Results of ASTM round robin testing for mode 1 interlaminar fracture toughness of composite materials

The results are summarized of several interlaboratory 'round robin' test programs for measuring the mode 1 interlaminar fracture toughness of advanced fiber reinforced composite materials. Double Cantilever Beam (DCB) tests were conducted by participants in ASTM committee D30 on High Modulus Fibers and their Composites and by representatives of the European Group on Fracture (EGF) and the Japanese Industrial Standards Group (JIS). DCB tests were performed on three AS4 carbon fiber reinforced composite materials: AS4/3501-6 with a brittle epoxy matrix; AS4/BP907 with a tough epoxy matrix; and AS4/PEEK with a tough thermoplastic matrix. Difficulties encountered in manufacturing panels, as well as conducting the tests are discussed. Critical issues that developed during the course of the testing are highlighted. Results of the round robin testing used to determine the precision of the ASTM DCB test standard are summarized

Surface-sampled simulations of turbulent flow at high Reynolds number

A new approach to turbulence simulation, based on a combination of large-eddy simulation (LES) for the whole flow and an array of non-space-filling quasi-direct numerical simulations (QDNS), which sample the response of near-wall turbulence to large-scale forcing, is proposed and evaluated. The technique overcomes some of the cost limitations of turbulence simulation, since the main flow is treated with a coarse-grid LES, with the equivalent of wall functions supplied by the near-wall sampled QDNS. Two cases are tested, at friction Reynolds number Re$_\tau$=4200 and 20,000. The total grid node count for the first case is less than half a million and less than two million for the second case, with the calculations only requiring a desktop computer. A good agreement with published DNS is found at Re$_\tau$=4200, both in terms of the mean velocity profile and the streamwise velocity fluctuation statistics, which correctly show a substantial increase in near-wall turbulence levels due to a modulation of near-wall streaks by large-scale structures. The trend continues at Re$_\tau$=20,000, in agreement with experiment, which represents one of the major achievements of the new approach. A number of detailed aspects of the model, including numerical resolution, LES-QDNS coupling strategy and sub-grid model are explored. A low level of grid sensitivity is demonstrated for both the QDNS and LES aspects. Since the method does not assume a law of the wall, it can in principle be applied to flows that are out of equilibrium.Comment: Author accepted version. Accepted for publication in the International Journal for Numerical Methods in Fluids on 26 April 201

Quantitative Flow Field Imaging about a Hydrophobic Sphere Impacting on a Free Surface

This fluid dynamics video shows the impact of a hydrophobic sphere impacting a water surface. The sphere has a mass ratio of m* = 1.15, a wetting angle of 110 degrees, a diameter of 9.5 mm, and impacts the surface with a Froude number of Fr = 9.2. The first sequence shows an impact of a sphere on the free surface illustrating the formation of the splash crown and air cavity. The cavity grows both in the axial and radial direction until it eventually collapses at a point roughly half of the distance from the free surface to the sphere, which is known as the pinch-off point. The second set of videos shows a sphere impacting the free surface under the same conditions using Particle Image Velocimetry (PIV) to quantify the flow field. A laser sheet illuminates the mid-plane of the sphere, and the fluid is seeded with particles whose motion is captured by a high-speed video camera. Velocity fields are then calculated from the images. The video sequences from left to right depict the radial velocity, the axial velocity, and the vorticity respectively in the flow field. The color bar on the far left indicates the magnitude of the velocity and vorticity. All videos were taken at 2610 fps and the PIV data was processed using a 16 x 16 window with a 50% overlap.Comment: American Physical Society Division of Fluid Dynamics 2008 Annual Meeting Replaced previous version because abstract had LaTex markup and was too long, missing periods on middle initial of first two name

Structural plasticity and associative memory in balanced neural networks with spike-time dependent inhibitory plasticity

Peer reviewe

Stellar Substructures around the Hercules Dwarf Spheroidal Galaxy

We present deep $g,i$-band DECam stellar photometry of the Hercules Milky Way satellite galaxy, and its surrounding field, out to a radial distance of 5.4 times the tidal radius. We have identified nine extended stellar substructures associated with the dwarf; preferentially distributed along the major axis of the galaxy. Two significant over-densities lie outside the 95\% confidence band for the likely orbital path of the galaxy and appear to be free-floating tidal debris. We estimate the luminosity of the new stellar substructures, and find that approximately the same amount of stellar flux is lying in these extended structures as inside the main body of Hercules. We also analyse the distribution of candidate blue-horizontal-branch stars and find agreement with the alignment of the substructures at a confidence level greater than 98\%. Our analysis provides a quantitative demonstration that Hercules is a strongly tidally disrupted system, with noticeable stellar features at least 1.9 kpc away from the galaxy.Comment: 13 pages, 13 figures, accepted for publication in Ap

Crucial Role for Early Growth Response-1 in the Transcriptional Regulation of miR-20b in Breast Cancer

Transcriptional regulation of miRNAs that control the pathogenesis of breast cancer remains largely unknown. Here, we showed that ionizing radiation, a known breast carcinogen, triggered the differential expression of miR-20b in mammary tissues. We identified several GC-rich consensus binding motifs for the zinc finger transcription factor early growth response-1 (EGR1) in miR-20b promoter. miR-20b was upregulated by IR and its upregulation correlated with EGR1 expression in the breast cancer cell line HCC1806. Therefore, we used HCC1806 cells as a model system to explore the role of EGR1 in miR-20b transcription. siRNA knockdown of EGR1 attenuated miR-20b expression. Luciferase assays showed that whereas EGR1 stimulated luciferase activity driven by the wild-type miR-20b promoter, this induction was abolished in the mutant miR-20 promoter construct. We noted significant enrichment of EGR1 at miR-20b promoter in HCC1806 cells compared with normal human mammary epithelial cells. Suppression of miR-20b significantly inhibited HCC1806 cell proliferation and migration, and led to G 0/G 1 and S phase arrest. In vitro RNA-pull down assays indicated that miR-20b targets numerous tumor suppressors, including PTEN and BRCA1, which were downregulated in HCC1806. Conversely, suppression of miR-20b increased PTEN and BRCA1 levels. Moreover, immunohistochemical and FISH analyses showed that the miR-20b expression correlated significantly with EGR1 levels in breast cancer tissues. Our findings thus demonstrate for the first time that EGR1 is a key player in the transcriptional control of miR-20b, and miR-20b may in turn function as an oncogene by contributing to breast tumorigenesis via tumor suppressor targeting

Variation in hearing within a wild population of beluga whales (Delphinapterus leucas)

Author Posting. © The Company of Biologists, 2018. This article is posted here by permission of The Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Experimental Biology 221 (2018): jeb171959, doi:10.1242/jeb.171959.Documenting hearing abilities is vital to understanding a species’ acoustic ecology and for predicting the impacts of increasing anthropogenic noise. Cetaceans use sound for essential biological functions such as foraging, navigation and communication; hearing is considered to be their primary sensory modality. Yet, we know little regarding the hearing of most, if not all, cetacean populations, which limits our understanding of their sensory ecology, population level variability and the potential impacts of increasing anthropogenic noise. We obtained audiograms (5.6–150 kHz) of 26 wild beluga whales to measure hearing thresholds during capture–release events in Bristol Bay, AK, USA, using auditory evoked potential methods. The goal was to establish the baseline population audiogram, incidences of hearing loss and general variability in wild beluga whales. In general, belugas showed sensitive hearing with low thresholds (<80 dB) from 16 to 100 kHz, and most individuals (76%) responded to at least 120 kHz. Despite belugas often showing sensitive hearing, thresholds were usually above or approached the low ambient noise levels measured in the area, suggesting that a quiet environment may be associated with hearing sensitivity and that hearing thresholds in the most sensitive animals may have been masked. Although this is just one wild population, the success of the method suggests that it should be applied to other populations and species to better assess potential differences. Bristol Bay beluga audiograms showed substantial (30–70 dB) variation among individuals; this variation increased at higher frequencies. Differences among individual belugas reflect that testing multiple individuals of a population is necessary to best describe maximum sensitivity and population variance. The results of this study quadruple the number of individual beluga whales for which audiograms have been conducted and provide the first auditory data for a population of healthy wild odontocetes.Project funding and field support were provided by multiple institutions, including Georgia Aquarium, the Marine Mammal Laboratory of the Alaska Fisheries Science Center (MML/AFSC), and the Woods Hole Oceanographic Institution (Arctic Research Initiative, Ocean Life Institute and Marine Mammal Center). Field work was also supported by National Marine Fisheries Service Alaska Regional Office (NMFS AKR), U.S. Fish and Wildlife Service, Bristol Bay Native Association and Bristol Bay Marine Mammal Council, Alaska SeaLife Center, Shedd Aquarium and Mystic Aquarium. Audiogram analyses were initially funded by the Office of Naval Research award number N000141210203.2019-05-0