Liquidus Tracking: Controlled Rate Vitrification for the Cryopreservation of Larger Volumes and Tissues

BACKGROUND: Vitrification of cells or tissue at controlled cooling rates suitable for larger volumes, and with reduced cryoprotectant toxicity. OBJECTIVE: To set out the current understanding of the LiquidusTracking (LT) vitrification technique, and to discuss the challenges and benefits of translating the method into laboratory protocols more generally applicable to meet requirements of large volume and 3-D cryo-banking in the era of regenerative medicine. METHODS: By adding small amounts of cryoprotectants at each step and subsequently cooling the sample just above its freezing point before further increasing CPA concentration, cryoprotectant toxicity is minimized. RESULT: CPA toxicity can be reduced by lowering the temperature. Different manual approaches to LT were evaluated and further improved. CONCLUSIONS: Manual liquidus tracking is complicated and exhibits potential high variability. Nevertheless, this approach offers the possibility of testing several conditions simultaneously and could be used to pre-test conditions prior to automatic LT development

It’s all about information? The Following Behaviour of Professors and PhD Students on Twitter

In this paper we investigate the role of the academic status in the following behaviour of computer scientists on Twitter. Based on a uses and gratifications perspective, we focus on the activity of a Twitter account and the reciprocity of following relationships. We propose that the account activity addresses the users' information motive only, whereas the user's academic status relates to both the information motive and community development (as in peer networking or career planning). Variables were extracted from Twitter user data. We applied a biographical approach to correctly identify the academic status (professor versus PhD student). We calculated a $2\times 2$ MANOVA on the influence of the activity of the account and the academic status (on different groups of followers) to differentiate the influence of the information motive versus the motive for community development. Results suggest that for computer scientists Twitter is mainly an information network. However, we found significant effects in the sense of career planning, that is, the accounts of professors had even in the case of low activity a relatively high number of researcher followers -- both PhD followers as well as professor followers. Additionally, there was also some weak evidence for community development gratifications in the sense of peer-networking of professors. Overall, we conclude that the academic use of Twitter is not only about information, but also about career planning and networking

Small-scale swirl events in the quiet Sun chromosphere

Recent progress in instrumentation enables solar observations with high resolution simultaneously in the spatial, temporal, and spectral domains. We use such high-resolution observations to study small-scale structures and dynamics in the chromosphere of the quiet Sun. We analyze time series of spectral scans through the Ca II 854.2nm spectral line obtained with the CRISP instrument at the Swedish 1-m Solar Telescope. The targets are quiet Sun regions inside coronal holes close to disc-centre. The line core maps exhibit relatively few fibrils compared to what is normally observed in quiet Sun regions outside coronal holes. The time series show a chaotic and dynamic scene that includes spatially confined "swirl" events. These events feature dark and bright rotating patches, which can consist of arcs, spiral arms, rings or ring fragments. The width of the fragments typically appears to be on the order of only 0.2", which is close to the effective spatial resolution. They exhibit Doppler shifts of -2 to -4 km/s but sometimes up to -7 km/s, indicating fast upflows. The diameter of a swirl is usually of the order of 2". At the location of these swirls, the line wing and wide-band maps show close groups of photospheric bright points that move with respect to each other. A likely explanation is that the relative motion of the bright points twists the associated magnetic field in the chromosphere above. Plasma or propagating waves may then spiral upwards guided by the magnetic flux structure, thereby producing the observed intensity signature of Doppler-shifted ring fragments.Comment: 4 pages, 3 figures, A&A Letter, accepted (final version

3D photospheric velocity field of a Supergranular cell

We investigate the plasma flow properties inside a Supergranular (SG) cell, in particular its interaction with small scale magnetic field structures. The SG cell has been identified using the magnetic network (CaII wing brightness) as proxy, applying the Two-Level Structure Tracking (TST) to high spatial, spectral and temporal resolution observations obtained by IBIS. The full 3D velocity vector field for the SG has been reconstructed at two different photospheric heights. In order to strengthen our findings, we also computed the mean radial flow of the SG by means of cork tracing. We also studied the behaviour of the horizontal and Line of Sight plasma flow cospatial with cluster of bright CaII structures of magnetic origin to better understand the interaction between photospheric convection and small scale magnetic features. The SG cell we investigated seems to be organized with an almost radial flow from its centre to the border. The large scale divergence structure is probably created by a compact region of constant up-flow close to the cell centre. On the edge of the SG, isolated regions of strong convergent flow are nearby or cospatial with extended clusters of bright CaII wing features forming the knots of the magnetic network.Comment: 7 pages, submitted to A&A, referee's comments include

How Digital Are the Digital Humanities? An Analysis of Two Scholarly Blogging Platforms

In this paper we compare two academic networking platforms, HASTAC and Hypotheses, to show the distinct ways in which they serve specific communities in the Digital Humanities (DH) in different national and disciplinary contexts. After providing background information on both platforms, we apply co-word analysis and topic modeling to show thematic similarities and differences between the two sites, focusing particularly on how they frame DH as a new paradigm in humanities research. We encounter a much higher ratio of posts using humanities-related terms compared to their digital counterparts, suggesting a one-way dependency of digital humanities-related terms on the corresponding unprefixed labels. The results also show that the terms digital archive, digital literacy, and digital pedagogy are relatively independent from the respective unprefixed terms, and that digital publishing, digital libraries, and digital media show considerable cross-pollination between the specialization and the general noun. The topic modeling reproduces these findings and reveals further differences between the two platforms. Our findings also indicate local differences in how the emerging field of DH is conceptualized and show dynamic topical shifts inside these respective contexts

The GREGOR Fabry-P\'erot Interferometer

The GREGOR Fabry-P\'erot Interferometer (GFPI) is one of three first-light instruments of the German 1.5-meter GREGOR solar telescope at the Observatorio del Teide, Tenerife, Spain. The GFPI uses two tunable etalons in collimated mounting. Thanks to its large-format, high-cadence CCD detectors with sophisticated computer hard- and software it is capable of scanning spectral lines with a cadence that is sufficient to capture the dynamic evolution of the solar atmosphere. The field-of-view (FOV) of 50" x 38" is well suited for quiet Sun and sunspot observations. However, in the vector spectropolarimetric mode the FOV reduces to 25" x 38". The spectral coverage in the spectroscopic mode extends from 530-860 nm with a theoretical spectral resolution R of about 250,000, whereas in the vector spectropolarimetric mode the wavelength range is at present limited to 580-660 nm. The combination of fast narrow-band imaging and post-factum image restoration has the potential for discovery science concerning the dynamic Sun and its magnetic field at spatial scales down to about 50 km on the solar surface.Comment: 14 pages, 17 figures, 4 tables; pre-print of AN 333, p.880-893, 2012 (AN special issue to GREGOR

A retrospective of the GREGOR solar telescope in scientific literature

In this review, we look back upon the literature, which had the GREGOR solar telescope project as its subject including science cases, telescope subsystems, and post-focus instruments. The articles date back to the year 2000, when the initial concepts for a new solar telescope on Tenerife were first presented at scientific meetings. This comprehensive bibliography contains literature until the year 2012, i.e., the final stages of commissioning and science verification. Taking stock of the various publications in peer-reviewed journals and conference proceedings also provides the "historical" context for the reference articles in this special issue of Astronomische Nachrichten/Astronomical Notes.Comment: 6 pages, 2 color figures, this is the pre-peer reviewed version of Denker et al. 2012, Astron. Nachr. 333, 81

The Structure and Dynamics of the Upper Chromosphere and Lower Transition Region as Revealed by the Subarcsecond VAULT Observations

The Very high Angular resolution ULtraviolet Telescope (VAULT) is a sounding rocket payload built to study the crucial interface between the solar chromosphere and the corona by observing the strongest line in the solar spectrum, the Ly-a line at 1216 {\AA}. In two flights, VAULT succeeded in obtaining the first ever sub-arcsecond (0.5") images of this region with high sensitivity and cadence. Detailed analyses of those observations have contributed significantly to new ideas about the nature of the transition region. Here, we present a broad overview of the Ly-a atmosphere as revealed by the VAULT observations, and bring together past results and new analyses from the second VAULT flight to create a synthesis of our current knowledge of the high-resolution Ly-a Sun. We hope that this work will serve as a good reference for the design of upcoming Ly-a telescopes and observing plans.Comment: 28 pages, 11 figure

Discriminant analysis of solar bright points and faculae I. Classification method and center-to-limb distribution

While photospheric magnetic elements appear mainly as Bright Points (BPs) at the disk center and as faculae near the limb, high-resolution images reveal the coexistence of BPs and faculae over a range of heliocentric angles. This is not explained by a "hot wall" effect through vertical flux tubes, and suggests that the transition from BPs to faculae needs to be quantitatively investigated. To achieve this, we made the first recorded attempt to discriminate BPs and faculae, using a statistical classification approach based on Linear Discriminant Analysis(LDA). This paper gives a detailed description of our method, and shows its application on high-resolution images of active regions to retrieve a center-to-limb distribution of BPs and faculae. Bright "magnetic" features were detected at various disk positions by a segmentation algorithm using simultaneous G-band and continuum information. By using a selected sample of those features to represent BPs and faculae, suitable photometric parameters were identified in order to carry out LDA. We thus obtained a Center-to-Limb Variation (CLV) of the relative number of BPs and faculae, revealing the predominance of faculae at all disk positions except close to disk center (mu > 0.9). Although the present dataset suffers from limited statistics, our results are consistent with other observations of BPs and faculae at various disk positions. The retrieved CLV indicates that at high resolution, faculae are an essential constituent of active regions all across the solar disk. We speculate that the faculae near disk center as well as the BPs away from disk center are associated with inclined fields

Bright fibrils in Ca II K

Context: Except for the Ca II resonance lines, fibrils are ubiquitously present in most high-resolution observations of chromospheric lines. Aims: We show that fibrils are also a prevailing feature in Ca II K, provided the spatial-resolution is sufficiently high. Methods: We present high spatial resolution observations of an active region in the Ca I} K line from the Swedish Solar Telescope. Through a comparison between photospheric intensity and magnetic field data, we study the connection between bright chromospheric fibrils and photospheric structures. Additionally, using Fourier analysis we study how the fibrils are linked to the observed dynamics. Results: We find that very narrow, bright fibrils are a prevailing feature over large portions of the observed field. We also find a clear connection between the fibril footpoints and photospheric magnetic features. We show that the fibrils play two distinct roles in the observed dynamics: depending on their location they can act as a canopy suppressing oscillations or they can channel low-frequency oscillations into the chromosphere. Conclusions: The Ca II K fibrils share many characteristics with fibrils observed in other chromospheric lines, but some features, such as the very small widths, are unique to these observations.Comment: To be published in A&A. High resolution version can be downloaded from: http://www.mps.mpg.de/homes/pietarila/fibrils.pd