Identification of unprecedented anticancer properties of high molecular weight biomacromolecular complex containing bovine lactoferrin (HMW-bLf)

With the successful clinical trials, multifunctional glycoprotein bovine lactoferrin is gaining attention as a safe nutraceutical and biologic drug targeting cancer, chronic-inflammatory, viral and microbial diseases. Interestingly, recent findings that human lactoferrin oligomerizes under simulated physiological conditions signify the possible role of oligomerization in the multifunctional activities of lactoferrin molecule during infections and in disease targeting signaling pathways. Here we report the purification and physicochemical characterization of high molecular weight biomacromolecular complex containing bovine lactoferrin (≥250 kDa), from bovine colostrum, a naturally enriched source of lactoferrin. It showed structural similarities to native monomeric iron free (Apo) lactoferrin (∼78-80 kDa), retained anti-bovine lactoferrin antibody specific binding and displayed potential receptor binding properties when tested for cellular internalization. It further displayed higher thermal stability and better resistance to gut enzyme digestion than native bLf monomer. High molecular weight bovine lactoferrin was functionally bioactive and inhibited significantly the cell proliferation (p<0.01) of human breast and colon carcinoma derived cells. It induced significantly higher cancer cell death (apoptosis) and cytotoxicity in a dose-dependent manner in cancer cells than the normal intestinal cells. Upon cellular internalization, it led to the up-regulation of caspase-3 expression and degradation of actin. In order to identify the cutting edge future potential of this bio-macromolecule in medicine over the monomer, its in-depth structural and functional properties need to be investigated further

Effect of Varied Phases of Lunar on the Growth and Yield Parameters of Rice Varieties

Aim: To understand and reason out the effect of lunar phases on the rice crop growth and development. Study Design: Experiment was laid out in Factorial Randomized Block Design. Place and Duration of Study: Field trial was conducted in the wetland farm of Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu during the Navarai season (Dec. 2021 – Apr. 2022). Methodology: Treatment consists of two factors viz., Sowing date (4 Nos.) weekly sowing with respect to lunar phases – New moon, first quarter, full moon and third quarter) and Varieties (4 Nos.) in which two organic (i.e. Kullakar, Karunkuruvai) and two conventional varieties (ADT 43, ASD 16). Normal cultivation practices were followed as per the Tamil Nadu Crop Production Guide 2021. Results: The pooled mean value revealed that the seeds sown at full moon gave significant positive influence on the growth parameters such as plant height, number of tillers, leaf area index and dry matter production compared to other sown dates. Whereas the treatments that had flowering stage synchronized with full moon phase were produced more yield and dry matter content. Conclusion: The rice varieties sown in full moon phase exhibited superior growth on compared to other lunar phases and the treatments had flowering synced with full moon phase resulted with more yield and dry matter production

Aptamer-based therapeutics of the past, present and future: from the perspective of eye-related diseases

 Aptamers have emerged as a novel and powerful class of biomolecules with an immense untapped potential. The ability to synthesise highly specific aptamers against any molecular target make them a vital cog in the design of effective therapeutics for the future. However, only a minutia of the enormous potential of this dynamic class of molecule has been exploited. Several aptamers have been studied for the treatment of eye-related disorders, and one such strategy has been successful in therapy. This review gives an account of several eye diseases and their regulatory biomolecules where other nucleic acid therapeutics have been attempted with limited success and how aptamers, with their exceptional flexibility to chemical modifications, can overcome those inherent shortcomings