Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

Water quality assessment requires a continuous and strict analysis of samples to guarantee compliance with established standards. Nowadays, the increasing number of pollutants and their synergistic effects lead to the development general toxicity bioassays capable to analyse water pollution as a whole. Current general toxicity methods, e.g. Microtox®, rely on long operation protocols, the use of complex and expensive instrumentation and sample pre-treatment, which should be transported to the laboratory for analysis. These requirements delay sample analysis and hence, the response to avoid an environmental catastrophe. In an attempt to solve it, a fast (15 min) and low-cost toxicity bioassay based on the chromatic changes associated to bacterial ferricyanide reduction is here presented. E. coli cells (used as model bacteria) were stably trapped on low-cost paper matrices (cellulose-based paper discs, PDs) and remained viable for long times (1 month at -20 °C). Apart from bacterial carrier, paper matrices also acted as a fluidic element, allowing fluid management without the need of external pumps. Bioassay evaluation was performed using copper as model toxic agent. Chromatic changes associated to bacterial ferricyanide reduction were determined by three different transduction methods, i.e. (i) optical reflectometry (as reference method), (ii) image analysis and (iii) visual inspection. In all cases, bioassay results (in terms of half maximal effective concentrations, EC50) were in agreement with already reported data, confirming the good performance of the bioassay. The validation of the bioassay was performed by analysis of real samples from natural sources, which were analysed and compared with a reference method (i.e. Microtox). Obtained results showed agreement for about 70% of toxic samples and 80% of non-toxic samples, which may validate the use of this simple and quick protocol in the determination of general toxicity. The minimum instrumentation requirements and the simplicity of the bioassay open the possibility of in-situ water toxicity assessment with a fast and low-cost protocolPostprint (author's final draft

Coupled dark matter-dark energy in light of near Universe observations

Cosmological analysis based on currently available observations are unable to rule out a sizeable coupling among the dark energy and dark matter fluids. We explore a variety of coupled dark matter-dark energy models, which satisfy cosmic microwave background constraints, in light of low redshift and near universe observations. We illustrate the phenomenology of different classes of dark coupling models, paying particular attention in distinguishing between effects that appear only on the expansion history and those that appear in the growth of structure. We find that while a broad class of dark coupling models are effectively models where general relativity (GR) is modified --and thus can be probed by a combination of tests for the expansion history and the growth of structure--, there is a class of dark coupling models where gravity is still GR, but the growth of perturbations is, in principle modified. While this effect is small in the specific models we have considered, one should bear in mind that an inconsistency between reconstructed expansion history and growth may not uniquely indicate deviations from GR. Our low redshift constraints arise from cosmic velocities, redshift space distortions and dark matter abundance in galaxy voids. We find that current data constrain the dimensionless coupling to be |xi|<0.2, but prospects from forthcoming data are for a significant improvement. Future, precise measurements of the Hubble constant, combined with high-precision constraints on the growth of structure, could provide the key to rule out dark coupling models which survive other tests. We shall exploit as well weak equivalence principle violation arguments, which have the potential to highly disfavour a broad family of coupled models.Comment: 34 pages, 6 figures; changes to match published versio

The GAPS Programme with HARPS-N@TNG IX. The multi-planet system KELT-6: detection of the planet KELT-6 c and measurement of the Rossiter-McLaughlin effect for KELT-6 b

Aims. For more than 1.5 years we monitored spectroscopically the star KELT-6 (BD+312447), known to host the transiting hot Saturn KELT-6b, because a previously observed long-term trend in radial velocity time series suggested the existence of an outer companion. Methods. We collected a total of 93 new spectra with the HARPS-N and TRES spectrographs. A spectroscopic transit of KELT-6b was observed with HARPS-N, and simultaneous photometry was obtained with the IAC-80 telescope. Results. We proved the existence of an outer planet with a mininum mass M$_{\rm p}$sini=3.71$\pm$0.21 M$_{\rm Jup}$ and a moderately eccentric orbit ($e=0.21_{-0.036}^{+0.039}$) of period P$\sim$3.5 years. We improved the orbital solution of KELT-6b and obtained the first measurement of the Rossiter-McLaughlin effect, showing that the planet has a likely circular, prograde, and slightly misaligned orbit, with a projected spin-orbit angle $\lambda$=$-$36$\pm$11 degrees. We improved the KELT-6b transit ephemeris from photometry, and we provided new measurements of the stellar parameters. KELT-6 appears as an interesting case to study the formation and evolution of multi-planet systems.Comment: Letter, 4 figures, accepted for publication in A&A. Some language editing and numbering of the paper series changed (from X to IX

The role of the land-surface model for climate change projections over the Iberian Peninsula

The importance of land-surface processes within Regional Climate Models for accurately reproducing the present-day climate is well known. However, their role when projecting future climate is still poorly reported. Hence, this work assesses the influence of the land-surface processes, particularly the contribution of soil moisture, when projecting future changes for temperature, precipitation and wind over a complex area as the Iberian Peninsula, which, in addition, shows great sensitivity to climate change. The main signals are found for the summer season, when the results indicate a strengthening in the increases projected for both mean temperature and temperature variability as a consequence of the future intensification of the positive soil moisture-temperature feedback. The more severe warming over the inner dry Iberian Peninsula further implies an intensification of the Iberian thermal low and, thus, of the cyclonic circulation. Furthermore, the land-atmosphere coupling leads to the projection of a wider future daily temperature range, since maximum temperatures are more affected than minima, a feature absent in non-coupled simulations. Regarding variability, the areas where the land-atmosphere coupling introduces larger changes are those where the reduction in the soil moisture content is more dramatic in future simulations, i.e., the so-called transitional zones. As regards precipitation, weaker positive signals for convective precipitation and more intense negative signals for non-convective precipitation are obtained as a result of the soil moisture-atmosphere interactions. These results highlight the crucial contribution of soil moisture to climate change projections and suggest its plausible key role for future projections of extreme events

Understanding the Role of AMPA Receptors in Autism: Insights from Circuit and Synapse Dysfunction

Autism spectrum disorders represent a diverse etiological spectrum that converge on a syndrome characterized by discrepant deficits in developmental domains often highlighted by concerns in socialization, sensory integration, and autonomic functioning. Importantly, the incidence and prevalence of autism spectrum disorders have seen sharp increases since the syndrome was first described in the 1940s. The wide etiological spectrum and rising number of individuals being diagnosed with the condition lend urgency to capturing a more nuanced understanding of the pathogenic mechanisms underlying the autism spectrum disorders. The current review seeks to understand how the disruption of AMPA receptor (AMPAr)-mediated neurotransmission in the cerebro-cerebellar circuit, particularly in genetic autism related to SHANK3 or SYNGAP1 protein dysfunction function and autism associated with in utero exposure to the anti-seizure medications valproic acid and topiramate, may contribute to the disease presentation. Initially, a discussion contextualizing AMPAr signaling in the cerebro-cerebellar circuitry and microstructural circuit considerations is offered. Subsequently, a detailed review of the literature implicating mutations or deletions of SHANK3 and SYNGAP1 in disrupted AMPAr signaling reveals how bidirectional pathogenic modulation of this key circuit may contribute to autism. Finally, how pharmacological exposure may interact with this pathway, via increased risk of autism diagnosis with valproic acid and topiramate exposure and potential treatment of autism using AMPAr modulator perampanel, is discussed. Through the lens of the review, we will offer speculation on how neuromodulation may be used as a rational adjunct to therapy. Together, the present review seeks to synthesize the disparate considerations of circuit understanding, genetic etiology, and pharmacological modulation to understand the mechanistic interaction of this important and complex disorder

Secular evolution versus hierarchical merging: galaxy evolution along the Hubble sequence, in the field and rich environments

In the current galaxy formation scenarios, two physical phenomena are invoked to build disk galaxies: hierarchical mergers and more quiescent external gas accretion, coming from intergalactic filaments. Although both are thought to play a role, their relative importance is not known precisely. Here we consider the constraints on these scenarios brought by the observation-deduced star formation history on the one hand, and observed dynamics of galaxies on the other hand: the high frequency of bars and spirals, the high frequency of perturbations such as lopsidedness, warps, or polar rings. All these observations are not easily reproduced in simulations without important gas accretion. N-body simulations taking into account the mass exchange between stars and gas through star formation and feedback, can reproduce the data, only if galaxies double their mass in about 10 Gyr through gas accretion. Warped and polar ring systems are good tracers of this accretion, which occurs from cold gas which has not been virialised in the system's potential. The relative importance of these phenomena are compared between the field and rich clusters. The respective role of mergers and gas accretion vary considerably with environment.Comment: 18 pages, 8 figures, review paper to "Penetrating Bars through Masks of Cosmic Dust: the Hubble Tuning Fork Strikes a New Note", Pilanesberg, ed. D. Block et al., Kluwe

The Atacama Cosmology Telescope (ACT): Beam Profiles and First SZ Cluster Maps

The Atacama Cosmology Telescope (ACT) is currently observing the cosmic microwave background with arcminute resolution at 148 GHz, 218 GHz, and 277 GHz. In this paper, we present ACT's first results. Data have been analyzed using a maximum-likelihood map-making method which uses B-splines to model and remove the atmospheric signal. It has been used to make high-precision beam maps from which we determine the experiment's window functions. This beam information directly impacts all subsequent analyses of the data. We also used the method to map a sample of galaxy clusters via the Sunyaev-Zel'dovich (SZ) effect, and show five clusters previously detected with X-ray or SZ observations. We provide integrated Compton-y measurements for each cluster. Of particular interest is our detection of the z = 0.44 component of A3128 and our current non-detection of the low-redshift part, providing strong evidence that the further cluster is more massive as suggested by X-ray measurements. This is a compelling example of the redshift-independent mass selection of the SZ effect.Comment: 16 pages, 10 figures. Accepted for publication in ApJS. See Marriage et al. (arXiv:1010.1065) and Menanteau et al. (arXiv:1006.5126) for additional cluster result

Impact of elite soccer coaching change on team performance according to coach- and club-related variables

A coaching change is an extreme, but frequently occurring phenomenon in elite soccer with its impact on team success debatable. The aim of the current study was twofold: (i) to compare team's performance when coached by new and old coaches; and (ii) to investigate the impact of a coaching change on team's performance according to coach- and club-related factors. All in-season coaching changes from the 2010-11 to 2017-18 seasons within the Spanish, French, English, German and Italian professional leagues were examined. Team performance was assessed as points awarded from match outcome over 1-20 matches prior to and following the coaching change. Four independent variables (coach's experience, team's budget, whether the coach had been an elite former player or not, and whether the coach was a novice or not) were included into linear regression modelling. The main results showed that team's short-term performance was improved significantly with a change to a new coach with this impact declining in the longer term (> 10 matches). Specifically, the number of points (1.15-1.32 vs. 0.37-1.03, p < 0.05) and the moving average of points (1.19-1.31 vs. 0.37-1.04, p < 0.05) awarded per match were significantly greater after the coaching change. Further, the winning effect due to the new coach was independent of coach-related factors such as coaching experience or the new coach being a former elite player. A critical organisational decision to change coaches may provide an essential stimulus for future team success in elite soccer

Lectures on Cosmic Inflation and its Potential Stringy Realizations

These notes present a brief introduction to Hot Big Bang cosmology and Cosmic Inflation, together with a selection of some recent attempts to embed inflation into string theory. They provide a partial description of lectures presented in courses at Dubrovnik in August 2006, at CERN in January 2007 and at Cargese in August 2007. They are aimed at graduate students with a working knowledge of quantum field theory, but who are unfamiliar with the details of cosmology or of string theory.Comment: 68 pages, lectures given at Dubrovnik, Aug 2006; CERN, January 2007; and Cargese, Aug 200

Searching for a Cosmological Preferred Axis: Union2 Data Analysis and Comparison with Other Probes

We review, compare and extend recent studies searching for evidence for a preferred cosmological axis. We start from the Union2 SnIa dataset and use the hemisphere comparison method to search for a preferred axis in the data. We find that the hemisphere of maximum accelerating expansion rate is in the direction $(l,b)=({309^\circ}^{+23^\circ}_{-3^\circ}, {18^\circ}^{+11^\circ}_{-10^\circ})$ (\omm=0.19) while the hemisphere of minimum acceleration is in the opposite direction $(l,b)=({129^\circ}^{+23^\circ}_{-3^\circ},{-18^\circ}^{+10^\circ}_{-11^\circ})$ (\omm=0.30). The level of anisotropy is described by the normalized difference of the best fit values of \omm between the two hemispheres in the context of \lcdm fits. We find a maximum anisotropy level in the Union2 data of \frac{\Delta \ommax}{\bomm}=0.43\pm 0.06. Such a level does not necessarily correspond to statistically significant anisotropy because it is reproduced by about $30$ of simulated isotropic data mimicking the best fit Union2 dataset. However, when combined with the axes directions of other cosmological observations (bulk velocity flow axis, three axes of CMB low multipole moments and quasar optical polarization alignment axis), the statistical evidence for a cosmological anisotropy increases dramatically. We estimate the probability that the above independent six axes directions would be so close in the sky to be less than $1$. Thus either the relative coincidence of these six axes is a very large statistical fluctuation or there is an underlying physical or systematic reason that leads to their correlation.Comment: 10 pages, 7 figures. Accepted in JCAP (to appear). Extended analysis with redshift tomography of SnIa, included errorbars and increased number of axes. The Mathematica 7 files with the data used for the production of the figures along with a Powerpoint file with additional figures may be downloaded from http://leandros.physics.uoi.gr/anisotrop