The Versatile Molecular Complex Component LC8 Promotes Several Distinct Steps of Flagellar Assembly

LC8 is present in various molecular complexes. However, its role in these complexes remains unclear. We discovered that although LC8 is a subunit of the radial spoke (RS) complex in Chlamydomonas flagella, it was undetectable in the RS precursor that is converted into the mature RS at the tip of elongating axonemes. Interestingly, LC8 dimers bound in tandem to the N-terminal region of a spoke phosphoprotein, RS protein 3 (RSP3), that docks RSs to axonemes. LC8 enhanced the binding of RSP3 N-terminal fragments to purified axonemes. Likewise, the N-terminal fragments extracted from axonemes contained LC8 and putative spoke-docking proteins. Lastly, perturbations of RSP3’s LC8-binding sites resulted in asynchronous flagella with hypophosphorylated RSP3 and defective associations between LC8, RSs, and axonemes. We propose that at the tip of flagella, an array of LC8 dimers binds to RSP3 in RS precursors, triggering phosphorylation, stalk base formation, and axoneme targeting. These multiple effects shed new light on fundamental questions about LC8-containing complexes and axoneme assembly

Exploiting Time Series Analysis in Twitter to Measure a Campaign Process Performance

© 2017 IEEE. While there are several metrics to measure business process performance, recently there is an additional requirement from businesses to evaluate business processes based on their impact on users. In this work, we evaluate business process performance using social media analytics. We view a marketing campaign as a business process and we evaluate its performance based on its impact on the Twitter. We propose a new way to calculate the \u27follow\u27 relationship in Twitter based on the users\u27 reaction to the marketing campaign process activities and we use time series and sentiment analysis for defining and measuring performance. We re-build the Twitter graph based on users\u27 reactions to the marketing activities in time and we are using community detection algorithms to identify the size of the \u27follow\u27 community and thus we define metrics to calculate the impact of the marketing/campaign process. We evaluate our approach using a dataset for a given politician. We re-construct the campaign process as a set of activities on specific topics (promotions) in time using LDA. Our results show that social media analytics can be used as a valid metric for assessing business processes performance

Time-Series Clustering for Determining Behavioral-Based Brand Loyalty of Users Across Social Media

In recent years social media data analytics allow enterprises to adopt a data driven approach to manage their processes. Although social media provide a plethora of data, still much work needs to be done to transform these data into services that businesses can use and make insightful decisions. In this work, we address one of the most important problems in business, the relationship with the customers and more precisely the identification of loyal customers. We use behavioral analytics to model and process customer actions. We extract user behavior based on our unified crawling approach and collect data from three different social media namely Reddit, Twitter and YouTube. We are extracting for each user three different behaviors namely communication, sentiment and product mix and convert them into a 3-D time-series. We use shapelet clustering to determine the loyal users. To verify our approach, we develop a set of metrics based on trust, commitment and engagement and we show that our approach results in differentiating the loyal users successfully. Moreover, we validate our results presenting a word cloud visualization. We extend our methodology introducing a semantic data transformation algorithm where we use the topic extraction, reducing the time-series to a more relevant one. Our experiments show that based on the verification metrics, our transformation increases the accuracy of the clustering results

Behavioral and Migration Analysis of the Dynamic Customer Relationships on Twitter

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. Relationship management has been of strategic importance for businesses that are interested to evaluate the state of the relationship with the customer and if possible to migrate customers to better and more binding states. This work addresses the problem of estimating the relationship state of a customer and examining the migration policy of the customer, using social media analytics. We propose an innovative framework, where clustering, linguistic and emotional analytics are used to automatically assign users to relationship states. Our research is of multi-disciplinary nature, where we are using existing results from surveys on users’ behavior when mitigating states to verify the semantics of our metrics, showing that they follow similar behavior. Our results show that clustering users based on communication, emotions and perceived product mix can result in an automated assignment of users to states. Furthermore, trust, commitment and homophily are defined and our results show that users are migrating states influenced by these values. Our work provides data analytics metrics for businesses that will identify and address the problem of relationship management thus improving the overall users’ satisfaction using a data analytics approach

The use of information and communication technologies in higher nursing education

Εισαγωγή: Οι νέες τεχνολογίες έχουν επηρεάσει καταλυτικά την καθημερινότητα των ανθρώπων σε ποικίλα πεδία. Οι Τεχνολογίες της Πληροφορίας και Επικοινωνίας (ΤΠΕ) με δεδομένο ότι αποτελούν μια από τις πέντε ικανότητες που πρέπει να διαθέτουν οι επαγγελματίες υγείας σύμφωνα με την εισηγητική έκθεση του Institute of Medicine στις Η.Π.Α., παρέχουν πλήθος δυνατοτήτων αναφορικά με την εκπαίδευση των νοσηλευτών σε θεωρητικό και κλινικό επίπεδο. Σκοπός: Σκοπός της παρούσας μελέτης ήταν να παρουσιαστεί η νέα πραγματικότητα που προσφέρουν οι ΤΠΕ στην Ανώτατη Νοσηλευτική Εκπαίδευση, μέσω των εφαρμογών που παρέχονται. Μεθοδολογία: Η μεθοδολογία περιελάμβανε συλλογή πληροφοριών σχετικά με το θέμα από άρθρα και βιβλία της τελευταίας δεκαετίας στις βάσεις δεδομένων Pubmed, Iatrotek, καθώς και στο Σύνδεσμο Ελληνικών Ακαδημαϊκών Βιβλιοθηκών (HEAL-Link). Αποτελέσματα: Από την ανασκόπηση της βιβλιογραφίας προέκυψε ότι με τη χρήση των ΤΠΕ παρέχονται ιδιαίτερες δυνατότητες στους εκπαιδευόμενους μελλοντικούς νοσηλευτές αποτελώντας σημαντικά εκπαιδευτικά εργαλεία τόσο για τους ίδιους, όσο και για τους εκπαιδευτικούς τους. Σύμφωνα με τα αποτελέσματα έρευνας στην Αμερική φάνηκε ότι το 53% των νοσηλευτικών σχολών έναντι 45% των ιατρικών χρησιμοποιούν εργαλεία όπως blogs ή wikis στα προγράμματα σπουδών τους. Παράλληλα τα μέσα κοινωνικής δικτύωσης όπως το Facebook ή το Twitter αλλά και το YouΤube χώρους αλληλεπίδρασης μεταξύ εκπαιδευόμενων και καθηγητών μιας και απαριθμούν εκατομμύρια χρήστες παγκοσμίως. Στην Ελλάδα χαρακτηριστικά το 63% των χρηστών του διαδικτύου έχει δημιουργήσει προφίλ σε κάποια υπηρεσία κοινωνικής δικτύωσης, με τον αντίστοιχο ευρωπαϊκό μέσο όρο να είναι 45%. Τα οφέλη από την χρήση των ΤΠΕ στη νοσηλευτική εκπαίδευση είναι πολλαπλά μιας και το διαδίκτυο, ο παγκόσμιος ιστός και η ποικιλία λογισμικών εικονικών κόσμων, παρέχουν τα εχέγγυα μιας μαθησιακής διαδικασίας με σύγχρονη μεθοδολογία, περιεχόμενο και στοχοθεσία που θα οδηγήσουν σε ενεργούς, κριτικά σκεπτόμενους σε πνεύμα ομαδικότητας και συνεργασίας νοσηλευτές, ευπροάρμοστους σε διαφορετικά περιβάλλοντα. Σε κάθε περίπτωση οι προβληματισμοί έναντι των ΤΠΕ στη νοσηλευτική εκπαίδευση σκιαγραφούν ζητήματα όπως το κόστος, η ποιότητα της γνώσης και η ανθρωπιστική διάσταση της διδασκαλίας. Συμπεράσματα: Οι ΤΠΕ αποτελούν αδιαμφισβήτητα μια νέα πραγματικότητα και δύνανται με ορθολογική χρήση τους να αποτελέσουν τον ακρογωνιαίο λίθο της σύγχρονης ανώτατης νοσηλευτικής εκπαίδευσης.Introduction: New technologies have catalytically influenced the everyday life of people in a variety of fields. Information and Communication Technologies (ICTs), given that they are one of the five skills that health professionals have to offer, according to the Institute of Medicine's report in the United States, provide a wealth of opportunities for training nurses at a theoretical and clinical level. Purpose: The purpose of this study was to present the new reality that ICTs offers to higher-education nursing through the applications provided. Methodology: The methodology included a collection of information on the subject from articles and books of the last decade in the Pubmed, Iatrotek databases and the Hellenic Academic Libraries Association (HEAL-Link). Results: The review of the literature has shown that using ICTs offers special opportunities for trainee future nurses as important educational tools for both themselves and their teachers. According to research results in America, 53% of nursing schools compared to 45% of medical practitioners use tools such as blogs or wikis in their curricula. At the same time, social media such as Facebook or Twitter, as well as YouTube, interact with learners and teachers as they enumerate millions of users worldwide. In Greece, 63% of Internet users have created profiles in a social networking service, with the corresponding European average being 45%. The benefits of using ICTs in nursing education are many as the Internet, the global web and the variety of virtual world software provide the guarantee of a learning process with modern methodology, content and targeting that will lead to active, critical thinkers in a team spirit and cooperative nurses, versatile in different environments. In any case, ICTs concerns in nursing education outline issues such as cost, quality of knowledge and the humanitarian dimension of teaching. Conclusion: ICTs is undoubtedly a new reality and can be a cornerstone of modern higher educatio

Nursing and sustainable development

Μολονότι οι ερευνητές στον τομέα της νοσηλευτικής έχουν τονίσει το ζήτημα της βιωσιμότητας στο χώρο της υγείας, το πεδίο και οι διαστάσεις της αειφορίας στη νοσηλευτική είναι απροσδιόριστες και ανεπαρκείς. Η βιωσιμότητα στη νοσηλευτική περιλαμβάνει τις έννοιες: οικολογία, περιβάλλον, μέλλον, παγκοσμιοποίηση, ολισμός και συντήρηση. Οι νοσηλευτές στα πλαίσια της βιωσιμότητας πρέπει να διαθέτουν γνώσεις επί της κλιματικής αλλαγής και των περιβαλλοντικών επιπτώσεων, να ευαισθητοποιηθούν και να εμφανίζουν υπευθυνότητα προθυμία να αλλάξει η στόχευση της νοσηλευτικής επιστήμης προς την αειφορία. Αυτό αφορά τόσο την άσκηση της νοσηλευτικής κλινικής πρακτικής σε συνδυασμό με κατάλληλες αναπροσαρμογές και κατευθύνσεις της νοσηλευτικής εκπαίδευσης κι εφαρμογής προγραμμάτων αγωγής υγείας στην κοινότητα από επαγγελματίες νοσηλευτές. Η νοσηλευτική δύναται να συμβάλλει στην αειφόρο ανάπτυξη, με τελικό στόχο τη διατήρηση ενός περιβάλλοντος που δεν παρακωλύει τις ευκαιρίες των σημερινών και μελλοντικών γενεών για βέλτιστο επίπεδο υγείας. Η μελλοντική νοσηλευτική έρευνα πρέπει να ενσωματώσει τις αρχές και συστάσεις περί βιωσιμότητας στο σύχρονο κόσμο στα πλαίσια ενός αειφορικού κι ολιστικού μοντέλου υγειονομικής περίθαλψης.Introduction: Although nursing researchers have highlighted the issue of sustainability in the health sector, the scope and dimensions of sustainability in nursing are unspecified and inadequate. Purpose: The purpose of this study was to describe the meaning and dimensions of sustainability in nursing science, practice and education and to explore the role of nurses as members of the global community. Methodology: The methodology included a collection of information on the subject from articles and books of the last decade in the PubMed, Cinahl databases and the Hellenic Academic Libraries (HEAL-Link) link, as well as in research articles, books, scientific journals and websites. Results: Sustainability in nursing includes concepts: ecology, environment, future, globalization, population and maintenance. Sustainability nurses must have knowledge of climate change and environmental impact, be sensitized and responsibly willing to change the goal of nursing science towards sustainability. This applies both to the practice of nursing clinical practice in conjunction with appropriate adaptations and directions of nursing education and the implementation of health education programs in the community by professional nurses. Conclusion: Nursing can contribute to sustainable development with the ultimate goal of maintaining an environment that does not hamper the opportunities of present and future generations for optimal health. Future nursing research should incorporate the principles and recommendations of sustainability into the modern world in the context of a sustainable and holistic healthcare model

Μηχανισμοί Ανοικείωσης στη Λάμψη του ΣτάνλεΪ Κιούμπρικ

Στην εργασία επιχειρείται η μελέτη και ανάλυση των κινηματογραφικών μηχανισμών ανοικείωσης που χρησιμοποιούνται στην ταινία "Η Λάμψη" του Στάνλεϊ Κιούμπρικ. Αναδεικνύεται πως ζητήματα όπως ο ήχος, το παρελθόν ενός χώρου, η ακούσια επανάληψη προσώπων, εκφράσεων και καταστάσεων, η κατάλυση των ορίων πραγματικότητας και φαντασίας, η ασυνήθιστη εικόνα του ανθρώπινου σώματος και το μοτίβο του σωσία χρησιμοποιούνται, προκειμένου να προκαλέσουν την αίσθηση του ανοίκειου.The thesis attempts to study and analyze the cinematic mechanisms οf uncanniness used in the film "The Shining" by Stanley Kubrick. The thesis analyses how how issues such as sound, the past of a space, the involuntary repetition of faces, expressions and situations, the dissolution of the boundaries between reality and fantasy, the unusual image of the human body and the pattern of the body double are used to evoke a sense of the uncanny

Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration

Tissue development and regeneration depend on cell-cell interactions and signals that target stem cells and their immediate progeny. However, the cellular behaviours that lead to a properly regenerated tissue are not well understood. Using a new, non-invasive, intravital two-photon imaging approach we study physiological hair-follicle regeneration over time in live mice. By these means we have monitored the behaviour of epithelial stem cells and their progeny during physiological hair regeneration and addressed how the mesenchyme influences their behaviour. Consistent with earlier studies, stem cells are quiescent during the initial stages of hair regeneration, whereas the progeny are more actively dividing. Moreover, stem cell progeny divisions are spatially organized within follicles. In addition to cell divisions, coordinated cell movements of the progeny allow the rapid expansion of the hair follicle. Finally, we show the requirement of the mesenchyme for hair regeneration through targeted cell ablation and long-term tracking of live hair follicles. Thus, we have established an in vivo approach that has led to the direct observation of cellular mechanisms of growth regulation within the hair follicle and that has enabled us to precisely investigate functional requirements of hair-follicle components during the process of physiological regeneration. © 2012 Macmillan Publishers Limited. All rights reserved

Verbal violence in the professional life of nurses

Εισαγωγή: Η λεκτική βία αποτελεί ένα συχνό κοινωνικό φαινόμενο αναφερόμενο και σε εργασιακούς χώρους άσκησης της νοσηλευτικής επιστήμης.Σκοπός: Η διερεύνηση του φαινομένου της λεκτικής βίας στην επαγγελματική ζωή των νοσηλευτών.Υλικό και Mέθοδος: Το δείγμα (Ν=168) αποτέλεσαν εργαζόμενοι νοσηλευτές σε κλινικές, μονάδες φροντίδας και κέντρα υγείας δημοσίου και ιδιωτικού τομέα, ενώ ερευνητικό εργαλείο αποτέλεσε η Eλληνική εκδοχή του ερωτηματολογίου Verbal Abuse Scale (VAS-GR).Αποτελέσματα: Το 97,6% των νοσηλευτών ανεξαρτήτου ηλικίας έχει υπάρξει θύμα ή θεατής λεκτικής βίας. Το 28% του δείγματος ανέφερε πως η προσβολή συγκαλυμμένη με αστείο εμφανίζεται αρκετά συχνά, ενώ το 29% πως η έντονη κριτική και η επίρριψη ευθυνών και κατηγοριών εμφανίζονται πολύ συχνά. Η σχέση με τον υβριστή ποικίλει με τον άμεσο προϊστάμενο να έχει συγκεντρώσει το μεγαλύτερο ποσοστό. Το 26,8% ανέφερε ότι το φαινόμενο εμφανίζεται περισσότερες από μία φορά ετησίως, το 52% θεώρησε το γεγονός αρκετά έως πολύ αγχωτικό, ενώ η μεγάλη πλειοψηφία δήλωσε αρκετό ή πάρα πολύ θυμό. Πλησίον του 50% ως αποδεκτό τρόπο προσέγγισης ανεφέρεται η διευθέτηση της πιθανής παρανόησης και κύρια μακροπρόθεσμη συνέπεια η μη ικανοποίηση από την εργασία. Επίσης οι γυναίκες έτειναν να κατηγορούν τον εαυτό τους περισσότερο από τους άνδρες. Τέλος ο συντελεστής εσωτερικής συνέπειας ερωτηματολογίων Cronbach’s alpha ήταν 0.957.Συμπεράσματα: Ο εργαζόμενος νοσηλευτής αντιμετωπίζει συχνά επεισόδια λεκτικής βίας με επιπτώσεις στην ψυχική του υγεία και στην βιωσιμότητά του στον εργασιακό χώρο. Αναδύεται λοιπόν ως επιτακτική η ανάγκη για έγκαιρη αναγνώριση των παραγόντων που αφορούν στην εκδήλωση της βίαιης λεκτικής συμπεριφοράς στους χώρους εργασίας των νοσηλευτών και στην αποτελεσματική αντιμετώπισή του.Introduction: Verbal violence is a common social phenomenon that is also observed in nursing science workplaces. Aim: This research project was aimed at investigating the phenomenon of verbal violence in the professional life of nursing staff. Material and Method: The sample (N=168) consisted of nurses working in clinics, care units, health centers in both the public and private sectors. The data collection was done using the Greek version of Verbal Abuse Scale questionnaire (VAS-GR). Results: The survey revealed that at a rate of 97.6%, nursing staff of all ages has been victim of verbal violence. 28% of the sample thinks the insane jesting appears fairly often, while 29% says that intense criticism and accusations of accusations and accusations often appear as forms of verbal violence. The relationship with the abuser seems to vary with the immediate superior having accumulated the highest percentage of 21.4%.Regarding the frequency of the phenomenon, much of the sample (26.8%) replied that the frequency rate reaches up to more than once a year. 52% considers the episode of verbal violence from fairly to very stressful, while the vast majority of the sample indicates enough or too much anger. More than 50% of the respondents consider an acceptable approach to addressing possible victimization, focusing as the main long-term consequence of non-satisfaction. Young age groups have found controversial occupations to deal with stress, while women tend to blame themselves more than men. Finally, it should be noted that the sample reliability test was very high (Cronbach's alpha index = 0.957), indicating that the results of the survey can be generalized. Conclusions: The nurse in his working environment is often confronted with episodes of verbal violence, mainly affecting his mental health and hence his sustainability in the workplace. The urgency of identifying the factors involved in creating the phenomenon of violent verbal behavior in the workplace of the nurses and in its effective treatment is therefore urgently needed

A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells.

BACKGROUND: Pax7 is a transcription factor involved in the specification and maintenance of muscle stem cells (MuSCs). Upon injury, MuSCs leave their quiescent state, downregulate Pax7 and differentiate, contributing to skeletal muscle regeneration. In the majority of regeneration studies, MuSCs are isolated by fluorescence-activated sorting (FACS), based on cell surface markers. It is known that MuSCs are a heterogeneous population and only a small percentage of isolated cells are true stem cells that are able to self-renew. A strong Pax7 reporter line would be valuable to study the in vivo behavior of Pax7-expressing stem cells. METHODS: We generated and characterized the muscle properties of a new transgenic Pax7EGFP mouse. Utilizing traditional immunofluorescence assays, we analyzed whole embryos and muscle sections by fluorescence microscopy, in addition to whole skeletal muscles by 2-photon microscopy, to detect the specificity of EGFP expression. Skeletal muscles from Pax7EGFP mice were also evaluated in steady state and under injury conditions. Finally, MuSCs-derived from Pax7EGFP and control mice were sorted and analyzed by FACS and their myogenic activity was comparatively examined. RESULTS: Our studies provide a new Pax7 reporter line with robust EGFP expression, detectable by both flow cytometry and fluorescence microscopy. Pax7EGFP-derived MuSCs have identical properties to that of wild-type MuSCs, both in vitro and in vivo, excluding any positional effect due to the transgene insertion. Furthermore, we demonstrated high specificity of EGFP to label MuSCs in a temporal manner that recapitulates the reported Pax7 expression pattern. Interestingly, immunofluorescence analysis showed that the robust expression of EGFP marks cells in the satellite cell position of adult muscles in fixed and live tissues. CONCLUSIONS: This mouse could be an invaluable tool for the study of a variety of questions related to MuSC biology, including but not limited to population heterogeneity, polarity, aging, regeneration, and motility, either by itself or in combination with mice harboring additional genetic alterations