Callus induction and in vitro mass culture of adventitious roots from leaf segment explants of Dendropanax morbifera Lev.

Dendropanax morbifera Lev. is a unique species and natively found in Korea and distributed in the South regions, such as Jeju, Goheung, and Wando. In this study, tissue culture system for native D. morbifera was developed. The callus from native D. morbifera leaves was cultured on Woody Plant Media (WPM) supplemented with 30 g L–1 of sucrose, with addition of 2.4-D and BA [(0.5, 1.0, 2.0) mg L–1 ], separately and mixed. After 5 wk of culture, the highest induction of callus was obtained from 0.5 mg L–1 of 2.4-D mixed with 2.0 mg L–1 of BA. Adventitious root formation on different media (MS, WPM, B5) with various auxins (IBA, IAA, and NAA) and different concentration [(0, 1, 3, 5) mg L–1 ] were tested. After 8 wk of culture, WPM showed better induction of adventitious root. The highest induction of adventitious root was obtained on 3.0 mg L–1 IBA. Root growth was best in WPM liquid medium with 3.0 mg L–1 of IBA and 30 g L–1 of sucrose. The same formulation with modified ½ WPM was successfully established in vitro adventitious roots culture in 18-L bioreactor system. This study also proposed as the mass production technique of adventitious roots from native D. morbifera

Design, synthesis of Dioxopiperidinamide derivatives by amide coupling reaction and study of their biological activity

Abstract A series of fifteen dioxopiperidinamide derivatives synthesized and their spectra chemical data were reported. An alternative amide coupling reaction has been followed by using 3-aminopiperidine-2,6-dione through amide bond functionalization between N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC.HCl) and Hydroxybenzotriazole (HOBt). All the designed compounds were docked in to the active sites of 2A73: Human Complement Component C3. All the docked compounds is favorable to make both hydrogen and hydrophobic interaction with amino acids located at the binding site. The most of potential compounds 4g, 4h and 7 studied for their anticancer activity on the THP-1 cancer cells line. It was also further established their Anti-inflammatory activity and Antioxidant activity with the comparable efficiency against those of clinically used drugs.</jats:p