Histochemical and immunohistochemical analyses of primary carcinoma of the liver.

Hematoxylin and eosin (H-E) stained liver sections of 47 autopsy cases of hepatic malignancies were examined. There were 43 cases of hepatocellular carcinoma (subtypes of 30 trabecular, 7 solid, 5 pseudoglandular, and one scirrhous carcinoma), 3 of cholangiocellular carcinoma and one of mixed carcinoma. After immunohistochemical staining, benign hepatocytes reacted positively with anti-epithelial membrane antigen (EMA). Hepatocellular carcinoma cells reacted more weakly than benign hepatocytes. It was noted that the microtubular structure, which could not be demonstrated even by alcian blue or cationic ferric hydroxide colloid stabilized with cacodylate (Fe-CaC), was clearly detected with anti-EMA. The EMA-positive microtubular structures may indicate terminal cholangiolar differentiation. Based on EMA, seven more cases formerly classified as hepatocellular carcinoma by H-E were reclassified as mixed carcinoma, totaling eight (17.0%). The histologic classification of &#34;mixed carcinoma&#34; has been 1.5 to 2.0% of primary liver cancers in Japan, but we suggest there may be more cases of &#34;mixed carcinoma&#34; identified in the future. In conclusion, we emphasize that EMA staining is useful for more accurate classification of hepatic tumors.</p

El cine de Goebbels: una primera aproximación

El presente trabajo pretende investigar la importancia del Ministro de Propaganda Joseph Goebbels en el ámbito cinematográfico y como éste afectó en el pensamiento de la sociedad alemana durante la dictadura nazi y su posterior influencia en la sociedad. Para ello hacemos un breve recorrido por su vida y el contexto histórico en el que transcurre, su dirección en la industria del cine y el análisis de varias películas filmadas durante el gobierno de Adolf Hitler, para comprender y reflexionar sobre la importancia de la propaganda, concretamente cinematográfica, en los Estados totalitarios.Grado en Publicidad y Relaciones Pública

Preponderance of males in special education--why?

The purpose of this study was to determine why there are significantly more males than females in tutorial, remedial, and special education classes today

Aquatically acquired Aeromonas hydrophila wound infection A report of 3 cases

The occurrence of Aeromonas hydrophila wound infections in healthy hosts after water-associated injury is being reported more frequently. This paper reports our experience with 3 such cases and outlines the importance of recognising the association between a water-related injury and, this organism

Biophysical and Biochemical Investigations of Proteins Involved in Genomic Stability

The processes by which cells either make or avoid mutations in their DNA are varied and multifaceted. Often proteins involved in the repair of DNA damage are also found inducing mutations in the immune response. Despite the fact that these proteins and processes are found in all eukaryotes, the scientific community is just beginning to elucidate the mechanisms of these diverse pathways and the functions of the proteins involved in them. This dissertation looks at proteins in two seemingly different cellular processes: DNA mismatch repair and the immune response, as modulated by cytidine deaminases. The DNA mismatch repair protein MutLalpha has been long thought to function as a molecular matchmaker; facilitating interactions between proteins in the mismatch repair pathway. Since little was known about this protein, biophysical and biochemical assays were designed and carried out to elucidate what role binding of ATP and DNA may have on MutLalpha. Surprisingly, MutLalpha can exist in four different conformational states, and these conformational states are governed by adenine nucleotide binding (but not hydrolysis). It had been shown previously that MutLalpha binds to DNA in long, cooperative protein tracts. MutLalpha protein tracts do not appear to be effected by increasing salt concentration, but they are affected by the presence of ATP. Additionally, the ATP hydrolysis rate of full-length MutLalpha was measured, and found to be 2-6x larger than values previously reported for isolated N-terminal domains of MutLalpha, suggesting that conformational changes seen upon ATP binding, and the interactions that are likely made as a result of those conformational changes, may enhance the rate of ATP hydrolysis by MutLalpha. Two of the cytidine deaminases (AID and APOBEC3G) have been shown to be involved in the immune response. The third cytidine deaminase (APOBEC2) is only a cytidine deaminase by sequence homology; and as yet has unknown function. Since it is widely believed that all cytidine deaminases are dimeric or tetrameric, a study of the oligomeric states of these proteins (with and without substrate present) was undertaken. Despite all three proteins belonging to the same superfamily, they each appear to exist in a different oligomeric state

Regional pressure and temperature differences across the injured human brain : comparisons between intraparenchymal and ventricular measurements

Introduction: Intraparenchymal, multimodality sensors are commonly used in the management of patients with severe traumatic brain injury (TBI). The ‘gold standard’, based on accuracy, reliability and cost for intracranial pressure (ICP) monitoring is within the cerebral ventricle (external strain gauge). There are no standards yet for intracerebral temperature monitoring and little is known of temperature differences between brain tissue and ventricle. The aim of the study therefore was to determine pressure and temperature differences at intraparenchymal and ventricular sites during five days of continuous neurominitoring. Methods: Patients with severe TBI requiring emergency surgery. Inclusion criteria: patients who required ICP monitoring were eligible for recruitment. Two intracerebral probe types were used: a) intraventricular, dual parameter sensor (measuring pressure, temperature) with inbuilt catheter for CSF drainage: b) multiparameter intraparenchymal sensor measuring pressure, temperature and oxygen partial pressure. All sensors were inserted during surgery and under aseptic conditions. Results: Seventeen patients, 12 undergoing neurosurgery (decompressive craniectomy n=8, craniotomy n=4) aged 21–78 years were studied. Agreement of measures for 9540 brain tissue-ventricular temperature ‘pairs’ and 10,291 brain tissue-ventricular pressure ‘pairs’ were determined using mixed model to compare mean temperature and pressure for longitudinal data. There was no significant overall difference for mean temperature (p=0.92) or mean pressure readings (p=0.379) between tissue and ventricular sites. With 95.8% of paired temperature readings within 2SD (−0.4 to 0.4°C) differences in temperature between brain tissue and ventricle were clinically insignificant. For pressure, 93.5% of readings pairs fell within the 2SD range (−9.4756 to 7.8112 mmHg) (Fig. 2). However, for individual patients, agreement for mean tissue-ventricular pressure differences was poor on occasions. Conclusions: There is good overall agreement between paired temperature measurements obtained from deep white matter and brain ventricle in patients with and without early neurosurgery. For paired ICP measurements, 93.5% of readings were within 2SD of mean difference. Whilst the majority of paired readings were comparable (within 10mmHg) clinically relevant tissue-ventricular dissociations were noted. Further work is required to unravel the events responsible for short intervals of pressure dissociation before tissue pressure readings can be definitively accepted as a reliable surrogate for ventricular pressure.</p

Elliptic models and M-theory

We give a unified analysis of four-dimensional elliptic models with N=2 supersymmetry and a simple gauge group, and their relation to M-theory. Explicit calculations of the Seiberg-Witten curves and the resulting one-instanton prepotential are presented. The remarkable regularities that emerge are emphasized. In addition, we calculate the prepotential in the Coulomb phase of the (asymptotically-free) Sp(2N) gauge theory with N_f fundamental hypermultiplets of arbitrary mass.Comment: 52 pages, latex, one eps figure, uses psfig.tex; revised version: typos corrected and references adde

Orientación deportiva como actividad extraescolar

En este trabajo de fin de grado lo que pretendo es crear una actividad extraescolar basada en la orientación deportiva, partiendo de la base de que, previamente durante las clases de Educación Física, hayan abordado este tema. Esta actividad les permitirá a los alumnos involucrarse más en la orientación, conocer términos y actividades y sobretodo tener un disfrute y un respeto hacia la naturaleza. El objetivo final que se persigue es que sean capaces de realizar por sí mismos una carrera de orientación programada en el Monte “El Viejo”.Grado en Educación Primari