IMECE2008-67934 TEMPERATURE-DEPENDENT PERMEABILITY OF MICROPOROUS MEMBRANES FOR VAPOR VENTING HEAT EXCHANGERS

ABSTRACT Improved flow regime stability and lower pressure drop may be possible in two-phase microfluidic heat exchangers through the use of a hydrophobic membrane for phase separation. Past research on vapor-venting heat exchangers showed that membrane mechanical and hydrodynamic properties are crucial for heat exchanger design. However, previous characterizations of hydrophobic membranes were primarily carried out at room temperatures with air or nitrogen, as opposed to liquid water and steam at the elevated operating temperature of the heat exchangers. This work investigates laminated PTFE, unlaminated PTFE, and nylon membranes and quantifies the permeability of the membranes to air and steam. The pressure drop across the membrane as a function of fluid flow rate and temperature characterizes the membrane permeability. This work will facilitate more focused experimental work and predictive modeling on optimizing membrane properties and will help with the development of more effective vapor venting heat exchangers

Increasing Hybridization Rate and Sensitivity of Bead-Based Assays Using Isotachophoresis

マイクロ流体技術を用いた網羅的・高速DNA分析技術の開発に成功 -網羅的・短時間・高感度のバイオマーカ検出技術の確立に期待-. 京都大学プレスリリース. 2014-10-15.We present an electrokinetic technique to increase the reaction rate and sensitivity of bead-based assays. We use isotachophoresis (ITP) to preconcentrate and co-focus target molecules and beads into a single ITP zone. The process achieves rapid mixing, stirring, and strongly increases the binding reaction rate. We demonstrate our assay with quantitative detection of 24 nt single-stranded DNA over a dynamic range of three orders of magnitude and multiplexed detection of ten target species per sample. We show that ITP can achieve approximately the same sensitivity as a well-stirred standard reaction in 60-fold reduced reaction time (20 min versus 20 h). Alternately, compared to standard reaction times of 30 min, we show that 20 min ITP hybridization can achieve 5.3-fold higher sensitivity