JRC’s reference lists of MSFD species and habitats: MSFD reporting for Descriptors 1 and 6

JRC produced reference lists of species and habitats for the MSFD, following the recommendations listed in the COM DEC 2017/848/EU and shared them with the D1 experts, proposed by the Member States, to evaluate them. This task is part of the mandate to JRC (GES_18-2017-03) to facilitate Member States data entry in the EEA reporting web-forms, while increasing consistency and harmonization across Member States. The scope of the technical report is to describe the synthesis of the reference lists, to provide directions to the experts to evaluate and update them and to allow Member States to consult the reference lists (embedded in the document) for any future MSFD related task.JRC.D.2-Water and Marine Resource

A Pan-European Delimitation of Coastal Waters: Compliance with EU Environmental Legislation

The definition of coastal waters in relation to EU environmental legislation was clearly stated in the Water Framework Directive. In compliance with this Directive, most of the EU Member States delineated their coastal waters¿ boundaries. However, these delineations are not as complete and homogeneous as could be expected. A clear identification of European coastal waters boundaries is crucial for the implementation of the Water Framework Directive and the Marine Strategy Framework Directive, which depend on an accurate ecological/environmental assessment of those waters. Hence, there is a need for a comprehensive and unambiguous delimitation of European coastal waters. This report aims at bridging this gap providing a pan-European mapping of coastal waters, which cover 553,817 km2 in 30 seaside countries, 340,524 km2 of which pertain to the 22 EU Member States connected to the sea. For this purpose, a comprehensive geographical analysis of the national baselines and transitional waters distribution was performed. A pan-European baseline of 63,340 km was delineated as a basis for the European coastal waters delimitation. The European coastal waters identified in this work show significant differences with the available national declarations (almost 12% of the compared area), the latter defining an additional area of 29,337 km2 with respect to the former. The largest deviations seem to be due to misinterpretations of the definition of coastal waters in the Water Framework Directive, although a number of one-sided national modifications to that definition are also observed. This work provides the geographical basis for a full consultation process and discussion about this subject. Our recommendations include setting a clear geographical limit between the Water Framework Directive and the Marine Strategy Framework Directive jurisdiction, revise the possible exemptions in the definition of coastal waters, and discuss their consequences in the assessment of ecological/environmental status.JRC.DDG.H.5-Rural, water and ecosystem resource

Self-Assessment of Adherence to Medication: A Case Study in Campania Region Community-Dwelling Population.

Objectives. The aim of the study was to assess self-reported medication adherence measure in patients selected during a health education and health promotion focused event held in the Campania region. The study also assessed sociodemographic determinants of adherence. Methods. An interviewer assisted survey was conducted to assess adherence using the Italian version of the 8-item Morisky Medication Adherence Scale (MMAS-8). Participants older than 18 years were interviewed by pharmacists while waiting for free-medical checkup. Results. A total of 312 participants were interviewed during the Health Campus event. A total of 187 (59.9%) had low adherence to medications. Pearson's bivariate correlation showed positive association between the MMAS-8 score and gender, educational level and smoking (P < 0.05). A multivariable analysis showed that the level of education and smoking were independent predictors of adherence. Individuals with an average level of education (odds ratio (OR), 2.21, 95% confidence interval (CI), 1.08-4.52) and nonsmoker (odds ratio (OR) 1.87, 95% confidence interval (CI), 1.04-3.35) were found to be more adherent to medication than those with a lower level of education and smoking. Conclusion. The analysis showed very low prescription adherence levels in the interviewed population. The level of education was a relevant predictor associated with that result

Application of the LOICZ Methodology to the Mediterranean Sea

From a broad perspective, the LOICZ (Land-Ocean Interactions in the Coastal Zone) approach attempts to evaluate coastal change from a system perspective and assumes that the effects taking place are due to pressures within the whole basin. The LOICZ methodology was applied to the Mediterranean Sea in order to estimate its water, salt and nutrient budget, as well as to gain insights into its biogeochemical cycles. In order to undertake this budget approach, various input data relative to the three budgets were gathered for the time period of 1996 to 2005. In the case of the Mediterranean Sea, it was seen that there is still a gap between LOICZ data requirements and the standard monitoring practices in the coastal zone, both from a spatial as well as temporal viewpoint. The results show that on average over the time period 1996 to 2005, the phosphorous and nitrogen budgets of 1.35·10-2 mmol m-2 d-1 and 3.22·10-1 mmol m-2 d-1, respectively, suggest an excess of release over uptake. The budget calculations translate to the fact that on average the Mediterranean Sea acts as a sink of phosphorous and nitrogen. No specific, statistically relevant trend of the water quality and ecosystem functioning of the Mediterranean Sea was observed. An examination of the stoichiometric linkage of C, N and P through the Redfield ratio indicates that nitrogen fixation and production of dissolved inorganic carbon dominate over denitrification and consumption of dissolved inorganic carbon via net organic production.JRC.H.5-Rural, water and ecosystem resource

Comparison between soluble ST2 and high-sensitivity troponin I in predicting short-term mortality for patients presenting to the Emergency Department with chest pain

Background: High-sensitivity cardiac troponin I (hs-cTnI) and the soluble isoform of suppression of tumorigenicity 2 (sST2) are useful prognostic biomarkers in acute coronary syndrome (ACS). The aim of this study was to test the short term prognostic value of sST2 compared with hs-cTnI in patients with chest pain. Methods: Assays for hs-cTnI and sST2 were performed in 157 patients admitted to the Emergency Department (ED) for chest pain at arrival. In-hospital and 30-day follow-up mortalities were assessed. Results: The incidence of ACS was 37%; 33 patients were diagnosed with ST elevation myocardial infarction (STEMI), and 25 were diagnosed with non-ST elevation myocardial infarction (NSTEMI). Compared with the no acute coronary syndrome (NO ACS) group, the median level of hs-cTnI was higher in ACS patients: 7.22 (5.24-14) pg/mL vs 68 (15.33-163.50) pg/mL (P35 ng/mL at ED arrival died during the 30-day follow-up. Conclusions: sST2 has a greater prognostic value for 30-day cardiac mortality after discharge in patients presenting to the ED for chest pain compared with hs-cTnI. In STEMI patients, an sST2 value > 35 ng/mL at ED arrival showed the highest predictive power for short-term mortality

Lithium limits trimethyltin-induced cytotoxicity and proinflammatory response in microglia without affecting the concurrent autophagy impairment

Trimethyltin (TMT) is a highly toxic molecule present as an environmental contaminant causing neurodegeneration particularly of the limbic system both in humans and in rodents. We recently described the occurrence of impairment in the late stages of autophagy in TMT-intoxicated astrocytes. Here we show that similarly to astrocytes also in microglia, TMT induces the precocious block of autophagy indicated by the accumulation of the autophagosome marker, microtubule associated protein light chain 3. Consistent with autophagy impairment we observe in TMT-treated microglia the accumulation of p62/SQSTM1, a protein specifically degraded through this pathway. Lithium has been proved effective in limiting neurodegenerations and, in particular, in ameliorating symptoms of TMT intoxication in rodents. In our in vitro model, lithium displays a pro-survival and anti-inflammatory action reducing both cell death and the proinflammatory response of TMT-treated microglia. In particular, lithium exerts these activities without reducing TMT-induced accumulation of light chain 3 protein. In fact, the autophagic block imposed by TMT is unaffected by lithium administration. These results are of interest as defects in the execution of autophagy are frequently observed in neurodegenerative diseases and lithium is considered a promising therapeutic agent for these pathologies. Thus, it is relevant that this cation can still maintain its pro-survival and anti-inflammatory role in conditions of autophagy bloc

Impairment of the autophagic flux in astrocytes intoxicated by trimethyltin

Autophagy is a lysosomal catabolic route for protein aggregates and damaged organelles which in different stress conditions, such as starvation, generally improves cell survival. An impairment of this degradation pathway has been reported to occur in many neurodegenerative processes. Trimethyltin (TMT) is a potent neurotoxin present as an environmental contaminant causing tremors, seizures and learning impairment in intoxicated subjects. The present data show that in rat primary astrocytes autophagic vesicles (AVs) appeared after few hours of TMT treatment. The analysis of the autophagic flux in TMT-treated astrocytes was consistent with a block of the late stages of autophagy and was accompanied by a progressive accumulation of the microtubule associated protein light chain 3 (LC3) and of p62/SQSTM1. Interestingly, an increased immunoreactivity for p62/SQSTM1 was also observed in hippocampal astrocytes detected in brain slices of TMT-intoxicated rats. The time-lapse recordings of AVs in EGFP-mCherry-LC3B transfected astrocytes demonstrated a reduced mobility of autophagosomes after TMT exposure respect to control cells. The observed block of the autophagic flux cannot be overcome by known autophagy inducers such as rapamycin or 0.5mM lithium. Although ineffective when used at 0.5mM, lithium at higher concentrations (2mM) was able to protect astrocyte cultures from TMT toxicity. This effect correlated well with its ability to determine the phosphorylation/inactivation of glycogen kinase synthase-3β (GSK-3β)

Indicators for the Assessment of Thresholds and Points of Non-Return

Ecological indicators are used increasingly to assess the conditions and/or status of ecosystems. Historically, the first approach was to develop indices based on a particular species or components, e.g. macrophytes, zooplankton, etc. In general, such indices are not broad enough to reflect the complexity of the ecosystem, as they do not include information at the structural, functional and system levels. To cope with these aspects new indices have been develop (for recent reviews see Rapport, 1995; Jørgensen et al., 2005) that try to synthesyze information at all ecosystem levels. In this project we are interested in evaluating indicators in terms of their potential to detect thresholds and point of non-return in coastal ecosystem.JRC.H.5-Rural, water and ecosystem resource

Endocrine Aspects of Environmental "Obesogen" Pollutants

Growing evidence suggests the causal link between the endocrine-disrupting chemicals (EDCs) and the global obesity epidemics, in the context in the so-called "obesogenic environment". Dietary intake of contaminated foods and water, especially in association with unhealthy eating pattern, and inhalation of airborne pollutants represent the major sources of human exposure to EDCs. This is of particular concern in view of the potential impact of obesity on chronic non-transmissible diseases, such as type 2 diabetes, cardiovascular disease, and hormone-sensitive cancers. The key concept is the identification of adipose tissue not only as a preferential site of storage of EDCs, but also as an endocrine organ and, as such, susceptible to endocrine disruption. The timing of exposure to EDCs is critical to the outcome of that exposure, with early lifetime exposures (e.g., fetal or early postnatal) particularly detrimental because of their permanent effects on obesity later in life. Despite that the mechanisms operating in EDCs effects might vary enormously, this minireview is aimed to provide a general overview on the possible association between the pandemics of obesity and EDCs, briefly describing the endocrine mechanisms linking EDCs exposure and latent onset of obesity

Feasibility Study of the Application of the LOICZ Budget to the Mediterranean Sea

The Mediterranean Sea is an important regional EU sea and often used to assess the impacts of global change on the environment, due to its practically enclosed character. As most of the northern part is bordered by the EU, it is also of interest in the evaluation of the environmental and economic impact of the implementation of the EU Water Framework Directive and related environmental legislation. For this purpose, the feasibility to carry out a budget approach, developed in LOICZ (Land Ocean Interactions in the Coastal Zone) project has been analyzed. The activities carried out allow to conclude that all budget components can be reliably estimated and such budget correctly set up. In particular: - estimates for precipitation and evaporation of the Mediterranean Sea can be obtained from ECMWF; other sources for estimates of such variables are available, and in a further implementation of this work a comparison between estimate sets will be carried out; - estimates of annual flow and nutrient output to the Mediterranean Sea as required by the LOICZ budget can be obtained via the setting up of the AVGWLF model, with reasonable operating expense (time and effort) and with acceptable assumptions made for information/data not available for some or all of the regions, especially in the Asian and North African part of the Mediterranean; - exchanges through the Gibraltar and Dardanelles Straits can also be reliably estimated, based on previous studies. It is recognized that the estimates of all budget components, as illustrated in the paragraphs above, can be improved. It is foreseen that some of these improvements will be obtained already in the actual implementation of the LOICZ budget.JRC.H.5-Rural, water and ecosystem resource