Histochemical data on the skin mucous cells during the development of the trout [Translation from: Bollettino della Societa Italiana di Biologia Sperimentale 45, 1273-1276, 1969]

In the skin of Salmo irideus the production of mucus is due to one type of cell specialized as a mucus cell. The histochemical research presented in this paper describes the mucous cells of Salmo irideus and demonstrates observable variation in such cells during the fish's development

Ileocecal appendix involvement in fabry disease mimicking an acute abdomen

Anderson-Fabry disease (AFD) is a rare, X-linked, lysosomal storage disorder due to a deficiency of alphagalactosidase A. The direct consequence is a lipid storage with the accumulation of glycosphingolipids throughout the body. The clinical picture is highly variable and depends on cellular storage deposition ranging from neurological, cutaneous and renal symptoms to cardiac and gastrointestinal ones. We are reporting about the case of a young female carrier of alpha-galactosidase A (agalA) gene mutation who was treated at our out-clinic practice for minimal neurological involvement (achroparaestesia). She was subsequently admitted in order to undergo appendectomy because of an acute severe abdominal pain. The histological examination of her appendix revealed only a deposition of globotriaosylceramide (Gb3) without any sign of acute inflammation. This case confirms the extreme clinical variability of Fabry disease and how the gastrointestinal involvement diagnosis can be misse

First evidence of fully spatially mixed first and second generations in globular clusters: the case of NGC 6362

We present the first evidence of multiple populations in the Galactic globular cluster NGC 6362. We used optical and near-UV Hubble Space Telescope and ground based photometry, finding that both the sub giant and red giant branches are split in two parallel sequences in all color magnitude diagrams where the F336W filter (or U band) is used. This cluster is one of the least massive globulars (M_tot~5x10^4 M_sun) where multiple populations have been detected so far. Even more interestingly and at odds with any previous finding, we observe that the two identified populations share the same radial distribution all over the cluster extension. NGC 6362 is the first system where stars from different populations are found to be completely spatially mixed. Based on N-body and hydrodynamical simulations of multiple stellar generations, we argue that, to reproduce these findings, NGC 6362 should have lost up to the 80% of its original massComment: Accepted for publication by ApJ Letters; 6 pages, 5 figure

Double Blue Straggler sequences in GCs: the case of NGC 362

We used high-quality images acquired with the WFC3 on board the HST to probe the blue straggler star (BSS) population of the Galactic globular cluster NGC 362. We have found two distinct sequences of BSS: this is the second case, after M 30, where such a feature has been observed. Indeed the BSS location, their extension in magnitude and color and their radial distribution within the cluster nicely resemble those observed in M 30, thus suggesting that the same interpretative scenario can be applied: the red BSS sub-population is generated by mass transfer binaries, the blue one by collisions. The discovery of four new W UMa stars, three of which lying along the red-BSS sequence, further supports this scenario. We also found that the inner portion of the density profile deviates from a King model and is well reproduced by either a mild power-law (\alpha -0.2) or a double King profile. This feature supports the hypothesis that the cluster is currently undergoing the core collapse phase. Moreover, the BSS radial distribution shows a central peak and monotonically decreases outward without any evidence of an external rising branch. This evidence is a further indication of the advanced dynamical age of NGC 362: in fact, together with M 30, NGC 362 belongs to the family of dynamically old clusters (Family III) in the "dynamical clock" classification proposed by Ferraro et al. (2012). The observational evidence presented here strengthens the possible connection between the existence of a double BSS sequence and a quite advanced dynamical status of the parent cluster.Comment: Accepted for publication by ApJ; 39 pages, 16 figures, 1 tabl

Percutaneous combined therapy for painful sternal metastases: a radiofrequency thermal ablation (RFTA) and cementoplasty protocol

Radiofrequency thermal ablation (RFTA) has recently been introduced for the treatment of painful bone metastases. We report the outcome of one combined protocol session of percutaneous RFTA and cementoplasty on a painful sternal breast cancer metastasis of a 66-year-old patient

New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis

Here is an updated report on the genomic organization of T cell receptor beta (TRB) locus in the domestic dog (Canis lupus familiaris) as inferred from comparative genomics and expression analysis. The most interesting results we found were a second TRBD–J–C cluster, which is absent from the reference genome sequence, and the annotation of two additional TRBV genes. In dogs, TRB locus consists of a library of 37 TRBV genes positioned at the 50 end of two in tandem aligned D–J–C gene clusters, each composed of a single TRBD, 6 TRBJ and one TRBC genes, followed by a single TRBV gene with an inverted transcriptional orientation. The TRB genes are distributed in less than 300 kb, making the canine locus, one of the smaller mammalian TRB locus studied so far. The small size may be ascribed to reduced gene duplication occurrences and a lower density of total interspersed repeats compared to humans and mice. Despite the low TRBV gene content, a large and diversified beta chain repertoire is displayed in the dog peripheral blood. A full usage of TRBV and TRBJ genes, including pseudogenes, and a high level of allelic polymorphism contribute to generate diversity. Finally, this study suggests that the overall TRB locus organization is evolutionarily conserved supporting the dog as a highly suited model system for immune development and diseases

The chemical composition of the low-mass Galactic globular cluster NGC 6362

We present chemical abundances for 17 elements in a sample of 11 red giant branch stars in NGC 6362 from UVES spectra. NGC 6362 is one of the least massive globulars where multiple populations have been detected, yet its detailed chemical composition has not been investigated so far. NGC 6362 turns out to be a metal-intermediate ([Fe/H]=-1.07\pm0.01 dex) cluster, with its \alpha- and Fe-peak elements content compatible with that observed in clusters with similar metallicity. It also displays an enhancement in its s-process element abundances. Among the light elements involved in the multiple populations phenomenon, only [Na/Fe] shows star-to-star variations, while [Al/Fe] and [Mg/Fe] do not show any evidence for abundance spreads. A differential comparison with M4, a globular cluster with similar mass and metallicity, reveals that the two clusters share the same chemical composition. This finding suggests that NGC 6362 is indeed a regular cluster, formed from gas that has experienced the same chemical enrichment of other clusters with similar metallicity

Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis

The bacterial PorB porin, an ATP-binding beta-barrel protein of pathogenic Neisseria gonorrhoeae, triggers host cell apoptosis by an unknown mechanism. PorB is targeted to and imported by host cell mitochondria, causing the breakdown of the mitochondrial membrane potential (delta psi m). Here, we show that PorB induces the condensation of the mitochondrial matrix and the loss of cristae structures, sensitizing cells to the induction of apoptosis via signaling pathways activated by BH3-only proteins. PorB is imported into mitochondria through the general translocase TOM but, unexpectedly, is not recognized by the SAM sorting machinery, usually required for the assembly of beta-barrel proteins in the mitochondrial outer membrane. PorB integrates into the mitochondrial inner membrane, leading to the breakdown of delta psi m. The PorB channel is regulated by nucleotides and an isogenic PorB mutant defective in ATP-binding failed to induce delta psi m loss and apoptosis, demonstrating that dissipation of delta psi m is a requirement for cell death caused by neisserial infection

The absolute age of the globular cluster M15 using near-infrared adaptive optics images from PISCES/LBT

We present deep near-infrared (NIR) J, Ks photometry of the old, metal-poor Galactic globular cluster M\,15 obtained with images collected with the LUCI1 and PISCES cameras available at the Large Binocular Telescope (LBT). We show how the use of First Light Adaptive Optics system coupled with the (FLAO) PISCES camera allows us to improve the limiting magnitude by ~2 mag in Ks. By analyzing archival HST data, we demonstrate that the quality of the LBT/PISCES color magnitude diagram is fully comparable with analogous space-based data. The smaller field of view is balanced by the shorter exposure time required to reach a similar photometric limit. We investigated the absolute age of M\,15 by means of two methods: i) by determining the age from the position of the main sequence turn-off; and ii) by the magnitude difference between the MSTO and the well-defined knee detected along the faint portion of the MS. We derive consistent values of the absolute age of M15, that is 12.9+-2.6 Gyr and 13.3+-1.1 Gyr, respectively.Comment: 13 pages, 8 figures, ApJ accepte