Deamidation of Proteins: The crystal structure of bovine pancreatic ribonuclease with an isoaspartyl residue at position 67

The non-enzymatic deamidation of asparagine residues in proteins is a widely occurring reaction, both in vivo and in vitro. Although the importance of this process is commonly recognised, only little structural information is available on it. In order to evaluate the structural effects of this reaction in proteins, we have determined the crystal structure of a ribonuclease A derivative in which asparagine 67 has been replaced by an isoaspartyl residue, as a consequence of an in vitro deamidation reaction. The overall structure of the model, refined to a crystallographic R-factor of 0.159 at a resolution of 1.9 Å, is very similar to that of the native protein, but considerable deviations are observed in the region delimited by the disulphide bridge 65–72. In particular, the insertion of an extra methylene group in the main chain at residue 67 breaks up the hydrogen bond network that makes this region rather rigid in ribonuclease A. On the basis of the structure observed, some of the slightly but significantly different properties of this deamidated derivative, with respect to the native enzyme, can be explained

Measurement of the pseudoscalar mixing angle and η′\eta^{\prime} gluonium content with KLOE detector

We have measured the ratio $R_{\phi}=BR(\phi \to \eta^{\prime} \gamma)/BR(\phi \to \eta \gamma)$ by looking for the radiative decays $\phi \to \eta^{\prime} \gamma$ and $\phi \to \eta \gamma$ in the final states $\pi^+\pi^-$ 7 $\gamma$'s and 7 $\gamma$'s respectively, in a sample of $\sim 1.3\cdot 10^{9}$ $\phi$ mesons produced at the Frascati $\phi$-factory. We obtain $R_{\phi}=(4.77\pm0.09_{stat}\pm0.19_{sys})\cdot 10^{-3}$ from which we derive $BR(\phi \to \eta^{\prime} \gamma)=(6.20\pm0.11_{stat}\pm0.25_{sys})\cdot 10^{-5}$. In the hypothesis of no gluonium content we extract the pseudoscalar mixing angle in the quark-flavor basis $\phi_P=(41.4\pm0.3_{stat}\pm0.7_{sys}\pm0.6_{th})^{\circ}$. Combining the value of $R_{\phi}$ with other constraints, we estimate the gluonium fractional content of $\eta^{\prime}$ meson as $Z^2 = 0.14\pm0.04$ and the mixing angle $\phi_P = (39.7\pm0.7)^{\circ}$.Comment: 13 pages, 8 figure

A global fit to determine the pseudoscalar mixing angle and the gluonium content of the eta' meson

We update the values of the eta-eta' mixing angle and of the eta' gluonium content by fitting our measurement R_phi = BR(phi to eta' gamma)/ BR(phi to eta gamma) together with several vector meson radiative decays to pseudoscalars (V to P gamma), pseudoscalar mesons radiative decays to vectors (P to V gamma) and the eta' to gamma gamma, pi^0 to gamma gamma widths. From the fit we extract a gluonium fraction of Z^2_G = 0.12 +- 0.04, the pseudoscalar mixing angle psi_P = (40.4 +- 0.6) degree and the phi-omega mixing angle psi_V = (3.32 +- 0.09) degree. Z^2_G and psi_P are fairly consistent with those previously published. We also evaluate the impact on the eta' gluonium content determination of future experimental improvements of the eta' branching ratios and decay width.Comment: 13 pages, 7 figures to submit to JHE

Charged kaon lifetime at KLOE

Preliminary result on the charged kaon lifetime, obtained by the KLOE experiment operating at DA$\Phi$NE, the Frascati $\phi$-factory, is presentedComment: 3 pages, 3 figures, to appear in the proceedings of 42nd Rencontres de Moriond on Electroweak Interactions and Unified Theories, La Thuile, Aosta Valley, Italy, 10-17 Mar 200

Determination of CP and CPT violation parameters in the neutral kaon system using the Bell-Steinberger relation and data from the KLOE experiment

We present an improved determination of the CP and CPT violation parameters Re(epsilon) and Im(delta) based on the unitarity condition (Bell-Steinberger relation) and on recent results from the KLOE experiment. We find Re(epsilon) = (159.6 \pm 1.3)10^-5 and Im(delta) = (0.4 \pm 2.1)10^-5, consistent with no CPT violation.Comment: Submitted to JHE

Measurements of the Absolute Branching Ratios for the Dominant KL Decays, the KL Lifetime, and Vus with the KLOE Detector

From a sample of about 10^9 Phi mesons produced at DAFNE, we have selected KL mesons tagged by observing KS->pi+pi- decays. We present results on the major KL branching ratios, including those of the semileptonic decays needed for the determination of Vus. These branching ratio measurements are fully inclusive with respect to final-state radiation. The KL lifetime has also been measured.Comment: Submitted to Phys. Lett.