An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

<p>Abstract</p> <p>Background</p> <p>Little is known about the composition and function of the saliva in black flies such as <it>Simulium guianense</it>, the main vector of river blindness disease in Brazil. The complex salivary potion of hematophagous arthropods counteracts their host's hemostasis, inflammation, and immunity.</p> <p>Results</p> <p>Transcriptome analysis revealed ubiquitous salivary protein families--such as the Antigen-5, Yellow, Kunitz domain, and serine proteases--in the <it>S. guianense </it>sialotranscriptome. Insect-specific families were also found. About 63.4% of all secreted products revealed protein families found only in <it>Simulium</it>. Additionally, we found a novel peptide similar to kunitoxin with a structure distantly related to serine protease inhibitors. This study revealed a relative increase of transcripts of the SVEP protein family when compared with <it>Simulium vittatum </it>and <it>S. nigrimanum </it>sialotranscriptomes. We were able to extract coding sequences from 164 proteins associated with blood and sugar feeding, the majority of which were confirmed by proteome analysis.</p> <p>Conclusions</p> <p>Our results contribute to understanding the role of <it>Simulium </it>saliva in transmission of <it>Onchocerca volvulus </it>and evolution of salivary proteins in black flies. It also consists of a platform for mining novel anti-hemostatic compounds, vaccine candidates against filariasis, and immuno-epidemiologic markers of vector exposure.</p

Notas sobre tabánidos argentinos (Dipt. insecta) : III. Los tabánidos de Patagonia y Tierra del Fuego.

Se hace una lista de las especies de tábanos patagónicos, figurando en primer término el material del Departamento de Entomología Sanitaria del Instituto Nacional de Microbiología, agregando como novedades una especie nueva para la ciencia —Dasybasis chubutensis—, una especie no citada anteriormente para el país —Dasybasis maculiuceps Kroeber— y varias localidades nuevas que amplían notablemente el área de distribución de varias especies

Notas sobre tabánidos argentinos (dipt. insecta) : II. Sobre dos especies nuevas de Dasybasis halladas en la zona de Lago Fontana.

Se describen dos especies nuevas, Dasybasis fontanensis y Dasybasis cume- lafquen basadas en hembras coleccionadas en Lago Fontana (Chubut) , con cebo humano. Se hace la descripción de la morfología externa v de la genitalia» acompañando figuras de las mismas

Notas sobre tabánidos argentinos (dipt. insecta) : II. Sobre dos especies nuevas de Dasybasis halladas en la zona de Lago Fontana.

Se describen dos especies nuevas, Dasybasis fontanensis y Dasybasis cume- lafquen basadas en hembras coleccionadas en Lago Fontana (Chubut) , con cebo humano. Se hace la descripción de la morfología externa v de la genitalia» acompañando figuras de las mismas

Notas sobre tabánidos argentinos (Dipt. insecta) : III. Los tabánidos de Patagonia y Tierra del Fuego.

Se hace una lista de las especies de tábanos patagónicos, figurando en primer término el material del Departamento de Entomología Sanitaria del Instituto Nacional de Microbiología, agregando como novedades una especie nueva para la ciencia —Dasybasis chubutensis—, una especie no citada anteriormente para el país —Dasybasis maculiuceps Kroeber— y varias localidades nuevas que amplían notablemente el área de distribución de varias especies

The immature stages of Myiotabanus barrettoi fairchild (tabanidae-diptera-insecta)

Larva and pupa of Myiotabanus barrettoi living between leaves of Pistia stratiotes in ponds of Formosa Province (Argentina) are described. As immature stages of Lepiselaga crassipes inhabit the same environment and have very a similar appearance, new information on ornamentation and morphology is added to differentiate both species. Larvae and pupae were maintained individually in moist vials at laboratory temperature until adults emerged

The immature stages of Myiotabanus barrettoi fairchild (tabanidae-diptera-insecta)

Larva and pupa of Myiotabanus barrettoi living between leaves of Pistia stratiotes in ponds of Formosa Province (Argentina) are described. As immature stages of Lepiselaga crassipes inhabit the same environment and have very a similar appearance, new information on ornamentation and morphology is added to differentiate both species. Larvae and pupae were maintained individually in moist vials at laboratory temperature until adults emerged