Studi Kualitas Perairan Pantai di Kawasan Industri Perikanan, Desa Pengambengan, Kecamatan Negara, Kabupaten Jembrana

Bali Strait represent the strait dissociating Bali Island with Java Island. As an area of fishing zone, Bali Straitowns a significant fishery potency, especially lemuru fish (Sardenella lemuru). For the facility of fisherman haul,governmental developed the Center of Fish Landing which is located at Pengambengan village of Negara Subdistrict.Within the area also exists the fishery industries which is operated for fish canning and fish meal.The aim of this research was to determine the properties of pollutant from waste water that was produced byfishery industry, as well as to know the quality of coastal waters destined for the aquatic life that produced by industrialactivity.The sample of sea waters was taken from 11 locations and one sample was taken from waste water effluent one ofone factory at Pengambengan village. The result of analysis was used to calculate the pollution index and comparationwas made to the Governor Regulation Number 8 year 2007.The finding of the study shows that the pollutant properties of the waste water produced by fishery industry haveexceeded the maximum level of quality standar covering parameters of TSS, TDS, BOD5, COD, sulfide hydrogen (H2S),degree of acidity (pH), and ammonia (NH3). Calculation result of respective sampling location, shows that the value ofthe pollution index varied from slight, medium, and heavy pollution levels

Studi Kualitas Perairan Pantai di Kawasan Industri Perikanan, Desa Pengambengan, Kecamatan Negara, Kabupaten Jembrana

Bali Strait represent the strait dissociating Bali Island with Java Island. As an area of fishing zone, Bali Straitowns a significant fishery potency, especially lemuru fish (Sardenella lemuru). For the facility of fisherman haul,governmental developed the Center of Fish Landing which is located at Pengambengan village of Negara Subdistrict.Within the area also exists the fishery industries which is operated for fish canning and fish meal.The aim of this research was to determine the properties of pollutant from waste water that was produced byfishery industry, as well as to know the quality of coastal waters destined for the aquatic life that produced by industrialactivity.The sample of sea waters was taken from 11 locations and one sample was taken from waste water effluent one ofone factory at Pengambengan village. The result of analysis was used to calculate the pollution index and comparationwas made to the Governor Regulation Number 8 year 2007.The finding of the study shows that the pollutant properties of the waste water produced by fishery industry haveexceeded the maximum level of quality standar covering parameters of TSS, TDS, BOD5, COD, sulfide hydrogen (H2S),degree of acidity (pH), and ammonia (NH3). Calculation result of respective sampling location, shows that the value ofthe pollution index varied from slight, medium, and heavy pollution levels

Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques

NoGrowing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 μm and 141 ± 70 μm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications.(Science Fund Vot No.: 0201-01-13-SF0104 or S024) awarded by Malaysia Ministry of Science and Technology (MOSTI) and IGSP Grant Vot No. U679 awarded by Universiti Tun Hussein Onn Malaysia