Aptitud anaerobia en deportistas de combate del sexo femenino

Se estudia en nivel de preparación anaerobia en tres selecciones femeninas de deportes de combate de alta calificación: 15 judocas, 7 esgrimistas de la modalidad sable y 16 taekwondoistas. Fueron medidos indicadores neuromusculares, al final de la preparación especial, utilizando los tests de ergosalto y de saltabilidad de Bosco, Se determinan las estadísticas descriptivas, significación de las diferencias entre deportes y la correlación entre indicadores. Se obtienen los resultados: Pala (15,7 ± 2,1-19,2 ± 1,5 W/Kg), Pana (14,5 ± 1,9-17,7 ± 1,2 W/Kg), Los SCAB, SSAB, SCPD, SCPCD y SDPC mas altos fueron los de las esgrimistas y la mejor coordinación se encontró en las competidoras de esgrima, mientras que los mayores valores de CELAS fueron los de las judocas. Los resultados obtenidos se corresponden con las características del gesto deportivo que prevalece en cada uno de los deportes estudiados

Environmental Factors and Breast Cancer Mortality

Breast cancer is a public health problem in the United States that impacts diverse ethnic female groups in different ways regarding incidence and mortality. Much is known about the factors increasing the risk of developing breast cancer, but little is known about those that increase the chances of dying from it, other than access to health care, screening, and appropriate treatment. This study aimed to understand the association between exposure to Environmental Protection Agency-regulated air pollutants and breast cancer mortality, including how the interaction between these air contaminants impacts the outcome of interest, considering median income, education level, and percentage of White women. The ecosocial theory and probabilistic epigenesis guided the study. These theories explain the disease development concerning the historical exposure of individuals to their environment. An ecological study design was conducted, using secondary data from the Outdoor Air Quality Data, the U.S. Cancer Statistics Data Visualizations Tool, and the U.S. Census Bureau. The results of the regression analyses indicated that particulate matter2.5, a type of inhalable particulate of less than 2.5µm; percent of adults with less than a high school diploma; and percentage of White women were statistically associated with age-adjusted breast cancer mortality. Implications for positive social change including a better understanding of the predictors of breast cancer will serve public health policymakers and government officials to make evidence-based decisions and enact rules and laws aimed at controlling air pollution

Environmental Factors and Breast Cancer Mortality

Breast cancer is a public health problem in the United States that impacts diverse ethnic female groups in different ways regarding incidence and mortality. Much is known about the factors increasing the risk of developing breast cancer, but little is known about those that increase the chances of dying from it, other than access to health care, screening, and appropriate treatment. This study aimed to understand the association between exposure to Environmental Protection Agency-regulated air pollutants and breast cancer mortality, including how the interaction between these air contaminants impacts the outcome of interest, considering median income, education level, and percentage of White women. The ecosocial theory and probabilistic epigenesis guided the study. These theories explain the disease development concerning the historical exposure of individuals to their environment. An ecological study design was conducted, using secondary data from the Outdoor Air Quality Data, the U.S. Cancer Statistics Data Visualizations Tool, and the U.S. Census Bureau. The results of the regression analyses indicated that particulate matter2.5, a type of inhalable particulate of less than 2.5µm; percent of adults with less than a high school diploma; and percentage of White women were statistically associated with age-adjusted breast cancer mortality. Implications for positive social change including a better understanding of the predictors of breast cancer will serve public health policymakers and government officials to make evidence-based decisions and enact rules and laws aimed at controlling air pollution

Microsurgical Limb Reconstruction Utilizing Truelok TL External Fixator: A Case Report

Coverage of lower extremity wounds, especially those in the distal leg, present challenges to the reconstructive surgeon. The present case illustrates a surgical technique utilizing a distally based reverse soleus muscle flap for coverage of an anterior leg wound deficit with exposed bone. The wound failed conservative wound care and was at risk of a below the knee amputation. The wound was first debrided to healthy bleeding tissue. The Truelok TL External Fixator was then applied for stabilization of the muscle flap. The medial portion of the soleus muscle was dissected with care to preserve its vascular supply and transposed to cover the wound defect. This was followed by utilization of the Integra Bi-Layer Matrix to control the vapor loss of the wound, act as a bacterial barrier, and provide a scaffold for cellular invasion and capillary growth. A wound VAC was applied to promote granular tissue formation. Following post-operative wound care, a split-thickness skin graft was later applied. The limb was salvaged and wound closure was achieved within three months. The patient began ambulating in a patella tendon bearing orthosis within four months. The reverse soleus muscle flap provides a viable option for ankle wound and anterior leg coverage, especially in medically frail patients. Due to a high degree of versatility, reliability, minimal donor site morbidity, less operating time, low cost and good functional gain; this procedure is highly suitable for the treatment of complex middle and lower leg defects. It should be considered in the reconstruction of soft tissue defects about the ankle, especially when the surgeon has exhausted all other conservative and surgical options

Violencia de género: Un acercamiento a su expresión simbólica en mujeres afromexicanas

"El presente artículo tiene como objetivo realizar un acercamiento a la violencia de género, con especial atención en su manifestación simbólica en las mujeres afromexicanas. Nos apoyamos en la metodología cualitativa y el método hermenéutico a fin de comprender e intentar esbozar algunas interpretaciones de este fenómeno. Se revisaron y analizaron los marcos conceptuales y teóricos de intelectuales que en sus estudios han reflexionado sobre la categoría de género, la vinculación de este con la reproducción de la violencia, la problematización acerca de la “raza” y la importancia del enfoque interseccional en el develamiento de las matrices de dominación. El análisis expone que la violencia simbólica hacia las afromexicanas se entrelaza con aspectos como la desigualdad de género, el no reconocimiento, su invisibilización histórica, la discriminación racista, el racismo estructural e institucional. Se pretende lograr una mayor concientización y reconocimiento de las maneras en que se configuran las dinámicas en torno a las violencias y afrodescendencias mexicanas y brindar así elementos para su visibilización y prevención.

HDAC7 Is a Repressor of Myeloid Genes Whose Downregulation Is Required for Transdifferentiation of Pre-B Cells into Macrophages

B lymphopoiesis is the result of several cell-commitment, lineage-choice, and differentiation processes. Every differentiation step is characterized by the activation of a new, lineage-specific, genetic program and the extinction of the previous one. To date, the central role of specific transcription factors in positively regulating these distinct differentiation processes to acquire a B cell-specific genetic program is well established. However, the existence of specific transcriptional repressors responsible for the silencing of lineage inappropriate genes remains elusive. Here we addressed the molecular mechanism behind repression of non-lymphoid genes in B cells. We report that the histone deacetylase HDAC7 was highly expressed in pre-B cells but dramatically down-regulated during cellular lineage conversion to macrophages. Microarray analysis demonstrated that HDAC7 re-expression interfered with the acquisition of the gene transcriptional program characteristic of macrophages during cell transdifferentiation; the presence of HDAC7 blocked the induction of key genes for macrophage function, such as immune, inflammatory, and defense response, cellular response to infections, positive regulation of cytokines production, and phagocytosis. Moreover, re-introduction of HDAC7 suppressed crucial functions of macrophages, such as the ability to phagocytose bacteria and to respond to endotoxin by expressing major pro-inflammatory cytokines. To gain insight into the molecular mechanisms mediating HDAC7 repression in pre-B cells, we undertook co-immunoprecipitation and chromatin immunoprecipitation experimental approaches. We found that HDAC7 specifically interacted with the transcription factor MEF2C in pre-B cells and was recruited to MEF2 binding sites located at the promoters of genes critical for macrophage function. Thus, in B cells HDAC7 is a transcriptional repressor of undesirable genes. Our findings uncover a novel role for HDAC7 in maintaining the identity of a particular cell type by silencing lineage-inappropriate genes

Workflow for high definition documentation of the roman archaeological site of Herrera (Seville)

Structure from Motion (SfM) photogrammetry has become in the last years one of the most used methodologies for three-dimensional recording in many scientific fields thanks to its great precision, low cost and speed. The aim of this paper is to present a workflow methodology to obtain precise results for the graphical documentation of an archaeological site with complex structures (Rouco &amp; Benavides, 2023)The improvement of photogrammetric hardware and software has provided us with a tool of great value for archaeological research and survey. SfM photogrammetry has been widely applied to cultural heritage studies in a variety of ways, such as digitizing historical buildings, archaeological sites and artefacts. Improvement in UAV-mounted camera technology and calibration methods of non-metric cameras have expanded the use of photogrammetric modelling to non-professional and semi-professional users (Westoby et al. 2012). Although the potential of SfM photogrammetry allows obtaining 3D models with a very good graphic appearance using almost any type of camera, these models need certain calibration and adjustment processes in order to achieve enough precision for professional use.This paper focuses on discussing some important data capture workflow recommendations, as well as the new processing workflows implemented in Agisoft Metashape version 2.0, which improve 3D digital reconstruction and graphical documentation to make it complete and precise.The Herrera Roman Baths site offers an excellent opportunity to explore the complex casuistry in the record of cultural heritage due to the complexity in the registration of the structures and the high degree of precision and graphic resolution that the project required for the tasks of restoration of decorative elements. The existence of a roof structure that protects them has forced us to carry out a manual flight with a UAV at a very low altitude (4m), and to develop different modeling strategies to guarantee metric precision and a high level of detail.DOI: https://doi.org/10.20365/disegnarecon.30.2023.2</p

Resultados en el tratamiento quirúrgico de la úlcera gastroduodenal perforada

Fundamento: En la actualidad el tratamiento de la úlcera gastroduodenal perforada ha experimentado importantes cambios; pues no sería indicado la cirugía antisecretora ni resectiva, ya que se puede conseguir el mismo efecto con los inhibidores de la bomba de protones y con la erradicación del Helicobacter pylori. La recidiva es infrecuente, aunque en ocasiones con la cirugía no definitiva los síntomas reaparecen y hasta surgen nuevas perforaciones. Objetivo: Describir los resultados obtenidos en el tratamiento quirúrgico de la úlcera gastroduodenal perforada. Métodos: La investigación fue desarrollada en el Servicio de Cirugía General del Hospital Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, entre enero del año 2008 y mayo del año 2009, realizándose un estudio descriptivo sobre una serie de 55 casos con diagnóstico de úlcera gastroduodenal perforada, que fueron intervenidos quirúrgicamente. Resultados: El 31,2 % de los pacientes que presentaron úlcera gastroduodenal, había recibido previamente terapia anti Helicobacter pylori, siendo la perforación el debut de la enfermedad en el 45,5 % de los casos. A pesar de presentar como antecedente alguna enfermedad digestiva, el 41,7 % de estos pacientes consumía algún tipo de medicamento ulcerogénico. Las técnicas quirúrgicas más utilizadas fueron las no definitivas, predominando la sutura y epiploplastia no pediculada (78,2 %). Se encontró persistencia de la úlcera en el 20,0 % de los pacientes a los que se pudo dar seguimiento. Conclusión: Después de tres meses de evolución, algunos de los pacientes operados con una técnica quirúrgica no definitiva y terapia anti-Helicobacter pylori no resolvieron su problema de salud

Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications

Post-translational modifications (PTMs) of histones play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these processes. Linking specific histone PTMs to gene expression is an arduous task requiring large amounts of highly purified and natively modified histones to be analyzed by various techniques. We have developed robust and complementary procedures, which use strong protein denaturing conditions and yield highly purified core and linker histones from unsynchronized proliferating, M-phase arrested and butyrate-treated cells, fully preserving their native PTMs without using enzyme inhibitors. Cell hypotonic swelling and lysis, nuclei isolation/washing and chromatin solubilization under mild conditions are bypassed to avoid compromising the integrity of histone native PTMs. As controls for our procedures, we tested the most widely used conventional methodologies and demonstrated that they indeed lead to drastic histone dephosphorylation. Additionally, we have developed methods for preserving acid-labile histone modifications by performing non-acid extractions to obtain highly purified H3 and H4. Importantly, isolation of histones H3, H4 and H2A/H2B is achieved without the use of HPLC. Functional supercoiling assays reveal that both hyper- and hypo-phosphorylated histones can be efficiently assembled into polynucleosomes. Notably, the preservation of fully phosphorylated mitotic histones and their assembly into polynucleosomes should open new avenues to investigate an important but overlooked question: the impact of mitotic phosphorylation in chromatin structure and function

Mortalidad por cáncer bucal en La Habana, 2001-2010

Introduction: Oral cancer is associated with a significant morbidity and mortality. Objective: Determine the levels of mortality in Havana province from 2001 to 2010. Method: A descriptive study on oral cancer mortality was carried out in the population of Havana from 2001 to 2010. Deaths caused by cancer were included as the basic cause, according to the Center for Population and Development Studies of the National Bureau of Statistics of the Republic of Cuba and the health directing office in Havana. Variables: years, age, sex, localization and municipalities. Indicators: crude rate and adjusted rate; specific rate per sex; per groups of ages, variation of mortality percentage, crude rate of mortality and standard reason of mortality. Results: The oral cancer mortality percentage increased, the female rate was higher in 2001 compared to 2010. The most common site was the base of the tongue. The largest rate decrease was in Habana Vieja municipality (-3.73%). Conclusions: In general increased crude mortality rates per oral cancer and sex may be associated with changes in the structure of the province population; the location does not differ from that reported in the country and in the reviewed literature; the change in population structure, population size and its changes affect the behavior of mortality rates in municipalities.Introducción: el cáncer bucal está asociado con una morbilidad y mortalidad significativas. Objetivo: determinar los niveles de mortalidad en la provincia La Habana en el periodo 2001-2010. Método: se realizó un estudio descriptivo sobre la mortalidad por cáncer bucal en la población de La Habana desde 2001 hasta 2010. Se incluyeron las defunciones por cáncer como causa básica de muerte, según el Centro de Estudios de Población y Desarrollo de la Oficina Nacional de Estadísticas de la República de Cuba y la dirección sectorial de salud de la provincia La Habana. Variables: años, edad, sexo, localización y municipios. Indicadores: Tasa cruda y ajustada; Tasa específica por sexo; Tasa específica por grupos de edades, Variación porcentual de la mortalidad, Tasa bruta de mortalidad y Razón estandarizada de mortalidad. Resultados: el porcentaje de mortalidad por cáncer bucal aumentó, la tasa en el sexo femenino fue mayor en 2001 respecto al 2010. La localización más frecuente fue la base de lengua. El mayor decrecimiento en la tasa lo tuvo el municipio Habana Vieja con -3,73 %. Conclusiones: el aumento de las tasas brutas de mortalidad por cáncer bucal en general y por sexo puede estar asociado a los cambios ocurridos en la estructura de la población de la provincia; la localización no difiere de lo reportado en el país y en la literatura consultada; la variación de la estructura poblacional, la cantidad de población y sus modificaciones influyen en el comportamiento de las tasas de mortalidad de los municipios