IMPORTANCE OF GROWTH REGULATORE AND COLD STORAGE TREATMENTS FOR BREAKING OF GLADIOLUS (GLADIOLUS GRANDIFLORUS L.)CORM DORMANCY

The present experiment consisted of two genotypes viz., White Prosperity and Phule Neelrekha, four growth regulator treatments were GA3-125 ppm, BA- 50 ppm, NAA-100 ppm and control while five cold storage treatments viz., 6 weeks storage, 12 weeks storage, 18 weeks storage, 24 weeks storage and control. In the growth regulator GA3 125 ppm and in cold storage 24 week cold storage treatments was most effective in the breaking of dormancy and resulted in earlier sprouting. It concluded that Gladiolus corm dormancy will be break by the use of growth regulators and cold storage treatment

EVALUATION OF GENETIC VARIABILITY HERITABILITY AND GENETIC ADVANCES IN GLADIOLUS (GLADIOLUS GRANDIFLORUS L.) GENOTYPES

Assessment of variability, heritability and genetic advances of 31 gladiolus genotypes were evaluated. The presence of high amount of variability for all characters except sprout per corm and number of side spikes in the 31 gladiolus genotypes was recorded. The heritability of different field characters was categorized as very high which ranged between 23.40% (sprouts per corm) to 96.10% (florets per spike). Very high heritability coupled with high genetic advance for spike length, days to 50 per cent flowering, plant height advocated high genetic progress for them. It was concluded that highly significant varietal differences indicated the presence of high amount of variability

Formulation and In-Vitro Evaluation of Enteric Coated Tablet Incorporating Rabeprazole

The objective of the work is to try and assess the applicability and manufacturing possibilities to optimize an enteric coated tablet formulation containing Rabeprazole sodium as the drug aiming at the anti-acidity activity with desired drug release properties. Enteric coated tablet was chosen as dosage form being a cost-effective technology for pharmaceutical industry requiring fewer procedures. Before the implementation of the pharmaceutical technological aims, analysis of critical factors influencing the manufacture was carried out. Reproducible manufacturing processes are required to achieve suitability and tablets uniformity to achieve the uniform properties of tablets, which could influence experimental parameters. Rabeprazole in core content of tablet is blended with HPMC (different grades), xanthan gum, PVPK30, mannitol, crosspovidone, Sodium starch glycolate, Colloidal silicon dioxide to formulate the product. Prepared formulation was tested for weight and content uniformity, physical characteristics, in vitro dissolution behaviour, acid resistance and accelerated stability studies. All studies performed resulted and revealed for assurance of such enteric coated tablet formulation for drug Rabeprazole with optimum characteristics, concluding it as a promising approach to enhance drug release characteristics. Keywords: Rabeprazole, HPMC, enteric coated tablets, In Vitro evaluation

Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana)

Infectious diseases remain a significant threat to human health, contributing to more than 17 million deaths, annually. With the worsening trends of drug resistance, there is a need for newer and more powerful antimicrobial agents. We hypothesized that animals living in polluted environments are potential source of antimicrobials. Under polluted milieus, organisms such as cockroaches encounter different types of microbes, including superbugs. Such creatures survive the onslaught of superbugs and are able to ward off disease by producing antimicrobial substances. Here, we characterized antibacterial properties in extracts of various body organs of cockroaches (Periplaneta americana) and showed potent antibacterial activity in crude brain extract against methicillin-resistant Staphylococcus aureus and neuropathogenic E. coli K1. The size-exclusion spin columns revealed that the active compound(s) are less than 10 kDa in molecular mass. Using cytotoxicity assays, it was observed that pre-treatment of bacteria with lysates inhibited bacteria-mediated host cell cytotoxicity. Using spectra obtained with LC-MS on Agilent 1290 infinity liquid chromatograph, coupled with an Agilent 6460 triple quadruple mass spectrometer, tissues lysates were analyzed. Among hundreds of compounds, only a few homologous compounds were identified that contained isoquinoline group, chromene derivatives, thiazine groups, imidazoles, pyrrole containing analogs, sulfonamides, furanones, flavanones, and known to possess broad-spectrum antimicrobial properties, and possess anti-inflammatory, anti-tumour, and analgesic properties. Further identification, characterization and functional studies using individual compounds can act as a breakthrough in developing novel therapeutics against various pathogens including superbugs

Egg retention and dispersal activity in the parasitoid wasp, Trichogramma principium

Effects of egg retention on movement and dispersal activity of Trichogramma principium (Hymenoptera, Trichogrammatidae) females were investigated under laboratory conditions. Individual females were observed during one minute in the absence of hosts. Movement activity and dispersal rate were estimated by the length of the track and by the distance from the start point, respectively. Before the test, all wasps during 2 – 4 days were presented with a possibility to parasitize a factitious laboratory host, Sitotroga cerealella Oliv. (Lepidoptera, Gelechiidae). Wasps that had parasitized before the test show significant reduction of spontaneous walking activity and dispersal rate when compared with females that refused to parasitize the non-preferred host (i.e. manifested egg retention). This effect cannot be considered as a direct arrestment reaction to the host because during the test period, no hosts were provided. Thus, egg retention results not only in temporal spread, but also in more intensive spatial dispersal of a group of simultaneously emerged females

Embryo rescue: A tool to overcome incompatible interspecific hybridization in <i style="">Gossypium</i> Linn.-<b style=""></b>A review

29-36Successful introgression of desirable characters from certain species could not be achieved because of different incompatibility reasons. Such incompatibility can be overcome by ovulo-embryo culture. From the literature reviewed so far, it appears that the technique involving excision of 10 to 15-day-old (after pollination) embryos and their subsequent culture on different combinations of BT and MS media was successful in obtaining difficult interspecific hybrids in Gossypium spp. BT and MS media supplemented with phytohormones, casein hydrolysate and IAA were more effective for culturing interspecific hybrid embryos. Other factors, like age and genotype of embryo; basal media, temperature and photoperiod during culturing; and hardening of plants are also important in determining the success of the interspecific embryo culture in Gossypium spp