Five-Brane Effective Field Theory on Calabi-Yau Threefolds

We consider the compactification of the dual form of $N=1$ $D=10$ supergravity on a six-dimensional Calabi-Yau manifold. An $N=1$ off-shell supergravity effective Lagrangian in four dimensions can be constructed in a dual version of the gravitational sector (new-minimal supergravity form). Superspace duality has a simple interpretation in terms of Poincar\'{e} duality of two-form cohomology. The resulting $4D$ Lagrangian may describe the low-energy point-field limit of a five-brane theory, dual to string theory, provided Calabi-Yau spaces are consistent vacua of such dual theory.Comment: 16 pages, LaTeX, CERN-Th.6914/93 POLFIS-TH.04/93 UCLA/93/TEP/1

Structure of the Chiral Scalar Superfield in Ten Dimensions

We describe the tensors and spinor-tensors included in the $\theta$-expansion of the ten-dimensional chiral scalar superfield. The product decompositions of all the irreducible structures with $\theta$ and the $\theta^2$ tensor are provided as a first step towards the obtention of a full tensor calculus for the superfield.Comment: 50 pages, UCLA/94/TEP/

Whales vs. gulls: Assessing trade-offs in wildlife and waste management in Patagonia, Argentina

© 2015 Elsevier B.V.In Península Valdés, (Patagonia) Argentina, the consequences of poor waste management and an overpopulation of kelp gulls has led to gulls feeding on living southern right whales, potentially causing losses to the tourism industry through loss in coastal quality and suboptimal right whale viewing experiences. Despite local progress in closing waste disposal sites and culling gulls, both waste and pest problems persist. While this problem could impact the long-term viability of the site as a whale watching destination and present conservation concerns, little research has been done concerning the socio-economic aspects of the problem. The present study interviewed 650 tourists about their willingness to pay to manage the gulls versus the waste in order to reduce the gull population and remove the risk to the whales. This research finds that tourists favor addressing the human-driven component of the problem, the waste, over culling the natural component of the problem, the kelp gulls. These findings present a remarkable insight to assessing trade-offs between two management strategies to a local problem associated with coastal development and tourism. The results could further be broadened to other destinations facing waste and pest management challenges in the face of growing tourism and urbanization

Mitigating slipping-related mortality from purse seine fisheries for small pelagic fish: case studies from European Atlantic waters

The release of unwanted catches (UWC) from purse seines, while the catch is still in the water, is known as “slipping”. Once thought to be a benign process, compared to discarding UWC overboard from the fishing vessel, it is now recognised that “slipping” can lead to significant mortality in the released fish if done inappropriately. In this chapter, we examine purse seining and slipping operations, and discuss what drives slipping and potential mitigation measures to reduce slipping mortality. We use three examples of purse seine fisheries for small pelagic species in the North-east Atlantic; from Norway, Portugal and Spain. The ideal solution (identifying and avoiding UWC before the net is set) requires the development of tools to enable fishers to better characterise target schools in terms of key selection criteria, e.g., with respect to species, individual size and catch biomass. Such tools are being developed, based primarily on hydro-acoustic technology. However, some UWC in purse seine catches are inevitable, and operational improvements in slipping practices have been shown to significantly reduce stress and mortality in the released UWC. We conclude with a discussion on the challenges currently facing the implementation of the European Union (EU) Landing Obligation with regards to minimising slipping related mortality.info:eu-repo/semantics/publishedVersio

The structure of an endogenous Drosophila centromere reveals the prevalence of tandemly repeated sequences able to form i-motifs

Centromeres are the chromosomal loci at which spindle microtubules attach to mediate chromosome segregation during mitosis and meiosis. In most eukaryotes, centromeres are made up of highly repetitive DNA sequences (satellite DNA) interspersed with middle repetitive DNA sequences (transposable elements). Despite the efforts to establish complete genomic sequences of eukaryotic organisms, the so-called 'finished' genomes are not actually complete because the centromeres have not been assembled due to the intrinsic difficulties in constructing both physical maps and complete sequence assemblies of long stretches of tandemly repetitive DNA. Here we show the first molecular structure of an endogenous Drosophila centromere and the ability of the C-rich dodeca satellite strand to form dimeric i-motifs. The finding of i-motif structures in simple and complex centromeric satellite DNAs leads us to suggest that these centromeric sequences may have been selected not by their primary sequence but by their ability to form noncanonical secondary structures.Peer Reviewe

Real Special Geometry

We give a coordinate-free description of real manifolds occurring in certain four dimensional supergravity theories with antisymmetric tensor fields. The relevance of the linear multiplets in the compactification of string and five-brane theories is also discussed.Comment: 10 pgs (TeX with Harvmac), CERN-TH.7211/94, UCLA/94/TEP/14, POLFIS-TH.01/9

Duality Transformations in Supersymmetric Yang-Mills Theories coupled to Supergravity,

We consider duality transformations in N=2, d=4 Yang--Mills theory coupled to N=2 supergravity. A symplectic and coordinate covariant framework is established, which allows one to discuss stringy `classical and quantum duality symmetries' (monodromies), incorporating T and S dualities. In particular, we shall be able to study theories (like N=2 heterotic strings) which are formulated in symplectic basis where a `holomorphic prepotential' F does not exist, and yet give general expressions for all relevant physical quantities. Duality transformations and symmetries for the N=1 matter coupled Yang--Mills supergravity system are also exhibited. The implications of duality symmetry on all N>2 extended supergravities are briefly mentioned. We finally give the general form of the central charge and the N=2 semiclassical spectrum of the dyonic BPS saturated states (as it comes by truncation of the N=4 spectrum).Comment: 41 pages, harvmac, no figures. References added, and minor printing error corrected

Tubulin isoform composition tunes microtubule dynamics

Microtubules polymerize and depolymerize stochastically, a behavior essential for cell division, motility and differentiation. While many studies advanced our understanding of how microtubule-associated proteins tune microtubule dynamics in trans, we have yet to understand how tubulin genetic diversity regulates microtubule functions. The majority of in vitro dynamics studies are performed with tubulin purified from brain tissue. This preparation is not representative of tubulin found in many cell types. Here we report the 4.2Å cryo-EM structure and in vitro dynamics parameters of α1B/βI+βIVb microtubules assembled from tubulin purified from a human embryonic kidney cell line with isoform composition characteristic of fibroblasts and many immortalized cell lines. We find that these microtubules grow faster and transition to depolymerization less frequently compared to brain microtubules. Cryo-EM reveals that the dynamic ends of α1B/βI+βIVb microtubules are less tapered and that these tubulin heterodimers display lower curvatures. Interestingly, analysis of EB1 distributions at dynamic ends suggests no differences in GTP cap sizes. Lastly, we show that the addition of recombinant α1A/βIII tubulin, a neuronal isotype overexpressed in many tumors, proportionally tunes the dynamics of α1B/βI+βIVb microtubules. Our study is an important step towards understanding how tubulin isoform composition tunes microtubule dynamics

Tubulin Binds to the Cytoplasmic Loop of TRESK Background K+ Channel In Vitro.

The cytoplasmic loop between the second and third transmembrane segments is pivotal in the regulation of TRESK (TWIK-related spinal cord K+ channel, K2P18.1, KCNK18). Calcineurin binds to this region and activates the channel by dephosphorylation in response to the calcium signal. Phosphorylation-dependent anchorage of 14-3-3 adaptor protein also modulates TRESK at this location. In the present study, we identified molecular interacting partners of the intracellular loop. By an affinity chromatography approach using the cytoplasmic loop as bait, we have verified the specific association of calcineurin and 14-3-3 to the channel. In addition to these known interacting proteins, we observed substantial binding of tubulin to the intracellular loop. Successive truncation of the polypeptide and pull-down experiments from mouse brain cytosol narrowed down the region sufficient for the binding of tubulin to a 16 amino acid sequence: LVLGRLSYSIISNLDE. The first six residues of this sequence are similar to the previously reported tubulin-binding region of P2X2 purinergic receptor. The tubulin-binding site of TRESK is located close to the protein kinase A (PKA)-dependent 14-3-3-docking motif of the channel. We provide experimental evidence suggesting that 14-3-3 competes with tubulin for the binding to the cytoplasmic loop of TRESK. It is intriguing that the 16 amino acid tubulin-binding sequence includes the serines, which were previously shown to be phosphorylated by microtubule-affinity regulating kinases (MARK kinases) and contribute to channel inhibition. Although tubulin binds to TRESK in vitro, it remains to be established whether the two proteins also interact in the living cell