“Building from bottom” a success story

Rajkot Municipal Corporation (RMC) is a local government committed to provide basic infrastructure facilities including entertainment facilities to the people of the city. RMC is very well known for managing the city by using private sector participation as well as introduction of innovative mechanisms in management to serve people efficiently. City has prepared different plans for improving services and to nullify the gap between services and demands. The sole responsibility of Solid Waste Management (SWM) in the city lies with the Soild Wate Management department of Rajkot Muncipal Corporation (RMC)

Overexpression Analysis of emv2 gene coding for Late Embryogenesis Abundant Protein from Vigna radiata (Wilczek)

Late embryogenesis abundant (LEA) proteins are speculated to protect against water stress deficit in plants. An over expression system for mungbean late embryogenesis abundant protein, emv2 was constructed in a pET29a vector, designated pET-emv2 which is responsible for higher expression under the transcriptional/translational control of T7/lac promoter incorporated in the Escherichia coli BL21 (DE3).Induction protocol was optimized for pET recombinants harboring the target gene. Overexpressed EMV2 protein was purified to homogeneity and the protein profile monitored by SDS-PAGE

Fine mapping of rice drought QTL and study on combined effect of QTL for their physiological parameters under moisture stress condition

The present investigation was undertaken to study the effect of different yield QTL (DTY2.2, DTY3.1 and DTY8.1) under drought and their physiological response to drought stress. Backcross Inbred Lines (BILs) of IR64 (CB-193 and CB-229) along with IR64, APO and the traditional rice variety Norungan were raised in green house condition under water stress and control to evaluate the effect of the QTL on grain yield. The BIL CB-193 recorded higher photosynthetic rate (22.051), transpiration rate (7.152) and Ci/Ca ratio (0.597) whereas the BIL CB-229 recorded high relative water content (80.76%). It was found that the combination of three QTL (CB-229) performed better than the susceptible parent and the line with two QTL (CB-193 Fine-mapping of two QTLs viz., qDTY2.2 and qDTY8.1, for grain yield (GY) were conducted using backcross derived lines. Composite interval mapping analyses resolved the originally identified qDTY2.2 region of 6.7 cM into a segment of 2.1 cM and two sub QTLs at region between RM23132 and RM1578 (75.75 cM- 77.66 cM), RM515 and RM1578 (75.11 cM-77.66 cM) were identified in qDTY8.1 region. However this study provides a unique opportunity to breeders to introgress such regions together as a unit into high-yielding drought-susceptible varieties through MAS

Status of exploitation of tuna, mackerel and seerfish in Andaman and Nicobar Islands

Annual fish landing of Andaman and Nicobar Islands during 1989-'99 and the contribution of scombroids viz., tuna, mackerel and seerfishes are described. During the period the exploitation of tuna ranged between 321 to 3,8231, the average contribution being about 5% of the total fish landings. Among the tuna species, yellowfin tuna Thunnus albacares is the major contributor followed by skipjack tuna Katsuwonus pelamis and bigeye tuna Thunns obesus all of which are best caught during March to August. The mackerfel fishery is supported by Rastrelliger brachysoma followed by R.kanagurta. The total mackerel landings ranged between 806 to 1,9551, the major fishing season being from March to June and September to October and is best caught in the gill nets and boat seines. Seerfishes are dominantly represented by two genera and three species vjz. Scomberomorus commerson, S. guttatus and Acanthocybium solandri. Their major fishing season is from March to August and November to Febmary, being generally caught by gill nets, troll lines and hand lines. During the period of 1989 -'99, the total exploitation of seerfishes ranged between 293 to 1,172 t and showed a gradual increase in their production

Microbial decomposition of the floating weed Salvinia molesta Aublet in Cochin backwaters

From the live and decomposing S. molesta 13 strains of bacteria were isolated and the growth promoting effect of the weed extract in various concentrations in 5 selected strains of bacteria and a mixed culture was studied. Turbidity measurements gave maximum percentaae transmission in 5 % extract. Dry weight loss of dried weed in the laboratory was 20 and 25 % after to and 25 days of incubation respectively. Yield efficiency of 3 bacterial strains calculated for 48 hr was 20%. The results suggest the potential value of this primary source of organic material in the food web of Cocbin backwater

Unravelling the nature of HD 81032 - a new RS CVn Binary

BVR photometric and quasi-simultaneous optical spectroscopic observations of the star HD 81032 have been carried out during the years 2000 - 2004. A photometric period of $18.802 \pm 0.07$ d has been detected for this star. A large group of spots with a migration period of $7.43 \pm 0.07$ years is inferred from the first three years of the data. H$\alpha$ and Ca II H and K emissions from the star indicate high chromospheric activity. The available photometry in the BVRIJHK bands is consistent with spectral type of K0 IV previously found for this star. We have also examined the spectral energy distribution of HD 81032 for the presence of an infrared colour excess using the 2MASS JHK and IRAS photometry, but found no significant excess in any band abovethe normal values expected for a star with this spectral type. We have also analyzed the X-ray emission properties of this star using data obtained by the ROSAT X-ray observatory during its All-Sky Survey phase. An X-ray flare of about 12 hours duration was detected during the two days of X-ray coverage obtained for this star. Its X-ray spectrum, while only containing 345 counts, is inconsistent with a single-temperature component solar-abundance coronal plasma model, but implies either the presence of two or more plasma components, non-solar abundances, or a combination of both of these properties. All of the above properties of HD 81032 suggest that it is a newly identified, evolved RS CVn binary.Comment: 18 pages, 10 figures, 3 tables, Accepted for the publication in JAp

An environmentally benign antimicrobial nanoparticle based on a silver-infused lignin core

Silver nanoparticles have antibacterial properties, but their use has been a cause for concern because they persist in the environment. Here, we show that lignin nanoparticles infused with silver ions and coated with a cationic polyelectrolyte layer form a biodegradable and green alternative to silver nanoparticles. The polyelectrolyte layer promotes the adhesion of the particles to bacterial cell membranes and, together with silver ions, can kill a broad spectrum of bacteria, including Escherichia coli, Pseudomonas aeruginosa and quaternary-amine-resistant Ralstonia sp. Ion depletion studies have shown that the bioactivity of these nanoparticles is time-limited because of the desorption of silver ions. High-throughput bioactivity screening did not reveal increased toxicity of the particles when compared to an equivalent mass of metallic silver nanoparticles or silver nitrate solution. Our results demonstrate that the application of green chemistry principles may allow the synthesis of nanoparticles with biodegradable cores that have higher antimicrobial activity and smaller environmental impact than metallic silver nanoparticles

Spatial Variations of the Flood and Drought in the Northern Region of Sri Lanka

There are no any systematic orders in the occurrences of drought and flood hazards in Sri Lanka. Some areas have higher number of disaster occurrences and some other areas have lower numbers. In this situation this study focused the spatial variations of the drought and flood hazards in the Northern Region of Sri Lanka.  Main objective of this study is to identify the spatial patterns of drought and flood hazards in the study area. Monthly, Seasonal and annual climatic data for the stations Akkarayankulam, Ambalapperumalkulam, Iranaimadu, Kanukkerny, Karukkaikulam, Murunkan, Muththaiyankaddu, Nainathivu, Pallavarayankaddu, Pavatkulam, Thirunelveli, Vavunikkulam, and Vavuniya, were collected for the period from 1972 to 2012. Primary data for this study were collected using interviews and discussions and direct observations. Also varius sources of secondary data were used to carry out this study. Standardized Precipitation Index (SPI) has been used to identify the drought and flood hazards in every station for the period from 1972 to 2012. Krigging method of Arc GIS 9.2 version was used to identify the spatial variations of the drought and flood hazards.  During the South West Monsoon Season (SWMS) 03 severe drought in Vavunikkulam station and 09 in Akkarayankulam, 08 in Murungan, and 08 in Pavatkulam were identified. During the First Inter Monsoon Season (FIMS), extreme floods were experienced in several areas. Three in Iranaimadu, 03 in Thirunelveli, 03 in Pallavarayankaddu and 03 in Nainathivu station were identified in the Northern region using SPI method. During the Second Inter Monsoon Season (SIMS), 02 extreme floods in Thirunelveli, 02 in Vavuniya, and 03 in Murungan, and 02 in Akkarayankulam station and 07 severe droughts in Nainathivu and 05 in Vavuniya were also identified. During the North East Monsoon Season(NEMS), 03 extreme floods in Akkarayankulam, 03 in Ambalapperumalkulam and 03 severe floods, 03 extreme floods in Pallavaraykaddu, 03 severe floods in Muththaiyankaddu, 04 severe floods in Vavunikkulam station and 03 severe floods in Vavuniya were identified in the Northern region of Sri Lanka. Spatially some areas have been affected many times by flood and drought in the Northern Region of Sri Lanka. Comparatively, Eastern Parts of the study have more flood hazards and more number of droughts has occurred in the Western part of the study. Also 70% of the people expressed above thing. Also frequencies of the flood occurrences gradually decrease in the direction from the east to the west and similarly drought occurrences gradually decrease from the west to the east. Key Words: Drought, Flood, Seasons, Spatial Variations and Northern Regio