Spatial Panel Data Forecasting over Different Horizons, Cross-Sectional and Temporal Dimensions

Empirical assessments of the forecasting power of spatial panel data econometric models are still scarcely available. Moreover, several methodological contributions rely on simulated data to showcase the potential of proposed methods. While simulations may be useful to evaluate the properties of a single estimator, the empirical set-ups of simulation studies are often based on strong assumptions regarding the shape and regularity of the statistical distribution of the variables involved. It is then valuable to have, next to simulation studies, empirical assessments of competing econometric models based on real data. In this paper, we evaluate competing spatial (dynamic) panel methods, selecting a number of data sets characterized by a range of different cross-sectional and temporal dimensions, as well as different levels of spatial auto-correlation. We carry out our empirical exercise on regional unemployment data for France, Spain and Switzerland. Additionally, we test different forecasting horizons, in order to investigate the speed of deterioration of forecasting quality. We compare two classes of methods: spatial vector autoregressive (SVAR) models and dynamic panel models making use of eigenvector spatial filtering (SF). We find that, as it could be expected, the unbalance between the temporal and cross-sectional dimension (T>>n) does play in favour of the SVAR model. On the other hand, the advantage of the SVAR model over the SF model appears to diminish as the forecasting horizon widens, eventually leading the SF model to being preferred for more distant forecasts

Molecular basis of contact inhibition of locomotion

Contact inhibition of locomotion (CIL) is a complex process, whereby cells undergoing a collision with another cell cease their migration towards the colliding cell. CIL has been identified in numerous cells during development including embryonic fibroblasts, neural crest cells and haemocytes and is the driving force behind a range of phenomenon including collective cell migration and dispersion. The loss of normal CIL behaviour towards healthy tissue has long been implicated in the invasion of cancer cells. CIL is a multi-step process that is driven by the tight coordination of molecular machinery. In this review, we shall breakdown CIL into distinct steps and highlight the key molecular mechanisms and components that are involved in driving each step of this process

Efecto de la defoliación post-quema sobre el crecimiento de dos gramíneas nativas en el Caldenal, Argentina

El crecimiento en los pastizales naturales semiáridos puede ser afectado por la longitud de tiempo que el pastoreo es excluido durante el período de regeneración post-fuego. El objetivo de este trabajo a campo fue estudiar el efecto de la defoliación post-fuego en diferentes estados fenológicos: vegetativo (defoliación temprana) y elongación de entrenudos (defoliación tardía) sobre el crecimiento de Piptochaetium napostaense y Poa ligularis, dos gramíneas nativas perennes muy importantes en la región semiárida templada central de Argentina (Caldenal). En general, los tratamientos de defoliación post-quema no afectaron el número de hojas verdes en las macollas de P. napostaense. La defoliación temprana después del tratamiento de fuego generalmente redujo el número de hojas verdes en las macollas de P. ligularis con respecto a las plantas control. Contrariamente, la defoliación tardía usualmente incrementó el número de hojas verdes en las macollas de P. ligularis. Sin embargo, en ambos casos, muy pocas de las diferencias detectadas fueron significativas (p0,05) a aquellas de las plantas no defoliadas. Inmediatamente después de los tratamientos de defoliación temprana y tardía, las tasas de crecimiento relativas de la altura y la longitud verde total de las macollas de las plantas de ambas especies fueron reducidas (p0.05) than values on non-defoliated plants, respectively. Immediately after early- and late-season defoliation treatments, relative growth rates for tiller height and total green length were reduced (p<0.05) on plants of both species with respect to control plants. Our results suggest that a one-year-period without severe defoliations after fire would be at least necessary for not risking the persistence of these perennial grasses in the Caldenal community.Fil: Pelaez, Daniel Valerio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Boo, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Mayor, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Elia, Omar Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Martinez, S. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin

Animal models for studying neural crest development: is the mouse different?

The neural crest is a uniquely vertebrate cell type and has been well studied in a number of model systems. Zebrafish, Xenopus and chick embryos largely show consistent requirements for specific genes in early steps of neural crest development. By contrast, knockouts of homologous genes in the mouse often do not exhibit comparable early neural crest phenotypes. In this Spotlight article, we discuss these species-specific differences, suggest possible explanations for the divergent phenotypes in mouse and urge the community to consider these issues and the need for further research in complementary systems

Characterization of Pax3 and Sox10 Transgenic Xenopus Laevis Embryos as Tools to Study Neural Crest Development

The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos

Respuesta de la vegetación a un fuego controlado en la Provincia Fitogeográfica del Monte, Argentina

El fuego es un proceso ecológico clave en la mayoría de los ecosistemas terrestres. Por lo tanto, la comprensión de la respuesta de la vegetación al fuego es esencial para su manejo. El objetivo de este trabajo fue estudiar el efecto de un fuego controlado, realizado a fines del verano, sobre la cobertura y densidad de las principales especies leñosas y herbáceas en la Provincia Fitogeográfica del Monte, Argentina. En general, después del fuego controlado el porcentaje de cobertura media de las especies leñosas fue significativamente menor (p0,05) en la densidad media de las especies leñosas a lo largo del período de estudio. La cobertura foliar media de las gramíneas perennes deseables, excepto en dos fechas de muestreo, fue significativamente mayor (p0,05) entre tratamientos en la densidad de las gramíneas perennes deseables. La densidad de las gramíneas perennes intermedias fue consistentemente menor en el tratamiento quemado que en el tratamiento control, pero sólo ocasionalmente las diferencias fueron significativas (p0.05) were detected in woody species mean density among treatments throughout the whole study. Desirable perennial grass mean percentage foliar cover, except at two sampling dates, was higher (p<0.05) in the controlled fire treatment than in the control. Contrarily, mean percentage foliar cover of intermediate perennial grasses was lower in the fire treatment than in the control, although differences were not always significant (p<0.05). No significant differences (p<0.05) in desirable perennial grass density were detected among treatments. Intermediate perennial grass density was consistently lower in the fire than in the control treatment, although differences were not always significant at p<0.05. Desirable annual grass and forb foliar covers were slightly higher in the fire than in the control treatment. Our results suggest that the reduction of woody species cover might have favored the foliar cover increase of the desirable perennial grasses. Moreover, our results suggest that a single, controlle burn would not seem to start a plant species replacement sequence, and that the plant community would persist with no variation in the density of the major woody species and the most prominent perennial grasses.Fil: Pelaez, Daniel Valerio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Giorgetti, Hugo Dosindo. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Montenegro, Oscar Alberto. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Elia, Omar Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Rodriguez, Gustavo Dionisio. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Boo, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Mayor, Mirta Doris. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentin

Sculpting with stiffness: rigidity as a regulator of morphogenesis

From a physical perspective, morphogenesis of tissues results from interplay between their material properties and the mechanical forces exerted on them. The importance of mechanical forces in influencing cell behaviour is widely recognised, whereas the importance of tissue material properties in vivo, like stiffness, has only begun to receive attention in recent years. In this mini-review, we highlight key themes and concepts that have emerged related to how tissue stiffness, a fundamental material property, guides various morphogenetic processes in living organisms

Actomyosin forces in cell migration: Moving beyond cell body retraction

In textbook illustrations of migrating cells, actomyosin contractility is typically depicted as the contraction force necessary for cell body retraction. This dogma has been transformed by the molecular clutch model, which acknowledges that actomyosin traction forces also generate and transmit biomechanical signals at the leading edge, enabling cells to sense and shape their migratory path in mechanically complex environments. To fulfill these complementary functions, the actomyosin system assembles a gradient of contractile energy along the front-rear axis of migratory cells. Here, we highlight the hierarchic assembly and self-regulatory network structure of the actomyosin system and explain how the kinetics of different nonmuscle myosin II (NM II) paralogs synergize during contractile force generation. Our aim is to emphasize how protrusion formation, cell adhesion, contraction, and retraction are spatiotemporally integrated during different modes of migration, including chemotaxis and durotaxis. Finally, we hypothesize how different NM II paralogs might tune aspects of migration in vivo, highlighting future research directions

Self-organized collective cell behaviors as design principles for synthetic developmental biology

Over the past two decades, molecular cell biology has graduated from a mostly analytic science to one with substantial synthetic capability. This success is built on a deep understanding of the structure and function of biomolecules and molecular mechanisms. For synthetic biology to achieve similar success at the scale of tissues and organs, an equally deep understanding of the principles of development is required. Here, we review some of the central concepts and recent progress in tissue patterning, morphogenesis and collective cell migration and discuss their value for synthetic developmental biology, emphasizing in particular the power of (guided) self-organization and the role of theoretical advances in making developmental insights applicable in synthesis