THE 23 JULY 1930 EARTHQUAKE (MS=6.7) IN THE SOUTHERN APENNINES (ITALY): GEOLOGICAL AND HYDROLOGICAL EFFECTS

The 23 July 1930 earthquake (MS=6.7) in the Southern Apennines (Italy) was a catastrophic event that produced many effects such as surface faulting, fractures, landslides, settlements, hydrological changes, variations in chemical/ physical activity related to the volcanic and/or thermal zones and also acoustic and optical phenomena. It is the first great earthquake of the twentieth century that was studied, thanks to the hydrological monitoring network of the Italian Hydrographic Survey (IHS) set up from 1925 to 1929. For this earthquake we analysed the initial IHS hydrometric and pluviometric data, looking for significant anomalies in springs, water wells and mountain streams. Hydrological data relative to rivers, springs and water wells indicate that some changes can be correlated with the earthquake: a post-seismic excess discharge in some streams, pre- and co-seismic decreases in stream flows and water levels in wells, pre- and post-seismic increases in discharges. The pre- and co-seismic stresses and the tectonic deformations were studied in order to find a possible model of interaction between stress state and hydrological variations. The anomalies found in this work can be considered “rebound anomalies”, which are the most common precursor reported by several authors and related to increases in porosity and permeability caused by the fracturing that precedes an earthquake. An estimation of the total excess discharge (0.035 km3) caused by the Ms=6.7 Irpinia earthquake is consistent with the excess discharge of about 0.01 km3 determined for the Mw=6.9 Loma Prieta earthquake. To define the normal hydrodynamic behaviour of the considered aquifers, an analysis of the correlation between rainfalls and water levels and flow rates has been carried out. The delay of significant peaks in the correlograms of figure 7 with respect to the start of the hydrological anomaly and/or its duration, can define the space-time limits of the changes correlated with earthquake

Hydrological anomalies connected to earthquakes in southern Apennines (Italy)

The study of hydrological variations in the watersheds of seismic areas can be useful in order to acquire a new knowledge of the mechanisms by which earthquakes can produce hydrological anomalies. Italy has the availability of many long historical series both of hydrological parameters and of seismological data, and is an ideal laboratory to verify the validity of theoretical models proposed by various authors. In this work we analyse the hydrological anomalies associated with some of the big earthquakes that occurred in the last century in the southern Apennines: 1930, 1980 and 1984. For these earthquakes we analysed hydrometric and pluviometric data looking for significant anomalies in springs, water wells and mountain streams. The influence of rainfalls on the normal flows of rivers, springs and wells has been ascertained. Also, the earthquake of 1805, for which a lot of hydrological perturbations have been reported, is considered in order to point out effects imputable to this earthquake that can be similar to the effects of the other big earthquakes. The considered seismic events exhibit different modes of energy release, different focal mechanisms and different propagation of effects on the invested areas. Furthermore, even if their epicentres were not localised in contiguous seismogenetic areas, it seems that the hydrological effects imputable to them took place in the same areas. Such phenomena have been compared with macroseismic fields and transformed in parameters, in order to derive empirical relationships between the dimensions of the event and the characteristics of the hydrological variations. The results of this work point to a close dependence among hydrological anomalies, regional structures and fault mechanisms, and indicate that many clear anomalies have been forerunners of earthquakes. In 1993, the Naples Bureau of the Hydrographic National Service started the continuous monitoring of hydrologic parameters by a network of automatic stations and transmission in real time; presently 7 acquifers are under control in which also pH, <i>T</i> , salinity, electrical conductivity, dissolved oxygen are measured. We envisage to increase the number of monitoring sites and controlled parameters

Cataract and optic disk drusen in a patient with glycogenosis and di George syndrome: clinical and molecular report

Background We report the ophthalmic findings of a patient with type Ia glycogen storage disease (GSD Ia), DiGeorge syndrome (DGS), cataract and optic nerve head drusen (ONHD). Case presentation A 26-year-old white woman, born at term by natural delivery presented with a post-natal diagnosis of GSD Ia. Genetic testing by array-comparative genomic hybridization (CGH) for DGS was required because of her low levels of serum calcium. The patient has been followed from birth, attending the day-hospital every six months at the San Paolo Hospital, Milan, outpatient clinic for metabolic diseases and previously at another eye center. During the last day-hospital visit, a complete eye examination showed ONHD and cataract in both eyes. Next Generation Sequencing (NGS) was subsequently done to check for any association between the eye problems and metabolic aspects. Conclusions This is the first description of ocular changes in a patient with GSD Ia and DGS. Mutations explaining GSD Ia and DGS were found but no specific causative mutation for cataract and ONHD. The metabolic etiology of her lens changes is known, whereas the pathogenesis of ONHD is not clear. Although the presence of cataract and ONHD could be a coincidence; the case reported could suggest that hypocalcemia due to DGS could be the common biochemical pathway

Exon 3 of the NUMB Gene Emerged in the Chordate Lineage Coopting the NUMB Protein to the Regulation of MDM2

MDM2 regulates a variety of cellular processes through its dual protein:protein interaction and ubiquitin ligase activities. One major function of MDM2 is to bind and ubiquitinate P53, thereby regulating its proteasomal degradation. This function is in turn controlled by the cell fate determinant NUMB, which binds to and inhibits MDM2 via a short stretch of 11 amino acids, contained in its phosphotyrosine-binding (PTB) domain, encoded by exon 3 of the NUMB gene. The NUMB-MDM2-P53 circuitry is relevant to the specification of the stem cell fate and its subversion has been shown to be causal in breast cancer leading to the emergence of cancer stem cells. While extensive work on the evolutionary aspects of the MDM2/P53 circuitry has provided hints as to how these two proteins have evolved together to maintain conserved and linked functions, little is known about the evolution of the NUMB gene and, in particular, how it developed the ability to regulate MDM2 function. Here, we show that NUMB is a metazoan gene, which acquired exon 3 in the common ancestor of the Chordate lineage, first being present in the Cephalochordate and Tunicate subphyla, but absent in invertebrates. We provide experimental evidence showing that since its emergence, exon 3 conferred to the PTB domain of NUMB the ability to bind and to regulate MDM2 functions

Mice lacking endoglin in macrophages show an impaired immune response

24 p.-9 fig.-1 tab. Ojeda Fernández, Luisa et al.Endoglin is an auxiliary receptor for members of the TGF-β superfamily and plays an important role in the homeostasis of the vessel wall. Mutations in endoglin gene (ENG) or in the closely related TGF-β receptor type I ACVRL1/ALK1 are responsible for a rare dominant vascular dysplasia, the Hereditary Hemorrhagic Telangiectasia (HHT), or Rendu-OslerWeber syndrome. Endoglin is also expressed in human macrophages, but its role in macrophage function remains unknown. In this work, we show that endoglin expression is triggered during the monocyte-macrophage differentiation process, both in vitro and during the in vivo differentiation of blood monocytes recruited to foci of inflammation in wild-type C57BL/6 mice. To analyze the role of endoglin in macrophages in vivo, an endoglin myeloid lineage specific knock-out mouse line (Engfl/flLysMCre) was generated. These mice show a predisposition to develop spontaneous infections by opportunistic bacteria. Engfl/flLysMCre mice also display increased survival following LPS-induced peritonitis, suggesting a delayed immune response. Phagocytic activity is impaired in peritoneal macrophages, altering one of the main functions of macrophages which contributes to the initiation of the immune response. We also observed altered expression of TGF-β1 target genes in endoglin deficient peritoneal macrophages. Overall, the altered immune activity of endoglin deficient macrophages could help to explain the higher rate of infectious diseases seen in HHT1 patients.This work was funded by: Ministerio de Economía y Competitividad of Spain (SAF2011-23475 to LMB; SAF2013-43421-R and SAF2010- 19222 to CB.Peer reviewe

Proteolysis, Texture and Microstructure of Goat Cheese

Changes in the structure of cheese are mostly due to changes in the protein matrix, mainly because of the degradation of α- and β- and k-casein. Therefore, the objective of this work was to study the effect of proteolysis on the microstructure and texture of goat cheese during ripening. The cheeses were made using Creole goat milk from the Quebrada de Humahuaca in Jujuy and ripened at 10 °C and 90% RH. Samples were taken after 5 hours of preparation and after 10, 20, 30, 40, 60 and 80 days of ripening. Proteolysis was studied by the evolution of the major fractions of casein (α, β and para-κ) determined by HPLC and soluble nitrogen, allowing the calculation of the rate of maturation. The texture profile was determined using a texture analyzer QTS 25. The changes in the protein matrix of the cheese were observed by scanning electron microscopy using a JEOL JSM-6480 LV. We found that the α-casein was hydrolyzed at a low speed at the beginning and until 30 days, between 30 and 40 days of ripening, α-casein was hydrolyzed faster. After this time, this fraction content became stable until the end of the ripening. The rate of hydrolysis of para-κ-casein increased starting from 30 days up to 60 days of ripening, when it became stable. It was observed that the initial matrix of cheese protein was formed by free large cavities with a heterogeneous dispersion of casein particles. During ripening, the size of the cavities decreased and the cheese protein matrix became more compact. The size of the holes was reduced and the globular characteristic of the micelles was lost after 40 days of maturation, coinciding with accentuated hydrolysis α-caseins. The soluble nitrogen at pH 4.6, increased significantly until 30 days. After that, it remained statistically unchanged for 80 days. The velocity of maturation determined as soluble nitrogen in TCA, rose steadily until 60 days of ripening. Hardness, gumminess, adhesiveness and chewiness increased sharply at 40 days of maturation. After this time, these parameters increased slowly until the end of the sampling period, when the changes in the microstructure of the cheeses revealed the highest compaction of the matrix. This may be related to the formation of soluble nitrogen and degradation of α-caseins during ripeningFil: Burgos, Laura Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Jujuy. Facultad de Ingeniería. Centro de Investigación en Tecnología Alimentaria; ArgentinaFil: Pece Azar, Nora Beatriz del Carmen. No especifíca;Fil: Maldonado, S.. No especifíca

NUMB (numb homolog (Drosophila))

Review on NUMB (numb homolog (Drosophila)), with data on DNA, on the protein encoded, and where the gene is implicated

Cancer stem cell markers in breast cancer: pathological, clinical and prognostic significance

INTRODUCTION: The cancer stem cell (CSC) hypothesis states that tumours consist of a cellular hierarchy with CSCs at the apex driving tumour recurrence and metastasis. Hence, CSCs are potentially of profound clinical importance. We set out to establish the clinical relevance of breast CSC markers by profiling a large cohort of breast tumours in tissue microarrays (TMAs) using immunohistochemistry (IHC). METHODS: We included 4, 125 patients enrolled in the SEARCH population-based study with tumours represented in TMAs and classified into molecular subtype according to a validated IHC-based five-marker scheme. IHC was used to detect CD44/CD24, ALDH1A1, aldehyde dehydrogenase family 1 member A3 (ALDH1A3) and integrin alpha-6 (ITGA6). A 'Total CSC' score representing expression of all four CSC markers was also investigated. Association with breast cancer specific survival (BCSS) at 10 years was assessed using a Cox proportional-hazards model. This study was complied with REMARK criteria. RESULTS: In ER negative cases, multivariate analysis showed that ITGA6 was an independent prognostic factor with a time-dependent effect restricted to the first two years of follow-up (hazard ratio (HR) for 0 to 2 years follow-up, 2.4; 95% confidence interval (95% CI), 1.2 to 4.8; P = 0.009). The composite 'Total CSC' score carried independent prognostic significance in ER negative cases for the first four years of follow-up (HR for 0 to 4 years follow-up, 1.3; 95% CI, 1.1 to 1.6; P = 0.006). CONCLUSIONS: Breast CSC markers do not identify identical subpopulations in primary tumours. Both ITGA6 and a composite Total CSC score show independent prognostic significance in ER negative disease. The use of multiple markers to identify tumours enriched for CSCs has the greatest prognostic value. In the absence of more specific markers, we propose that the effective translation of the CSC hypothesis into patient benefit will necessitate the use of a panel of markers to robustly identify tumours enriched for CSCs