A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications.

RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative analysis of messenger RNA molecules in a biological sample and is useful for studying cellular responses. RNA-seq has fueled much discovery and innovation in medicine over recent years. For practical reasons, the technique is usually conducted on samples comprising thousands to millions of cells. However, this has hindered direct assessment of the fundamental unit of biology-the cell. Since the first single-cell RNA-sequencing (scRNA-seq) study was published in 2009, many more have been conducted, mostly by specialist laboratories with unique skills in wet-lab single-cell genomics, bioinformatics, and computation. However, with the increasing commercial availability of scRNA-seq platforms, and the rapid ongoing maturation of bioinformatics approaches, a point has been reached where any biomedical researcher or clinician can use scRNA-seq to make exciting discoveries. In this review, we present a practical guide to help researchers design their first scRNA-seq studies, including introductory information on experimental hardware, protocol choice, quality control, data analysis and biological interpretation

Tower-Top Antenna Array Calibration Scheme for Next Generation Networks

Recently, there has been increased interest in moving the RF electronics in basestations from the bottom of the tower to the top, yielding improved power efficiencies and reductions in infrastructural costs. Tower-top systems have faced resistance in the past due to such issues as increased weight, size, and poor potential reliability. However, modern advances in reducing the size and complexity of RF subsystems have made the tower-top model more viable. Tower-top relocation, however, faces many significant engineering challenges. Two such challenges are the calibration of the tower-top array and ensuring adequate reliability. We present a tower-top smart antenna calibration scheme designed for high-reliability tower-top operation. Our calibration scheme is based upon an array of coupled reference elements which sense the array's output. We outline the theoretical limits of the accuracy of this calibration, using simple feedback-based calibration algorithms, and present their predicted performance based on initial prototyping of a precision coupler circuit for a 2×2 array. As the basis for future study a more sophisticated algorithm for array calibration is also presented whose performance improves with array size