The antioxidant and antiproliferative activities of methanolic extracts from Njavara rice bran

<p>Abstract</p> <p>Background</p> <p>Free radical-induced oxidative stress is the root cause for many human diseases. Naturally occurring antioxidant supplements from plants are vital to counter the oxidative damage in cells. The main objective of the present study was to characterize the antioxidant and antiproliferative potential of rice bran extracted from an important Indian rice variety, Njavara and to compare the same with two commercially available basmati rice varieties: Vasumathi, Yamini and a non medicinal variety, Jyothi.</p> <p>Methods</p> <p>Methanolic extracts of rice bran from four varieties; Vasumathi, Yamini, Jyothi and Njavara were used to study their total phenolic and flavonoid contents, <it>in vitro </it>antioxidant activities including total antioxidant activity, scavenging of nitric oxide and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical, reducing power and cytotoxic activity in C6 glioma cells. Correlation coefficient and regression analysis were done by using Sigmastat version 3.1 and Stata statistical package respectively.</p> <p>Results</p> <p>Rice bran methanolic extract from Njavara showed the highest antioxidant and cell cytotoxic properties compared to the other three rice varieties. IC₅₀values for scavenging DPPH and nitric oxide were in the range of 30.85-87.72 μg/ml and 52.25-107.18 μg/ml respectively. Total antioxidant activity and reducing power were increased with increasing amounts of the extract. Total phenolic and flavonoid contents were in the range of 3.2-12.4 mg gallic acid-equivalent (GAE)/g bran and 1.68-8.5 mg quercetin-equivalent (QEE)/g bran respectively. IC₅₀values of cytotoxic assay (MTT assay) were 17.53-57.78 μg/ml. Correlation coefficient and regression analysis of phenolic content with DPPH and NO scavenging, MTT (-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay, total antioxidant assay and reducing power showed a highly significant correlation coefficient values (96-99%) and regression values (91-98%).</p> <p>Conclusion</p> <p>The results of the present study show that the crude methanolic extract from Njavara rice bran contains significantly high polyphenolic compounds with superior antioxidant activity as evidenced by scavenging of free radicals including DPPH and NO. Njavara extracts also showed highest reducing power activity, anti-proliferative property in C6 glioma cells. In conclusion, it is conceivable that the Njavara rice variety could be exploited as one of the potential sources for plant - based pharmaceutical products.</p

Calf health from birth to weaning. I. General aspects of disease prevention

Calfhood diseases have a major impact on the economic viability of cattle operations. This is the first in a three part review series on calf health from birth to weaning, focusing on preventive measures. The review considers both pre- and periparturient management factors influencing calf health, colostrum management in beef and dairy calves and further nutrition and weaning in dairy calves

Island survivors: population genetic structure and demography of the critically endangered giant lizard of La Gomera, Gallotia bravoana

Background: The giant lizard of La Gomera (Gallotia bravoana), is an endemic lacertid of this Canary Island that lives confined to a very restricted area of occupancy in a steep cliff, and is catalogued as Critically Endangered by IUCN. We present the first population genetic analysis of the wild population as well as of captive-born individuals (for which paternity data are available) from a recovery center. Current genetic variability, and inferred past demographic changes were determined in order to discern the relative contribution of natural versus human-mediated effects on the observed decline in population size. Results: Genetic analyses indicate that the only known natural population of the species shows low genetic diversity and acts as a single evolutionary unit. Demographic analyses inferred a prolonged decline of the species for at least 230 generations. Depending on the assumed generation time, the onset of the decline was dated between 1200-13000 years ago. Pedigree analyses of captive individuals suggest that reproductive behavior of the giant lizard of La Gomera may include polyandry, multiple paternity and female long-term sperm retention. Conclusions: The current low genetic diversity of G. bravoana is the result of a long-term gradual decline. Because generation time is unknown in this lizard and estimates had large credibility intervals, it is not possible to determine the relative contribution of humans in the collapse of the population. Shorter generation times would favor a stronger influence of human pressure whereas longer generation times would favor a climate-induced origin of the decline. In any case, our analyses show that the wild population has survived for a long period of time with low levels of genetic diversity and a small effective population size. Reproductive behavior may have acted as an important inbreeding avoidance mechanism allowing the species to elude extinction. Overall, our results suggest that the species retains its adaptive potential and could restore its ancient genetic diversity under favorable conditions. Therefore, management of the giant lizard of La Gomera should concentrate efforts on enhancing population growth rates through captive breeding of the species as well as on restoring the carrying capacity of its natural habitat.Spanish Ministry of Education; European Life Project [LIFE 02 NAT-E-008614]; Ministerio de Ciencia e Innovacion [REN 2001- 1514/GLO, CGL 2010-18216]info:eu-repo/semantics/publishedVersio

Sleep complaints among Brazilian senior citizens from municipalities with different Human Development Indices

PURPOSE: To compare the occurrence of sleep complaints among senior citizens resident in the local communities of two municipalities with differing Human Development Indices (HDIs): Campinas, State of São Paulo (IDH = 0.852) and Parnaíba, State of Piauí (IDH = 0.674). METHOD: Descriptive study as part of the multicentric project going by the name of Frailty among Brazilian Senior Citizens (Fragilidade em Idosos Brasileiros - FIBRA). A total of 988 senior citizens were analysed, making use of a social and demographic questionnaire about sleep problems (Nottingham Health Profile); questions about naps (Minnesota Leisure Activity Questionnaire). The Chi-Square and Mann-Whitney tests were used in the analysis of the data, at a significance level of 5% (pOBJETIVO: comparar la ocurrencia de quejas de sueño en mayores residentes en la comunidad de dos municipios con diferentes Índices de Desarrollo Humano (IDH): Campinas (IDH=0,852) y Parnaíba (IDH=0,674). MÉTODO: estudio descriptivo parte integrante del proyecto multicéntrico Fragilidad en Mayores Brasileños (FIBRA). Fueron evaluados 988 de edad, utilizándose cuestionario sociodemográfico, cuestiones sobre quejas de sueño (Perfil de Salud de Nottingham); cuestiones sobre sueños (Minnesota Leisure Activity Questionnaire). Los testes Jue-cuadrado y Mann Whitney fueron utilizados en el análisis de datos, con nivel de significancia del 5% (pOBJETIVO: comparar a ocorrência de queixas de sono em idosos residentes na comunidade de dois municípios com diferentes índices de desenvolvimento humano (IDH): Campinas, SP, (IDH=0,852) e Parnaíba, PI, (IDH=0,674). MÉTODO: estudo descritivo, parte integrante do projeto multicêntrico Fragilidade em Idosos Brasileiros (Fibra). Foram avaliados 988 idosos, utilizando-se questionário sociodemográfico, questões sobre queixas de sono (Perfil de Saúde de Nottingham); questões sobre cochilo (Minnesota Leisure Activity Questionnaire). Os testes qui-quadrado e Mann-Whitney foram utilizados na análise de dados, com nível de significância de 5% (p<0,05). RESULTADOS: os idosos de Parnaíba apresentaram maiores porcentagens de queixas de sono se comparados aos idosos de Campinas. Houve associação significativa entre município e número de queixas de sono, sono não restaurador, despertar precoce, dificuldade para manter e para iniciar o sono. CONCLUSÃO: os enfermeiros devem intervir na promoção da saúde com ações que minimizem ou previnam esses problemas relativos ao sono