The evolution of plasticity of dauer larva developmental arrest in the nematode Caenorhabditis elegans.

Organisms can end up in unfavourable conditions and to survive this they have evolved various strategies. Some organisms, including nematodes, survive unfavourable conditions by undergoing developmental arrest. The model nematode Caenorhabditis elegans has a developmental choice between two larval forms, and it chooses to develop into the arrested dauer larva form in unfavourable conditions (specifically, a lack of food and high population density, indicated by the concentration of a pheromone). Wild C. elegans isolates vary extensively in their dauer larva arrest phenotypes, and this prompts the question of what selective pressures maintain such phenotypic diversity? To investigate this we grew C. elegans in four different environments, consisting of different combinations of cues that can induce dauer larva development: two combinations of food concentration (high and low) in the presence or absence of a dauer larva-inducing pheromone. Five generations of artificial selection of dauer larvae resulted in an overall increase in dauer larva formation in most selection regimes. The presence of pheromone in the environment selected for twice the number of dauer larvae, compared with environments not containing pheromone. Further, only a high food concentration environment containing pheromone increased the plasticity of dauer larva formation. These evolutionary responses also affected the timing of the worms' reproduction. Overall, these results give an insight into the environments that can select for different plasticities of C. elegans dauer larva arrest phenotypes, suggesting that different combinations of environmental cues can select for the diversity of phenotypically plastic responses seen in C. elegans.We would like to thank Henrique Teotonio for the gift of the G140.A population, Louise Hughes and Laura Weldon for technical help, two anonymous reviewers for their comments, and NERC for funding.This is the final published version of the article. It was originally published in Ecology and Evolution (Diaz SA, Viney M, Ecology and Evolution 2015, 5(6), 1343–1353, doi:10.1002/ece3.1436) http://dx.doi.org/10.1002/ece3.143

Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in Dogs with Chronic Enteropathies

Perinuclear antineutrophil cytoplasmic antibodies (pANCA) previously have been shown to be serum markers in dogs with chronic enteropathies, with dogs that have food‐responsive disease (FRD) having higher frequencies of seropositivity than dogs with steroid‐responsive disease (SRD). The indirect immunofluorescence (IIF) assay used in previous publications is time‐consuming to perform, with low interobserver agreement. Forty‐four dogs with FRD, 20 dogs with SRD, 20 control dogs, and 38 soft‐coated wheaten terrier (SCWT) or SCWT‐cross dogs

Mass and Angular Momentum Transfer in the Massive Algol Binary RY Persei

We present an investigation of H-alpha emission line variations observed in the massive Algol binary, RY Per. We give new radial velocity data for the secondary based upon our optical spectra and for the primary based upon high dispersion UV spectra. We present revised orbital elements and an estimate of the primary's projected rotational velocity (which indicates that the primary is rotating 7 times faster than synchronous). We use a Doppler tomography algorithm to reconstruct the individual primary and secondary spectra in the region of H-alpha, and we subtract the latter from each of our observations to obtain profiles of the primary and its disk alone. Our H-alpha observations of RY Per show that the mass gaining primary is surrounded by a persistent but time variable accretion disk. The profile that is observed outside-of-eclipse has weak, double-peaked emission flanking a deep central absorption, and we find that these properties can be reproduced by a disk model that includes the absorption of photospheric light by the band of the disk seen in projection against the face of the star. We developed a new method to reconstruct the disk surface density distribution from the ensemble of H-alpha profiles observed around the orbit, and this method accounts for the effects of disk occultation by the stellar components, the obscuration of the primary by the disk, and flux contributions from optically thick disk elements. The resulting surface density distribution is elongated along the axis joining the stars, in the same way as seen in hydrodynamical simulations of gas flows that strike the mass gainer near trailing edge of the star. This type of gas stream configuration is optimal for the transfer of angular momentum, and we show that rapid rotation is found in other Algols that have passed through a similar stage.Comment: 39 pages, 12 figures, ApJ in press, 2004 June 20 issu

Wind Accretion and State Transitions in Cygnus X-1

We present the results of a spectroscopic monitoring program (from 1998 to 2002) of the H-alpha emission strength in HDE 226868, the optical counterpart of the black hole binary, Cyg X-1. The H-alpha profiles consist of (1) a P Cygni component associated with the wind of the supergiant, (2) emission components that attain high velocity at the conjunctions and that probably form in enhanced outflows both towards and away from the black hole, and (3) an emission component that moves in anti-phase with the supergiant's motion. We argue that the third component forms in accreted gas near the black hole, and the radial velocity curve of the emission is consistent with a mass ratio of M_X / M_opt = 0.36 +/- 0.05. We find that there is a general anti-correlation between the H-alpha emission strength and X-ray flux in the sense that when the H-alpha emission is strong (W_\lambda < -0.5 Angstroms) the X-ray flux is weaker and the spectrum harder. On the other hand, there is no correlation between H-alpha emission strength and X-ray flux when H-alpha is weak. During the low/hard X-ray state, the strong wind is fast and the accretion rate is relatively low, while in the high/soft state the weaker, highly ionized wind attains only a moderate velocity and the accretion rate increases. We argue that the X-ray transitions from the normal low/hard to the rare high/soft state are triggered by episodes of decreased mass loss rate in the supergiant donor star.Comment: 45 pages, 16 figures, ApJ, in pres

Technical design and performance of the NEMO3 detector

The development of the NEMO3 detector, which is now running in the Frejus Underground Laboratory (L.S.M. Laboratoire Souterrain de Modane), was begun more than ten years ago. The NEMO3 detector uses a tracking-calorimeter technique in order to investigate double beta decay processes for several isotopes. The technical description of the detector is followed by the presentation of its performance.Comment: Preprint submitted to Nucl. Instrum. Methods A Corresponding author: Corinne Augier ([email protected]

KOI-3158: The oldest known system of terrestrial-size planets

The first discoveries of exoplanets around Sun-like stars have fueled efforts to find ever smaller worlds evocative of Earth and other terrestrial planets in the Solar System. While gas-giant planets appear to form preferentially around metal-rich stars, small planets (with radii less than four Earth radii) can form under a wide range of metallicities. This implies that small, including Earth-size, planets may have readily formed at earlier epochs in the Universe's history when metals were far less abundant. We report Kepler spacecraft observations of KOI-3158, a metal-poor Sun-like star from the old population of the Galactic thick disk, which hosts five planets with sizes between Mercury and Venus. We used asteroseismology to directly measure a precise age of 11.2+/-1.0 Gyr for the host star, indicating that KOI-3158 formed when the Universe was less than 20% of its current age and making it the oldest known system of terrestrial-size planets. We thus show that Earth-size planets have formed throughout most of the Universe's 13.8-billion-year history, providing scope for the existence of ancient life in the Galaxy.Comment: Submitted to EPJ Web of Conferences, to appear in the Proceedings of the 3rd CoRoT Symposium, Kepler KASC7 joint meeting; 4 pages, 1 figur

Radial Velocities of Six OB Stars

We present new results from a radial velocity study of six bright OB stars with little or no prior measurements. One of these, HD 45314, may be a long-period binary, but the velocity variations of this Be star may be related to changes in its circumstellar disk. Significant velocity variations were also found for HD 60848 (possibly related to nonradial pulsations) and HD 61827 (related to wind variations). The other three targets, HD 46150, HD 54879, and HD 206183, are constant velocity objects, but we note that HD 54879 has H$\alpha$ emission that may originate from a binary companion. We illustrate the average red spectrum of each target.Comment: Accepted for publication in PASP July 2007 issu

Cataclysmic Variables in the First Year of the Zwicky Transient Facility

Using selection criteria based on amplitude, time, and color, we have identified 329 objects as known or candidate cataclysmic variables (CVs) during the first year of testing and operation of the Zwicky Transient Facility. Of these, 90 are previously confirmed CVs, 218 are strong candidates based on the shape and color of their light curves obtained during 3–562 days of observation, and the remaining 21 are possible CVs but with too few data points to be listed as good candidates. Almost half of the strong candidates are within 10 deg of the galactic plane, in contrast to most other large surveys that have avoided crowded fields. The available Gaia parallaxes are consistent with sampling the low mass transfer CVs, as predicted by population models. Our follow-up spectra have confirmed Balmer/helium emission lines in 27 objects, with four showing high-excitation He ii emission, including candidates for an AM CVn, a polar, and an intermediate polar. Our results demonstrate that a complete survey of the Galactic plane is needed to accomplish an accurate determination of the number of CVs existing in the Milky Way

The PLATO Dome A Site-Testing Observatory : instrumentation and first results

The PLATeau Observatory (PLATO) is an automated self-powered astrophysical observatory that was deployed to Dome A, the highest point on the Antarctic plateau, in 2008 January. PLATO consists of a suite of site-testing instruments designed to quantify the benefits of the Dome A site for astronomy, and science instruments designed to take advantage of the unique observing conditions. Instruments include CSTAR, an array of optical telescopes for transient astronomy; Gattini, an instrument to measure the optical sky brightness and cloud cover statistics; DASLE, an experiment to measure the statistics of the meteorological conditions within the near-surface layer; Pre-HEAT, a submillimeter tipping radiometer measuring the atmospheric transmission and water vapor content and performing spectral line imaging of the Galactic plane; and Snodar, an acoustic radar designed to measure turbulence within the near-surface layer. PLATO has run completely unattended and collected data throughout the winter 2008 season. Here we present a detailed description of the PLATO instrument suite and preliminary results obtained from the first season of operation