Correlation of qEEG with PET in schizophrenia

PET relative metabolism was correlated with quantitative EEG in 9 schizophrenic patients. The PET metabolic regions of interest were the frontal lobes, thalamus and basal ganglia, and right and left temporal lobes. Significant positive correlations were seen for the frontal lobes and delta EEG power, and alpha power with subcortical metabolism. The physiologic plausibility of those correlations is discussed with reference to the possible effect of neuroleptic medication

A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae

All authors are with the Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, USA -- Hal S. Alper is with the Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, USA -- Amanda M. Lanza Current Address: Bristol-Myers Squibb, Biologics Development, 35 South Street, Hopkinton, MA 01748, USABackground: Heterologous gene expression is an important tool for synthetic biology that enables metabolic engineering and the production of non-natural biologics in a variety of host organisms. The translational efficiency of heterologous genes can often be improved by optimizing synonymous codon usage to better match the host organism. However, traditional approaches for optimization neglect to take into account many factors known to influence synonymous codon distributions. Results: Here we define an alternative approach for codon optimization that utilizes systems level information and codon context for the condition under which heterologous genes are being expressed. Furthermore, we utilize a probabilistic algorithm to generate multiple variants of a given gene. We demonstrate improved translational efficiency using this condition-specific codon optimization approach with two heterologous genes, the fluorescent protein-encoding eGFP and the catechol 1,2-dioxygenase gene CatA, expressed in S. cerevisiae. For the latter case, optimization for stationary phase production resulted in nearly 2.9-fold improvements over commercial gene optimization algorithms. Conclusions: Codon optimization is now often a standard tool for protein expression, and while a variety of tools and approaches have been developed, they do not guarantee improved performance for all hosts of applications. Here, we suggest an alternative method for condition-specific codon optimization and demonstrate its utility in Saccharomyces cerevisiae as a proof of concept. However, this technique should be applicable to any organism for which gene expression data can be generated and is thus of potential interest for a variety of applications in metabolic and cellular engineering.Chemical EngineeringInstitute for Cellular and Molecular [email protected]

An Authorisation Scenario for S-OGSA

The Semantic Grid initiative aims to exploit knowledge in the Grid to increase the automation, interoperability and flexibility of Grid middleware and applications. To bring a principled approach to developing Semantic Grid Systems, and to outline their core capabilities and behaviors, we have devised a reference Semantic Grid Architecture called S-OGSA. We present the implementation of an S-OGSA observant semantically-enabled Grid authorization scenario, which demonstrates two aspects: 1) the roles of different middleware components, be them semantic or non-semantic, and 2) the utility of explicit semantics for undertaking an essential activity in the Grid: resource access control

S-OGSA as a Reference Architecture for OntoGrid and for the Semantic Grid

The Grid aims to support secure, flexible and coordinated resource sharing through providing a middleware platform for advanced distributing computing. Consequently, the Grid’s infrastructural machinery aims to allow collections of any kind of resources—computing, storage, data sets, digital libraries, scientific instruments, people, etc—to easily form Virtual Organisations (VOs) that cross organisational boundaries in order to work together to solve a problem. A Grid depends on understanding the available resources, their capabilities, how to assemble them and how to best exploit them. Thus Grid middleware and the Grid applications they support thrive on the metadata that describes resources in all their forms, the VOs, the policies that drive then and so on, together with the knowledge to apply that metadata intelligently

Managing semantic Grid metadata in S-OGSA

Grid resources such as data, services, and equipment, are increasingly being annotated with descriptive metadata that facilitates their discovery and their use in the context of Virtual Organizations (VO). Making such growing body of metadata explicit and available to Grid services is key to the success of the VO paradigm. In this paper we present a model and management architecture for Semantic Bindings, i.e., firstclass Grid entities that encapsulate metadata on the Grid and make it available through predictable access patterns. The model is at the core of the S-OGSA reference architecture for the Semantic Grid

Octilinear Force-Directed Layout with Mental Map Preservation for Schematic Diagrams

We present an algorithm for automatically laying out metro map style schematics using a force-directed approach, where we use a localized version of the standard spring embedder forces combined with an octilinear magnetic force. The two types of forces used during layout are naturally conflicting, and the existing method of simply combining these to generate a resultant force does not give satisfactory results. Hence we vary the forces, emphasizing the standard forces in the beginning to produce a well distributed graph, with the octilinear forces becoming prevalent at the end of the layout, to ensure that the key requirement of line angles at intervals of 45? is obtained. Our method is considerably faster than the more commonly used search-based approaches, and we believe the results are superior to the previous force-directed approach. We have further developed this technique to address the issues of dynamic schematic layout. We use a Delaunay triangulation to construct a schematic “frame”, which is used to retain relative node positions and permits full control of the level of mental map preservation. This technique is the first to combine mental map preservation techniques with the additional layout criteria of schematic diagrams. To conclude, we present the results of a study to investigate the relationship between the level of mental map preservation and the user response time and accuracy