Platelet kinetics in the pulmonary microcirculation in vivo assessed by intravital microscopy

Growing evidence supports the substantial pathophysiological impact of platelets on the development of acute lung injury. Methods for studying these cellular mechanisms in vivo are not present yet. The aim of this study was to develop a model enabling the quantitative analysis of platelet kinetics and platelet-endothelium interaction within consecutive segments of the pulmonary microcirculation in vivo. New Zealand White rabbits were anesthetized and ventilated. Autologous platelets were separated from blood and labeled ex vivo with rhodamine 6G. After implantation of a thoracic window, microhemodynamics and kinetics of platelets were investigated by intravital microscopy. Velocities of red blood cells (RBCs) and platelets were measured in arterioles, capillaries and venules, and the number of platelets adhering to the microvascular endothelium was counted. Kinetics of unstimulated platelets was compared with kinetics of thrombin-activated platelets. Velocity of unstimulated platelets was comparable to RBC velocity in all vessel segments. Unstimulated platelets passed the pulmonary microcirculation without substantial platelet-endothelial interaction. In contrast, velocity of activated platelets was decreased in all vascular segments indicating platelet margination and temporal platelet-endothelium interaction. Thrombin-activated platelets adhered to arteriolar endothelium; in capillaries and venules adherence of platelets was increased 8-fold and 13-fold, respectively. In conclusion, using intravital microscopy platelet kinetics were directly analyzed in the pulmonary microcirculation in vivo for the first time. In contrast to leukocytes, no substantial platelet-endothelium interaction occurs in the pulmonary microcirculation without any further stimulus. In response to platelet activation, molecular mechanisms enable adhesion of platelets in arterioles and venules as well as retention of platelets within capillaries. Copyright (C) 2002 S. Karger AG, Basel

High yield fusion in a Staged Z-pinch

We simulate fusion in a Z-pinch; where the load is a xenon-plasma liner imploding onto a deuterium-tritium plasma target and the driver is a 2 MJ, 17 MA, 95 ns risetime pulser. The implosion system is modeled using the dynamic, 2-1/2 D, radiation-MHD code, MACH2. During implosion a shock forms in the Xe liner, transporting current and energy radially inward. After collision with the DT, a secondary shock forms pre-heating the DT to several hundred eV. Adiabatic compression leads subsequently to a fusion burn, as the target is surrounded by a flux-compressed, intense, azimuthal-magnetic field. The intense-magnetic field confines fusion $\alpha$-particles, providing an additional source of ion heating that leads to target ignition. The target remains stable up to the time of ignition. Predictions are for a neutron yield of $3.0\times 10^{19}$ and a thermonuclear energy of 84 MJ, that is, 42 times greater than the initial, capacitor-stored energy

Large deviations for a damped telegraph process

In this paper we consider a slight generalization of the damped telegraph process in Di Crescenzo and Martinucci (2010). We prove a large deviation principle for this process and an asymptotic result for its level crossing probabilities (as the level goes to infinity). Finally we compare our results with the analogous well-known results for the standard telegraph process

Observations and simulations of recurrent novae: U Sco and V394 CrA

Observations and analysis of the Aug. 1987 outburst of the recurrent nova V394 CrA are presented. This nova is extremely fast and its outburst characteristics closely resemble those of the recurrent nova U Sco. Hydrodynamic simulations of the outbursts of recurrent novae were performed. Results as applied to the outbursts of V394 CrA and U Sco are summarized

All Politics is Local: The Renminbi's Prospects as a Future Global Currency

. In this article we describe methods for improving the RWTH German speech recognizer used within the VERBMOBIL project. In particular, we present acceleration methods for the search based on both within-word and across-word phoneme models. We also study incremental methods to reduce the response time of the online speech recognizer. Finally, we present experimental off-line results for the three VERBMOBIL scenarios. We report on word error rates and real-time factors for both speaker independent and speaker dependent recognition. 1 Introduction The goal of the VERBMOBIL project is to develop a speech-to-speech translation system that performs close to real-time. In this system, speech recognition is followed by subsequent VERBMOBIL modules (like syntactic analysis and translation) which depend on the recognition result. Therefore, in this application it is particularly important to keep the recognition time as short as possible. There are VERBMOBIL modules which are capable to work ..

Structural, chemical and magnetic properties of secondary phases in Co-doped ZnO

We have utilized a comprehensive set of experimental techniques such as transmission electron microscopy (TEM) and synchrotron-based x-ray absorption spectroscopy (XAS) and the respective x-ray linear dichroism and x-ray magnetic circular dichroism to characterize the correlation of structural, chemical and magnetic properties of Co-doped ZnO samples. It can be established on a quantitative basis that the superparamagnetic (SPM) behavior observed by integral superconducting quantum interference device magnetometry is not an intrinsic property of the material but stems from precipitations of metallic Co. Their presence is revealed by TEM as well as XAS. Annealing procedures for these SPM samples were also studied, and the observed changes in the magnetic properties found to be due to a chemical reduction or oxidation of the metallic Co species

Chaos and Universality in a Four-Dimensional Spin Glass

We present a finite size scaling analysis of Monte Carlo simulation results on a four dimensional Ising spin glass. We study chaos with both coupling and temperature perturbations, and find the same chaos exponent in each case. Chaos is investigated both at the critical temperature and below where it seems to be more efficient (larger exponent). Dimension four seems to be above the critical dimension where chaos with temperature is no more present in the critical region. Our results are consistent with the Gaussian and bimodal coupling distributions being in the same universality class.Comment: 11 pages, including 6 postscript figures. Latex with revtex macro

Fractal Droplets in Two Dimensional Spin Glasses

The two-dimensional Edwards-Anderson model with Gaussian bond distribution is investigated at T=0 with a numerical method. Droplet excitations are directly observed. It turns out that the averaged volume of droplets is proportional to l^D with D = 1.80(2) where l is the spanning length of droplets, revealing their fractal nature. The exponent characterizing the l dependence of the droplet excitation energy is estimated to be -0.42(4), clearly different from the stiffness exponent for domain wall excitations.Comment: 4 pages 4 figure

Study of Chirality in the Two-Dimensional XY Spin Glass

We study the chirality in the Villain form of the XY spin glass in two--dimensions by Monte Carlo simulations. We calculate the chiral-glass correlation length exponent $\nu_{\scriptscriptstyle CG}$ and find that $\nu_{\scriptscriptstyle CG} = 1.8 \pm 0.3$ in reasonable agreement with earlier studies. This indicates that the chiral and phase variables are decoupled on long length scales and diverge as $T \to 0$ with {\em different} exponents, since the spin-glass correlation length exponent was found, in earlier studies, to be about 1.0.Comment: 4 pages. Latex file and 4 embedded postscript files are included in a self-unpacking compressed tar file. A postscript version is available at ftp://chopin.ucsc.edu/pub/xysg.p

Chaos in a Two-Dimensional Ising Spin Glass

We study chaos in a two dimensional Ising spin glass by finite temperature Monte Carlo simulations. We are able to detect chaos with respect to temperature changes as well as chaos with respect to changing the bonds, and find that the chaos exponents for these two cases are equal. Our value for the exponent appears to be consistent with that obtained in studies at zero temperature.Comment: 4 pages, LaTeX, 4 postscript figures included. The analysis of the data is now done somewhat differently. The results are consistent with the chaos exponent found at zero temperature. Additional papers of PY can be obtained on-line at http://schubert.ucsc.edu/pete