Circadian rhythms and post-transcriptional regulation in higher plants

The circadian clock of plants allows them to cope with daily changes in their environment. This is accomplished by the rhythmic regulation of gene expression, in a process that involves many regulatory steps. One of the key steps involved at the RNA level is post-transcriptional regulation, which ensures a correct control on the different amounts and types of mRNA that will ultimately define the current physiological state of the plant cell. Recent advances in the study of the processes of regulation of pre-mRNA processing, RNA turn-over and surveillance, regulation of translation, function of lncRNAs, biogenesis and function of small RNAs, and the development of bioinformatics tools have helped to vastly expand our understanding of how this regulatory step performs its role. In this work we review the current progress in circadian regulation at the post-transcriptional level research in plants. It is the continuous interaction of all the information flow control post-transcriptional processes that allow a plant to precisely time and predict daily environmental changes.Fil: Romanowski, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin

A unique high intensity solar simulation system

This simulator is unique in that it is capable of producing intensities up to 16.0 solar constants (25 solar constants without spectral filters) and closely simulates the solar spectrum over a 12 inch diameter area. The simulator is described and the capabilities, calibration, operational experiences, data collection, and safety considerations associated with this simulator are discussed

Development of an apparatus to measure thermophysical properties of wind tunnel heat transfer models

The apparatus and technique for measuring the thermophysical properties of models used with the phase-change paint method for obtaining wind tunnel heat transfer data are described. The method allows rapid measurement of the combined properties in a transient manner similar to an actual wind tunnel test. An effective value of the thermophysical properties can be determined which accounts for changes in thermal properties with temperature or with depth into the model surface. The apparatus was successfully tested at various heating rates between 19,000 and 124,000 watts per square meter

Investigating the 'latent' deficit hypothesis : age at time of head injury, executive and implicit functions and behavioral insight

This study investigated the 'latent deficit' hypothesis in two groups of frontotemporal headinjured patients, those injured prior to steep morphological and corresponding functional maturational periods for frontotemporal networks (≤ age 25), and those injured >28 years. The latent deficit hypothesis proposes that early injuries produce enduring cognitive deficits manifest later in the lifespan with graver consequences for behavior than adult injuries, particularly after frontal pathology (Eslinger, Grattan, Damasio & Damasio, 1992). Implicit and executive deficits both contribute to behavioral insight after frontotemporal head injury (Barker, Andrade, Romanowski, Morton & Wasti, 2006). On the basis of morphological and behavioral data, we hypothesised that early injury would confer greater vulnerability to impairment on tasks associated with frontotemporal regions than later injury. Patients completed experimental tasks of implicit cognition, executive function measures and the DEX measure of behavioural insight (Behavioral Assessment of the Dysexecutive Syndrome: Wilson, Alderman, Burgess, Emslie, & Evans, 1996). The Early Injury group were more impaired on implicit cognition tasks compared to controls that Late Injury patients. There were no marked group differences on most executive function measures. Executive ability only contributed to behavioral awareness in the Early Injury Group. Findings showed that age at injury moderates the relationship between executive and implicit cognition and behavioral insight and that early injuries result in longstanding deficits to functions associated with frontotemporal regions partially supporting the latent deficit hypothesis

Implicit cognition is impaired and dissociable in a head-injured group with executive deficits

Implicit or non-conscious cognition is traditionally assumed to be robust to pathology but Gomez-Beldarrain et al (1999, 2002) recently showed deficits on a single implicit task after head injury. Laboratory research suggests that implicit processes dissociate. This study therefore examined implicit cognition in 20 head-injured patients and age- and I.Q.-matched controls using a battery of four implicit cognition tasks: a Serial Reaction Time task (SRT), mere exposure effect task, automatic stereotype activation and hidden co-variation detection. Patients were assessed on an extensive neuropsychological battery, and MRI scanned. Inclusion criteria included impairment on at least one measure of executive function. The patient group was impaired relative to the control group on all the implicit cognition tasks except automatic stereotype activation. Effect size analyses using the control mean and standard deviation for reference showed further dissociations across patients and across implicit tasks. Patients impaired on implicit tasks had more cognitive deficits overall than those unimpaired, and a larger Dysexecutive Self/Other discrepancy (DEX) score suggesting greater behavioural problems. Performance on the SRT task correlated with a composite measure of executive function. Head-injury thus produced heterogeneous impairments in the implicit acquisition of new information. Implicit activation of existing knowledge structures appeared intact. Impairments in implicit cognition and executive function may interact to produce dysfunctional behaviour after head-injury. Future comparisons of implicit and explicit cognition should use several measures of each function, to ensure that they measure the latent variable of interest

Identificación de un aislamiento argentino de Spodoptera frugiperda granulovirus

La oruga militar tardía, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), es una plaga importante del maíz. Debido al impacto ambiental y a la aparición de resistencia causados por los pesticidas químicos y los eventos transgénicos, el uso de baculovirus resulta una alternativa útil y saludable para su control en estrategias de manejo integrado de plagas. En este trabajo reportamos la identificación de un nuevo aislamiento del granulovirus de la S. frugiperda nativo de la región central de Argentina, SfGV ARG. Se observó que larvas infectadas con SfGV ARG mostraron coloración amarillenta, hinchazón y, en algunos casos, lesiones graves en los últimos segmentos abdominales. Se confirmó la identidad del aislamiento por secuenciación de fragmentos de los genes lef-8, lef-9 y granulina, y por cálculo de distancias evolutivas usando el parámetro de Kimura-2. El patrón de restricción generado con el ADN genómico de SfGV ARG permitió estimar un tamaño de genoma de al menos 135 kb.The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important maize pest. Due to the environmental impact and emergence of resistance caused by chemical pesticides and transgenic events, the use of baculoviruses becomes a safe and useful alternative for its control in integrated pest management strategies. Here we report the identification of a novel isolate of a granulovirus of S. frugiperda native to the central region of Argentina, named SfGV ARG. We observed that larvae infected with SfGV ARG showed a yellowish coloration, swollen body and, in some cases, severe lesions in the last abdominal segments. We confirmed the identity of the isolate by sequencing fragments of the lef-8, lef-9 and granulin genes and by calculating evolutionary distances using the Kimura-2-Parameter model. SfGV ARG DNA restriction pattern allowed to estimate a genome of at least 135 kb.Fil: Pidre, Matias Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Sabalette, Karina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ferrelli, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

Telemedizin und AAL in Deutschland: Geschichte, Stand und Perspektiven

In der Theorie sind sich viele Gesundheitsexperten einig, dass ein größerer Einsatz der Telemedizin enorme gesellschaftliche und wirtschaftliche Vorteile mit sich bringen könnte. Trotzdem sind viele telemedizinische Dienste und Projekte immer noch kein Standard im medizinischen Alltag, sondern meist Pilotprojekte, die nach Ende der Finanzierungszeit nicht fortgeführt werden. Im Bereich der Telemedizin existieren viele einzelne Lösungen mit bottom-up Charakter, wodurch die nötige systematische Übersicht des sich schnell verändernden Marktes erschwert wird. Es gibt derzeit keine kostenlose, bundesweite Datenbank mit systematischer Erfassung geförderter Gesundheits-Telematikprojekte nach einem festen Kriterienkatalog

Próba interpretacji koncepcji prawa globalnego

The authors reconstruct the key elements of the conception of global law proposed by Rafael Domingo, a world-recognised Spanish Roman law specialist, offer its interpretation, as well as recommend application of that law in the creation of a conception of material sources of law. The starting point for the discussion on the above is a critical assessment of subject-reductionism in public international law, and the principles of territoriality and sovereignty in particular. The authors argue that overcoming the current regulatory and academic crisis in international law will not be possible unless its existing paradigm is changed. Anthroparchy – a term coined by the authors – should become a constructive element of the new paradigm, meaning that a person with its intrinsic dignity and equality should be put in place of the state’s sovereignty. The characteristics of such person, relevant for the axiological conditioning of the legal system, is then proposed, introducing to the legal deliberations an interdisciplinary approach to philosophical anthropology. The presented conception belongs to non-positivism in law, and its explanatory force concerns questions of law’s teleology and autonomy with regard to state.Autorzy rekonstruują podstawowe elementy koncepcji prawa globalnego zaproponowanej przez Rafaela Dominga, światowej sławy hiszpańskiego romanistę, oraz proponują jej interpretację i wykorzystanie do stworzenia koncepcji materialnych źródeł prawa. Punktem wyjścia tej koncepcji jest krytyka faktu, że w dominującym nurcie nauki prawa międzynarodowego publicznego zagadnienia dotyczące podmiotowości zredukowane są niemal wyłącznie do państw. Ten redukcjonizm wspiera się w szczególności na zasadach terytorialności i suwerenności. Autorzy twierdzą, że przezwyciężenie aktualnego kryzysu regulacyjnego, związanego z procesami globalizacji oraz zaradzenie niedostatkom nauki prawa międzynarodowego publicznego nie jest możliwe bez zmiany dotychczasowego paradygmatu. Konstytutywnym elementem nowego paradygmatu powinna być antropoarchiczność, czyli postawienie osoby z jej wrodzoną i równą godnością w miejsce suwerenności państwa. Autorzy dokonują typologii cech osoby, relewantnych jako aksjologiczne uwarunkowania systemu prawnego. Wprowadzają tym samym do rozważań prawnych ujęcie interdyscyplinarne z zakresu antropologii filozoficznej. Przedstawiona koncepcja sytuuje się w kręgu non-pozytywizmu, a jej szczególna wartość wyjaśniająca odnosi się do zagadnienia celowości prawa oraz jego autonomii względem państwa