INFLUENCE OF ZINC OXIDE NANOPARTICLES ON GROWTH OF SESAMUM INDICUM L. IN ZINC DEFICIENT SOIL

Objective: A comparative examine of L. aculeate mediated ZnO (BZnO) and synthesized chemical ZnO (CZnO) nanoparticles became done which will decide the impact of seed germination on Sesamum indicum (CO-1). Methods: Zinc oxide nanoparticles had been synthesized by the biological and chemical methods. Synthesized nanoparticles have been confirmed with Ultra Violet–visible spectroscopy (UV-Vis), Fourier transform infrared spectrometer (FT-IR), Energy dispersive X-ray spectrometer (EDX), X-ray diffractometer (XRD), Field Emission Scanning Electron Microscopy (FESEM) and High-Resolution Transmission Electron Microscopy (HRTEM). Different concentration of synthesized zinc oxide nanoparticles (0.1, 0.25, 0.5, 1 and 2 g/l) solution were prepared and applied to Sesamum indicum by soaking approach. The impact of ZnO nanoparticles treated plants were studied by using the standard procedure on plant growth attributes (shoot and root length, dry and fresh weight of shoot and root), photosynthesis pigment (chlorophyll a, chlorophyll b and total chlorophyll) and biochemical analysis (protein, carbohydrate and reducing sugar). Results: The characterization analysis revealed that BZnO & CZnO NPs were spherical in shape with a mean particle size of 12±3 nm and 18±2 nm. The maximum observation of growth attributes was recorded in 0.5 g/l concentration of biologically synthesized ZnO nanoparticles which was compared to chemically synthesized ZnO nanoparticles. Conclusion: Results of this experiment revealed that Lantana aculeate mediated zinc oxide nanoparticles are an enhanced useful resource of Sesamum indicum. It is able to use as nano fertilizer in agriculture development. Keywords: Plant growth attributes, Sesamum indicum, Seed germination, Zinc oxide nanoparticles

Synergistic effect of Fe and Co doped ZnO nanoparticles synthesized using Alpinia galanga against Candida parasilopsis

In this investigation, nanoparticles such as ZnO, Fe doped ZnO and Co doped ZnO NPs prepared by the co-precipitation method were tested against the pathogenic yeast. The spectroscopic analyses were carried out to identify the morphological and chemical composition of the synthesized nanoparticles. The results of XRD analysis revealed that the synthesized nanoparticles were crystalline in nature with average size ranges between 32 – 34 nm approximately. EDX and SEM analysis were carried out to identify the element composition (Co, Fe and Zn) and spherical shape of nanoparticles. The functional group that is responsible for the capping and stability of nanoparticles was confirmed by FTIR analysis,  to compare the antifungal efficiency of ZnO, Fe doped ZnO and Co doped ZnO from the resultant zone of inhibition

SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY

Objective: To assess the antioxidant and anticancer activity of crude ethyl acetate extract from Streptomyces cavouresis KU-V39 isolated from vermicompost. Methods: To determine its antioxidant activity by total phenol and flavonoid content, Hydrogen peroxide radical scavenging activity and Ferrous reducing power assay. To determine in vitro cytotoxicity, various concentrations of extract was tested on HeLa cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectroscopy (GC-MS) have been carried out to investigate the bioactive compound of crude extract. Results: The Phenol and flavonoid content of crude extract was found to be 20.24 and 26.60 mg /g metabolite. Isolate KU-V39 exhibited H2O2 scavenging activity at IC50 value is 42.35 ± 0.75 μg/ml. The ferrous reducing power assay has higher absorbance value which indicates the high antioxidant capacity of the extracts. The analysis of FT-IR spectroscopy indicate presents of Phenol, carboxylic acid, alkanes, nitro and aliphatic amines functional groups. The GC – MS analysis revealed that presence of Pentanoic acid, L-Proline, Lysine, Erucic acid, isosteviol, Pentadecanoic acid, Phthalic acid, Hexadecanoic acid, Octadecenoic acid, Dichloroacetic acid, Cyclohexanecarboxylic acid and Pyrrolo pyrazine derivative. Conclusion: This spectral study clearly proved that the vermicompost derived Streptomyces with extra cellular metabolism showed promising antioxidant and anticancer activity