A bioinformatics approach to the development of immunoassays for specified risk material in canned meat products

A bioinformatics approach to developing antibodies to specific proteins has been evaluated for the production of antibodies to heat-processed specified risk tissues from ruminants (brain and eye tissue). The approach involved the identification of proteins specific to ruminant tissues by interrogation of the annotation fields within the Swissprot database. These protein sequences were then interrogated for peptide sequences that were unique to the protein. Peptides were selected that met these criteria as close as possible and that were also theoretically resistant to either pepsin or trypsin. The selected peptides were synthesised and used as immunogens to raise monoclonal antibodies. Antibodies specific for the synthetic peptides were raised to half of the selected peptides. These antibodies have each been incorporated into a competitive enzyme-linked immunosorbent assay (ELISA) and shown to be able to detect the heat-processed parent protein after digestion with either pepsin or trypsin. One antibody, specific for alpha crystallin peptide (from bovine eye tissue), was able to detect the peptide in canned meat products spiked with 10% eye tissue. These results, although preliminary in nature, show that bioinformatics in conjunction with enzyme digestion can be used to develop ELISA for proteins in high-temperature processed foods and demonstrate that the approach is worth further stud

Improving Predictions for Helium Emission Lines

We have combined the detailed He I recombination model of Smits with the collisional transitions of Sawey & Berrington in order to produce new accurate helium emissivities that include the effects of collisional excitation from both the 2 (3)S and 2 (1) S levels. We present a grid of emissivities for a range of temperature and densities along with analytical fits and error estimates. Fits accurate to within 1% are given for the emissivities of the brightest lines over a restricted range for estimates of primordial helium abundance. We characterize the analysis uncertainties associated with uncertainties in temperature, density, fitting functions, and input atomic data. We estimate that atomic data uncertainties alone may limit abundance estimates to an accuracy of 1.5%; systematic errors may be greater than this. This analysis uncertainty must be incorporated when attempting to make high accuracy estimates of the helium abundance. For example, in recent determinations of the primordial helium abundance, uncertainties in the input atomic data have been neglected.Comment: ApJ, accepte

Climbing the water ladder: multiple-use water services for poverty reduction

Multiple use / Models / Water productivity / Research projects / Water supply / Domestic water / Irrigation water / Water governance / Poverty / Gender / Rural areas / Wells / Water harvesting / Runoff / Water storage / Water purification / Appropriate technology / Costs / Local government / Non governmental organizations / Case studies / Ehiopia / Nepal / Zimbabwe / Bolivia / India / Colombia / Thailand / South Africa

Systematic effects and a new determination of the primordial abundance of 4He and dY/dZ from observations of blue compact galaxies

We use spectroscopic observations of a sample of 82 HII regions in 76 blue compact galaxies to determine the primordial helium abundance Yp and the slope dY/dZ from the Y-O/H linear regression. To improve the accuracy of the dY/dZ measurement, we have included new spectrophotometric observations of 33 HII regions which span a large metallicity range, with oxygen abundance 12+log(O/H) varying between 7.43 and 8.30 (Zsun/30<Z<Zsun/4). For a subsample of 7 HII regions, we derive the He mass fraction taking into account known systematic effects, including collisional and fluorescent enhancements of HeI emission lines, collisional excitation of hydrogen emission, underlying stellar HeI absorption and the difference between the temperatures Te(HeII) in the He^+ zone and Te(OIII) derived from the collisionally excited [OIII] lines. We find that the net result of all the systematic effects combined is small, changing the He mass fraction by less than 0.6%. By extrapolating the Y vs. O/H linear regression to O/H=0 for 7 HII regions of this subsample, we obtain Yp=0.2421+/-0.0021 and dY/dO=5.7+/-1.8, which corresponds to dY/dZ=3.7+/-1.2, assuming the oxygen mass fraction to be O=0.66Z. In the framework of the standard Big Bang nucleosynthesis theory, this Yp corresponds to Omega_b h^2 = 0.012^+0.003_-0.002, where h is the Hubble constant in units of 100 km/s/Mpc. This is smaller at the 2sigma level than the value obtained from recent deuterium abundance and microwave background radiation measurements. The linear regression slope dY/dO=4.3+/-0.7 (corresponding to dY/dZ=2.8+/-0.5) for the whole sample of 82 HII regions is similar to that derived for the subsample of 7 HII regions, although it has a considerably smaller uncertainty.Comment: 53 pages, 3 Postscript figures, accepted for publication in the Astrophysical Journa

A search for 4750- and 4765-MHz OH masers in Southern Star Forming Regions

We have used the Australia Telescope Compact Array (ATCA) to make a sensitive (5-$\sigma$ $\simeq$ 100 mJy) search for maser emission from the 4765-MHz $^2\Pi_{1/2}$ F=1$\to$0 transition of OH. Fifty five star formation regions were searched and maser emission with a peak flux density in excess of 100 mJy was detected toward fourteen sites, with ten of these being new discoveries. In addition we observed the 4750-MHz $^2\Pi_{1/2}$ F=1$\to$1 transition towards a sample of star formation regions known to contain 1720-MHz OH masers, detecting marginal maser emission from G348.550-0.979. If confirmed this would be only the second maser discovered from this transition. The occurrence of 4765-MHz OH maser emission accompanying 1720-MHz OH masers in a small number of well studied star formation regions has lead to a general perception in the literature that the two transitions favour similar physical conditions. Our search has found that the presence of the excited-state 6035-MHz OH transition is a much better predictor of 4765-MHz OH maser emission from the same region than is 1720-MHz OH maser emission. Combining our results with those of previous high resolution observations of other OH transitions we have examined the published theoretical models of OH masers and find that none of them predict any conditions in which the 1665-, 6035- and 4765-MHz transitions are simultaneously inverted. Erratum abstract: Dodson & Ellingsen (2002) included several observations with significant pointing errors, invalidating the upper limits found in these directions. These have now been reobserved or recalculated. A new table of upper limits has been generated, and two more masers that would have been seen have been found.Comment: Included an Erratum with Max as another author. This erratum was rejected by MNRAS (Feb 04) as it contained too much data. Resubmitted as a paper (Jun 04). Rejected (Sep 04) it had too little data. Resubmitted as reduced erratum (Apr 05). Still waitin

Flow in a Commercial Steel Pipe

Fully-developed turbulent flow in a commercial steel pipe is studied using single component hot-wire probes in both oneand two-point experiments. The streamwise turbulence component was measured over a Reynolds number range from 7.6× 104 to 8.3×106, covering the smooth to fully rough regimes. The experiments were conducted in the Princeton/ONR Superpipe facility that uses compressed air at pressures up to 200 atm as the working fluid. For Reynolds numbers less than about 8 ×105 the surface was hydraulically-smooth, and the results agreed closely with the smooth-wall turbulence intensity and spectral data obtained by Morrison et al. [10] and Zhao & Smits [14]. An assessment was performed of probe resolution and results indicate that the turbulence statistics of the large-scale motions were unaffected by the sensing wire length even at high Reynolds numbers. Transitionally-rough and fully-rough data showed deviation from the smooth-wall data as roughness effects became more prominent. In particular, the outer peak in the turbulence intensity observed at high Reynolds numbers in smooth pipe flow decreased in magnitude or stayed constant for transitionally rough and fully rough flow. The two-point azimuthal correlations were found to be consistent with the presence of very large scale coherent regions of low-wavenumber, low-momentum fluid observed in previous studies of wall-bounded flows. The correlations indicated that the azimuthal scale of these regions is Reynolds number independent

Constraining the properties of 1.2-mm dust clumps that contain luminous water masers

We have conducted a sensitive water maser search with the ATCA towards 267 1.2-mm dust clumps presented in the literature. We combine our new observations with previous water maser observations to extend our sample to 294 1.2-mm dust clumps, towards which we detect 165 distinct water maser sites towards 128 1.2-mm dust clumps. Within the fields of our observations, we additionally find four water masers with no apparent associated 1.2-mm dust continuum emission. Our overall detection rate of 44 per cent appears to vary as a function of Galactic longitude. We find that there is an excellent correspondence between the locations of the detected water masers with the peak of the target 1.2-mm dust clump sources. As expected from previous similar studies, the water masers are chiefly detected towards the bigger, brighter and more massive 1.2-mm dust clumps. We find further evidence that the water masers tend to increase in flux density (and therefore luminosity), as well as velocity range, as the sources evolve. We also show that the current sample of water maser sources suffer less from evolutionary biases than previous targeted searches. A higher fraction of dust clump sources in our sample are only associated with water masers (41) than only associated with methanol masers (13). This suggests that water masers can be present at an even earlier evolutionary stage than 6.7-GHz methanol masers. Comparison of the water maser detection rates associated with different combinations of methanol maser and radio continuum, as well as those with neither tracer, shows that the highest detection rate is towards those sources which also exhibit methanol maser emission. We have tested a previously hypothesised model for water maser presence towards 1.2-mm dust clumps. We suggest refinements and future work which will further constrain the nature of the driving sources associated with water masers.Comment: accepted to MNRA

The multiple-use water services (MUS) project

The CPWF-supported project ‘Models for implementing multiple-use water supply systems for enhanced land and water productivity, rural livelihoods and gender equity’ (‘CPWF-MUS’) innovated, tested, and documented homestead-scale and communityscale models for Multiple Use water Services in 30 rural and peri-urban sites in 8 countries: the Andes (Bolivia and Colombia), Indus-Ganges (India, Nepal), Limpopo (South Africa and Zimbabwe), Mekong (Thailand) and Nile (Ethiopia). Learning alliances for scaling up and out of results were forged in each country, encompassing a total of 150 water user groups, CBOs, (I)NGOs, domestic sub-sector and productive sub-sector agencies, local government, private service providers, rural development agencies and financers, and knowledge centers. The resulting institutional change at intermediate and national level, together with awareness raising about the MUS models at global level, contributed significantly to a more supportive environment for reaching all water users with the multiple-use water services they need and, thus, using water most effectively to achieve all MDG

The Chemical Composition of the Small Magellanic Cloud H II Region NGC 346 and the Primordial Helium Abundance

Spectrophotometry in the 3400-7400 range is presented for 13 areas of the brightest H II region in the SMC: NGC 346. The observations were obtained at CTIO with the 4-m telescope. Based on these observations its chemical composition is derived. The helium and oxygen abundances by mass are given by: Y(SMC)=0.2405+-0.0018 and O(SMC)=0.00171+-0.00025. From models and observations of irregular and blue compact galaxies it is found that dY/dO=3.5+-0.9 and consequently that the primordial helium abundance by mass is given by: Yp=0.2345+-0.0026 (1-sigma). This result is compared with values derived from Big Bang nucleosynthesis, and with other determinations of Yp.Comment: 32 pages + 5 figures Referee Revised Versio

Ascendiendo la escala del agua: servicios de abastecimiento de agua de usos multiples para la reduccion de la pobreza. [In Spanish]

Multiple use / Models / Water productivity / Research projects / Water supply / Domestic water / Irrigation water / Water governance / Poverty / Gender / Rural areas / Wells / Water harvesting / Runoff / Water storage / Water purification / Appropriate technology / Costs / Local government / Non governmental organizations / Case studies / Ethiopia / Nepal / Zimbabwe / Bolivia / India / Colombia / Thailand / South Africa