Early postpartum luteal function after treatment with progestin and(or) gonadotropin-releasing hormone in dairy cattle

Progestin (Norgestomet®) and(or) repeated low-dose infusions of GnRH (Cystorelin@) influenced the lifespan of the first corpus luteum after an induced ovulation. Holstein cows (n=32) were assigned at calving to four groups. Cows were treated with blank ear implants (days 2 to 9 after calving) and saline infusion (48 hr on days 10 arid II), progestin ear implants and saline infusion, blank implants and GnRH infusion, or progestin implants and GnRH infusion prior to a GnRH-induced ovulation (day 12). Four primiparous and four multiparous cows were assigned to each treatment. Fewer cows treated with progestin/GnRH ovulated in response to the GnRH challenge. However, short cycles (<17 days in duration) were prevented in all cows (n= 16) treated with progestin. In addition, all multiparous cows treated with blank implants and GnRH infusion had normal cycles. Results of this study suggested that progestin and GnRH may have altered follicular development, thereby preventing the short-lived corpus luteum and inducing a normal estrous cycle as cows overcame anestrus early postpartum

The effect of Holstein-Friesian genotype and feeding system on selected performance parameters of dairy cows on grass-based systems of milk production in Ireland

End of project reportThe overall objective of this project was to assess, the effect of strain of Holstein-Friesian dairy cow, pasture-based feed system (FS) and their interaction on animal performance in terms of milk productivity and lactation profile, body weight (BW), body condition score (BCS), feed intake and energy balance (EB), reproductive performance and overall economic profitability

Role of differentially expressed microRNA-139-5p in the regulation of phenotypic internal anal sphincter smooth muscle tone.

The present study focused on the role of microRNA-139-5p (miRNA-139-5p) in the regulation of basal tone in internal anal sphincter (IAS). Applying genome-wide miRNA microarrays on the phenotypically distinct smooth muscle cells (SMCs) within the rat anorectrum, we identified miRNA-139-5p as differentially expressed RNA repressor with highest expression in the purely phasic smooth muscle of anococcygeus (ASM) vs. the truly tonic smooth muscle of IAS. This pattern of miRNA-139-5p expression, previously shown to target ROCK2, was validated by target prediction using ingenuity pathway (IPA) and by qPCR analyses. Immunoblotting, immunocytochemistry (ICC), and functional assays using IAS tissues and cells subjected to overexpression/knockdown of miRNA-139-5p confirmed the inverse relationship between miRNA-139-5p and ROCK2 expressions/IAS tone. Overexpression of miRNA-139-5p caused a decrease, while knockdown by anti-miRNA-139-5p caused an increase in the IAS tone; these tissue contractile responses were confirmed by single-cell contraction using magnetic twisting cytometry (MTC). These findings suggest miRNA-139-5p is capable of significantly influencing the phenotypic tonicity in smooth muscle via ROCK2: a lack of tone in ASM may be associated with the suppression of ROCK2 by high expression of miRNA-139-5p, whereas basal IAS tone may be associated with the persistence of ROCK2 due to low expression of miRNA-139-5p

Pregnancy rates of dairy cows at first service: influence of gonadotropin-releasing hormone and timing of AI relative to estrus

The 1989 Annual KSU Dairy Day is known as Dairy Day, 1989We demonstrated that gonadotropin-releasing hormone (GnRH or Cystorelin®) failed to improve pregnancy rates at the first service. When GnRH injection and insemination are both carried out either in early or late estrus or if cows are bred in early estrus and given a GnRH injection later in estrus, pregnancy rates are reduced by 9 to 13 percentage points compared to breeding according to the am-pm rule without GnRH treatment (control). Pregnancy rates of cows injected with GnRH early in estrus and bred in late estrus were similar to controls injected with saline and inseminated late in estrus (46 vs 43%). Altering the time of breeding and the time of GnRH injection to either early or late estrus did not improve pregnancy rates. We continue to recommend using GnRH only for repeat breeders, because GnRH consistently improves pregnancy rates at 3rd or 4th service, but not at first services

Mapping giant magnetic fields around dense solid plasmas by high resolution magneto-optical microscopy

We investigate distribution of magnetic fields around dense solid plasmas generated by intense p-polarized laser (~10^{16} W.cm^{-2}, 100 fs) irradiation of magnetic tapes, using high sensitivity magneto optical microscopy. We present evidence for giant axial magnetic fields and map out for the first time the spatial distribution of these fields. By using the axial magnetic field distribution as a diagnostic tool we uncover evidence for angular momentum associated with the plasma. We believe this study holds significance for investigating the process under which a magnetic material magnetizes or demagnetizes under the influence of ultrashort intense laser pulses.Comment: 17 pages of text with 4 figure

Using the 'myVolcano' mobile phone app for citizen science in St. Vincent and the Grenadines : a pilot study

The British Geological Survey (BGS) has been working with Caribbean partners on the role of citizen science in increasing resilience to natural hazards. The work has largely focused on the potential use of the myVolcano smartphone app, which was developed by the BGS following the 2010 Eyafjallajökull and 2011 Grímsvötn eruptions in Iceland. During these eruptions the BGS asked the UK public to collect particle samples, subsequently analysing these for ash presence to map the distribution of ash fallout across the UK. These requests led to the development of the myVolcano app, which was designed to capture transboundary and distal observations of volcanic ash and emissions. The observations are made visible to other users via an interactive map built into the app. The map interface has global coverage and the data collection methods (free-text descriptions and photographs) are such that information about any natural hazard, anywhere in the world, can be captured. In 2015, BGS carried out an ESRC-DfID-NERC funded scoping study in collaboration with the University of the West Indies’ Seismic Research Centre (UWI SRC), to test the potential use of the app in environments affected by proximal volcanic hazards. The study focused on St. Vincent and the Grenadines and investigated the potential for capturing a wider variety of observations for use by the public, operational scientists and civil protection. The study, which included a combination of desk study and remote interviews, highlighted the potential for, and challenges of, using such an app for increasing resilience to natural hazards and the need for a follow-up study in St Vincent. In March 2017, a workshop and school activities were held in St. Vincent to collect feedback from potential users of myVolcano, hereafter referred to as the pilot study. Workshop participants came from across government, monitoring agencies, emergency response and telecommunications. As part of the workshop, a multi-hazard scenario was ‘played out’ to stimulate discussions on the usability of the app, data gathering and processing, and participants’ use of existing citizen science applications. Discussions developed around data validation and quality assurance, data sharing and presentation, local management of data by nominated scientists (e.g. to facilitate real-time decision making) and the associated need for a locally appropriate app (i.e. no one size fits all). This last point is particularly significant when considering the utility of an app in several countries – the user interface, at least, requires specific tailoring to the country’s needs. Using this feedback, the BGS Official Development Assistance (ODA) programme is currently funding collaborations with Caribbean partners in order to modify the app to meet the local requirements, including widening the multi-hazard application and enhancing two-way information sharing. Of particular importance is how best to share critical information with those making observations and how to make observations available to decision-makers and monitoring scientists in real-time (e.g. through local management of the app)

Current Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Elderly French People: Troublesome Clones on the Horizon.

In 2015, we conducted at 44 healthcare facilities (HCFs) and 21 nursing homes (NHs) a 3-month bloodstream infection (BSI) survey, and a 1-day prevalence study to determine the rate of carriage of methicillin-resistant Staphylococcus aureus (MRSA) in 891 patients and 470 residents. We investigated the molecular characteristics of the BSI-associated and colonizing MRSA isolates, and assessed cross-transmission using double-locus sequence typing and pulsed-field gel electrophoresis protocol. The incidence of MRSA-BSI was 0.040/1000 patient-days (19 cases). The prevalence of MRSA carriage was 4.2% in patients (n = 39) and 8.7% in residents (n = 41) (p &lt; 0.001). BSI-associated and colonizing isolates were similar: none were PVL-positive; 86.9% belonged to clonal complexes 5 and 8; 93.9% were resistant to fluoroquinolones. The qacA/B gene was carried by 15.8% of the BSI-associated isolates [3/3 BSI cases in intensive care units (ICUs)], and 7.7% of the colonizing isolates in HCFs. Probable resident-to-resident transmission was identified in four NHs. Despite generally reassuring results, we identified two key concerns. First, a worryingly high prevalence of the qacA/B gene in MRSA isolates. Antisepsis measures being crucial to prevent healthcare-associated infections, our findings raise questions about the potential risk associated with chlorhexidine use in qacA/B(+) MRSA carriers, particularly in ICUs. Second, NHs are a weak link in MRSA control. MRSA spread was not controlled at several NHs; because of their frequent contact with the community, conditions are favorable for these NHs to serve as reservoirs of USA300 clone for local HCFs

Numerical investigations of flow and energy fields near a thermoacoustic couple

The flow field and the energy transport near thermoacoustic couples are simulated using a 2D full Navier-Stokes solver. The thermoacoustic couple plate is maintained at a constant temperature; plate lengths, which are short and long compared with the particle displacement lengths of the acoustic standing waves, are tested. Also investigated are the effects of plate spacing and the amplitude of the standing wave. Results are examined in the form of energy vectors, particle paths, and overall entropy generation rates. These show that a net heat-pumping effect appears only near the edges of thermoacoustic couple plates, within about a particle displacement distance from the ends. A heat-pumping effect can be seen even on the shortest plates tested when the plate spacing exceeds the thermal penetration depth. It is observed that energy dissipation near the plate increases quadratically as the plate spacing is reduced. The results also indicate that there may be a larger scale vortical motion outside the plates which disappears as the plate spacing is reduced. (C) 2002 Acoustical Society of America

The effects of altering milking frequency and/or diet in early lactation on the energy balance, production and reproduction of dairy cows.

End of Project ReportIt has been suggested that negative energy balance (NEB) in the immediate post-partum period is potentially an important factor in the association between increasing milk output and declining reproductive performance. The objective of this project was to design an experimental model that could be used to impose different degrees of NEB immediately after calving and to examine the effect of this model on dry matter intake (DMI), milk production, energy balance (EB), metabolic and reproductive hormonal profiles, the onset and pattern of post-partum ovarian cyclicity and reproductive physiology around AI. Two experiments were carried out to evaluate the effects of milking frequency and diet on DMI, production, energy balance and blood metabolites and hormones in the first 4 weeks after calving and subsequent reproduction. Reducing milking frequency from either thrice or twice daily to once daily reduced DMI but also reduced milk production. This resulted in a better EB in once daily milked cows in both experiments, the reduction being significant in the first. Milk production during the 4-week treatment period was reduced by 23 and 20 percent by reducing milking frequency from thrice to once daily in experiments 1 and 2, respectively. There was a reduction of approximately 10 percent in the cumulative yield up to week 20 of lactation in experiment 1 and of approximately 9 percent in total lactation yield in experiment 2. Reducing milking frequency resulted in increased plasma glucose, insulin and IGF-1 concentrations and reduced non-esterified fatty acid (NEFA) and beta hydroxybutyrate (BHB) concentrations. Conception rates to first service or overall pregnancy rates were not different between milking frequency treatments but once daily milking resulted in a shorter interval to first ovulation than thrice daily milking, due to a higher proportion of cows on this treatment ovulating the first post-partum dominant follicle. Increasing the energy density 2 of the diet increased DMI and milk production with no consequent effect on energy balance. Logistic regression on the combined data from the two experiments showed that lower energy intake, greater NEB and lower milk protein content and were significantly associated with poorer conception to first service. Lower plasma IGF-1 concentrations in experiment 2 were also associated with a lower conception rate to first service. A third experiment which investigated protein concentration in the concentrate combined with concentrate feeding level post calving (for two groups of cows in different body condition score at calving) showed no effect of post calving diet on BCS change. Overall the results suggest that reducing milking frequency to once per day during the first 4 weeks of lactation reduces NEB and appears to be a suitable strategy for altering energy balance at this time. However, the short-term reduction in milking frequency immediately post partum reduces total lactation yields. Blood metabolite and hormonal concentrations indicate better energy balance for cows milked once daily. Increasing dietary energy density or reducing the protein content of the diet does not appear to be effective in changing energy balance in early lactation. Decreased NEB in the first 4 weeks post-partum is associated with an improved conception rate to first service