Effect of synthetic and algal astaxanthin levels on egg astaxanthin content of rainbow trout (Oncorhynchus mykiss)

This research was done in a trout farm in Kohkiloyeh and Boyer- Ahmad province. The main object of this study was comparing of two sources of astaxanthin (synthetic or algae) in feed on astaxanthin content of egg in rainbow trout. It was considered seven groups consisting six treatments (T1-T6) in two different astaxanthin sources and a control (C) (without astaxanthin). So, algal astaxanthin (haematococcus pluvialis) in the three levels of 2.67, 3.55 and 8gr/kg food (T1,T2, T3); and synthetic astaxanthin in three levels of 40, 80 and 120mg/kg food in diet (T4, T5, T6) examined on 140 trout broods (3-4 years) for 4 months, before the spawning season. Astaxanthin content of obtained eggs from all treatments in spawning season was measured by HPLC apparatus. The highest and the lowest amount of egg astaxanthin were observed in T3 and C respectively. In each astaxanthin group, a significant difference was obtained between averages in treatments (P <0.05), as T3 was the highest between them. No significant difference was observed between synthetic astaxanthin treatments (T4, T5 and T6) and T1 (the lowest level of algal astaxanthin). Treatments T2 and T6 also had the same function in term of saving astaxanthin in eggs. It also concluded that natural astaxanthin (Haematococcus pluvialis) for the reason that contains supplementary nutritious, is extraordinary preferable than synthetic astaxanthin to improve astaxanthin content of egg in rainbow trout

The breadth of primary care: a systematic literature review of its core dimensions

Background: Even though there is general agreement that primary care is the linchpin of effective health care delivery, to date no efforts have been made to systematically review the scientific evidence supporting this supposition. The aim of this study was to examine the breadth of primary care by identifying its core dimensions and to assess the evidence for their interrelations and their relevance to outcomes at (primary) health system level. Methods: A systematic review of the primary care literature was carried out, restricted to English language journals reporting original research or systematic reviews. Studies published between 2003 and July 2008 were searched in MEDLINE, Embase, Cochrane Library, CINAHL, King's Fund Database, IDEAS Database, and EconLit. Results: Eighty-five studies were identified. This review was able to provide insight in the complexity of primary care as a multidimensional system, by identifying ten core dimensions that constitute a primary care system. The structure of a primary care system consists of three dimensions: 1. governance; 2. economic conditions; and 3. workforce development. The primary care process is determined by four dimensions: 4. access; 5. continuity of care; 6. coordination of care; and 7. comprehensiveness of care. The outcome of a primary care system includes three dimensions: 8. quality of care; 9. efficiency care; and 10. equity in health. There is a considerable evidence base showing that primary care contributes through its dimensions to overall health system performance and health. Conclusions: A primary care system can be defined and approached as a multidimensional system contributing to overall health system performance and health

Combined search for the quarks of a sequential fourth generation

Results are presented from a search for a fourth generation of quarks produced singly or in pairs in a data set corresponding to an integrated luminosity of 5 inverse femtobarns recorded by the CMS experiment at the LHC in 2011. A novel strategy has been developed for a combined search for quarks of the up and down type in decay channels with at least one isolated muon or electron. Limits on the mass of the fourth-generation quarks and the relevant Cabibbo-Kobayashi-Maskawa matrix elements are derived in the context of a simple extension of the standard model with a sequential fourth generation of fermions. The existence of mass-degenerate fourth-generation quarks with masses below 685 GeV is excluded at 95% confidence level for minimal off-diagonal mixing between the third- and the fourth-generation quarks. With a mass difference of 25 GeV between the quark masses, the obtained limit on the masses of the fourth-generation quarks shifts by about +/- 20 GeV. These results significantly reduce the allowed parameter space for a fourth generation of fermions.Comment: Replaced with published version. Added journal reference and DO

Enzyme immobilisation on amino-functionalised multi-walled carbon nanotubes : structural and biocatalytic characterisation

BACKGROUND: The aim of this work is to investigate the structure and function of enzymes immobilised on nanomaterials. This work will allow better understanding of enzyme-nanomaterial interactions, as well as designing functional protein-nanomaterial conjugates. METHODOLOGY/PRINCIPAL FINDINGS: Multiwalled carbon nanotubes (MWNTs) were functionalised with amino groups to improve solubility and biocompatibility. The pristine and functionalised forms of MWNTs were characterised with Fourier-transform infrared spectroscopy. Thermogravimetric analysis was done to examine the degree of the functionalisation process. An immobilised biocatalyst was prepared on functionalised nanomaterial by covalent binding. Thermomyces lanuginosus lipase was used as a model enzyme. The structural change of the immobilised and free lipases were characterised with transmission electron Microscopy, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy and Circular dichroism spectroscopy. Biochemical characterisation of immobilised enzyme showed broader pH and thermal optima compared to soluble form. Reusability of the immobilised enzyme for hydrolysis of long chain esters was demonstrated up to ten cycles. CONCLUSION/SIGNIFICANCE: Lipase immobilised on MWNTs has exhibited significantly improved thermal stability. The exploration of advanced nanomaterial for enzyme immobilisation support using sophisticated techniques makes nanobiocatalyst of potential interest for biosensor applications

Value of hospital antimicrobial stewardship programs [ASPs]:a systematic review

Abstract Background Hospital antimicrobial stewardship programs (ASPs) aim to promote judicious use of antimicrobials to combat antimicrobial resistance. For ASPs to be developed, adopted, and implemented, an economic value assessment is essential. Few studies demonstrate the cost-effectiveness of ASPs. This systematic review aimed to evaluate the economic and clinical impact of ASPs. Methods An update to the Dik et al. systematic review (2000–2014) was conducted on EMBASE and Medline using PRISMA guidelines. The updated search was limited to primary research studies in English (30 September 2014–31 December 2017) that evaluated patient and/or economic outcomes after implementation of hospital ASPs including length of stay (LOS), antimicrobial use, and total (including operational and implementation) costs. Results One hundred forty-six studies meeting inclusion criteria were included. The majority of these studies were conducted within the last 5 years in North America (49%), Europe (25%), and Asia (14%), with few studies conducted in Africa (3%), South America (3%), and Australia (3%). Most studies were conducted in hospitals with 500–1000 beds and evaluated LOS and change in antibiotic expenditure, the majority of which showed a decrease in LOS (85%) and antibiotic expenditure (92%). The mean cost-savings varied by hospital size and region after implementation of ASPs. Average cost savings in US studies were $732 per patient (range:$2.50 to $2640), with similar trends exhibited in European studies. The key driver of cost savings was from reduction in LOS. Savings were higher among hospitals with comprehensive ASPs which included therapy review and antibiotic restrictions. Conclusions Our data indicates that hospital ASPs have significant value with beneficial clinical and economic impacts. More robust published data is required in terms of implementation, LOS, and overall costs so that decision-makers can make a stronger case for investing in ASPs, considering competing priorities. Such data on ASPs in lower- and middle-income countries is limited and requires urgent attention

The incidence and prevalence of delirium across palliative care settings: A systematic review

© The Author(s) 2019. Background: Delirium is a common and distressing neurocognitive condition that frequently affects patients in palliative care settings and is often underdiagnosed. Aim: Expanding on a 2013 review, this systematic review examines the incidence and prevalence of delirium across all palliative care settings. Design: This systematic review and meta-analyses were prospectively registered with PROSPERO and included a risk of bias assessment. Data sources: Five electronic databases were examined for primary research studies published between 1980 and 2018. Studies on adult, non-intensive care and non-postoperative populations, either receiving or eligible to receive palliative care, underwent dual reviewer screening and data extraction. Studies using standardized delirium diagnostic criteria or valid assessment tools were included. Results: Following initial screening of 2596 records, and full-text screening of 153 papers, 42 studies were included. Patient populations diagnosed with predominantly cancer (n = 34) and mixed diagnoses (n = 8) were represented. Delirium point prevalence estimates were 4%–12% in the community, 9%–57% across hospital palliative care consultative services, and 6%–74% in inpatient palliative care units. The prevalence of delirium prior to death across all palliative care settings (n = 8) was 42%–88%. Pooled point prevalence on admission to inpatient palliative care units was 35% (confidence interval = 0.29–0.40, n = 14). Only one study had an overall low risk of bias. Varying delirium screening and diagnostic practices were used. Conclusion: Delirium is prevalent across all palliative care settings, with one-third of patients delirious at the time of admission to inpatient palliative care. Study heterogeneity limits meta-analyses and highlights the future need for rigorous studies

Kewajiban Kepala Desa Sebagai Mediator Dalam Menyelesaikan Perselisihan Hak Atas Tanah Masyarakat Desa

Penyelenggara pemerintah desa dilaksanakan oleh kepala desa disisi lain kepala desa diberi kewajiban sebagai mediator, paham yang dianut Indonesia paham distribusi kekuasaan yang intinya kekuasaan tidak bertupuk pada satu orang, jenis perselisihan dimasyarakat desa yang diselesaikan oleh kepala desa tidak jelas, meningkatnya perkara dipengadilan tentang hak atas tanah dari tahun 2014-2015, proses mediasi dipengadilan kurang maksimal. Aspek ontologi, hakikat kebenaran dan kenyataan, kebenaran penyelesaian perselisihan masyarakat desa yang kemudian penyelesaian ini dilalukan oleh Kepala Desa. Kepala Desa merupakan penyelengaran pemeritah desa untuk urusan masyarakat desa dalam bidang adminstrasi selain itu kepala Desa juga harus bertindak sebagai mediator untuk menyelesaikan perselisihan di masyarakat. Epistemologi, Secara Epistomologi penyelesaian perselisihan masyarakat desa cara yang hendak digunakan untuk mengakhri perselisihan pada masyarakat, mengunakan lembaga yang berada didesa baik itu lembaga yang sudah ada sejak lama maupun lembaga yang akan baru diadakan guna untuk mengakhiri perselisihan masyarakat secara damai. Aspek Aksiologi, mempertanyakan apakah penugasan kepala desa sebagai mediator sudah sesuai dengan tujuan hukum mengingat kepala desa memiliki dua peran yaitu sebagai penyelengarah pemerintahan desa dan sebagai mediator. Rumusan masalah 1). apakah kewajiban kepala desa sebagai mediator dalam menyelesaikan perselisihan hak atas tanah masyarakat desa 2). mengapa kepala desa diberi kewajiban sebagai mediator dalam menyelesaikan perselisihan hak atas tanah masyarakat desa 3). bagaimana pengaturan penyelesaian perselisihan hak atas tanah masyarakat desa sesuai dengan tujuan hukum Metode yang dipergunakan dalam penelitian ini adalah jenis penelitian hukum (legal research) dengan mengunakan pendekatan filsafati ( philosophical approach), konsep, kasus, sejarah dan undang-undang (statute approacht). teori dan konsep yang digunakan sebagai piasu analisis dalam disertasi ini, teori tujuan hukum, teori keadilan, teori kewenangan, teori penyelesaian sengketa. Hasil penelitian : Pertama Kewajiban Kepala Desa Sebagai Mediator, secara filosofis tujuan Negara Republik Indonesia pembukan alinea ke IV UUD NRI Tahun 1945, salah tujuan terbentuknya Negara Republik ini untuk menciptakan Perdamaian abadi, secara Yuridis untuk membina memelihara ketentraman dan ketertiban masyarakat desa, Secara Teoritis untuk mengakhir perselisihan para pihak tercipta keadaan seperti semula hidup damai. Kedua Pertimbangan kepala Desa diberi kewajiban sebagai mediator, Kepala Desa sebagai penyelenggara pemerintahan Desa, kewenangan dari Undang-undang dan berdasarkan hak asal vii usul, pemerintah Desa sangat dekat dengan masyarakat desa. Ketiga penyelesaian perselisihan hak atas tanah masyarakat desa sesuai dengan tujuan hukum, pertama penyelesaian yang berkeadilan yaitu a). kepala desa sebagai mediator b). kepala desa bersama lembaga khusus penyelesaian perselisihan c). jika kepala desa tidak digunakan sebagai mediator maka perlu adanya lembaga khusus menyelesaikan perselisihan hak atas tanah masyarakatan desa, penyelenggara LPMD tentunya harus paham hukum negara, hukum adat dan hukum agama sehingga hasil penyelesaian akan mendekati rasa keadilan. Kedua hukum yang berkepastian, penguatan hak asal usul terkait dengan penyelesaian perselisihan masyarakat desa dengan perda atau perdes dengan meperhatikan peraturan perudang-undangan lain jika diperlukan adanya pejabat khusus yang mencatat akta perdamaian masyarakat desa. Ketiga hukum yang bermanfaat, memberikan manfaat bagi masyarakat desa guna mewujudkan penyelesaian perselisihan cepat sederhana dan biaya ringan