Understanding the Effects of a Tannin Extract on Forage Protein Digestion in the Rumen and Abomasum Using a Dynamic Artificial Digestive System Coupled to a Digestomic Approach

Improving the use efficiency of dietary protein in ruminants is a major challenge to decrease feed supplementation and significantly decrease nitrogen (N) losses to the environment. The aim of this study was to characterize the effects of tannins on protein digestion in the rumen and in conditions simulating the abomasum, using a dynamic in vitro digestive system coupled to a digestomic approach. Three ruminally-cannulated sheep fed with alfalfa hay were infused daily with a solution of tannins, while three other sheep were infused with water (control). Standardized ruminal fluid was introduced into the digester, which simulated the transit of digesta under physicochemical conditions mimicking the abomasum in terms of pH regulation, digestive enzyme infusions and transit rate. Protein degradation in the rumen and in the simulated abomasum was analyzed by determination of fermentation end-products, and identification and quantification of peptides (Label Free Quantification) by LC-MS/MS high resolution (Orbitrap). The analysis of rumen samples showed that tannins result in a clear decrease of fermentation end-products related to protein degradation, namely ammonia (NH3) and iso-volatile fatty acids (VFA), and a greater abundance of the Rubisco, a major plant protein. In the simulated abomasal compartment, the peptidomic analysis showed that the hydrolysis intensity of Rubisco was higher in the presence of tannins compared to the control group. These results indicate that protein-tannin complexes could be dissociated in the physico-chemical conditions of the abomasum, increasing the flow of peptides to the intestine after protection of protein by tannins in the rumen

Immunohistochemical expression of latent membrane protein 1 (LMP1) and p53 in nasopharyngeal carcinoma: Moroccan experience

BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor intimately associated with Epstein-Barr virus (EBV). NPC is a characteristic tumor displaying epidemiological, genetic and regional distribution properties that makes it unique by its natural behavior. OBJECTIVES: To assess the expression pattern of LMP1 and p53 proteins in the different histological types of NPC in a sample of the Moroccan population and to define any association between the expression of those proteins with the sex, the age and the histological types of NPC. METHODS: Archival formalin-fixed, paraffin-embedded NPC biopsies were evaluated in 23 Moroccan patients for the presence of LMP1 and p53 using immunohistochemistry (IHC). RESULTS: No LMP1 expression was observed whereas 8 of 23 cases (34. 7%) had detectable p53 protein in the nuclei of tumor cells. After statistical analysis according to the Fisher's exact probability test, no significant association between p53 expression and histological type, age and sex distributions was demonstrated (p>0.05). CONCLUSION: This study confirms that p53 overexpression is present in a subset of Moroccan NPC patients. Our results are consistent with those reported by other studies concerning the same NPC endemic risk area and provide original data concerning Morocco

Prevención de adicciones desde la construcción de redes sociales comunitarias. Bajo el marco de la Ley Nacional 26.061 de Protección Integral de los Derechos de las Niñas, Niños y Adolescentes.

La presente investigación se centra en la prevención de adicciones en adolescentes, tomando como muestra aproximada a 30 alumnos/as de 13 a 18 años en dos escuelas públicas secundarias: “Dr. Eduardo J. Chahla (Guaymallén) y “Profesores Mendocinos” (Luján de Cuyo) ambas ubicadas en zonas urbano marginales