Comparison of Use of Isoflurane or Sevoflurane for Anaesthesia Induced by Mask in Calves

Background: Inhalation anaesthesia is an effective and reliable general anaesthesia method for inactivity, analgesia, and unconsciousness in extensive surgical procedures. Although widely used, especially in small animals, there are very few studies investigated the reliability and superiority of inhalation anaesthesia in surgical procedures for ruminants. This study intended to evaluate the anaesthetic effects of isoflurane and sevoflurane in different surgical cases with endotracheal intubation following the induction of anaesthesia using a calve-specific facemask, which is not yet a routine option in cattle.Materials, Methods & Results: The study was conducted on 14 calves, from new-born up to 3 months-old, that undergoing various surgical operations. The animals were divided into isoflurane and sevoflurane groups, and each group contained 7 animals. In all cases, atropine (0.04 mg/kg was administered intramusculer as premedication before 15 min from anaesthesia induction. For induction, the anaesthetic agent was given at an inspiratory concentration of 3-5% in the isoflurane group and at an inspiratory concentration of 5 - 7% in the sevoflurane group during 5 min via mask at 15min after atropine administration. In both groups, endotracheal intubation was performed (about 1-3 min) after of general anaesthesia symptoms starting. At 5min after induction, anaesthesia was continued at 1.5 - 3% in the isoflurane group and at 2.5 - 4% in the sevoflurane group during operation. The animals were monitored during anaesthesia and,  anaesthetic effect, reaction of the calve, pronounced side effects, Heart Rate (HR), Systolic Arterial Blood Pressure (SABP), Diastolic Arterial Blood Pressure (DABP), Mean Arterial Blood Pressure (MABP), Respiration Rate (RR), Pulse Rate (PR), Arterial Oxygen Saturation (SpO2) were recorded at before anaesthesia, the premedication period, 5, 15, 30, 45, 60, 75 and 90min of anaesthesia. During anaesthesia, from the clinical parameters, mucosal capillary refilling time was evaluated by applying finger pressure to the oral mucosa. Mucous membrane color was determined by observing the oral mucosa. The time for palpebral reflex and swallowing reflex disappearance was measured and processed into individual forms. Anaesthesia induction and reanimation times were determined and recorded in the forms. After the operation, the return of the swallowing reflex and the time to stand up were recorded. During the operation, urination, defecation, salivation, vomiting, animal reactions, the shape and duration of the reamination period were recorded. In the isoflurane group anaesthesia induction was 3.71 ± 0.28 min, head movements started and came to the sterno- abdominal position at 4.57 ± 0.36 min, got up at 8.71 ± 0.42 min without assistance, meanwhile. In sevoflurane group, anaesthesia induction was 2.57 ± 0.20 min, head movements started and came to the sterno- abdominal position at 3.86 ± 0.40 min, the time to stand up was determined as 6.43 ± 0.29 min. During anaesthesia, HR, SABP, DABP, MABP, RR, PR, SpO2 findings were within physiological acceptable limits in 2 groups. In terms of indicators, there were no obvious differences in either group. The results revealed no significant difference between groups during anaesthesia.Discussion: The isoflurane and sevoflurane anaesthesia used in this study provided an adequate anaesthetic effect in calves characterized by adequate analgesia and muscle relaxation without any complications. The results of our study revealed that there was no significant difference between isoflurane and sevoflurane in calves. Both anaesthetic agents can be used safely for general anaesthesia in calves

Fatty acid and conjugated linoleic acid content of Anatolian buffaloes at different muscle types and slaughter weight

This research was performed to detect tissue fatty acid (FA) composition and conjugated linoleic acid (CLA) content in Anatolian buffaloes at different muscle types (MTs) and slaughter weights (SWs). The research was carried out on a private commercial livestock farm in Tokat. The research's animal material comprised 20 Anatolian buffalo calves with approximately 100 kg body weights, weaned at about 150 days of age. Before the experiment started, the calves were randomly divided into two slaughter groups (SW) as low weight (LW=225 kg) and high weight (HW=325 kg). Ten calves from each of the two experimental groups, which were intensively fed, were slaughtered in two different weights. After the slaughtering, FA composition and CLA content of the Semimembranosus (SM), Semitendinosus (ST), and Triceps brachii (TB) muscle tissues of the animals were examined. The study determined that palmitoleic acid (C16:1) and stearic acid (C18:0) were affected by MTs and oleic acid (C18:1) and alpha-linolenic acid (C18:3 n-3) were affected by SW (P 0.05). With the increase of SW (in LW and HW groups), n-6/n3 (SM: 7.783 and 6.533; ST: 8.115 and 7.859; TB: 8.416 and 8.215) (P > 0.05) and PUFA ratio decreased (P 0.05). Again, with the increase in SW, AI and TI values increased in SM and ST muscles, while the same index values decreased in TB muscle (P> 0.05). In conclusion, when considering the PUFA/SFA ratio and the beneficial effects of CLAs on human health, ST in the MT and LW groups in SW, and thus ST and LW in MT and SW were prominent in Anatolian buffaloes

Lactation curve and persistency of Anatolian buffaloes

WOS: 000360812300003The aim of this study was to determine the lactation curve traits of Anatolian buffaloes raised under different conditions in farms in Tokat Province, Turkey. Wood's gamma curve parameters were employed to identify the lactation curve types, and values for the parameters beginning yield (a), coefficient of rising (b) and coefficient of decreasing (c) were used to determine the shape and type of lactation curve. All parameters in typical lactation curves were positive, and in the event of one parameter being negative, the curve was considered to be an atypical lactation curve. A total of 690 lactation curves were investigated. It was determined that 406 (58.84%) of these curves were typical, while 90 (13.04%) were concave and 194 (28.12%) of a decreasing type. For typical lactation curves, a, b, c, persistency (S), time after parturition until the peak yield occurs (T-max), maximum daily peak yield (Y-max), and coefficient of determination (R-2) were 7.14 +/- 0.008, 0.85 +/- 2.1, 0.40 +/- 0.001, 2.68, 63.6, 6.41 and 76.33, respectively. For concave lactation curves, values for a, b, c, T-max, Y-max and R-2 were 4.94 +/- 0.42, -0.73 +/- 0.016, -0.23 +/- 0.0038, 95.40, 7.41 +/- 0.004 and 71.68, respectively. For decreasing typical lactation curves, values for a, b, c, T-max, and R-2 were 5.31 +/- 0.0041, -0.15 +/- 0.007, 0.039 +/- 0.0023, 3.89 +/- 0.11 and 79.94, respectively. Parameters predicted by the Wood model within the scope of this study have the potential of being useful for breeding programmes. Further breeding/selection activities could be conducted by using the female Anatolian buffaloes with typical lactation curves

The Effect Of Some Environmental Factors On Milk Composition Of Anatolian Buffaloes

WOS: 000413672900011The aim of this study was to determine the composition of raw milk from Anatolian water buffaloes raised under different village conditions in the Tokat province of Northern Turkey. The study materials included 600 milk samples from 120 water buffaloes raised at different farms in 12 separate villages of the Erbaa, Turhal, and Pazar counties in the Tokat Province. The dry matter, nonfat dry matter (or solid non fat), fat, protein, lactose, and casein content of the milk samples were determined. The study results demonstrated that the mean dry matter, nonfat dry matter, fat, protein, lactose, and casein content of the raw milk samples were 16.99 +/- 0.108%, 10.88 +/- 0.036%, 5.98 +/- 0.107%, 4.85 +/- 0.043%, 5.17 +/- 0,021%, and 3.61 +/- 0.036%, respectively. The study data were evaluated according to the water buffaloes' lactation stage, parity, and season by using the SPSS statistical program. It was concluded that the sampling time, parity, village conditions, stage of lactation and calving age had a significant effect (p < 0.05) on the dry matter, nonfat dry matter, fat, protein, lactose, and casein content of raw milk from the Anatolian water buffalo.Ahi Evran University Scientific Research Projects Coordination UnitAhi Evran University [ZRT.E2.16.008]; General Directorate of Agricultural Research and Policies in Tokat provinceThis work was supported by the Ahi Evran University Scientific Research Projects Coordination Unit. Project Number: ZRT.E2.16.008. This study's data were obtained from the scope of the project of improvement of Anatolian buffalo in public hand supported by General Directorate of Agricultural Research and Policies in Tokat province. We would like to thank Tokat Water Buffalo Breeders' Association for valuable technical assistance

Corchorus olitorius and Urtica pilulifera extracts alleviate copper induced oxidative damage and genotoxicity in tomato

Copper cause oxidative damage in plant cells, and plant extracts are the sources of free radical scavengers. We tested the hypothesis that whether Corchorus olitorius (jute) and Urtica pilulifera (Roman nettle) seed extract treatments of germinated seeds affect copper induced oxidative and genotoxic damage or antioxidant response in tomato. Seedlings were exposed to toxic copper concentration (30 ppm) for 7 days. In one experimental group (treatment 1), extract (100 μg mL–1) was added to media. In the other group (treatment 2), tomato seeds were pre-soaked by the extract (100 μg mL–1) prior to germination and copper application. Malondialdehyde and endogenous H2O2 levels in the groups treated with extract and copper were significantly lower than that of the untreated groups. Pre-soaking seeds with the nettle extract solution significantly enhanced catalase activity under unstressed condition. Jute treatment also enhanced catalase activity under copper stress. Ascorbate peroxidase activity remained at unstressed level in copper treated groups. Extract treatments significantly decreased copper induced DNA damage in root nuclei. Jute seed extract contained salicylic acid and quercetin which can be correlated with the evoked effects. We demonstrated protective effect of plant extract treatments against copper stress of tomato seedlings prior to germination or during seedling development

Antenatal screening and its possible meaning from unborn baby's perspective

In recent decades antenatal screening has become one of the most routine procedure of pregnancy-follow up and the subject of hot debate in bioethics circles. In this paper the rationale behind doing antenatal screening and the actual and potential problems that it may cause will be discussed. The paper will examine the issue from the point of wiew of parents, health care professionals and, most importantly, the child-to-be. It will show how unthoughtfully antenatal screening is performed and how pregnancy is treated almost as a disease just since the emergence of antenatal screening. Genetic screening and ethical problems caused by the procedure will also be addressed and I will suggest that screening is more to do with the interests of others rather than those of the child-to be

Fatty acid and conjugated linoleic acid content of Anatolian buffaloes at different muscle types and slaughter weight

This research was performed to detect tissue fatty acid (FA) composition and conjugated linoleic acid (CLA) content in Anatolian buffaloes at different muscle types (MTs) and slaughter weights (SWs). The research was carried out on a private commercial livestock farm in Tokat. The research's animal material comprised 20 Anatolian buffalo calves with approximately 100 kg body weights, weaned at about 150 days of age. Before the experiment started, the calves were randomly divided into two slaughter groups (SW) as low weight (LW=225 kg) and high weight (HW=325 kg). Ten calves from each of the two experimental groups, which were intensively fed, were slaughtered in two different weights. After the slaughtering, FA composition and CLA content of the Semimembranosus (SM), Semitendinosus (ST), and Triceps brachii (TB) muscle tissues of the animals were examined. The study determined that palmitoleic acid (C16:1) and stearic acid (C18:0) were affected by MTs and oleic acid (C18:1) and alpha-linolenic acid (C18:3 n-3) were affected by SW (P 0.05). With the increase of SW (in LW and HW groups), n-6/n3 (SM: 7.783 and 6.533; ST: 8.115 and 7.859; TB: 8.416 and 8.215) (P > 0.05) and PUFA ratio decreased (P 0.05). Again, with the increase in SW, AI and TI values increased in SM and ST muscles, while the same index values decreased in TB muscle (P> 0.05). In conclusion, when considering the PUFA/SFA ratio and the beneficial effects of CLAs on human health, ST in the MT and LW groups in SW, and thus ST and LW in MT and SW were prominent in Anatolian buffaloes.Scientific and Technological Research Council of Turkiye [TUBITAK-114O710]All of the authors of this manuscript thank the Scientific and Technological Research Council of Turkiye. The data were collected during the execution of the project supported by the Scientific and Technological Research Council of Turkiye (grant no. TUBITAK-114O710)

The 'four principles of bioethics' as found in 13(th) century Muslim scholar Mawlana's teachings

BACKGROUND: There have been different ethical approaches to the issues in the history of philosophy. Two American philosophers Beachump and Childress formulated some ethical principles namely 'respect to autonomy', 'justice', 'beneficence' and 'non-maleficence'. These 'Four Principles' were presented by the authors as universal and applicable to any culture and society. Mawlana, a great figure in Sufi tradition, had written many books which not only guide people how to worship God to be close to Him, but also advise people how to lead a good life to enrich their personality, as well as to create a harmonious society and a peaceful world. METHODS: In this study we examined the major works of Mawlana to find out which of these 'Four Principles of Bioethics' exist in Mawlana's ethical understanding. RESULTS: We have found in our study that all these principles exist in Mawlana's writings and philosophy in one form or another. CONCLUSIONS: We have concluded that, further to Beachump and Childress' claim that these principles are universal and applicable to any culture and society, these principles have always existed in different moral traditions in different ways, of which Mawlana's teaching might be presented as a good example