Severity of cardiovascular disease and health-related quality of life in men with prostate cancer: a longitudinal analysis from CaPSURE.

ObjectiveTo evaluate the influence of comorbid cardiovascular disease severity on health-related quality of life (HRQL) in men treated with radical prostatectomy (RP) or radiotherapy (RT) for early stage prostate cancer.MethodsSubjects (n=830) with non-metastatic disease who had been diagnosed in 2000-2002 were drawn from Cancer of the Prostate Strategic Urologic Research Endeavor (CaPSURE). We evaluated the influence of cardiovascular disease (CVD) severity on generic and disease-specific HRQL before and 6, 12, 18, and 24 months after treatment with RP or RT. HRQL was measured with the SF-36 and the UCLA Prostate Cancer Index.ResultsMen with moderate (n=193) or severe (n=51) cardiovascular disease had worse pre-treatment HRQL than did men without CVD (n=293) (P<0.01); HRQL scores were worse in men referred for RT. During 24 months of follow-up, men with moderate or severe CVD had worse SF-36 physical and mental component summaries and worse bowel function at all time points (P<0.05). Men with severe CVD also experienced a slower recovery in physical function (P=0.03) and sexual functioning (P=0.02) than did men without CVD.ConclusionsProstate cancer patients with moderate to severe CVD have worse HRQL during follow-up. Those with severe CVD recover their physical and sexual functioning more slowly after treatment

The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels

BACKGROUND: In seeds, the transition from dormancy to germination is regulated by abscisic acid (ABA) and gibberellins (GAs), and involves chromatin remodelling. Particularly, the repressive mark H3K27 trimethylation (H3K27me3) has been shown to target many master regulators of this transition. DAG1 (DOF AFFECTING GERMINATION1), is a negative regulator of seed germination in Arabidopsis, and directly represses the GA biosynthetic gene GA3ox1 (gibberellin 3-β-dioxygenase 1). We set to investigate the role of DAG1 in seed dormancy and maturation with respect to epigenetic and hormonal control. RESULTS: We show that DAG1 expression is controlled at the epigenetic level through the H3K27me3 mark during the seed-to-seedling transition, and that DAG1 directly represses also the ABA catabolic gene CYP707A2; consistently, the ABA level is lower while the GA level is higher in dag1 mutant seeds. Furthermore, both DAG1 expression and protein stability are controlled by GAs. CONCLUSIONS: Our results point to DAG1 as a key player in the control of the developmental switch between seed dormancy and germination

Satisfaction and annoyance of residents in neighbourhoods situated near industrial activity. Analysis of telephone survey data on the residential environment

In many of the Dutch national policy plans, environmental quality is a strong guiding principle. From an environmental science and policy perspective, environmental quality at the local level is affected by sound, odour and air pollution, as well as external safety risks. This study examines the relationship between people's perception of sound and odour (noise and odour annoyance) and the perceived environmental quality, represented as residential satisfaction (RS). Data from 19 telephone surveys (TLO) were compiled in a large TLO database. In the present study data from 17 TLOs performed in 86 locations, covering 18,000 respondents were analysed. Typically, TLOs are used for policy or maintenance purposes to assess the RS of people living in the vicinity of industrial plants or factories. Data on (individual) exposure levels were not available for these analyses. A remarkeble finding is the observed dispersion of correlations between annoyance and satisfaction per location (n=84, correlations for sound and satisfaction: -0,57 en 0,07; for odour and satisfaction between: -0,57 en 0,37). For this reason a multi-level analysis (MLA) was performed. This finding indicates location specific differences, probably due to differences in exposure levels. Furthermore, tenure and type of dwelling appeared to be relatively strong predictors of residential satisfaction. Homeowners appeared to be more satisfied than tenants. People in flats were more dissatisfied than people living in (semi-)detached houses. Annoyance from noise and (offensive) odours was another, relatively strong, predictor of RS. In general, residents reporting more annoyance were less satisfied with their residential situation. Other predictors of RS were age, gender, and the number of facilities in the dwelling. Older people and women were more satisfied with their residential situation than younger people and men, respectively. The more facilities present in the dwelling, the more satisfied residents appeared to be. Residential satisfaction is determined by a combination of subjective (e.g. annoyance), objective (e.g. number of facilities) and personal factors (e.g. age, gender). Annoyance appears to be a relatively important factor of residential satisfaction but tenure and dwelling type appear to be, relatively, more important. It is strongly recommended to add sound and odour exposure data to the TLO-database.Het begrip 'kwaliteit' speelt in steeds meer beleidsvoornemens van de (rijks)overheid een krachtig sturende rol. Voor de lokale omgeving, de leefomgeving, is de algemene beleidsdoelstelling van VROM het verbeteren en in stand houden van de woonkwaliteit. Belangrijke determinanten van de ervaren kwaliteit van de leefomgeving zijn (hinder van) geluid, geur, luchtverontreiniging en externe veiligheid. In dit onderzoek wordt de invloed van hinder van geluid en geur op de ervaren kwaliteit (tevredenheid) van de leefomgeving onderzocht. De belangrijkste vragen waren a) wat is de invloed van hinder op de woontevredenheid en b) welke persoons- en/of woonkenmerken zijn van invloed op de woontevredenheid? De gegevens zijn verzameld met behulp van Telefonische Leefsituatie Onderzoeken (TLO's) bij mensen die in de buurt van (industriele) bedrijvigheid wonen. Verschillende TLO's (n=19) zijn in een groot TLO-bestand samengevoegd. In deze analyse is gebruik gemaakt van 17 TLO's met daarin in totaal 86 onderzoekslocaties en ongeveer 18.000 respondenten. Vanwege de gelaagde structuur van de gegevens is uitgevoerd. Gegevens over (individuele) blootstellingniveaus waren voor deze analyses niet beschikbaar. Een opmerkelijk bevinding is de grote spreiding in de (cor)relatie tussen woontevredenheid en hinder per locatie (n=84, correlaties voor geluid tussen: -0,57 en 0,07; voor geur tussen -0,57 en 0,37). Dit is de reden waarom in afwijking van eerder onderzoek een multi-level analyse (MLA) is uitgevoerd. Deze bevinding duidt op locatie-specifieke verschillen die van invloed kunnen zijn op de relatie woontevredenheid en hinder. Verschillen in blootstellingsnivo's zouden hieraan ten grondslag kunnen liggen. De andere bevindingen zijn in overeenstemming met resultaten van soortgelijk onderzoek. De bezitsvorm van de woning (eigenaar-huurder) en het type woning blijken relatief belangrijke voorspellers van tevredenheid met de leefomgeving. In het algemeen zijn huurders minder tevreden dan eigenaren. Flatbewoners zijn minder tevreden dan mensen in (half)vrijstaande huizen. Daarnaast blijkt hinder (van lawaai en stank) een relatief belangrijke voorspeller van woontevredenheid te zijn. Naarmate respondenten meer hinder ondervinden zijn ze minder tevreden met hun leefomgeving. De leeftijd, het geslacht, en het aantal voorzieningen in de woning van de respondent zijn eveneens voorspellers van woontevredenheid, zij het in mindere mate. Vrouwen en oudere mensen zijn meer tevreden met hun leefomgeving dan mannen respectievelijk jongere mensen. Naarmate in een woning meer voorzieningen aanwezig zijn tonen de bewoners zich meer tevreden. De tevredenheid met de leefomgeving in woongebieden nabij industriele activiteit wordt bepaald door een mix van persoons-, belevings- en omgevingsgebonden kenmerken. De mate van hinder is een relatief belangrijke determinant van woontevredenheid maar is niet de belangrijkste. Bezitsvorm van de woning en type woning zijn, relatief gezien, belangrijker. Het is aan te bevelen de, mede op basis van de variatie in de relatie tussen hinder en woontevredenheid de TLO-data uit te breiden met (vergelijkbare) blootstellingsgegevens

Interleukin-10 negatively regulates local cytokine and chemokine production but does not influence antibacterial host defense during murine pneumococcal meningitis.

To determine the role of endogenous interleukin-10 (IL-10) in local host defense during pneumococcal meningitis, the inflammatory responses of IL-10-gene-deficient and wild-type mice after the induction of meningitis were compared. The absence of IL-10 was associated with higher cytokine and chemokine concentrations and a more pronounced infiltrate, but antibacterial defense or survival was not influenced

A Battery Certification Testbed for Small Satellite Missions

A battery pack consisting of standard cylindrical 18650 lithium-ion cells has been chosen for small satellite missions based on previous flight heritage and compliance with NASA battery safety requirements. However, for batteries that transit through the International Space Station (ISS), additional certification tests are required for individual cells as well as the battery packs. In this manuscript, we discuss the development of generalized testbeds for testing and certifying different types of batteries critical to small satellite missions. Test procedures developed and executed for this certification effort include: a detailed physical inspection before and after experiments; electrical cycling characterization at the cell and pack levels; battery-pack overcharge, over-discharge, external short testing; battery-pack vacuum leak and vibration testing. The overall goals of these certification procedures are to conform to requirements set forth by the agency and identify unique safety hazards. The testbeds, procedures, and experimental results are discussed for batteries chosen for small satellite missions to be launched from the ISS

Most Colorectal Cancer Survivors Live a Large Proportion of Their Remaining Life in Good Health

Purpose Colorectal cancer (CRC) diagnosis reduces life expectancy and decreases patients’ well-being. We sought to assess the determinants of health and functional status and estimate the proportion of remaining life that CRC survivors would spend in good health. Methods Using Sullivan method, healthy life expectancy was calculated based on survival data of 14,849 CRC survivors within a population-based cancer registry in southern Netherlands and quality of life information among a random sample of these survivors (n = 1,291). Results: Overall, albeit short life expectancy (LE at age 50 = 12 years for males and 13 years for females), most CRC survivors spent a large proportion of their remaining life in good health (74 and 77 %, for males and females, respectively). Long-term survivors may expect to live a normal life span (LE at age 50 = 30 years) and spent a large proportion of the remaining life in good health (78 %). In distinction, those with stage IV CRC had less than 2 years to live and spent more than half of their remaining life in poor health. Conclusions: Most CRC patients may expect no compromise on living a healthy life, underlining the importance of early detection. On the other hand, the high proportion of non-healthy years among stage IV CRC survivors confirms the importance of early detection and palliative care. Electronic supplementary material The online version of this article (doi:10.1007/s10552-012-0010-2) contains supplementary material, which is available to authorized users

Function and central projections of gustatory receptor neurons on the antenna of the noctuid moth Spodoptera littoralis

Chemosensory information is crucial for most insects to feed and reproduce. Olfactory signals are mainly used at a distance, whereas gustatory stimuli play an important role when insects directly contact chemical substrates. In noctuid moths, although the antennae are the main olfactory organ, they also bear taste sensilla. These taste sensilla detect sugars and hence are involved in appetitive learning but could also play an important role in food evaluation by detecting salts and bitter substances. To investigate this, we measured the responses of individual taste sensilla on the antennae of Spodoptera littoralis to sugars and salts using tip recordings. We also traced the projections of their neuronal axons into the brain. In each sensillum, we found one or two neurons responding to sugars: one NaCl-responsive and one water-sensitive neuron. Responses of these neurons were dose-dependent and similar across different locations on the antenna. Responses were dependent on the sex for sucrose and on both sex and location for glucose and fructose. We did not observe a spatial map for the projections from specific regions of the antennae to the deutocerebrum or the tritocerebrum/suboesophageal ganglion complex. In accordance with physiological recordings, back-fills from individual sensilla revealed up to four axons, in most cases targeting different projection zones

Coagulopathy in Zellweger spectrum disorders: a role for vitamin K

Introduction: Zellweger spectrum disorders (ZSDs) are caused by an impairment of peroxisome biogenesis, resulting in multiple metabolic abnormalities. This leads to a range of symptoms, including hepatic dysfunction and coagulopathy. This study evaluated the incidence and severity of coagulopathy and the effect of vitamin K supplementation orally and IV in ZSD. Methods: Data were retrospectively retrieved from the medical records of 30 ZSD patients to study coagulopathy and the effect of vitamin K orally on proteins induced by vitamin K absence (PIVKA-II) levels. Five patients from the cohort with a prolonged prothrombin time, low factor VII, and elevated PIVKA-II levels received 10 mg of vitamin K IV. Laboratory results, including thrombin generation, at baseline and 72 h after vitamin K administration were examined. Results: In the retrospective cohort, four patients (13.3%) experienced intracranial bleedings and 14 (46.7%) reported minor bleeding. No thrombotic events occurred. PIVKA-II levels decreased 38% after start of vitamin K therapy orally. In the five patients with a coagulopathy, despite treatment with oral administration of vitamin K, vitamin K IV caused an additional decrease (23%) of PIVKA-II levels and increased thrombin generation. Conclusion: Bleeding complications frequently occur in ZSD patients due to liver disease and vitamin K deficiency. Vitamin K deficiency is partly corrected by vitamin K supplementation orally, and vitamin K administered IV additionally improves vitamin K status, as shown by further decrease of PIVKA-II and improved thrombin generation

Metastable liquid-liquid phase transition in a single-component system with only one crystal phase and no density anomaly

We investigate the phase behavior of a single-component system in 3 dimensions with spherically-symmetric, pairwise-additive, soft-core interactions with an attractive well at a long distance, a repulsive soft-core shoulder at an intermediate distance, and a hard-core repulsion at a short distance, similar to potentials used to describe liquid systems such as colloids, protein solutions, or liquid metals. We showed [Nature {\bf 409}, 692 (2001)] that, even with no evidences of the density anomaly, the phase diagram has two first-order fluid-fluid phase transitions, one ending in a gas--low-density liquid (LDL) critical point, and the other in a gas--high-density liquid (HDL) critical point, with a LDL-HDL phase transition at low temperatures. Here we use integral equation calculations to explore the 3-parameter space of the soft-core potential and we perform molecular dynamics simulations in the interesting region of parameters. For the equilibrium phase diagram we analyze the structure of the crystal phase and find that, within the considered range of densities, the structure is independent of the density. Then, we analyze in detail the fluid metastable phases and, by explicit thermodynamic calculation in the supercooled phase, we show the absence of the density anomaly. We suggest that this absence is related to the presence of only one stable crystal structure.Comment: 15 pages, 21 figure