Bioaffinity detection of pathogens on surfaces

The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based detection platforms are particularly attractive as multiple bioaffinity interactions between different targets and corresponding probe molecules can be monitored simultaneously in a single measurement. Furthermore, the possibilities for developing new signal transduction mechanisms alongside novel signal amplification strategies aremuchmore varied. In this article, we describe some of the latest advances in the use of surface bioaffinity detection of pathogens. Three major sections will be discussed: (i) a brief overview on the choice of probe molecules such as antibodies, proteins and aptamers specific to pathogens and surface attachment chemistries to immobilize those probes onto various substrates, (ii) highlighting examples among the current generation of surface biosensors, and (iii) exploring emerging technologies that are highly promising and likely to form the basis of the next generation of pathogenic sensors

PATENTS, R&D AND LAG EFFECTS: EVIDENCE FROM FLEXIBLE METHODS FOR COUNT PANEL DATA ON MANUFACTURING FIRMS

Hausman, Hall and Griliches (1984) and Hall, Griliches and Hausman (1986) investigated whether there was a lag in the patent-R&D relationship for the U.S. manufacturing sector using 1970¿s data. They found that there was little evidence of anything but contemporaneous movement of patents and R&D. We reexamine this important issue employing new longitudinal patent data at the firm level for the U.S. manufacturing sector from 1982 to 1992. To address unique features of the data, we estimate various distributed lag and dynamic multiplicative panel count data models. The paper also develops a new class of count panel data models based on series expansion of the distribution of individual effects. The empirical analyses show that, although results are somewhat sensitive to different estimation methods, the contemporaneous relationship between patenting and R&D expenditures continues to be rather strong, accounting for over 60% of the total R&D elasticity. Regarding the lag structure of the patents-R&D relationship, we do find a significant lag in all empirical specifications. Moreover, the estimated lag effects are higher than have previously been found, suggesting that the contribution of R&D history to current patenting has increased from the 1970¿s to the 1980¿s.Innovative activity, Patents and R&D, Individual effects, count panel data methods.

Self-assembly of gold supraparticles with crystallographically aligned and strongly coupled nanoparticle building blocks for SERS and photothermal therapy

A new method is introduced for self-assembling citrate-capped gold nanoparticles into supraparticles with crystallographically aligned building blocks. It consists in confining gold nanoparticles inside a cellulos acetate membrane. The constituent nanoparticles are in close contact in the superstructure, and therefore generate hot spots leading to intense SERS signals. They also generate more plasmonic heat than the nanoparticle building blocks. The supraparticles are internalized by cells and show low cytotoxicity, but can kill cancer cells when irradiated with a laser. This, along with the improved plasmonic properties arising from their assembly, makes the gold supraparticles promising materials for applications in bioimaging and nanomedicine

Plastic Deformation in Laser-Induced Shock Compression of Monocrystalline Copper

Copper monocrystals were subjected to shock compression at pressures of 10–60 GPa by a short (3 ns initial) duration laser pulse. Transmission electron microscopy revealed features consistent with previous observations of shock-compressed copper, albeit at pulse durations in the µs regime. The results suggest that the defect structure is generated at the shock front. A mechanism for dislocation generation is presented, providing a realistic prediction of dislocation density as a function of pressure. The threshold stress for deformation twinning in shock compression is calculated from the constitutive equations for slip, twinning, and the Swegle-Grady relationship

A Coordinated Radio Afterglow Program

We describe a ground-based effort to find and study afterglows at centimeter and millimeter wavelengths. We have observed all well-localized gamma-ray bursts in the Northern and Southern sky since BeppoSAX first started providing rapid positions in early 1997. Of the 23 GRBs for which X-ray afterglows have been detected, 10 have optical afterglows and 9 have radio afterglows. A growing number of GRBs have both X-ray and radio afterglows but lack a corresponding optical afterglow.Comment: To appear in Proc. of the 5th Huntsville Gamma-Ray Burst Symposium, 5 pages, LaTe

Moral reasoning and homosexuality: the acceptability of arguments about lesbian and gay issues

In the political arena, lesbian and gay issues have typically been contested on grounds of human rights, but with variable success. Using a moral developmental framework, the purpose of this study was to explore preferences for different types of moral arguments when thinking about moral dilemmas around lesbian and gay issues. The analysis presented here comprised data collected from 545 students at UK universities, who completed a questionnaire, part of which comprised a moral dilemma task. Findings of the study showed that respondents do not apply moral reasoning consistently, and do not (clearly) favour human rights reasoning when thinking about lesbian and gay issues. Respondents tended to favour reasoning supporting existing social structures and frameworks, therefore this study highlights the importance of structural change in effecting widespread attitude change in relation to lesbian and gay rights issues. The implications of the findings for moral education are also discussed.</p

Adaptive response and enlargement of dynamic range

Many membrane channels and receptors exhibit adaptive, or desensitized, response to a strong sustained input stimulus, often supported by protein activity-dependent inactivation. Adaptive response is thought to be related to various cellular functions such as homeostasis and enlargement of dynamic range by background compensation. Here we study the quantitative relation between adaptive response and background compensation within a modeling framework. We show that any particular type of adaptive response is neither sufficient nor necessary for adaptive enlargement of dynamic range. In particular a precise adaptive response, where system activity is maintained at a constant level at steady state, does not ensure a large dynamic range neither in input signal nor in system output. A general mechanism for input dynamic range enlargement can come about from the activity-dependent modulation of protein responsiveness by multiple biochemical modification, regardless of the type of adaptive response it induces. Therefore hierarchical biochemical processes such as methylation and phosphorylation are natural candidates to induce this property in signaling systems.Comment: Corrected typos, minor text revision

The γ-ray burst of 980425 and its association with the extraordinary radio emission from a most unusual supernova

Supernova SN 1998bw exploded in the same direction and at the same time as the gamma-ray burst GRB 980425. Here we report radio observations of this type Ic supernova, beginning 4 days after the gamma-ray burst. At its peak the radio source is the most luminous ever seen from a supernova, νL_ν = 4 x 10^(38) erg/s at 5 GHz. More remarkably, the traditional synchrotron interpretation of the radio emission requires that the radio source be expanding at an apparent velocity of at least twice the speed of light, indicating that this supernova was accompanied by a shock wave moving at relativistic speed. The energy U_e associated with the radio-emitting relativistic electrons must lie between 10^(49) erg < U_e < 10^(52) erg, and thus represents a significant fraction of the total kinetic energy ~10^(51) erg associated with supernova explosions. The presence of a relativistic shock in SN 1998bw suggests a physical connection with the gamma-ray burst GRB 980425. We argue that this represents a second class of gamma-ray burst, with much lower emitted energy ~10^(48) erg in gamma-rays than the two powerful ~10^(53) erg high-redshift gamma-ray bursts previously identified