Protection of early phase hepatic ischemia-reperfusion injury by cholinergic agonists

BACKGROUND: Cytokine production is critical in ischemia/reperfusion (IR) injury. Acetylcholine binds to macrophages and inhibits cytokine synthesis, through the cholinergic anti-inflammatory pathway. This study examined the role of the cholinergic pathway in cytokine production and hepatic IR- injury. METHODS: Adult male mice underwent 90-min of partial liver ischemia followed by reperfusion. The AChR agonists (1,1-dimethyl-4-phenyl-L-pioperazinium-iodide [DMPP], and nicotine) or saline-vehicle were administered i.p. before ischemia. Plasma cytokine tumor necrosis factor (TNF)-α, macrophage inflammatory protein-2, and Interleukin-6 were measured. Liver injury was assessed by plasma alanine transaminase (ALT) and liver histopathology. RESULTS: A reperfusion time-dependent hepatocellular injury occurred as was indicated by increased plasma-ALT and histopathology. The injury was associated with marked elevation of plasma cytokines/chemokines. Pre-ischemic treatment of mice with DMPP or nicotine significantly decreased plasma-ALT and cytokines after 3 h of reperfusion. After 6 h of reperfusion, the protective effect of DMPP decreased and reached a negligible level by 24 h of reperfusion, despite significantly low levels of plasma cytokines. Histopathology showed markedly diminished hepatocellular injury in DMPP- and nicotine-pretreated mice during the early-phase of hepatic-IR, which reached a level comparable to saline-treated mice at late-phase of IR. CONCLUSION: Pharmacological modulation of the cholinergic pathway provides a means to modulate cytokine production and to delay IR-induced heaptocellular injury

White rot of sunflower

Goal. To study the spread and development of white rot on sunflower in the Right bank of Forest-Steppe of Ukraine. Methods. The research was conducted during 2014—2018 in the forest-steppe zone of Ukraine (Kyiv and Khmelnytsky region). The selection of plant samples was carried out with further analysis in laboratory conditions The observations were conducted according to generally accepted methods; spread and severity of diseases were determined. Results. During the years of investigation, the root, stem and head types of the disease have been identified. There were significant differences in both the symptoms and the degree of disease development in different years. During the vegetative periods of 2014, 2015, and 2017 disease development was compiled — 2—5%. Symptoms of the disease were detected on the stems of plants and heads, starting with the phase of seed development — milk ripeness. The weather conditions of 2016 and 2018 (GTK in June-July 1.2—1.9) were favorable for white rot infection. The disease appeared from the phase of “star” in 2016 and at the beginning of flowering in 2018. The development of the disease was significantly higher compared to other years and reached 12.0—15.1% in 2016 with the spread of 61.2—75.0%. In 2018 its severity in Khmelnitsky region was 11.2% while spread reached 78.6%. Firstly, the root and stem, and later the head form of the disease were marked. In addition to the typical symptoms, spots with brown concentric circles were found on the basal part of the stem. At the same time, the tissue almost did not collapse, and small sclerotic could be detected only in its lower part. Conclusions. Under favorable weather conditions, white rot of sunflower is becoming widespread and may be epiphytotic. In the conditions of the Right bank of Forest-Steppe Ukraine, the disease occurs at different stages of organogenesis. Symptoms of three forms of infection were observed. To improve the phytosanitary condition of crops, it is necessary to optimize the saturation of crop rotation with sunflower and other technical crops, which are also one of the host plants of the pathogen, and to follow of all elements of the protection system

White rot of sunflower

Goal. To study the spread and development of white rot on sunflower in the Right bank of Forest-Steppe of Ukraine.&#x0D; Methods. The research was conducted during 2014—2018 in the forest-steppe zone of Ukraine (Kyiv and Khmelnytsky region). The selection of plant samples was carried out with further analysis in laboratory conditions The observations were conducted according to generally accepted methods; spread and severity of diseases were determined.&#x0D; Results. During the years of investigation, the root, stem and head types of the disease have been identified. There were significant differences in both the symptoms and the degree of disease development in different years. During the vegetative periods of 2014, 2015, and 2017 disease development was compiled — 2—5%. Symptoms of the disease were detected on the stems of plants and heads, starting with the phase of seed development — milk ripeness. The weather conditions of 2016 and 2018 (GTK in June-July 1.2—1.9) were favorable for white rot infection. The disease appeared from the phase of “star” in 2016 and at the beginning of flowering in 2018. The development of the disease was significantly higher compared to other years and reached 12.0—15.1% in 2016 with the spread of 61.2—75.0%. In 2018 its severity in Khmelnitsky region was 11.2% while spread reached 78.6%. Firstly, the root and stem, and later the head form of the disease were marked. In addition to the typical symptoms, spots with brown concentric circles were found on the basal part of the stem. At the same time, the tissue almost did not collapse, and small sclerotic could be detected only in its lower part.&#x0D; Conclusions. Under favorable weather conditions, white rot of sunflower is becoming widespread and may be epiphytotic. In the conditions of the Right bank of Forest-Steppe Ukraine, the disease occurs at different stages of organogenesis. Symptoms of three forms of infection were observed. To improve the phytosanitary condition of crops, it is necessary to optimize the saturation of crop rotation with sunflower and other technical crops, which are also one of the host plants of the pathogen, and to follow of all elements of the protection system.</jats:p

Fungicidal protection of sunflower against the main leaf diseases

Goal. To determine the technical efficiency of the novel fungicides against the diseases of leaves sunflower and to establish the optimal terms of fungicidal treatments for the zone of the Right Bank Forest-steppe of Ukraine.&#x0D; Research methods. Field and laboratory. The research was conducted during 2014—2017 in the Right-bank Forest-steppe of Ukraine (Khmelnytsky region) on the Canyon hybrid investigated the effect of fungicides Pictor, SC (0.5 l / ha), Retengo, EC (0.75 l / ha), Amistar Extra 280 SC, SC (0.75 l / ha) was investigated. The treatments were carried out in two terms: once — on 32 and 53 stages on the BBCH scale and twice — at the same terms. The records were performed before application and on the 15th and 30th day after it. The observations were conducted according to generally accepted methods; the incidence and severity of diseases, technical efficiency were determined.&#x0D; Results. It was determined that crops annually struck affected leaf disease Alternaria and Septoria. The highest development was recorded for alternaria leaf spot — 22.5%. For both single and double spraying, the highest level of protection against septoria leaf blotch was observed for the application of Amistar Extra 280 SC, SC (0.75 l / ha), while Pictor, SC (0.5 l / ha) was more efficient against alternaria leaf spot.&#x0D; Conclusions. Te two-fold application of fungicides is more effective, it reduces the development of leaf diseases by 78—96% and preserves the crop within 0,41—0,53 t / ha. Among the studied fungicides, Pictor, EC and Amistar Extra 280 SC, SC showed superior efficacy for all investigated terms.</jats:p

Slime mold (Physarum cinereum (Batsch) Pers.) on turfgrass

Goal. To study the spread and severity of slime mold on turfgass in Ukraine. Methods. Field and laboratory methods were used. The research was conducted on two varieties of mixed with different percentage and species composition of cereal grasses. The survey of crops was performed during vegetation in the Polissya, Forest-steppe and Steppe areas. For the identification of pathogen, a selection of plant samples was carried out with further analysis in laboratory conditions. The observations were conducted according to generally accepted methods; spread and severity of disease were determined. Results. The first time in Ukraine revealed damage to the cereal grasses (Physarum cinereum (Batsch) Pers.). Symptoms and morphological characteristics of the pathogen are described. In the Kiev region, in terms of distribution and development, it exceeded other pathogens (30.6—39.0% and 15.4—21.2% correspondingly). In the Kirovograd region, slime mold was observed in 2016 and 2017 at a much lower degree. Its spread was 8.4—10.5% with severity within the range of 3.1—4.5%. In the Zhytomyr region during the period of research symptoms of this disease were not fixed. The most favorable for the development of Physarum cinereum was the growing season of 2017. Conclusions. For the first time in Ukraine, the appearance of disease caused by Physarum cinereum was detected on lawn grasses. The spread of this disease has been noted on both types of grass mixtures. The phytopathological analysis showed that slime mold was the most widespread diseases in the Forest-Steppe zone (Kyiv region), where it dominated in the complex of leaf diseases. In Steppe zone (Kirovograd region) in the period of 2016—2017 it's spread was much lower and in Polissya zone (Zhytomyr region) symptoms of slime mold was not revealed

Slime mold (Physarum cinereum (Batsch) Pers.) on turfgrass

Goal. To study the spread and severity of slime mold on turfgass in Ukraine.&#x0D; Methods. Field and laboratory methods were used. The research was conducted on two varieties of mixed with different percentage and species composition of cereal grasses. The survey of crops was performed during vegetation in the Polissya, Forest-steppe and Steppe areas. For the identification of pathogen, a selection of plant samples was carried out with further analysis in laboratory conditions. The observations were conducted according to generally accepted methods; spread and severity of disease were determined.&#x0D; Results. The first time in Ukraine revealed damage to the cereal grasses (Physarum cinereum (Batsch) Pers.). Symptoms and morphological characteristics of the pathogen are described. In the Kiev region, in terms of distribution and development, it exceeded other pathogens (30.6—39.0% and 15.4—21.2% correspondingly). In the Kirovograd region, slime mold was observed in 2016 and 2017 at a much lower degree. Its spread was 8.4—10.5% with severity within the range of 3.1—4.5%. In the Zhytomyr region during the period of research symptoms of this disease were not fixed. The most favorable for the development of Physarum cinereum was the growing season of 2017.&#x0D; Conclusions. For the first time in Ukraine, the appearance of disease caused by Physarum cinereum was detected on lawn grasses. The spread of this disease has been noted on both types of grass mixtures. The phytopathological analysis showed that slime mold was the most widespread diseases in the Forest-Steppe zone (Kyiv region), where it dominated in the complex of leaf diseases. In Steppe zone (Kirovograd region) in the period of 2016—2017 it's spread was much lower and in Polissya zone (Zhytomyr region) symptoms of slime mold was not revealed.</jats:p

Fungicidal protection of sunflower against the main leaf diseases

Goal. To determine the technical efficiency of the novel fungicides against the diseases of leaves sunflower and to establish the optimal terms of fungicidal treatments for the zone of the Right Bank Forest-steppe of Ukraine. Research methods. Field and laboratory. The research was conducted during 2014—2017 in the Right-bank Forest-steppe of Ukraine (Khmelnytsky region) on the Canyon hybrid investigated the effect of fungicides Pictor, SC (0.5 l / ha), Retengo, EC (0.75 l / ha), Amistar Extra 280 SC, SC (0.75 l / ha) was investigated. The treatments were carried out in two terms: once — on 32 and 53 stages on the BBCH scale and twice — at the same terms. The records were performed before application and on the 15th and 30th day after it. The observations were conducted according to generally accepted methods; the incidence and severity of diseases, technical efficiency were determined. Results. It was determined that crops annually struck affected leaf disease Alternaria and Septoria. The highest development was recorded for alternaria leaf spot — 22.5%. For both single and double spraying, the highest level of protection against septoria leaf blotch was observed for the application of Amistar Extra 280 SC, SC (0.75 l / ha), while Pictor, SC (0.5 l / ha) was more efficient against alternaria leaf spot. Conclusions. Te two-fold application of fungicides is more effective, it reduces the development of leaf diseases by 78—96% and preserves the crop within 0,41—0,53 t / ha. Among the studied fungicides, Pictor, EC and Amistar Extra 280 SC, SC showed superior efficacy for all investigated terms

BIOLOGICAL AGENTS TO PROTECT OATS AGAINST DISEASES IN RIGHTBANK FOREST STEPPE UKRAINE

Species composition of oats diseases in the Right-bank Forest-steppe of Ukraine has been clarified. Results of application of biological fungicides by the method of seed dressing and surface treatment for the protection of tunicate (Chernihivskyi 28 variety) and hull-less (Samuel variety) oats are provided.</jats:p

Fungicides against diseases of lawn grass leaves

Goal. To evaluate the effectiveness of the use of fungicides of various chemical groups against the leaf diseases turfgrass.&#x0D; Methods. The research was carried out in 2015—2017 on the lawn mixture «Universal» in the zone of the Polissya of Ukraine. The effect of fungicides of various chemical groups was investigated (Impact T, SC; Bumper Super, EC; Amistar Extra 280 SC, SC; Alto Super 300 EC, EC; Maxim 025 FS, FS; Horus 75 WG, WG). Fungicides were applied twice. The first spraying was carried out when development of diseases in control plot reached 3—5%, the second — in 30 days. The assessments were performed according to generally accepted methods, disease spread, disease severity and technical efficiency of pesticides were determined.&#x0D; Results. During the period of research, the most common leaf diseases of turfgrass, which eveloped annually and had a significant severity during the growing season, were powdery mildew and septoria leaf blotch. After two-time spraying, the technical efficiency of fungicides against powdery mildew reached 82.2—92.2%, against septoria leaf blotch up to 80.2%. The most effective against powdery mildew was the use of Alto Super 330 EC, EC (0.5 l / ha), Amistar Extra 280 SC, SC (0.75 l / ha) and tank mixture of Maxim 025 FS, FS (0.75 l / ha and 1.0 l / ha) with Horus 75 WG, VH (0.6 kg / ha). Against the septoria leaf blotch, the highest efficiency was shown by Alto Super 330 EC, EC (0.5 l / ha), Bumper Super, EC (0.8 l / ha) and Maxim 025 FS, FS (1.0 l / ha) + Horus 75 WG, WG (0.6 kg / ha). In addition, due to the reduction of disease severity, a positive effect of fungicides on the quality of the lawn was achieved and the projective coating increased to 92—93%.&#x0D; Conclusion. The use of fungicides contributes to the reduction of disease and the formation of quality herbs. The best indicators against flour dew and septoriosis of leaves were obtained for the treatment of Alto Super 330 EC, CE (0.5 l / ha) and tank Maxim 025 FS, tc.s. (1.0 l / ha) + Horus 75 WG, VH (0.6 kg / ha).</jats:p

Fungicides against diseases of lawn grass leaves

Goal. To evaluate the effectiveness of the use of fungicides of various chemical groups against the leaf diseases turfgrass. Methods. The research was carried out in 2015—2017 on the lawn mixture «Universal» in the zone of the Polissya of Ukraine. The effect of fungicides of various chemical groups was investigated (Impact T, SC; Bumper Super, EC; Amistar Extra 280 SC, SC; Alto Super 300 EC, EC; Maxim 025 FS, FS; Horus 75 WG, WG). Fungicides were applied twice. The first spraying was carried out when development of diseases in control plot reached 3—5%, the second — in 30 days. The assessments were performed according to generally accepted methods, disease spread, disease severity and technical efficiency of pesticides were determined. Results. During the period of research, the most common leaf diseases of turfgrass, which eveloped annually and had a significant severity during the growing season, were powdery mildew and septoria leaf blotch. After two-time spraying, the technical efficiency of fungicides against powdery mildew reached 82.2—92.2%, against septoria leaf blotch up to 80.2%. The most effective against powdery mildew was the use of Alto Super 330 EC, EC (0.5 l / ha), Amistar Extra 280 SC, SC (0.75 l / ha) and tank mixture of Maxim 025 FS, FS (0.75 l / ha and 1.0 l / ha) with Horus 75 WG, VH (0.6 kg / ha). Against the septoria leaf blotch, the highest efficiency was shown by Alto Super 330 EC, EC (0.5 l / ha), Bumper Super, EC (0.8 l / ha) and Maxim 025 FS, FS (1.0 l / ha) + Horus 75 WG, WG (0.6 kg / ha). In addition, due to the reduction of disease severity, a positive effect of fungicides on the quality of the lawn was achieved and the projective coating increased to 92—93%. Conclusion. The use of fungicides contributes to the reduction of disease and the formation of quality herbs. The best indicators against flour dew and septoriosis of leaves were obtained for the treatment of Alto Super 330 EC, CE (0.5 l / ha) and tank Maxim 025 FS, tc.s. (1.0 l / ha) + Horus 75 WG, VH (0.6 kg / ha)