Spectral Analysis of Correlated One-Dimensional Systems with Impurities

An averaging procedure is proposed to account for spectral features of correlated one-dimensional systems in the presence of non-magnetic impurities. The dynamical spin structure factor for a corresponding random ensemble of Heisenberg chain segments is calculated by exact numerical diagonalization. It is shown that a few-pole approximation is sufficient to describe the numerical results. A similar analysis is proposed for the discussion of experimental spectra, such as obtained by inelastic neutron scattering measurements on Zn-doped CuO chains. By examination of the disorder-induced pseudo-gap, the loss of spectral weight, and the discrete peak structures due to smallest-cluster contributions, the underlying impurity distribution function can be determined.Comment: RevTex, 4 pages with 4 eps figure

Ensuring the safety of animal feed

A general outline is presented of measures for the production of safe animal feed. This is based on the setting of so-called ‘feed safety objectives’ which make use of principles that relate to animal health, animal welfare, legal aspects of farm practices and human food safety objectives for products of animal origin. Particular emphasis will be put on the types of feed used in relation to feedborne animal diseases caused by infectious and chemical agents and on the relationship between animal feed and zoonotic foodborne diseases. In addition the influence of feed on animal welfare will be discussed. To produce safe animal feed, a pro-active control system is advocated. This approach has been very successful in relation to human food and involves the use of ‘good manufacturing practices’ (GMP) and the ‘hazard analysis critical control point’ (HACCP) concept as the main tools. However, it has been shown that the HACCP-system has certain shortcomings. To counteract these shortcomings, product traceability and hazard early-warning systems have been developed and will also be presented

An Enzyme-Linked Immunosorbent Spot Assay Measuring Borrelia burgdorferi B31-Specific Interferon Gamma-Secreting T Cells Cannot Discriminate Active Lyme Neuroborreliosis from Past Lyme Borreliosis: a Prospective Study in the Netherlands.

Two-tier serology testing is most frequently used for the diagnosis of Lyme borreliosis (LB); however, a positive result is no proof of active disease. To establish a diagnosis of active LB, better diagnostics are needed. Tests investigating the cellular immune system are available, but studies evaluating the utility of these tests on well-defined patient populations are lacking. Therefore, we investigated the utility of an enzyme-linked immunosorbent spot (ELISpot) assay to diagnose active Lyme neuroborreliosis. Peripheral blood mononuclear cells (PBMCs) of various study groups were stimulated by using Borrelia burgdorferi strain B31 and various recombinant antigens, and subsequently, the number of Borrelia-specific interferon gamma (IFN-γ)-secreting T cells was measured. We included 33 active and 37 treated Lyme neuroborreliosis patients, 28 healthy individuals treated for an early manifestation of LB in the past, and 145 untreated healthy individuals. The median numbers of B. burgdorferi B31-specific IFN-γ-secreting T cells/2.5 × 105 PBMCs did not differ between active Lyme neuroborreliosis patients (6.0; interquartile range [IQR], 0.5 to 14.0), treated Lyme neuroborreliosis patients (4.5; IQR, 2.0 to 18.6), and treated healthy individuals (7.4; IQR, 2.3 to 14.9) (P = 1.000); however, the median number of B. burgdorferi B31-specific IFN-γ-secreting T cells/2.5 × 105 PBMCs among untreated healthy individuals was lower (2.0; IQR, 0.5 to 3.9) (P ≤ 0.016). We conclude that the Borrelia ELISpot assay, measuring the number of B. burgdorferi B31-specific IFN-γ-secreting T cells/2.5 × 105 PBMCs, correlates with exposure to the Borrelia bacterium but cannot be used for the diagnosis of active Lyme neuroborreliosis

Placental syncytiotrophoblast constitutes a major barrier to vertical transmission of Listeria monocytogenes.

Listeria monocytogenes is an important cause of maternal-fetal infections and serves as a model organism to study these important but poorly understood events. L. monocytogenes can infect non-phagocytic cells by two means: direct invasion and cell-to-cell spread. The relative contribution of each method to placental infection is controversial, as is the anatomical site of invasion. Here, we report for the first time the use of first trimester placental organ cultures to quantitatively analyze L. monocytogenes infection of the human placenta. Contrary to previous reports, we found that the syncytiotrophoblast, which constitutes most of the placental surface and is bathed in maternal blood, was highly resistant to L. monocytogenes infection by either internalin-mediated invasion or cell-to-cell spread. Instead, extravillous cytotrophoblasts-which anchor the placenta in the decidua (uterine lining) and abundantly express E-cadherin-served as the primary portal of entry for L. monocytogenes from both extracellular and intracellular compartments. Subsequent bacterial dissemination to the villous stroma, where fetal capillaries are found, was hampered by further cellular and histological barriers. Our study suggests the placenta has evolved multiple mechanisms to resist pathogen infection, especially from maternal blood. These findings provide a novel explanation why almost all placental pathogens have intracellular life cycles: they may need maternal cells to reach the decidua and infect the placenta

National laboratory-based surveillance system for antimicrobial resistance: a successful tool to support the control of antimicrobial resistance in the Netherlands

An important cornerstone in the control of antimicrobial resistance (AMR) is a well-designed quantitative system for the surveillance of spread and temporal trends in AMR. Since 2008, the Dutch national AMR surveillance system, based on routine data from medical microbiological laboratories (MMLs), has developed into a successful tool to support the control of AMR in the Netherlands. It provides background information for policy making in public health and healthcare services, supports development of empirical antibiotic therapy guidelines and facilitates in-depth research. In addition, participation of the MMLs in the national AMR surveillance network has contributed to sharing of knowledge and quality improvement. A future improvement will be the implementation of a new semantic standard together with standardised data transfer, which will reduce errors in data handling and enable a more real-time surveillance. Furthermore, the

Proximal Interphalangeal Joint Arthroplasty for Osteoarthritis:A Long-Term Follow-Up

Background: Previously published research describes short-term outcomes after proximal interphalangeal (PIP) joint arthroplasty; however, long-term outcomes are scarce. Therefore, the authors evaluated patient-reported outcomes and complications after a follow-up of at least 5 years following PIP joint arthroplasty. Methods: The authors used prospectively gathered data from patients undergoing PIP joint arthroplasty with silicone or surface replacement implants. Time points included preoperatively, 1 year postoperatively, and at least 5 years postoperatively. The authors were able to include 74 patients. Primarily, the authors focused on patient satisfaction with the treatment outcome, measured using a validated 5-point Likert scale. Secondary outcomes included the questions of whether patients would undergo the same surgery again, the assessment of factors associated with satisfaction or dissatisfaction, the Michigan Hand Outcomes Questionnaire score, and the number of reoperations. Results: The mean follow-up was 7 ± 1.2 years (range, 5 to 11 years). Patient satisfaction was excellent in 14 patients (19%), good in 17 patients (23%), reasonable in 18 patients (24%), moderate in 10 patients (14%), and poor in 15 patients (20%). Seventy-three percent of patients (n = 54) would undergo the same procedure again. The authors found no factors associated with satisfaction or dissatisfaction. All Michigan Hand Outcomes Questionnaire scores improved significantly in the first year after surgery and did not deteriorate afterward. Sixteen fingers (16%) required a reoperation, of which 3 (4%) needed a prosthesis replacement. Conclusions: Patient satisfaction with treatment outcomes 7 years after PIP implant surgery ranges from moderate to good for many patients, with a notable proportion expressing dissatisfaction. Patient-reported outcomes improve primarily within the first year and remain stable at 5 years or more.</p

Performance predictions for a laser intensified thermal beam for use in high resolution Focused Ion Beam instruments

Photo-ionization of a laser-cooled and compressed atomic beam from a high-flux thermal source can be used to create a high-brightness ion beam for use in Focus Ion Beam (FIB) instruments. Here we show using calculations and Doppler cooling simulations that an atomic rubidium beam with a brightness of $2.1 \times 10^7 A/(m^2\,sr\,eV)$ at a current of 1 nA can be created using a compact 5 cm long 2D magneto-optical compressor which is more than an order of magnitude better than the current state of the art Liquid Metal Ion Source.Comment: 8 pages, 7 figures submitted to: Phys. Rev.

Precision spectroscopy of helium in a magic wavelength optical dipole trap

Improvements in both theory and frequency metrology of few-electron systems such as hydrogen and helium have enabled increasingly sensitive tests of quantum electrodynamics (QED), as well as ever more accurate determinations of fundamental constants and the size of the nucleus. At the same time advances in cooling and trapping of neutral atoms have revolutionized the development of increasingly accurate atomic clocks. Here, we combine these fields to reach the highest precision on an optical tranistion in the helium atom to date by employing a Bose-Einstein condensate confined in a magic wavelength optical dipole trap. The measured transition accurately connects the ortho- and parastates of helium and constitutes a stringent test of QED theory. In addition we test polarizability calculations and ultracold scattering properties of the helium atom. Finally, our measurement probes the size of the nucleus at a level exceeding the projected accuracy of muonic helium measurements currently being performed in the context of the proton radius puzzle

Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults

BACKGROUND: Untreated human immunodeficiency virus (HIV) disease disrupts B cell populations causing reduced memory and reduced naïve resting B cells leading to increases in specific co-infections and impaired responses to vaccines. To what extent antiretroviral treatment reverses these changes in an African population is uncertain. METHODS: A cross-sectional study was performed. We recruited HIV-uninfected and HIV-infected Malawian adults both on and off antiretroviral therapy attending the Queen Elizabeth Central hospital in Malawi. Using flow cytometry, we enumerated B cells and characterized memory B cells and compared these measurements by the different recruitment groups. RESULTS: Overall 64 participants were recruited - 20 HIV uninfected (HIV-), 30 HIV infected ART naïve (HIV+N) and 14 HIV-infected ART treated (HIV+T). ART treatment had been taken for a median of 33 months (Range 12-60 months). Compared to HIV- the HIV+N adults had low absolute number of naïve resting B cells (111 vs. 180 cells/μl p = 0.008); reduced memory B cells (27 vs. 51 cells/μl p = 0.0008). The HIV+T adults had B-cell numbers similar to HIV- except for memory B cells that remained significantly lower (30 vs. 51 cells/μl p = 0.02). In the HIV+N group we did not find an association between CD4 count and B cell numbers. CONCLUSIONS: HIV infected Malawian adults have abnormal B-cell numbers. Individuals treated with ART show a return to normal in B-cell numbers but a persistent deficit in the memory subset is noted. This has important implications for long term susceptibility to co-infections and should be evaluated further in a larger cohort study