Analysis of genetic relationships in cashew varieties using morphological characters and ISSR markers

In the present paper genetic relationships of twenty five varieties of cashew are described on the basis of morphological characters and ISSR markers. Results obtained for the phenotypic characters based on similarity coefficient were divided into four clusters with 70 per cent similarity. By means of similarity coefficients (SG), at 70 per cent phenon level the genotypes were broadly grouped into four clusters i.e., cluster I and cluster II both comprising of a single variety Bhaskara and Chintamani-1 respectively, cluster III having six varieties and cluster IV with 17 varieties. The analysis using ISSR markers allowed us to distinguish 25 varieties. A total of 81 distinct DNA fragments ranging from 0.1 to 2.5 kb were amplified by using 10 selected ISSR primers. Genetic similarity analysis was conducted for the presence or absence of bands in the ISSR profile. Cluster analysis clearly showed that 25 varieties of cashew could be grouped into two major clusters based on similarity indices. The first major cluster consists of Priyanka and Madakkathara-1, two moderate yielding varieties. The other major cluster was divided into two sub-minor clusters, one sub-minor cluster having single variety and the other sub-minor cluster was represented by 22 varieties. Among the 25 varieties, Kanaka and Vridhachalam-3 showed the highest similarity indices (92%). The analysis of genetic relationships in cashew using morphological traits and ISSR banding data can be useful for plant improvement, descriptions of new varieties and also for assessment of varietal purity in plant certification programmes

Analysis of genetic relationships in cashew varieties using morphological characters and ISSR markers

In the present paper genetic relationships of twenty five varieties of cashew are described on the basis of morphological characters and ISSR markers. Results obtained for the phenotypic characters based on similarity coefficient were divided into four clusters with 70 per cent similarity. By means of similarity coefficients (SG), at 70 per cent phenon level the genotypes were broadly grouped into four clusters i.e., cluster I and cluster II both comprising of a single variety Bhaskara and Chintamani-1 respectively, cluster III having six varieties and cluster IV with 17 varieties. The analysis using ISSR markers allowed us to distinguish 25 varieties. A total of 81 distinct DNA fragments ranging from 0.1 to 2.5 kb were amplified by using 10 selected ISSR primers. Genetic similarity analysis was conducted for the presence or absence of bands in the ISSR profile. Cluster analysis clearly showed that 25 varieties of cashew could be grouped into two major clusters based on similarity indices. The first major cluster consists of Priyanka and Madakkathara-1, two moderate yielding varieties. The other major cluster was divided into two sub-minor clusters, one sub-minor cluster having single variety and the other sub-minor cluster was represented by 22 varieties. Among the 25 varieties, Kanaka and Vridhachalam-3 showed the highest similarity indices (92%). The analysis of genetic relationships in cashew using morphological traits and ISSR banding data can be useful for plant improvement, descriptions of new varieties and also for assessment of varietal purity in plant certification programmes

EVALUATION OF PLANT EXTRACTS AGAINST LUNG CANCER USING H460 CELL LINE

Mammalian tumor cells show imperviousness to chemotherapy and its serious symptoms lessens the clinical adequacy of an extensive assortment of anticancer drugs. Plant-derived compounds show numerous valuable impacts and can restrain a few phases of malignancy of cancer. Despite there is paramount progress in cancer therapeutics in the last decades, the need to find, grow new and synergistic plant predicated anticancer specialists are in the developing stage. In the present study, we endeavored to exploit bioactive compounds of two plants viz. Limonia acidissima and Syzygium cumini, investigated their antiproliferative properties for lung cancer and evaluated the haemolytic activity. Methanol extracts of these plants were experimented for cytotoxic activity and found that L.acidissima and S.cumini selectively inhibited H 460 cell proliferation and found that the two plants are non-haemolytic. Our study deduce that the potential bioactive compounds of these plants have vibrant chance to fight lung cancer as were seen to be non-haemolytic to blood cells and they are very much recorded as a traditional medicine for various therapeutic uses

Public perception of bioenergy in North Carolina and Tennessee

© 2016, The Author(s). Background: The goal of the study is to examine the general public’s understanding and perceptions of bioenergy and biofuels in North Carolina (NC) and Tennessee (TN). The study focuses on the public concerns, support and risk evaluations of alternative bioenergy feedstocks and biofuels, and includes an assessment of the economic, environmental, social, and policy impacts of bioenergy production and use. Methods: A sample of consumers in NC and TN were surveyed in the fall of 2013 and spring of 2014 for their perceptions about bioenergy and specifically, biofuels for transportation. Five hundred eighty-six consumers completed the questionnaire electronically (376 in NC and 210 in TN). Results: Respondents reported that the price and vehicle compatibility with biomass-based transportation fuels were the most important factors in their choice of biofuels over gasoline at a pump. Results show that the acceptance of bioenergy depends on the extent of knowledge and available information to consumers about the energy source. A principal component analysis (PCA) indicated seven distinct dimensions of consumer’s perception about bioenergy. The key dimensions are the following: how bioenergy benefits the society, risks of bioenergy use, government support for bioenergy, increase in food cost, conditional use of trees, support for low-cost biofuel alternative to current energy, and market attributes of bioenergy purchase. Conclusions: The findings from this study reflect the need for communicating the benefits and risks from the use of bioenergy to the general public through trustworthy channels of communication and targeted policy, market, and institutional support