Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment

Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. This study investigated marine bacterial communities involved in the metabolism of C1 compounds. These communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. In addition to using 16S rRNA gene fingerprinting and clone libraries to characterize samples taken from a bloom transect in July 2006, seawater samples from the phytoplankton bloom were incubated with 13C-labeled methanol, monomethylamine, dimethylamine, methyl bromide, and dimethyl sulfide to identify microbial populations involved in the turnover of C1 compounds, using DNA stable isotope probing. The [13C]DNA samples from a single time point were characterized and compared using denaturing gradient gel electrophoresis (DGGE), fingerprint cluster analysis, and 16S rRNA gene clone library analysis. Bacterial community DGGE fingerprints from 13C-labeled DNA were distinct from those obtained with the DNA of the nonlabeled community DNA and suggested some overlap in substrate utilization between active methylotroph populations growing on different C1 substrates. Active methylotrophs were affiliated with Methylophaga spp. and several clades of undescribed Gammaproteobacteria that utilized methanol, methylamines (both monomethylamine and dimethylamine), and dimethyl sulfide. rRNA gene sequences corresponding to populations assimilating 13C-labeled methyl bromide and other substrates were associated with members of the Alphaproteobacteria (e.g., the family Rhodobacteraceae), the Cytophaga-Flexibacter-Bacteroides group, and unknown taxa. This study expands the known diversity of marine methylotrophs in surface seawater and provides a comprehensive data set for focused cultivation and metagenomic analyses in the future

Placental Transfusion for Asphyxiated Infants.

The current recommendation for umbilical cord management of non-vigorous infants (limp, pale, and not breathing) who need resuscitation at birth is to immediately clamp the umbilical cord. This recommendation is due in part to insufficient evidence for delayed cord clamping (DCC) or umbilical cord milking (UCM). These methods may provide a neuroprotective mechanism that also facilitates cardiovascular transition for non-vigorous infants at birth

Kinematic classifications of local interacting galaxies: implications for the merger/disk classifications at high-z

The classification of galaxy mergers and isolated disks is key for understanding the relative importance of galaxy interactions and secular evolution during the assembly of galaxies. The kinematic properties of galaxies as traced by emission lines have been used to suggest the existence of a significant population of high-z star-forming galaxies consistent with isolated rotating disks. However, recent studies have cautioned that post-coalescence mergers may also display disk-like kinematics. To further investigate the robustness of merger/disk classifications based on kinematic properties, we carry out a systematic classification of 24 local (U)LIRGs spanning a range of galaxy morphologies: from isolated spiral galaxies, ongoing interacting systems, to fully merged remnants. We artificially redshift the WiFeS observations of these local (U)LIRGs to z=1.5 to make a realistic comparison with observations at high-z, and also to ensure that all galaxies have the same spatial sampling of ~900 pc. Using both kinemetry-based and visual classifications, we find that the reliability of kinematic classification shows a strong trend with the interaction stage of galaxies. Mergers with two nuclei and tidal tails have the most distinct kinematic properties compared to isolated disks, whereas a significant population of the interacting disks and merger remnants are indistinguishable from isolated disks. The high fraction of late-stage mergers showing disk-like kinematics reflects the complexity of the dynamics during galaxy interactions. However, the exact fractions of misidentified disks and mergers depend on the definition of kinematic asymmetries and the classification threshold when using kinemetry-based classifications. Our results suggest that additional indicators such as morphologies traced by stars or molecular gas are required to further constrain the merger/disk classifications at high-z.Comment: 16 pages, 5 figures, ApJ accepte

Effects of neutrino oscillations and neutrino magnetic moments on elastic neutrino-electron scattering

We consider elastic antineutrino-electron scattering taking into account possible effects of neutrino masses and mixing and of neutrino magnetic moments and electric dipole moments. Having in mind antineutrinos produced in a nuclear reactor we compute, in particular, the weak-electromagnetic interference terms which are linear in the magnetic (electric dipole) moments and also in the neutrino masses. We show that these terms are, however, suppressed compared to the pure weak and electromagnetic cross section. We also comment upon the possibility of using the electromagnetic cross section to investigate neutrino oscillations.Comment: 12 pages, REVTEX file, no figures, submitted to Phys.Rev.

Two superluminous supernovae from the early universe discovered by the Supernova Legacy Survey

We present spectra and lightcurves of SNLS 06D4eu and SNLS 07D2bv, two hydrogen-free superluminous supernovae discovered by the Supernova Legacy Survey. At z = 1.588, SNLS 06D4eu is the highest redshift superluminous SN with a spectrum, at M_U = -22.7 is one of the most luminous SNe ever observed, and gives a rare glimpse into the restframe ultraviolet where these supernovae put out their peak energy. SNLS 07D2bv does not have a host galaxy redshift, but based on the supernova spectrum, we estimate it to be at z ~ 1.5. Both supernovae have similar observer-frame griz lightcurves, which map to restframe lightcurves in the U-band and UV, rising in ~ 20 restframe days or longer, and declining over a similar timescale. The lightcurves peak in the shortest wavelengths first, consistent with an expanding blackbody starting near 15,000 K and steadily declining in temperature. We compare the spectra to theoretical models, and identify lines of C II, C III, Fe III, and Mg II in the spectrum of SNLS 06D4eu and SCP 06F6, and find that they are consistent with an expanding explosion of only a few solar masses of carbon, oxygen, and other trace metals. Thus the progenitors appear to be related to those suspected for SNe Ic. A high kinetic energy, 10^52 ergs, is also favored. Normal mechanisms of powering core- collapse or thermonuclear supernovae do not seem to work for these supernovae. We consider models powered by 56Ni decay and interaction with circumstellar material, but find that the creation and spin-down of a magnetar with a period of 2ms, magnetic field of 2 x 10^14 Gauss, and a 3 solar mass progenitor provides the best fit to the data.Comment: ApJ, accepted, 43 pages, 15 figure

Deep near-IR observations of the Globular Cluster M4: Hunting for Brown Dwarfs

We present an analysis of deep HST/WFC3 near-IR (NIR) imaging data of the globular cluster M4. The best-photometry NIR colour-magnitude diagram (CMD) clearly shows the main sequence extending towards the expected end of the Hydrogen-burning limit and going beyond this point towards fainter sources. The white dwarf sequence can be identified. As such, this is the deepest NIR CMD of a globular cluster to date. Archival HST optical data were used for proper-motion cleaning of the CMD and for distinguishing the white dwarfs (WDs) from brown dwarf (BD) candidates. Detection limits in the NIR are around F110W approx 26.5 mag and F160W approx27 mag, and in the optical around F775W approx 28 mag. Comparing our observed CMDs with theoretical models, we conclude that we have reached beyond the H-burning limit in our NIR CMD and are probably just above or around this limit in our optical-NIR CMDs. Thus, any faint NIR sources that have no optical counterpart are potential BD candidates, since the optical data are not deep enough to detect them. We visually inspected the positions of NIR sources which are fainter than the H-burning limit in F110W and for which the optical photometry did not return a counterpart. We found in total five sources for which we did not get an optical measurement. For four of these five sources, a faint optical counterpart could be visually identified, and an upper optical magnitude was estimated. Based on these upper optical magnitude limits, we conclude that one source is likely a WD, one source could either be a WD or BD candidate, and the remaining two sources agree with being BD candidates. For only one source no optical counterpart could be detected, which makes this source a good BD candidate. We conclude that we found in total four good BD candidates.Comment: ApJ accepted, 28 pages including 16 figure

An Intermediate-Mass Black Hole in the Globular Cluster G1: Improved Significance from New Keck and Hubble Space Telescope Observations

We present dynamical models for the massive globular cluster G1. The goal is to measure or place a significant upper limit on the mass of any central black hole. Whether or not globular clusters contain central massive black holes has important consequences for a variety of studies. We use new kinematic data obtained with Keck and new photometry from the Hubble Space Telescope. The Keck spectra allow us to obtain kinematics out to large radii that are required to pin down the mass-to-light ratio of the dynamical model and the orbital structure. The Hubble Space Telescope observations give us a factor of two better spatial resolution for the surface brightness profile. By fitting non-parametric, spherical, isotropic models we find a best-fit black hole mass of 1.7(+-0.3)e4 Msun. Fully general axisymmetric orbit-based models give similar results, with a black hole mass of 1.8(+-0.5)e4 Msun. The no-black hole model has Delta_chi^2=5 (marginalized over mass-to-light ratio), implying less than 3% significance. We have taken into account any change in the mass-to-light ratio in the center due to stellar remnants. These results are consistent with our previous estimate in Gebhardt, Rich & Ho (2002), and inconsistent with the analysis of Baumgardt et al. (2003) who claim that G1 does not show evidence for a black hole. These new results make G1 the best example of a cluster that contains an intermediate-mass black hole.Comment: accepted for publication in the Astrophysical Journa