Protein connectivity in chemotaxis receptor complexes

The chemotaxis sensory system allows bacteria such as Escherichia coli to swim towards nutrients and away from repellents. The underlying pathway is remarkably sensitive in detecting chemical gradients over a wide range of ambient concentrations. Interactions among receptors, which are predominantly clustered at the cell poles, are crucial to this sensitivity. Although it has been suggested that the kinase CheA and the adapter protein CheW are integral for receptor connectivity, the exact coupling mechanism remains unclear. Here, we present a statistical-mechanics approach to model the receptor linkage mechanism itself, building on nanodisc and electron cryotomography experiments. Specifically, we investigate how the sensing behavior of mixed receptor clusters is affected by variations in the expression levels of CheA and CheW at a constant receptor density in the membrane. Our model compares favorably with dose-response curves from in vivo Förster resonance energy transfer (FRET) measurements, demonstrating that the receptor-methylation level has only minor effects on receptor cooperativity. Importantly, our model provides an explanation for the non-intuitive conclusion that the receptor cooperativity decreases with increasing levels of CheA, a core signaling protein associated with the receptors, whereas the receptor cooperativity increases with increasing levels of CheW, a key adapter protein. Finally, we propose an evolutionary advantage as explanation for the recently suggested CheW-only linker structures

Spin-Orbit Coupling and the Evolution of Transverse Spin

We investigate the evolution of transverse spin in tightly focused circularly polarized beams of light, where spin-orbit coupling causes a local rotation of the polarization ellipses upon propagation through the focal volume. The effect can be explained as a relative Gouy-phase shift between the circularly polarized transverse field and the longitudinal field carrying orbital angular momentum. The corresponding rotation of the electric transverse spin density is observed experimentally by utilizing a recently developed reconstruction scheme, which relies on transverse-spin-dependent directional scattering of a nano-probe.Comment: 4 pages, 2 figure

A noise-immune cavity-assisted non-destructive detection for an optical lattice clock in the quantum regime

We present and implement a non-destructive detection scheme for the transition probability readout of an optical lattice clock. The scheme relies on a differential heterodyne measurement of the dispersive properties of lattice-trapped atoms enhanced by a high finesse cavity. By design, this scheme offers a 1st order rejection of the technical noise sources, an enhanced signal-to-noise ratio, and an homogeneous atom-cavity coupling. We theoretically show that this scheme is optimal with respect to the photon shot noise limit. We experimentally realize this detection scheme in an operational strontium optical lattice clock. The resolution is on the order of a few atoms with a photon scattering rate low enough to keep the atoms trapped after detection. This scheme opens the door to various different interrogations protocols, which reduce the frequency instability, including atom recycling, zero-dead time clocks with a fast repetition rate, and sub quantum projection noise frequency stability

Universal Loss Dynamics in a Unitary Bose Gas

The low temperature unitary Bose gas is a fundamental paradigm in few-body and many-body physics, attracting wide theoretical and experimental interest. Here we first present a theoretical model that describes the dynamic competition between two-body evaporation and three-body re-combination in a harmonically trapped unitary atomic gas above the condensation temperature. We identify a universal magic trap depth where, within some parameter range, evaporative cooling is balanced by recombination heating and the gas temperature stays constant. Our model is developed for the usual three-dimensional evaporation regime as well as the 2D evaporation case. Experiments performed with unitary 133 Cs and 7 Li atoms fully support our predictions and enable quantitative measurements of the 3-body recombination rate in the low temperature domain. In particular, we measure for the first time the Efimov inelasticity parameter $\eta$ * = 0.098(7) for the 47.8-G d-wave Feshbach resonance in 133 Cs. Combined 133 Cs and 7 Li experimental data allow investigations of loss dynamics over two orders of magnitude in temperature and four orders of magnitude in three-body loss. We confirm the 1/T 2 temperature universality law up to the constant $\eta$ *

Lifetime of the Bose Gas with Resonant Interactions

We study the lifetime of a Bose gas at and around unitarity using a Feshbach resonance in lithium~7. At unitarity, we measure the temperature dependence of the three-body decay coefficient $L_{3}$. Our data follow a $L_3 {=} \lambda_{3} / T^{2}$ law with \lambda_{3} = 2.5(3)_{stat}_(6)_{sys} 10^{-20} (\mu K)^2 cm^6 s^{-1} and are in good agreement with our analytical result based on the zero-range theory. Varying the scattering length $a$ at fixed temperature, we investigate the crossover between the finite-temperature unitary region and the previously studied regime where $|a|$ is smaller than the thermal wavelength. We find that $L_{3}$ is continuous across resonance, and over the whole $a {<} 0$ range our data quantitatively agree with our calculation

Large atom number dual-species magneto-optical trap for fermionic 6Li and 40K atoms

We present the design, implementation and characterization of a dual-species magneto-optical trap (MOT) for fermionic 6Li and 40K atoms with large atom numbers. The MOT simultaneously contains 5.2x10^9 6Li-atoms and 8.0x10^9 40K-atoms, which are continuously loaded by a Zeeman slower for 6Li and a 2D-MOT for 40K. The atom sources induce capture rates of 1.2x10^9 6Li-atoms/s and 1.4x10^9 40K-atoms/s. Trap losses due to light-induced interspecies collisions of ~65% were observed and could be minimized to ~10% by using low magnetic field gradients and low light powers in the repumping light of both atomic species. The described system represents the starting point for the production of a large-atom number quantum degenerate Fermi-Fermi mixture

Constructing a chiral dipolar mode in an achiral nanostructure

We discuss the excitation of a chiral dipolar mode in an achiral silicon nanoparticle. In particular, we make use of the electric and magnetic polarizabilities of the silicon nanoparticle to construct this chiral electromagnetic mode which is conceptually similar to the fundamental modes of 3D chiral nanostructures or molecules. We describe the chosen tailored excitation with a beam carrying neither spin nor orbital angular momentum and investigate the emission characteristics of the chiral dipolar mode in the helicity basis, consisting of parallel electric and magnetic dipole moments, phase shifted by $\pm \pi/2$. We demonstrate the wavelength dependence and measure the spin and orbital angular momentum in the emission of the excited chiral mode.Comment: 6 pages, 3 figure

Orbital-to-Spin Angular Momentum Conversion Employing Local Helicity

Spin-orbit interactions in optics traditionally describe an influence of the polarization degree of freedom of light on its spatial properties. The most prominent example is the generation of a spin-dependent optical vortex upon focusing or scattering of a circularly polarized plane-wave by a nanoparticle, converting spin to orbital angular momentum of light. Here, we present a mechanism of conversion of orbital-to-spin angular momentum of light upon scattering of a linearly polarized vortex beam by a spherical silicon nanoparticle. We show that focused linearly polarized Laguerre-Gaussian beams of first order ($\ell = \pm 1$) exhibit an $\ell$-dependent spatial distribution of helicity density in the focal volume. By using a dipolar scatterer the helicity density can be manipulated locally, while influencing globally the spin and orbital angular momentum of the beam. Specifically, the scattered light can be purely circularly polarized with the handedness depending on the orbital angular momentum of the incident beam. We corroborate our findings with theoretical calculations and an experimental demonstration. Our work sheds new light on the global and local properties of helicity conservation laws in electromagnetism.Comment: 8 pages, 6 figures, 1 tabl

An all-solid-state laser source at 671 nm for cold atom experiments with lithium

We present an all solid-state narrow line-width laser source emitting $670\,\mathrm{mW}$ output power at $671\,\mathrm{nm}$ delivered in a diffraction-limited beam. The \linebreak source is based on a fre-quency-doubled diode-end-linebreak pumped ring laser operating on the ${^4F}_{3/2} \rightarrow {^4I}_{13/2}$ transition in Nd:YVO$_4$. By using periodically-poled po-tassium titanyl phosphate (ppKTP) in an external build-up cavity, doubling efficiencies of up to 86% are obtained. Tunability of the source over $100\,\rm GHz$ is accomplished. We demonstrate the suitability of this robust frequency-stabilized light source for laser cooling of lithium atoms. Finally a simplified design based on intra-cavity doubling is described and first results are presented