A non-contact optical technique for vehicle tracking along bounded trajectories

This paper presents a method for measuring the non-controlled trajectory of a cart along a bounded rectilinear path. The method uses non-contact measurement devices to identify the position of a movable laser scanner working in helical mode in order to reconstruct the 3D model of bridges. The main idea of the proposed method is to use vision systems in order to identify the coordinates of the laser scanner placed on the cart with respect to the global reference system. A fit-to-purpose vision system has been implemented: the system uses three CCD's cameras mounted on the cart to identify the relative rotations with respect to the environment. Two lasers pointers and a laser distance meter are fixed at the starting point of the trajectory and pointing in the direction of motion of the cart, creating three dots on a plane placed on the cart. One of the camera detects the cart displacements and rotations in the plane using a blob analysis procedure. The method described in this paper has a constant uncertainty and the measurement range only depends on the lasers power. The theoretical accuracy of the measurement system is close to 1 mm for the translation along the motion direction and around 0.5 mm along the other two directions. Orientations measurement have a theoretical accuracy of less than 0.1 °. The solution has been implemented for the 3D reconstruction of concrete bridge; preliminary experimental results are presented and discussed

Vehicle sideslip estimation for four-wheel-steering vehicles using a particle filter

The availability of the most relevant vehicle states is crucial for the development of advanced vehicle control systems and driver assistance systems. Specifically the vehicle sideslip angle plays a key role, yet this state is unpractical to measure and still not straightforward to estimate. This paper investigates a particle filter approach to estimate the chassis sideslip angle of road vehicles. The filter relies on a physical model of the vehicle and on measurements available from cheap and widespread sensors including inertial measurement unit and steering wheel angle sensor(s). The approach is validated using experimental data collected with the research platform RoboMobil (RoMo), a by-wire electric vehicle with wheel-individual traction and steering actuators. Results show that the performance of the proposed particle filter is satisfactory, and indicate directions for further improvement

Scenario Generation and Autonomous Control for High-Precision Vineyard Operations

Precision Farming (PF) in vineyards represents an innovative approach to vine cultivation that leverages the advantages of the latest technologies to optimize resource use and improve overall field management. This study investigates the application of PF techniques in a vineyard, focusing on sensor-based decision-making for autonomous driving. The goal of this research is to define a repeatable methodology for virtual testing of autonomous driving operations in a vineyard, considering realistic scenarios, efficient control architectures, and reliable sensors. The simulation scenario was created to replicate the conditions of a real vineyard, including elevation, banking profiles, and vine positioning. This provides a safe environment for training operators and testing tools such as sensors, algorithms, or controllers. This study also proposes an efficient control scheme, implemented as a state machine, to autonomously drive the tractor during two distinct phases of the navigation process: between rows and out of the field. The implementation demonstrates improvements in trajectory-following precision while reducing the intervention required by the farmer. The proposed system was extensively tested in a virtual environment, with a particular focus on evaluating the effects of micro and macro terrain irregularities on the results. A key feature of the control framework is its ability to achieve adequate accuracy while minimizing the number of sensors used, relying on a configuration of a Global Navigation Satellite System (GNSS) and an Inertial Measurement Unit (IMU) as a cost-effective solution. This minimal-sensor approach, which includes a state machine designed to seamlessly transition between in-field and out-of-field operations, balances performance and cost efficiency. The system was validated through a wide range of simulations, highlighting its robustness and adaptability to various terrain conditions. The main contributions of this work include the high fidelity of the simulation scenario, the efficient integration of the control algorithm and sensors for the two navigation phases, and the detailed analysis of terrain conditions. Together, these elements form a robust framework for testing autonomous tractor operations in vineyards

Effects of putrescine, cadaverine, spermine, spermidine and β-phenylethylamine on cultured bovine mammary epithelial cells

A bovine mammary epithelial cell line (BME-UV1) and three-dimensional collagen primary bovine organoids were used to evaluate the effects of cadaverine, putrescine, spermine, spermidine and β-phenylethylamine on mammary epithelial cells. Each biogenic amine was diluted in several concentrations (0-50 mM in BME-UV1 and 0-4 mM in primary bovine organoids) in the appropriate saline solution for the cell culture considered. In order to determine the activity of each compound tritiated thymidine incorporation was used. At low concentrations, all amines induced cell proliferation in both cultures. In BME-UV1, spermine significantly inhibited cell proliferation (P<0.001), while the other amines inhibited at higher concentrations (50mM). In primary bovine organoids, β−phenylethylamine significantly (P<0.001) inhibited cell proliferation at 4 mM. Organoids cultured in the presence of all amines, except β-phenylethylamine, had stellate projections indicating intense cell proliferation. Proliferation of mammary epithelial cells was stimulated at low concentrations, while at high concentrations it was inhibited. Our results suggested that the effects of each compound on mammary epithelial cells could be related to the compound itself and not to mediating by the bovine amino oxidase, responsible of the formation of toxic metabolites

In linezolid underexposure, pharmacogenetics matters: The role of CYP3A5

The exposure to linezolid is characterized by a large inter-individual variability; age, renal dysfunction and body weight explain this variability only to a limited extent and a considerable portion of it remains unexplained; therefore, we decided to investigate the role of individual genetic background focusing in particular on the risk of linezolid underexposure. 191 patients in therapy with linezolid at the standard dose of 600 mg twice daily were considered. Linezolid plasma concentration was determined at the steady state and classified as “below”, “within” or “above” reference range. Genetic polymorphisms for ATP Binding Cassette Subfamily B Member 1 (ABCB1), Cytochrome P450 (CYP) enzymes CYP3A4 and CYP3A5, and Cytochrome P450 Oxidoreductase (POR) were investigated. Age significantly correlated with drug exposure, and patients CYP3A5 expressers (GA and AA) were found at high risk to be underexposed to the drug when treated at standard dose. This association was confirmed even after correction with age. No association was found with ABCB1 polymorphism. Our data suggest that CYP3A5 polymorphisms might significantly affect linezolid disposition, putting patients at higher risk to be underexposed, while P-glycoprotein polymorphism seem not to play any role

Milk whey as a sustainable alternative growth supplement to fetal bovine serum in muscle cell culture

In vitro meat cultivation, a cutting-edge innovation in food science, may represent a more sustainable and ethical source of animal proteins compared with conventionally grown meat. An important challenge for meat cultivation lies in eliminating the use of fetal bovine serum (FBS) in cell culture media due to ethical concerns. Milk whey is a nutrient-rich liquid portion of the milk, derived as a byproduct of dairy industry. Similar to FBS, whey contains proteins that are crucial for nutrition, cell adhesion, and biomolecular transport. In this study, we investigated whether whey proteins (WP) can replace FBS in supporting muscle cell cultivation, using the C2C12 myoblast model. Accordingly, under serum-free conditions, cells were treated with 2 WP mixtures, grouping high (β-LG 1.25%, α-LA 1.25%, BSA 1.25%) and low (β-LG 0.07%, α-LA 0.15%, BSA 0.15%) selected concentrations of individual proteins that positively affected cell growth in a preceding dose-response study. Cells cultured in only basal Dulbecco's Modified Eagle Medium were included as a negative control, and cells cultured in 10% FBS as a positive control. Cells were maintained in the treatment media for 48 h (d 1 and 2) to support myoblast proliferation. Subsequently, all the treatments were replaced with a standard low mitogenic 2% horse serum (HS) medium until full differentiation (d 6). The treatment effects on morphology, viability, and lactate dehydrogenase release were assessed after d 1, 2, and 6, respectively. The results showed that WP stimulated cell proliferation under serum-free culture conditions, similar to the FBS control, and subsequently facilitated myotube formation when the WP or FBS treatments were switched to HS medium. After differentiation, these cells also exhibited increased expression of cell differentiation markers such as creatine kinase and citrate synthase and underwent morphological changes from spindle-shaped cells to fused elongated myotubes, in contrast to the negative control. This study demonstrates that WP are a promising and sustainable alternative for considerably replacing FBS-based growth supplements for use in cultivated animal products

Metacognition, Social Cognition, and Mentalizing In Psychosis: Are These Distinct Constructs When It Comes To Subjective Experience Or Are We Just Splitting Hairs?

Research using the integrated model of metacognition has suggested that the construct of metacognition could quantify the spectrum of activities that, if impaired, might cause many of the subjective disturbances found in psychosis. Research on social cognition and mentalizing in psychosis, however, has also pointed to underlying deficits in how persons make sense of their experience of themselves and others. To explore the question of whether metacognitive research in psychosis offers unique insight in the midst of these other two emerging fields, we have offered a review of the constructs and research from each field. Following that summary, we discuss ways in which research on metacognition may be distinguished from research on social cognition and mentalizing in three broad categories: (1) experimental procedures, (2) theoretical advances, and (3) clinical applications or indicated interventions. In terms of its research methods, we will describe how metacognition makes a unique contribution to understanding disturbances in how persons make sense of and interpret their own experiences within the flow of life. We will next discuss how metacognitive research in psychosis uniquely describes an architecture which when compromised – as often occurs in psychosis – results in the loss of persons’ sense of purpose, possibilities, place in the world and cohesiveness of self. Turning to clinical issues, we explore how metacognitive research offers an operational model of the architecture which if repaired or restored should promote the recovery of a coherent sense of self and others in psychosis. Finally, we discuss the concrete implications of this for recovery-oriented treatment for psychosis as well as the need for further research on the commonalities of these approaches

EFFICACIA DELLA SOMMINISTRAZIONE DI BENZOATO DI SODIO NELLE DIETE PER SUINETTI IN SVEZZAMENTO EFFICACY OF SODIUM BENZOATE SUPPLEMENTATION IN WEANING PIGLETS DIET

Gli acidi organici e i loro sali vengono da molti anni utilizzati nell\u2019alimentazione del suinetto al fine di ridurre la capacit\ue0 tampone delle diete starter. L\u2019acidificazione della dieta pu\uf2 essere considerata una misura profilattica simile a quella effettuata con gli antibiotici: mentre gli antibiotici sono prodotti per inibire la crescita batterica in generale, il processo di acidificazione indotto dall\u2019utilizzo dell\u2019acido organico permette la sopravvivenza dei microrganismi pi\uf9 adatti a colonizzare il tratto gastrointestinale (1). In relazione alla sua attivit\ue0 antibatterica e antifungina, il Na benzoato viene attualmente utilizzato come conservante di vari alimenti (2), mentre non esistono riferimenti relativi ad un suo impiego come regolatore di acidit\ue0 nelle diete per suinetti. E\u2019 stato dimostrato che l\u2019acido benzoico riduce la crescita di microrganismi potenzialmente dannosi dell\u2019intestino del suinetto (3) e riduce il pH urinario con una riduzione dell\u2019escrezione di ammoniaca (4). Sulla base di tali considerazioni \ue8 stata condotta una ricerca allo scopo di verificare gli effetti dell\u2019integrazione della dieta con sodio benzoato sulle prestazioni produttive, sulle popolazioni batteriche intestinali e sull\u2019urea plasmatica dei suinetti in post-svezzamento

Nanotechnology and its applications in food and animal science

Nanotechnology has the potential to manipulate matter at the nanometre scale, creating and assembling substances at a molecular level with new and interesting properties. This has offered opportunities for applications in different sectors. Recently, nanotechnology has received particular attention due to its promising applications in animal nutrition, drugs and nutrients delivery, animal reproduction, disease diagnosis and treatment. In the food sector, nanotechnology is applied to the improvement of food packaging, processing, monitoring and the development of food with new functional properties that can respond to the needs of consumers. This review will focus on the advances of nanotechnology in food sector and animal science with particular attention to animal nutrition. The implications for safety and regulation will be also discussed