Discovery and characterisation of an amidine-containing ribosomally-synthesised peptide that is widely distributed in nature

ABSTRACTRibosomally synthesised and post-translationally modified peptides (RiPPs) are a structurally diverse class of natural product with a range of bioactivities. Genome mining for RiPP biosynthetic gene clusters (BGCs) is often hampered by poor detection of the short precursor peptides that are ultimately modified into the final molecule. Here, we utilise a previously described genome mining tool, RiPPER, to identify novel RiPP precursor peptides near YcaO-domain proteins, enzymes that catalyse various RiPP post-translational modifications including heterocyclisation and thioamidation. Using this dataset, we identified a novel, diverse and highly conserved family of RiPP BGCs spanning over 230 species of Actinobacteria and Firmicutes. A representative BGC from Streptomyces albus J1074 was characterised, leading to the discovery of streptamidine, a novel-amidine containing RiPP. This highlights the breadth of unexplored natural products with structurally rare features, even in model organisms.</jats:p

Discovery and characterisation of an amidine-containing ribosomally-synthesised peptide that is widely distributed in nature

Genome mining for pathways containing YcaO proteins revealed a widespread novel family of RiPP gene clusters. A model gene cluster was characterised through genetic and chemical analyses, which yielded streptamidine, a novel amidine-containing RiPP.</jats:p

Trabajo y salud de las docentes de la Universidad Nacional de Córdoba: uso de medicamentos/sustancias lícitas y plan materno infantil Trabalho e saúde das docentes da Universidade Nacional de Córdoba: uso de medicamentos/substâncias lícitas e plano materno infantil Work and health of the Cordoba National University teachers: medicaments/legal drugs use and infantile-maternal plan

Este trabajo tuvo como objetivo investigar condiciones de existencia, proceso de trabajo y salud y consumo de medicamentos de docentes universitarias en edad reproductiva. Además, intentó conocer uso, opiniones y sugerencias de usuarias del Plan Materno Infantil de la Obra Social Universitaria. El estudio se encaminó desde la perspectiva teórico-metodológica del materialismo histórico y dialéctico, se delineó como investigación social desarrollada a través de un estudio de campo descriptivo-correlativo e estratégico en una muestra total de 50 personas. Los resultados indican presencia de condiciones de existencia y trabajo deterioradas, afectando el perfil de salud de las docentes y el consumo de medicamentos. Alto porcentaje de las entrevistadas consideró el Plan Materno Infantil muy bueno o bueno y enfatizó la necesidad de mejor información a cerca de ella.<br>Este trabalho teve como objetivo investigar condições de existência, processo de trabalho e saúde e consumo de medicamentos de docentes universitárias em idade reprodutiva. Além disso, buscou conhecer uso, opiniões e sugestões de usuárias do Plano Materno Infantil da Obra Social Universitária. O estudo encaminhou-se pela perspectiva teórico-metodológica do materialismo histórico e dialético, delineou-se como investigação social desenvolvida por um estudo de campo descritivo-correlativo e estratégico, em uma mostra total de 50 pessoas. Os resultados indicam presença de condições de existência e trabalho deterioradas, afetando o perfil de saúde das docentes e o consumo de medicamentos. Alta porcentagem das entrevistadas considerou o Plano Materno Infantil bom ou muito bom e enfatizou a necessidade de melhor informação sobre ele.<br>This study aimed to investigate university teachers in reproductive age' life conditions, work and health process and medicaments use. Beside, aimed research the use, opinions and suggestions of Infantile-Maternal Plan of the University Social Assistance clients. In dialectic and historic materialism theory and method way, this is a social research developed by a strategic and correlative-descriptive fieldwork with 50 persons sample. The results showed degraded work and life conditions, affecting the teachers' health profile and medicaments use. The Infantile-Maternal Plan is good or very good and demands best information accord the teachers in high percent opinion

Kernohan-Woltman notch phenomenon: an exceptional neurological picture?

20.500.12530/87908Ipsilateral hemiparesis (IH) can be defined as a paradoxical dysfunction of the first motor neuron involving the extremities on the opposite side to that expected, given the location of the triggering intracranial pathology. Compression of the corticospinal tract (CSt) along its course through the contralateral cerebral peduncle against the free edge of the tentorium, known as the Kernohan-Woltman notch phenomenon (KWNP), represents the main cause of IH. This retrospective study analyses a series of 12 patients diagnosed with IH secondary to KWNP treated at our institution, including a descriptive study of epidemiological, clinical, radiological, neurophysiological, and prognostic variables. In 75% of the cases, symptoms had an acute or subacute onset. Initial imaging studies showed signs of significant mass effect in half of the patients, whereas magnetic resonance imaging (MRI) identified a structural lesion in the contralateral cerebral peduncle in two thirds of them. Impairment of the motor evoked potentials (MEP) was verified in 4 patients. During follow-up 7 patients experienced improvement in motor activity, and near half of the cases were classified in the first three categories of the modified Rankin scale. In contrast to prior historical series, most of our patients developed a KWNP secondary to a traumatic mechanism. MRI represents the optimal method to identify both the classic cerebral peduncle notch and the underlying structural lesion of the CSt. The use of MEP can help to establish the diagnosis, especially in those cases lacking definite radiological findings