478 research outputs found

    Crystallographic dissection of the spliceosomal snRNP structures

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    The Dynamics of Poor Systems of Galaxies

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    We assemble and observe a sample of poor galaxy systems that is suitable for testing N-body simulations of hierarchical clustering (Navarro, Frenk, & White 1997; NFW) and other dynamical halo models (e.g., Hernquist 1990). We (1) determine the parameters of the density profile rho(r) and the velocity dispersion profile sigma(R), (2) separate emission-line galaxies from absorption-line galaxies, examining the model parameters and as a function of spectroscopic type, and (3) for the best-behaved subsample, constrain the velocity anisotropy parameter, beta, which determines the shapes of the galaxy orbits. The NFW universal profile and the Hernquist (1990) model both provide good descriptions of the spatial data. In most cases an isothermal sphere is ruled out. Systems with declining sigma(R) are well-matched by theoretical profiles in which the star-forming galaxies have predominantly radial orbits (beta > 0); many of these galaxies are probably falling in for the first time. There is significant evidence for spatial segregation of the spectroscopic classes regardless of sigma(R).Comment: 36 pages, 20 figures, and 5 tables. To appear in the Astrophysical Journa

    Stellar structure and compact objects before 1940: Towards relativistic astrophysics

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    Since the mid-1920s, different strands of research used stars as "physics laboratories" for investigating the nature of matter under extreme densities and pressures, impossible to realize on Earth. To trace this process this paper is following the evolution of the concept of a dense core in stars, which was important both for an understanding of stellar evolution and as a testing ground for the fast-evolving field of nuclear physics. In spite of the divide between physicists and astrophysicists, some key actors working in the cross-fertilized soil of overlapping but different scientific cultures formulated models and tentative theories that gradually evolved into more realistic and structured astrophysical objects. These investigations culminated in the first contact with general relativity in 1939, when J. Robert Oppenheimer and his students George Volkoff and Hartland Snyder systematically applied the theory to the dense core of a collapsing neutron star. This pioneering application of Einstein's theory to an astrophysical compact object can be regarded as a milestone in the path eventually leading to the emergence of relativistic astrophysics in the early 1960s.Comment: 83 pages, 4 figures, submitted to the European Physical Journal

    Chaotic scattering on surfaces and collisional damping of collective modes

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    The damping of hot giant dipole resonances is investigated. The contribution of surface scattering is compared with the contribution from interparticle collisions. A unified response function is presented which includes surface damping as well as collisional damping. The surface damping enters the response via the Lyapunov exponent and the collisional damping via the relaxation time. The former is calculated for different shape deformations of quadrupole and octupole type. The surface as well as the collisional contribution each reproduce almost the experimental value, therefore we propose a proper weighting between both contributions related to their relative occurrence due to collision frequencies between particles and of particles with the surface. We find that for low and high temperatures the collisional contribution dominates whereas the surface damping is dominant around the temperatures 3/2π\sqrt{3}/2\pi of the centroid energy.Comment: PRC su

    Analysis and Exploration of Novel Antibiotic-Producing Streptomyces spp. in Spokane County, Washington

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    According to the Centers for Disease Control and Prevention, a US citizen is infected by an antibiotic-resistant pathogen every 11 seconds, and every 15 minutes, a patient dies as a result of these infections. Due to the increasing incidence of antibiotic-resistant pathogenic microbes, the study and exploration of novel antibiotics from novel environments are imperative as infectious diseases are the second leading cause of death in the United States. The purpose of this research is to investigate and analyze antibiotic-producing soil microbes in Spokane County, WA, with hopes of discovering novel antibiotic-producing microbes, specifically Streptomyces species, and explore some of the variables that influence the production of secondary metabolites. My hypotheses are as follows: Soil microbes existing in Spokane County will include Streptomyces spp. capable of producing secondary metabolites suitable to combat selected Gram-negative or Gram-positive bacterial ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) and pathogenic fungi such as Candida albicans. Additionally, modifying laboratory variables such as incubation temperature, time in incubation, and the type of media will influence the production of metabolites produced by Streptomyces isolates. Modifying these variables will impact the inhibitory capabilities of these isolates against Gram-negative, Gram-positive, and pathogenic fungal microbes. Cell-free supernatants of secondary metabolites on disk diffusion and 96 well plate assays will be utilized to measure zones of inhibition and inhibitory capabilities with absorbance measured at 600nm using a spectrophotometer

    Using “Omics” and Integrated Multi-Omics Approaches to Guide Probiotic Selection to Mitigate Chytridiomycosis and Other Emerging Infectious Diseases

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    Emerging infectious diseases in wildlife are responsible for massive population declines. In amphibians, chytridiomycosis caused by Batrachochytrium dendrobatidis, Bd, has severely affected many amphibian populations and species around the world. One promising management strategy is probiotic bioaugmentation of antifungal bacteria on amphibian skin. In vivo experimental trials using bioaugmentation strategies have had mixed results, and therefore a more informed strategy is needed to select successful probiotic candidates. Metagenomic, transcriptomic, and metabolomic methods, colloquially called “omics,” are approaches that can better inform probiotic selection and optimize selection protocols. The integration of multiple omic data using bioinformatic and statistical tools and in silico models that link bacterial community structure with bacterial defensive function can allow the identification of species involved in pathogen inhibition. We recommend using 16S rRNA gene amplicon sequencing and methods such as indicator species analysis, the Kolmogorov–Smirnov Measure, and co-occurrence networks to identify bacteria that are associated with pathogen resistance in field surveys and experimental trials. In addition to 16S amplicon sequencing, we recommend approaches that give insight into symbiont function such as shotgun metagenomics, metatranscriptomics, or metabolomics to maximize the probability of finding effective probiotic candidates, which can then be isolated in culture and tested in persistence and clinical trials. An effective mitigation strategy to ameliorate chytridiomycosis and other emerging infectious diseases is necessary; the advancement of omic methods and the integration of multiple omic data provide a promising avenue toward conservation of imperiled species

    Radio emission of Shakhbazian Compact Galaxy Groups

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    Three hundred fifty three radio sources from the NRAO VLA Sky Survey (NVSS) (Condon et al. 1998) and the FIRST Survey (White et al. 1997}, are detected in the areas of 179 Shakhbazian Compact Groups (ShCGs) of galaxies. Ninety three of them are identified with galaxies in 74 ShCGs. Six radio sources have complex structure. The radio spectra of 22 sources are determined. Radio luminosities of galaxies in ShCGs are in general higher than that of galaxies in Hickson Compact Groups (HCGs). The comparison of radio (at 1.4 GHz) and FIR (at 60 μ\mum) fluxes of ShCG galaxies with that of HCG galaxies shows that galaxies in ShCGs are relatively stronger emitters at radio wavelengths, while galaxies in HCGs have relatively stronger FIR emission. The reasons of such difference is discussed.Comment: 35 pages, 6 Postscript figures, ApJS in pres

    Discovery of Calcium, Indium, Tin, and Platinum Isotopes

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    Currently, twenty-four calcium, thirty-eight indium, thirty-eight tin and thirty-nine platinum isotopes have been observed and the discovery of these isotopes is discussed here. For each isotope a brief synopsis of the first refereed publication, including the production and identification method, is presented.Comment: to be published in At. Data Nuclear Data Tables, This updated paper combines manuscripts: 1004.4934 (Calcium), 1004.5266 (Indium), 1003.5127 (Tin), and 1006.4033 (Platinum

    A retrospective comparative study of multiple choice questions versus short answer questions as assessment tool in evaluating the performance of the students in medical pharmacology

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    Background: The aim was to assess the effectiveness of multiple choice versus short answer questions (SAQs) as assessment tools for evaluating performance of 2nd MBBS students.Methods: The study was observational, retrospective study of written pen and paper type assessment that utilized a sample of 100 2nd year medical students. Study consisted of two parts; part I was multiple-choice questions (MCQs) based on endocrine system where four options were given for a question and the single best answer was to be ticked. MCQ was timed at 20 mins for 30 questions with 1 mark each. There was no negative marking. Part II was SAQ on same system where 16 SAQ were given. SAQ was timed at 60 mins for 30 marks questions. After the test students had to reply on a feedback form.Result: There was a strong correlation between the marks scored in two formats of test and there was no statistical difference between the two set of marks.Conclusion: SAQs are as effective as MCQs in assessing the performance of the students in medical pharmacology

    Ultrafast isomerization initiated by X-ray core ionization

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    Rapid proton migration is a key process in hydrocarbon photochemistry. Charge migration and subsequent proton motion can mitigate radiation damage when heavier atoms absorb X-rays. If rapid enough, this can improve the fidelity of diffract-before-destroy measurements of biomolecular structure at X-ray-free electron lasers. Here we study X-ray-initiated isomerization of acetylene, a model for proton dynamics in hydrocarbons. Our time-resolved measurements capture the transient motion of protons following X-ray ionization of carbon K-shell electrons. We Coulomb-explode the molecule with a second precisely delayed X-ray pulse and then record all the fragment momenta. These snapshots at different delays are combined into a ‘molecular movie’ of the evolving molecule, which shows substantial proton redistribution within the first 12 fs. We conclude that significant proton motion occurs on a timescale comparable to the Auger relaxation that refills the K-shell vacancy
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