47 research outputs found

    A multi-center peptidomic investigation of simulated gastrointestinal food digestion

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    Background: Mass spectrometry has become the techniques of choice for the simultaneous assessment of a high variety of molecules in complex matrices. For this reason, it is best suited for precisely monitoring the evolution of digestive processes in vivo and in vitro. However, due to the variety of equipment available in different laboratories as well as settings, statistical evaluations, and interpretations, it is difficult to predict a priori the ideal parameters for best results. Methods: The present work addressed that gap by executing an inter-laboratory study with samples collected during in vitro digestion and presenting an overview of the state of the art mass spectrometry applications and analytical capabilities available for studying food digestion. Results: Three representative high-protein foods, skim milk powder, chicken breast and tofu, were digested according to the INFOGEST protocol with sample collection at five time points during gastric and intestinal digestion. Nine laboratories analyzed all digesta with their in house equipment, conventional sample treatment procedure, data evaluation methods, and graphical representation tools. Conclusions: The compiled results demonstrate a strong consensus aming labs in terms of major protein degradation for the three foods matrices and present suitable methodological and statistical approaches for representing the analytical results

    Como poderia a Gerontologia, um campo multidisciplinar do saber, estar presente na Tabela das Áreas do Conhecimento do CNPq? How could Gerontology, a multidisciplinary field of knowledge, be included in CNPq's Table of Knowledge Areas?

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    Discutimos a possibilidade da inserção da Gerontologia na Tabela das Áreas do Conhecimento do CNPq num cenário em essa tabela vem sendo questionada na comunidade científica, particularmente no que se refere à inclusão de áreas multidisciplinares. A partir de Foucault, entendemos a Árvore do Conhecimento como uma taxonomia, um continuum, onde todas as áreas são colocadas lado a lado, mais próximas ou mais afastadas conforme semelhanças e diferenças entre si. Trata-se de um tratamento linear e finito que estabelece que uma determinada área do conhecimento só pode estar situada num ponto da parte da linha que corresponde a uma grande área. A Gerontologia caracteristicamente multidisciplinar não alcançou lugar nessa taxonomia institucionalizada, seja porque haveria problemas em relação a conceitos, interesses e projeto político em sua constituição como área do conhecimento, seja porque a taxonomia seria incompatível com a multidisciplinaridade. Concluímos que é possível conceber uma nova Tabela de Campos de Conhecimentos e de Saberes e proceder visualizações dos estudos sobre o envelhecimento e sobre toda e qualquer região dos conhecimentos e dos saberes na plenitude de sua muldisciplinaridade e de suas transformações ao longo dos tempos.<br>We discuss the possibility of including Gerontology in the CNPq Areas of Knowledge Table, in a scenario where this Table is being questioned by the scientific community, particularly with regard to the inclusion of multidisciplinary areas. Based on Foucault, we view the Tree of Knowledge as taxonomy, a continuum in which all areas are placed side by side, closer together or further apart, depending on their similarities and differences. This finite linear approach establishes that a certain Area of knowledge may be placed only at a point along the line corresponding to a Greater Area. Inherently multidisciplinary, gerontology has not been placed in this institutionalized taxonomy, due either to problems related to concepts, interests and political projects in its establishment as a field of knowledge, or because this taxonomy would be not be compatible with its multidisciplinary nature. We conclude that it is possible to draw up a new Fields of Knowledge and Expertise Table, addressing studies of aging - as well as all and any Fields of Knowledge and Expertise - in their full multidisciplinarity, together with their transformations over time
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