Detection of Azuki Bean Wild Relatives Having Different Salt Tolerance Mechanism and Construction of Molecular Linkage Map of Tuber Cowpea

三重大学博士(学術)application/pdf要約 / 三重大学大学院生物資源学研究科 資源循環学専攻thesi

Detection of Azuki Bean Wild Relatives Having Different Salt Tolerance Mechanism and Construction of Molecular Linkage Map of Tuber Cowpea

三重大学博士（学術）application/pdf本文 / Laboratory of Crop Production and Ecology, Major of Sustainable Resource Sciences, Graduate School of Bioresources, Mie University 95pdoctoral thesi

Detection of Azuki Bean Wild Relatives Having Different Salt Tolerance Mechanism and Construction of Molecular Linkage Map of Tuber Cowpea

三重大学博士(学術)application/pdf内容の要旨・審査結果の要旨 / 資源循環学thesi

Detection of Azuki Bean Wild Relatives Having Different Salt Tolerance Mechanism and Construction of Molecular Linkage Map of Tuber Cowpea

内容の要旨・審査結果の要旨 / 資源循環学博士(学術)三重大

A rapid quantification method for tissue Na+ and K+ concentrations in salt-tolerant and susceptible accessions in Vigna vexillata (L.) A. Rich.

A rapid quantification method for leaf sodium (Na+) and potassium (K+) concentrations was developed using a compact ion meter. Leaf ion concentrations were evaluated for species of Vigna vexillata (L.) A. Rich. after two weeks of treatment with 0–200 mM of sodium chloride. To compare the ion extraction efficiency, extraction solutions of distilled water and ammonium acetate were tested. The ion concentrations of extracts obtained by both solutions were measured using an ion meter, and the values were validated using ion chromatography. For both extraction solutions, the ion meter values were highly correlated with those of the ion chromatograph. However, correlations between ion meter and chromatograph values were largely different for Na+ and K+. The rapid quantification of ion concentrations using an ion meter developed in this study was successfully utilized for evaluating differences in leaf Na+ concentrations, K+ concentrations, and K+/Na+ ratio in species of V. vexillata

Developing Guidelines for Azolla microphylla Production as Compost for Sustainable Agriculture

Azolla is a substitute compost that has the potential to enhance nutrient cycling in agricultural systems for sustainable development. In this study, four experiments were conducted to compare the Department of Agriculture (DOA, Thailand)’s methodology for determining the suitable type and rate of animal manure and the optimal light intensity for the growth and yield of Azolla (Azolla microphylla). The results revealed that applying 100% pig manure gave the highest yield of Azolla compared to the other manures. However, there was no discernible (p > 0.05) difference in yield across the various doses (20.16, 30.16, and 40.16 gN m−2) of pig manure treatments, for which the minimal pig manure dosage of 20.16 gN m−2 was chosen. For further experimentation in the optimal light intensity, the 40% shading gave the highest yield of Azolla compared to no shading or 20 and 60% shading (p ≤ 0.01). When compared with the DOA Thailand methodology (1.27 kg m−2 of cow manure and covered with a size 32 mesh net), the findings indicated that the modified method (20.16 gN m−2 of pig manure + 40% shading) gave a 16% greater Azolla yield than that under the DOA Thailand methodology. The current finding method can produce a monthly fresh biomass of A. microphylla of 40.7 t ha−1 year−1 with higher contents of total N (4.92%) and lower C:N ratio (≤10:1) that could release minerals relatively rapidly. Its use can be encouraged by farmers to produce their own ecofriendly biofertilizer or soil amendment for sustainable agriculture

Practical Guidelines for Farm Waste Utilization in Sustainable Kale Production

Natural amendments from agricultural waste to improve soil physicochemical properties continuously attract research interest in promoting eco-friendly plant production. The present study evaluated the proper use of sawdust, biochar, and compost made from farm waste for kale production from seedling propagation to field conditions. From the seedling propagation process, the results demonstrate that the most suitable growing medium for kale seedings was 0.5:1:1 v/v of sawdust + biochar + compost, which gave the fastest mean germination times (2.71 days) and the highest seed germination percentage (78.33%). In addition to investigating the selected growing media as the soil amendments at five different rates (0, 6.25, 12.50, 18.75, 25.00, and 31.25 t ha−1), the result reveals that the fresh weight of marketable leaves was significantly highest under the 31.25 t ha−1 treatment. The application rate that yielded the highest gross profit margins was eight times higher than the control. Moreover, in some harvesting periods, the kale leaf yields under the treatment of 31.25 t ha−1 showed higher total chlorophyll and carotenoid contents