous controls in qPCR quantifications we show that their use as housekeeping genes in DNA damage experiments can lead to inaccurate results