Standing on Academic Shoulders: Measuring Scientific Influence in Universities

This article measures scientific influence by means of citations to academic papers. The data source is the Institute for Scientific Information (ISI); the scientific institutions included are the top 110 U.S. research universities; the 12 main fields that classify the data cover nearly all of science; and the time period is 1981-1999. Altogether the database includes 2.4 million papers and 18.8 million citations. Thus the evidence underlying our findings accounts for much of the basic research conducted in the United States during the last quarter of the 20th century. This research in turn contributes a significant part of knowledge production in the U.S. during the same period. The citation measure used is the citation probability, which equals actual citations divided by potential citations, and captures average utilization of cited literature by individual citing articles. The mean citation probability within fields is on the order of 10-5. Cross-field citation probabilities are one-tenth to one-hundredth as large, or 10-6 to 10-7. Citations between pairs of citing and cited fields are significant in less than one-fourth of the possible cases. It follows that citations are largely bounded by field, with corresponding implications for the limits of scientific influence. Cross-field citation probabilities appear to be symmetric for mutually citing fields. Scientific influence is asymmetric within fields, and occurs primarily from top institutions to those less highly ranked. Still, there is significant reverse influence on higher-ranked schools. We also find that top institutions are more often cited by peer institutions than lower-ranked institutions are cited by their peers. Overall the results suggest that knowledge spillovers in basic science research are important, but are circumscribed by field and by intrinsic relevance. Perhaps the most important implication of the results are the limits that they seem to impose on the returns to scale in the knowledge production function for basic research, namely the proportion of available knowledge that spills over from one scientist to another.

Evaluation of housekeeping genes in Listeria monocytogenes as potential internal control references for normalizing mRNA expression levels in stress adaptation models using real-time PCR

Listeria monocytogenes is an important food-borne pathogen that can tolerate a wide range of stress conditions. However, its stress adaptation processes are still poorly understood. Real-time-based quantitative RT-PCR (qRT-PCR) provides a tool to probe gene expression changes underlying stress adaptation. But, a limitation to study mRNA levels by real-time qRT-PCR is that validated reference genes are required for normalization. Such genes are currently lacking for experimental models that may be applied to evaluate stress-related gene expression changes in L. monocytogenes. Therefore, five housekeeping genes (HKG) were studied as potential reference genes. Their expression stability was evaluated across 16 L. monocytogenes strains. Three experimental models designed to assess gene expression changes induced by cold, acid and high NaCl concentration stress adaptation were applied. The 16S rRNA gene was consistently the most stably expressed HKG across the different L. monocytogenes strains under all the experimental conditions. While the expressions of β-glucosidase (bglA), Glyceraldehyde-3P-dehydrogenase (gap), RNA polymerase beta subunit (rpoB) and Ribosomal protein L4 (rplD) was stable amongst the different L. monocytogenes strains, they were prone to significant variations under the different stress adaptation model

Complete nucleotide sequence of pVQS1 containing a quinolone resistance determinant from Salmonella enterica serovar Virchow associated with foreign travel

Objectives Nalidixic acid-resistant Salmonella enterica serovars Kentucky (n = 5) and Virchow (n = 6) cultured from individuals were investigated for the presence of plasmid-mediated quinolone resistance (PMQR) determinants. Methods PMQR markers and mutations within the quinolone resistance-determining regions of the target genes were investigated by PCR followed by DNA sequencing. Conjugation, plasmid profiling and targeted PCR were performed to demonstrate the transferability of the qnrS1 gene. Subsequently, a plasmid was identified that carried a quinolone resistance marker and this was completely sequenced. Results A Salmonella Virchow isolate carried a qnrS1 gene associated with an IncN incompatibility group conjugative plasmid of 40 995 bp, which was designated pVQS1. The latter conferred resistance to ampicillin and nalidixic acid and showed sequence similarity in its core region to plasmid R46, whilst the resistance-encoding region was similar to pAH0376 from Shigella flexneri and pINF5 from Salmonella Infantis and contained an IS26 remnant, a complete Tn3 structure, a truncated IS2 element and a qnrS1 marker, followed by IS26. In contrast to pINF5, IS26 was identified immediately downstream of the qnrS1 gene. Conclusions This is the first known report of a qnrS1 gene in Salmonella spp. in Switzerland. Analysis of the complete nucleotide sequence of the qnrS1-containing plasmid showed a novel arrangement of this antibiotic resistance-encoding regio

Resistance profiles and genetic diversity of Escherichia coli strains isolated from acute bovine mastitis

Between March 2011 and February 2012 83 E. coli strains were isolated from mastitis milk samples from 83 different animals (67 farms) and tested for their sensitivity to various antibiotics by means of disk diffusion method and genotyped by determination of the phylogenetic groups as well as by pulsed field gel electrophoresis (PFGE). The antibiotics were chosen on the basis of their licenses for intramammary application in Switzerland. As many as 16.9 % of the isolates were resistant to one or more antimicrobial agents. Amoxicillin-clavulanic acid, gentamicin and third generation cephalosporins proved effective against the majority of these strains. Nevertheless, one blaCTX-M-14 harbouring extended-spectrum-beta-lactamase producing strain was found. Genetic analysis grouped most of the strains (87 %) into phylogenetic groups A and B1. PFGE genotyping demonstrated that E. coli from cows with mastitis even from the same farm were genotypically very diverse. = Entre mars 2011 et février 2012, 83 souches d'E. coli issues de 83 vaches différentes provenant de 67 exploitations ont été collectées et testées quant à leur sensibilité vis-à-vis de divers antibiotiques. Ces antibiotiques ont été choisis sur la base de leurs autorisations pour l'application intra mammaire en Suisse et le test a été effectué par diffusion sur gel d'agar. En outre toutes les souches ont été typisées quant à leur appartenance aux groupes phylogénétiques. 16.9 % des souches présentaient une résistance à un ou plusieurs antibiotiques. L'amoxicilline-acide clavulanique, la gentamicine et les céphalosporines de troisièmes générations se montraient efficaces contre la majorité des souches d'E. coli. On a toutefois trouvé une souche fabriquant un extended-spectrum-beta-lactamase qui portait le gène blaCTX-M-14. L'analyse génétique groupait la majorité des souches (87 %) dans les groupes phylogénétiques A et B1. La génotypisation par PFGE montrait une grande diversité entre les souches, même si elles provenaient de la même exploitation. = Zwischen März 2011 und Februar 2012 wurden 83 E. coli Stämme von 83 verschiedenen Kühen aus 67 Betrieben gesammelt und auf ihre Empfindlichkeit gegenüber verschiedenen Antibiotika getestet. Die Antibiotika wurden aufgrund der Zulassung für eine intramammäre Applikation in der Schweiz ausgesucht und die Empfindlichkeitstestung mittels Agardiffusions-Methode durchgeführt. Zudem wurden alle Stämme hinsichtlich ihrer Zugehörigkeit zu den phylogenetischen Gruppen wie auch mittels Pulsfeldgelelektrophorese (PFGE) genotypisiert. 16.9 % aller Stämme zeigten Resistenzen gegenüber einem oder mehreren Antibiotika. Amoxicillin-Clavulansäure, Gentamicin und Cephalosporine der dritten Generation erwiesen sind als wirksam gegen die Mehrheit der E. coli Stämme. Jedoch wurde ein extended-Spectrum-beta-Lactamase Bildner, welcher das blaCTX-M-14-Gen trägt, gefunden. Die genetische Analyse gruppierte das Gros der Stämme (87 %) in die phylogentischen Guppen A und B1. Die weitere Genotypisierung mittels PFGE zeigte eine grosse Diversität unter den E. coli Stämmen, auch wenn diese vom selben Betrieb stammten

Draft genome sequence of Staphylococcus aureus 1608, a strain that caused toxic mastitis in twin cows

Staphylococcus aureus 1608 is a strain that caused a lethal mastitis in cows. Here, the draft genome sequence of the strain is presented

New Procedures for Uranium Isotope Ratio Measurements using the Triton Thermal Ionization Mass Spectrometer

In February 2004 the new Triton Thermal Ionization Mass Spectrometer from Thermo Electron has been installed at IRMM. For uranium isotope ratio measurements on the Triton at IRMM two basic techniques have been introduced and validated, namely the MTE- (¿Modified Total Evaporation¿) and the HI (¿High Intensity¿) techniques. The performance of these techniques is considered excellent for uranium isotope ration measurements to be performed at IRMM. Data obtained for the IRMM-183-187 series will be used to recertify the minor uranium ratios for these well know reference materialsJRC.D.4-Isotope measurement

Neutrino-driven winds from neutron star merger remnants

We present a detailed, 3D hydrodynamics study of the neutrino-driven winds that emerge from the remnant of a NS merger. Our simulations are performed with the Newtonian, Eulerian code FISH, augmented by a detailed, spectral neutrino leakage scheme that accounts for heating due to neutrino absorption in optically thin conditions. Consistent with the 2D study of Dessart et al. (2009), we find that a strong baryonic wind is blown out along the original binary rotation axis within $100$ ms after the merger. We compute a lower limit on the expelled mass of $3.5 \times 10^{-3} M_{\odot}$, large enough to be relevant for heavy element nucleosynthesis. The physical properties vary significantly between different wind regions. For example, due to stronger neutrino irradiation, the polar regions show substantially larger $Y_e$ than those at lower latitudes. This has its bearings on the nucleosynthesis: the polar ejecta produce interesting r-process contributions from $A\sim 80$ to about 130, while the more neutron-rich, lower-latitude parts produce also elements up to the third r-process peak near $A\sim 195$. We also calculate the properties of electromagnetic transients that are powered by the radioactivity in the wind, in addition to the macronova transient that stems from the dynamic ejecta. The high-latitude (polar) regions produce UV/optical transients reaching luminosities up to $10^{41} {\rm erg \, s^{-1}}$, which peak around 1 day in optical and 0.3 days in bolometric luminosity. The lower-latitude regions, due to their contamination with high-opacity heavy elements, produce dimmer and more red signals, peaking after $\sim 2$ days in optical and infrared. Our numerical experiments indicate that it will be difficult to infer the collapse time-scale of the HMNS to a BH based on the wind electromagnetic transient, at least for collapse time-scales larger than the wind production time-scale.Comment: 25 pages, 4 tables, 22 figures. Submitted to MNRA